Ndfip1 protected dopaminergic neurons via regulating mitochondrial function and ferroptosis in Parkinson's disease
Exp Neurol. 2024 Feb 15;375:114724. doi: 10.1016/j.expneurol.2024.114724. Online ahead of print.ABSTRACTIncreasing evidence has shown that mitochondrial dysfunction and iron accumulation contribute to the pathogenesis of Parkinson's disease (PD). Nedd4 family interacting protein 1 (Ndfip1) is an adaptor protein of the Nedd4 E3 ubiquitin ligases. We have previously reported that Ndfip1 showed a neuroprotective effect in cell models of PD. However, whether Ndfip1 could protect dopaminergic neurons in PD animal models in vivo and the possible mechanisms are not known. Here, our results showed that the expression of Ndfip1 dec...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Xiaomin Fu Le Qu Huamin Xu Junxia Xie Source Type: research
PI3K δ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner
In this study, a rodent ischemia/reperfusion model was established in C57BL/6 mice via transient middle cerebral artery occlusion and reperfusion (MCAO/R). The PI3Kδ expression in ischemic penumbra was remarkably upregulated following MCAO/R induction. PI3Kδ inhibitor CAL-101 improved the CBF recovery, ischemic brain injury, and suppressed capillary constriction in MCAO/R mice. Besides, the production of tumor necrosis factor alpha (TNF-α), an inducer for tissue injury, and the expression of transient receptor potential vanilloid type 2 (TRPV2), a channel protein permitting calcium (Ca2+) uptake, were significantly redu...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Jing Qiu Yi-Han Wang Xiu-Ming Wang Hui-Sheng Chen Source Type: research
Social isolation initiated post-weaning augments ischemic brain injury by promoting pro-inflammatory responses
Exp Neurol. 2024 Feb 15;375:114729. doi: 10.1016/j.expneurol.2024.114729. Online ahead of print.ABSTRACTSocial isolation is associated with poor stroke outcome, but the underlying molecular mechanisms were largely unknown. In male Balb/C mice exposed to transient middle cerebral artery occlusion (MCAo), we examined the effects of social isolation initiated post-weaning on ischemic injury, cytokine/chemokine responses and cell signaling using a broad panel of techniques that involved immunocytochemistry, cytokine/chemokine array and Western blots. Social isolation initiated post-weaning elevated infarct size, brain edema an...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Muhammed Furkan Dasdelen Ahmet Burak Caglayan Sezgin Er Mustafa Caglar Beker Nilay Ates Janine Gronewold Thorsten Roland Doeppner Dirk M Hermann Ertugrul Kilic Source Type: research
Effects of dim light at night in C57BL/6 J mice on recovery after spinal cord injury
Exp Neurol. 2024 Feb 14:114725. doi: 10.1016/j.expneurol.2024.114725. Online ahead of print.ABSTRACTSpinal cord injury (SCI) can cause long-lasting locomotor deficits, pain, and mood disorders. Anatomical and functional outcomes are exacerbated by inflammation after SCI, which causes secondary damage. One promising target after SCI is manipulating the circadian system, which optimizes biology and behavior for time of day - including neuroimmune responses and mood-related behaviors. Circadian disruption after SCI is likely worsened by a disruptive hospital environment, which typically includes dim light-at-night (dLAN). Her...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: John C Aldrich Ashley R Scheinfeld Sydney E Lee Kalina J Dusenbery Kathryn M Mahach Brigid C Van de Veire Laura K Fonken Andrew D Gaudet Source Type: research
Ndfip1 protected dopaminergic neurons via regulating mitochondrial function and ferroptosis in Parkinson's disease
Exp Neurol. 2024 Feb 14:114724. doi: 10.1016/j.expneurol.2024.114724. Online ahead of print.ABSTRACTIncreasing evidence has shown that mitochondrial dysfunction and iron accumulation contribute to the pathogenesis of Parkinson's disease (PD). Nedd4 family interacting protein 1 (Ndfip1) is an adaptor protein of the Nedd4 E3 ubiquitin ligases. We have previously reported that Ndfip1 showed a neuroprotective effect in cell models of PD. However, whether Ndfip1 could protect dopaminergic neurons in PD animal models in vivo and the possible mechanisms are not known. Here, our results showed that the expression of Ndfip1 decreas...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Xiaomin Fu Le Qu Huamin Xu Junxia Xie Source Type: research
PI3K δ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner
