New steroidal saponins from the aerial parts of Paris polyphylla var. yunnanensis and their effects on blood coagulation
Fitoterapia. 2024 Jan 30;174:105833. doi: 10.1016/j.fitote.2024.105833. Online ahead of print.ABSTRACTFive new steroidal saponins, paripolins D-H (1-5), and 6 known compounds (6-11) were isolated from the aerial parts of Paris polyphylla var. yunnanensis. The structures of 1-5 were determined using spectroscopic analyses in conjunction with acid hydrolysis. It is for the first time to report the 12-hydroxysteroidal saponins from the genus Paris. The effect of all isolated compounds on blood coagulation was determined in vitro using the plasma recalcification time method. Compounds 1 and 2 showed potent procoagulant activit...
Source: Fitoterapia - February 1, 2024 Category: Biochemistry Authors: Wei Nie Hong-Yan Zhang Yan-Xia Ma Jin-Fu Wan Li-Ping Feng Tao Cui Qian Li Source Type: research
Chemical characterization of three different extracts obtained from Chelidonium majus L. (Greater celandine) with insights into their in vitro, in silico and network pharmacological properties
Fitoterapia. 2024 Jan 30;174:105835. doi: 10.1016/j.fitote.2024.105835. Online ahead of print.ABSTRACTPlant species C. majus, which is a very rich source of secondary metabolites, was used to obtain extracts, using a conventional extraction technique. For the extraction of bioactive molecules, three solvents were used: ethyl acetate, methanol and water, which differ from each other based on their polarity. The obtained extracts were examined in terms of chemical composition, antioxidant, enzyme inhibitory activity, and cytotoxic effects. The research results indicate that methanol was a better and more efficient extractant...
Source: Fitoterapia - February 1, 2024 Category: Biochemistry Authors: Milena Terzic Shaimaa Fayez Nouran M Fahmy Omayma A Eldahshan Abdullahi Ibrahim Uba Sathish Kumar M Ponniya Selami Selvi None Nilofar Ismail Koyuncu Özgür Yüksekdağ Gokhan Zengin Source Type: research
New indolequinazoline alkaloids from the fruits of Tetradium ruticarpum
Fitoterapia. 2024 Jan 30;174:105843. doi: 10.1016/j.fitote.2024.105843. Online ahead of print.ABSTRACTIn this research, five new indolequinazoline alkaloids (1-5), along with six known indolequinazoline alkaloids (6-11) were obtained from the fruits of Tetradium ruticarpum. Their structures were elucidated through comprehensive spectroscopic data of 1D and 2D NMR, HRESIMS and ECD spectra. Additionally, all isolates were assayed for their SIRT1 inhibitory activities in vitro and compounds 2, 7, 10 and 11 exhibited activities with IC50 values ranged from 43.16 to 118.35 μM.PMID:38301937 | DOI:10.1016/j.fitote.2024.105843 (Source: Fitoterapia)
Source: Fitoterapia - February 1, 2024 Category: Biochemistry Authors: Mu Li Xin-Lin Chen Zhan-Peng Yu Ya-Lin Li Li-Hong Hong Yu-Xing Cai Ling-Yi Kong Jian-Guang Luo Source Type: research
Diverse diterpenoids and a triterpenoid from Euphorbia spinidens Bornm. ex Prokh
Fitoterapia. 2024 Mar;173:105838. doi: 10.1016/j.fitote.2024.105838. Epub 2024 Jan 29.ABSTRACTFour previously unreported diterpenoids including three ent-atisanes (1-3) and one ent-abietane (4), along with one known linear triterpenoid (5) and five known diterpenoids including four myrsinanes (6-9), and one abietane (10) have been isolated from the roots of Euphorbia spinidens Bornm. ex Prokh. The structures were determined on the basis of extensive spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR and comparison of the data with those reported in the literature. Antimicrobial potential of isolated compounds were a...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: Abolfazl Shakeri Mohammad Reza Mirahmadi Olaf Kunert Yu-Chi Tsai Anita Barta Judit Hohmann Javad Asili Source Type: research
Five new secondary metabolites from Aster tataricus