In this study, a rodent ischemia/reperfusion model was established in C57BL/6 mice via transient middle cerebral artery occlusion and reperfusion (MCAO/R). The PI3Kδ expression in ischemic penumbra was remarkably upregulated following MCAO/R induction. PI3Kδ inhibitor CAL-101 improved the CBF recovery, ischemic brain injury, and suppressed capillary constriction in MCAO/R mice. Besides, the production of tumor necrosis factor alpha (TNF-α), an inducer for tissue injury, and the expression of transient receptor potential vanilloid type 2 (TRPV2), a channel protein permitting calcium (Ca2+) uptake, were significantly redu...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Jing Qiu Yi-Han Wang Xiu-Ming Wang Hui-Sheng Chen Source Type: research
Social isolation initiated post-weaning augments ischemic brain injury by promoting pro-inflammatory responses
Exp Neurol. 2024 Feb 15;375:114729. doi: 10.1016/j.expneurol.2024.114729. Online ahead of print.ABSTRACTSocial isolation is associated with poor stroke outcome, but the underlying molecular mechanisms were largely unknown. In male Balb/C mice exposed to transient middle cerebral artery occlusion (MCAo), we examined the effects of social isolation initiated post-weaning on ischemic injury, cytokine/chemokine responses and cell signaling using a broad panel of techniques that involved immunocytochemistry, cytokine/chemokine array and Western blots. Social isolation initiated post-weaning elevated infarct size, brain edema an...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Muhammed Furkan Dasdelen Ahmet Burak Caglayan Sezgin Er Mustafa Caglar Beker Nilay Ates Janine Gronewold Thorsten Roland Doeppner Dirk M Hermann Ertugrul Kilic Source Type: research
Effects of dim light at night in C57BL/6 J mice on recovery after spinal cord injury
Exp Neurol. 2024 Feb 14:114725. doi: 10.1016/j.expneurol.2024.114725. Online ahead of print.ABSTRACTSpinal cord injury (SCI) can cause long-lasting locomotor deficits, pain, and mood disorders. Anatomical and functional outcomes are exacerbated by inflammation after SCI, which causes secondary damage. One promising target after SCI is manipulating the circadian system, which optimizes biology and behavior for time of day - including neuroimmune responses and mood-related behaviors. Circadian disruption after SCI is likely worsened by a disruptive hospital environment, which typically includes dim light-at-night (dLAN). Her...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: John C Aldrich Ashley R Scheinfeld Sydney E Lee Kalina J Dusenbery Kathryn M Mahach Brigid C Van de Veire Laura K Fonken Andrew D Gaudet Source Type: research
Ndfip1 protected dopaminergic neurons via regulating mitochondrial function and ferroptosis in Parkinson's disease
Exp Neurol. 2024 Feb 14:114724. doi: 10.1016/j.expneurol.2024.114724. Online ahead of print.ABSTRACTIncreasing evidence has shown that mitochondrial dysfunction and iron accumulation contribute to the pathogenesis of Parkinson's disease (PD). Nedd4 family interacting protein 1 (Ndfip1) is an adaptor protein of the Nedd4 E3 ubiquitin ligases. We have previously reported that Ndfip1 showed a neuroprotective effect in cell models of PD. However, whether Ndfip1 could protect dopaminergic neurons in PD animal models in vivo and the possible mechanisms are not known. Here, our results showed that the expression of Ndfip1 decreas...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Xiaomin Fu Le Qu Huamin Xu Junxia Xie Source Type: research
PI3K δ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner
In this study, a rodent ischemia/reperfusion model was established in C57BL/6 mice via transient middle cerebral artery occlusion and reperfusion (MCAO/R). The PI3Kδ expression in ischemic penumbra was remarkably upregulated following MCAO/R induction. PI3Kδ inhibitor CAL-101 improved the CBF recovery, ischemic brain injury, and suppressed capillary constriction in MCAO/R mice. Besides, the production of tumor necrosis factor alpha (TNF-α), an inducer for tissue injury, and the expression of transient receptor potential vanilloid type 2 (TRPV2), a channel protein permitting calcium (Ca2+) uptake, were significantly redu...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Jing Qiu Yi-Han Wang Xiu-Ming Wang Hui-Sheng Chen Source Type: research
Social isolation initiated post-weaning augments ischemic brain injury by promoting pro-inflammatory responses
Exp Neurol. 2024 Feb 15;375:114729. doi: 10.1016/j.expneurol.2024.114729. Online ahead of print.ABSTRACTSocial isolation is associated with poor stroke outcome, but the underlying molecular mechanisms were largely unknown. In male Balb/C mice exposed to transient middle cerebral artery occlusion (MCAo), we examined the effects of social isolation initiated post-weaning on ischemic injury, cytokine/chemokine responses and cell signaling using a broad panel of techniques that involved immunocytochemistry, cytokine/chemokine array and Western blots. Social isolation initiated post-weaning elevated infarct size, brain edema an...