Fitoterapia. 2024 Feb 1;174:105828. doi: 10.1016/j.fitote.2024.105828. Online ahead of print.ABSTRACTAster tataricus L.f. is highly valued for its rich reserves of bioactive compounds. Our research focused on the identification of previously unreported compounds found within the ethanol extract of A. tataricus. Through meticulous spectroscopic analyses and computational methods like NMR calculations and ECD, we successfully elucidated the structures of five novel compounds termed tatarisides A-E (1-5), alongside two known compounds (6, 7). The anti-inflammatory assays conducted yielded noteworthy results, particularly in r...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: Jian-Li Li Chao Chen Min-Jie Ma Pei Li Si-Qi Yang Rui Guo Chun-Lei Zhang Yan-Gang Cheng Source Type: research
A new integrated strategy for high purity pinolenic acid production from Pinus koraiensis Sieb. et Zucc seed oil and evaluation of its hypolipidemic activity in vivo
Fitoterapia. 2024 Jan 29:105842. doi: 10.1016/j.fitote.2024.105842. Online ahead of print.ABSTRACTPinolenic acid is a polyunsaturated fatty acid present only in Pinus koraiensis Sieb. et Zucc seed oil. In order to solve the structural instability problem of polyunsaturated fatty acids, pinolenic acid of P. koraiensis seed oil was effectively isolated and purified by the integrated strategy of ethyl esterification followed by urea inclusion for the first time. Under the optimal conditions after the Box-Benhnken Design experimental, ethyl pinolenate with high purity 94.95% could be obtained, and the average content of PNAEE ...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: Linan Sun Maoyu Zhang Xiaodan Wu Sansan Bao Liang Yu Ying Wang Yujie Fu Source Type: research
Phytochemical analysis and antihyperglycemic activity of Castilleja arvensis
Fitoterapia. 2024 Jan 29;174:105839. doi: 10.1016/j.fitote.2024.105839. Online ahead of print.ABSTRACTCastilleja genus comprises approximately 211 species, some of them exhibiting potential in treating various diseases. Remarkably, despite its abundance, there is a significant lack of scientific studies that explore the chemical composition and/or therapeutic activity of this genus. In this work, the chemical composition of Castilleja arvensis was determined, and its antihyperglycemic activity was evaluated in vivo, in vitro, and ex vivo. Hydroalcoholic extract of C. arvensis (HECa) was obtained from the maceration of aeri...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: M ónica Aideé Díaz-Román Juan Jos é Acevedo-Fernández Gabriela Ávila-Villarreal Elizabeth Negrete-Le ón A Berenice Aguilar-Guadarrama Source Type: research
Multi-level chemical characterization and anti-inflammatory activity evaluation of the polysaccharides from Prunella vulgaris
Fitoterapia. 2024 Jan 29;174:105841. doi: 10.1016/j.fitote.2024.105841. Online ahead of print.ABSTRACTPrunella vulgaris (PV) is a medicine and food homologous plant, but its quality evaluation seldom relies on the polysaccharides (PVPs). In this work, we established the multi-level fingerprinting and in vitro anti-inflammatory evaluation approaches to characterize and compare the polysaccharides of P. vulgaris collected from the major production regions in China. PVPs prepared from 22 batches of samples gave the content variation of 5.76-24.524 mg/g, but displayed high similarity in the molecular weight distribution. Hydro...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: He Sun Jia Lou Bo-Xue Chen Jia-Qi Huang Qi-Long Wang Shao-Fei Song Zi-Yue Jia Rong Miao Shi-Yu Wang Xue Li Wen-Zhi Yang Source Type: research
Dimeric ent-kauranoids isolated from Isodon japonica var. Glaucocalyx and their anti-inflammatory activities
Fitoterapia. 2024 Jan 29;174:105840. doi: 10.1016/j.fitote.2024.105840. Online ahead of print.ABSTRACTThe phytochemical investigation of the aerial parts of Isodon japonica var. glaucocalyx afforded four undescribed (glaucocalyxin O-R, 1-4) and six known ent-kauranoids (5-10). Their structures were established using NMR and MS measurements. Compounds 1 and 2 are dimeric ent-kaurane-type diterpenoids. Moreover, the plausible biogenetic pathways for compounds 1 and 2 were proposed as Michael addition between two monomers. Eight compounds were assayed for their anti-inflammatory activity by evaluating NO production in LPS-ind...
Source: Fitoterapia - January 31, 2024 Category: Biochemistry Authors: Ge Wang Ao-Xue Ding Guo-Qing Qin Tong Chen Xiang-Gang Hu Liu Zheng Gao-Xiang Du Wenqiong Wang Lijiang Xuan Source Type: research
Research on the antipruritic active ingredients of Mikania micrantha
This study indicated that the main antipruritic active ingredients of Mikania micrantha are β-caryophyllene and humulene.PMID:38286314 | DOI:10.1016/j.fitote.2024.105837 (Source: Fitoterapia)
Source: Fitoterapia - January 29, 2024 Category: Biochemistry Authors: Qianqian Cheng Bin Lyu Jingweng Hu Zhiqiang Zhang Yusheng Huang Zhenhua Wang Source Type: research
Citrisorbicillinol, an undescribed hybrid sorbicillinoid with osteogenic activity from Penicillium citrinum ZY-2
Fitoterapia. 2024 Mar;173:105836. doi: 10.1016/j.fitote.2024.105836. Epub 2024 Jan 28.ABSTRACTCitrisorbicillinol (1), along with six other known compounds (2-7), was isolated from an endphyte Penicillium citrinum ZY-2 of Plantago asiatica L. Citrisorbicillinol (1) was characterized as a skeletally unprecedented hybrid sorbicillinoid, and its unique framework is likely formed by intermolecular [4 + 2] cycloaddition between intermediates derived from citrinin and sorbicillinoid biosynthetic gene clusters. Compounds 1 and 2 demonstrated to promote osteoblastic differentiation in MC3T3-E1 cells, and to be osteogenic in the pre...
Source: Fitoterapia - January 29, 2024 Category: Biochemistry Authors: Ying Zhang Jing Zhang Qi Du Xue Ming Wu Yong Chen Ren Xiang Tan Source Type: research
Phytochemistry and pharmacological activities of five species of Bauhinia genus: A review
In conclusion, the pharmacological potential of Bauhinia plants show promise with various leads such as insulin-like protein, roseoside, bauhiniastatin, and melibiose binding lectin. However, further investigations are required to address existing gaps and advance them towards product development. This review will lay the groundwork for future research initiatives aimed at fully realizing the therapeutic potential of Bauhinia plants.PMID:38286316 | DOI:10.1016/j.fitote.2024.105830 (Source: Fitoterapia)
Source: Fitoterapia - January 29, 2024 Category: Biochemistry Authors: Divya Gudavalli Komal Pandey Venkata Gopal Ede Dakshata Sable Ashwini S Ghagare Abhijeet S Kate Source Type: research
UPLC-Q-TOF/MS based metabolite profiling and quality marker constituents screening of root, stem and rhizome extracts of Ilex asprella
CONCLUSION: A sensitive and rapid strategy was established to evaluate the differences on secondary metabolites of different parts of IA for the first time, and this study may contribute to the quality evaluation of medicinal herbs and provide theoretically data support for further analysis of different parts of IA.PMID:38280682 | DOI:10.1016/j.fitote.2024.105832 (Source: Fitoterapia)
Source: Fitoterapia - January 27, 2024 Category: Biochemistry Authors: Lulu Wang Zhechen Fan Siyi Ma Shiyu Wu Chi Ma Huawu Zeng Xike Xu Qing Ma Ji Ye Source Type: research
New cassane-type alkaloids and diterpenoids from the pericarps of Caesalpinia bonduc
Fitoterapia. 2024 Mar;173:105834. doi: 10.1016/j.fitote.2024.105834. Epub 2024 Jan 26.ABSTRACTThe phytochemical investigation of the pericarps of Caesalpinia bonduc led to the isolation and identification of five new cassane-type alkaloids: caesalminines C - G (1-5) and six new diterpenoids: caesalbonducin K - P (6-11), along with seven known compounds (12-18). Compounds 1-5 were identified as a group of rare alkaloids possessing a tetracyclic cassane-type diterpenoid skeleton with a lactam D-ring instead of a typical furan or lactone moiety. The structures of 1-11 were elucidated on the basis of 1D and 2D NMR including HS...
Source: Fitoterapia - January 27, 2024 Category: Biochemistry Authors: Shuai-Jie Yan Si-Yang Huang Jia-Shuo Xing Yang-Rui Cai Ye-Ping Ruan Pan-Pan Zhang Source Type: research
Advanced quality assessment of Sanshishi (Gardenia jasminoides Ellis) and Kampo medicines using a monoclonal antibody against geniposide
This study developed such a method by first synthesizing GE-bovine serum albumin conjugates to function as immunizing agents in mice. This led to the production of a monoclonal antibody (mAb 3A6) against GE from the fusion of splenocytes from immunized mice with myeloma cells (P3U1), resulting in a hybridoma that produces mAb 3A6. Thereafter, we developed a mAb 3A6-based indirect competitive enzyme-linked immunosorbent assay (icELISA). The icELISA exhibited satisfactory sensitivity (0.391-12.5 μg/ml) and repeatability (coefficients of variation <10%). The accuracy of this method was validated through a spike-recovery a...
Source: Fitoterapia - January 26, 2024 Category: Biochemistry Authors: Kanta Noguchi Daisuke Imahori Riko Nishiura Poomraphie Nuntawong Waraporn Putalun Hiroyuki Tanaka Satoshi Morimoto Seiichi Sakamoto Source Type: research