Source: Experimental Neurology - February 16, 2024 Category: Neurology Authors: Muhammed Furkan Dasdelen Ahmet Burak Caglayan Sezgin Er Mustafa Caglar Beker Nilay Ates Janine Gronewold Thorsten Roland Doeppner Dirk M Hermann Ertugrul Kilic Source Type: research
The paradox of tPA in ischemic stroke: tPA knockdown following recanalization improves functional and histological outcomes
In conclusion, our results provide evidence that reducing endogenous tPA following recanalization improves functional outcomes and reduces post-stroke brain damage.PMID:38360257 | DOI:10.1016/j.expneurol.2024.114727 (Source: Experimental Neurology)
Source: Experimental Neurology - February 15, 2024 Category: Neurology Authors: Siva Reddy Challa Koteswara Rao Nalamolu Casimir A Fornal Isidra M Baker Adithya Mohandass Sahil Reddy Mada Billy C Wang David M Pinson Sourabh Lahoti Jeffrey D Klopfenstein Krishna Kumar Veeravalli Source Type: research
The paradox of tPA in ischemic stroke: tPA knockdown following recanalization improves functional and histological outcomes
In conclusion, our results provide evidence that reducing endogenous tPA following recanalization improves functional outcomes and reduces post-stroke brain damage.PMID:38360257 | DOI:10.1016/j.expneurol.2024.114727 (Source: Experimental Neurology)
Source: Experimental Neurology - February 15, 2024 Category: Neurology Authors: Siva Reddy Challa Koteswara Rao Nalamolu Casimir A Fornal Isidra M Baker Adithya Mohandass Sahil Reddy Mada Billy C Wang David M Pinson Sourabh Lahoti Jeffrey D Klopfenstein Krishna Kumar Veeravalli Source Type: research
microRNA-9a-5p disrupts the ELAVL1/VEGF axis to alleviate traumatic brain injury
This study aims to investigate the possible role and mechanisms of miR-9a-5p affecting TBI. Microarray-based gene expression profiling of TBI was used for screening differentially expressed miRNAs and genes. TBI rat models were established. miR-9a-5p, ELAVL1 and VEGF expression in the brain tissue of TBI rats was detected. The relationship among miR-9a-5p, ELAVL1 and VEGF was tested. TBI modeled rats were injected with miR-9a-5p-, ELAVL1 or VEGF-related sequences to identify their effects on TBI. miR-9a-5p was poorly expressed in the brain tissue of rats with TBI. ELAVL1 was a downstream target gene of miR-9a-5p, which cou...
Source: Experimental Neurology - February 11, 2024 Category: Neurology Authors: Chenxi Feng Qiuyan Tian Xiaojuan Tang Jian Yu Hong Li Changxing Geng Lixiao Xu Source Type: research
To evaluate the effect of endothelin receptor agonist IRL-1620 alone and in combination with donepezil in modulating neurodegeneration elicited by amyloid- β in rats
Exp Neurol. 2024 Feb 9:114720. doi: 10.1016/j.expneurol.2024.114720. Online ahead of print.ABSTRACTThe creation of efficient therapies for Alzheimer's disease is essential since it is a serious public health problem. This investigation sought to ascertain any potential synergistic benefits of treating Alzheimer's disease with IRL-1620 monotherapy in addition to Donepezil. Additionally, the toxicity profile of IRL-1620 was evaluated using different doses (5 μg/kg,7 μg/kg,9 μg/kg). The study further assessed neurobehavioral, biochemical, molecular, and histopathological parameters to evaluate the efficacy of both IRL1620 ...
Source: Experimental Neurology - February 11, 2024 Category: Neurology Authors: Mahajan Eshani Raja Anupam Sharma Amit Raj Jain Ashish K Prabha Praisy Prakash Ajay Medhi Bikash Source Type: research
