An efficient evaluation system for factors affecting the genome editing efficiency in mouse
In this study, we established a system that can easily determine the genotype at the mouse (Mus musculus) Tyr gene locus for genome editing both in vitro and in vivo. In this genome editing system, by designing the Cas9 cleavage site and donor template, wild-type, knockout, and knock-in genotypes can be distinguished by restriction fragment length polymorphisms of PCR products. Moreover, the introduction of the H420R mutation in tyrosinase allows the determination of knock-in mice with specific coat color patterns. Using this system, we evaluated the effects of small-molecule compounds on the efficiency of genome editing i...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Yusuke Sakai Yuri Okabe Gen Itai Seiji Shiozawa Source Type: research
Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36
In this study, we have successfully generated a novel CD36 humanized mouse strain where the sequences encoding the extracellular domains of the mouse Cd36 gene were replaced with the corresponding human sequences. The results showed that CD36 humanized mice only expressed human CD36, and the proportion of each lymphocyte was not significantly changed compared with wild-type mice. Furthermore, CD36 monoclonal antibody could significantly inhibit tumor growth after treatment. Therefore, the CD36 humanized mice represent a validated preclinical mouse model for the evaluation of tumor immunotherapy targeting CD36.PMID:37407484...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Xiulong Xie Zhenlan Niu Linlin Wang Xiaofei Zhou Xingyan Yu Hongyan Jing Yi Yang Source Type: research
An efficient evaluation system for factors affecting the genome editing efficiency in mouse
In this study, we established a system that can easily determine the genotype at the mouse (Mus musculus) Tyr gene locus for genome editing both in vitro and in vivo. In this genome editing system, by designing the Cas9 cleavage site and donor template, wild-type, knockout, and knock-in genotypes can be distinguished by restriction fragment length polymorphisms of PCR products. Moreover, the introduction of the H420R mutation in tyrosinase allows the determination of knock-in mice with specific coat color patterns. Using this system, we evaluated the effects of small-molecule compounds on the efficiency of genome editing i...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Yusuke Sakai Yuri Okabe Gen Itai Seiji Shiozawa Source Type: research
Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36
In this study, we have successfully generated a novel CD36 humanized mouse strain where the sequences encoding the extracellular domains of the mouse Cd36 gene were replaced with the corresponding human sequences. The results showed that CD36 humanized mice only expressed human CD36, and the proportion of each lymphocyte was not significantly changed compared with wild-type mice. Furthermore, CD36 monoclonal antibody could significantly inhibit tumor growth after treatment. Therefore, the CD36 humanized mice represent a validated preclinical mouse model for the evaluation of tumor immunotherapy targeting CD36.PMID:37407484...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Xiulong Xie Zhenlan Niu Linlin Wang Xiaofei Zhou Xingyan Yu Hongyan Jing Yi Yang Source Type: research
An efficient evaluation system for factors affecting the genome editing efficiency in mouse
In this study, we established a system that can easily determine the genotype at the mouse (Mus musculus) Tyr gene locus for genome editing both in vitro and in vivo. In this genome editing system, by designing the Cas9 cleavage site and donor template, wild-type, knockout, and knock-in genotypes can be distinguished by restriction fragment length polymorphisms of PCR products. Moreover, the introduction of the H420R mutation in tyrosinase allows the determination of knock-in mice with specific coat color patterns. Using this system, we evaluated the effects of small-molecule compounds on the efficiency of genome editing i...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Yusuke Sakai Yuri Okabe Gen Itai Seiji Shiozawa Source Type: research
Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36
In this study, we have successfully generated a novel CD36 humanized mouse strain where the sequences encoding the extracellular domains of the mouse Cd36 gene were replaced with the corresponding human sequences. The results showed that CD36 humanized mice only expressed human CD36, and the proportion of each lymphocyte was not significantly changed compared with wild-type mice. Furthermore, CD36 monoclonal antibody could significantly inhibit tumor growth after treatment. Therefore, the CD36 humanized mice represent a validated preclinical mouse model for the evaluation of tumor immunotherapy targeting CD36.PMID:37407484...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Xiulong Xie Zhenlan Niu Linlin Wang Xiaofei Zhou Xingyan Yu Hongyan Jing Yi Yang Source Type: research
An efficient evaluation system for factors affecting the genome editing efficiency in mouse
In this study, we established a system that can easily determine the genotype at the mouse (Mus musculus) Tyr gene locus for genome editing both in vitro and in vivo. In this genome editing system, by designing the Cas9 cleavage site and donor template, wild-type, knockout, and knock-in genotypes can be distinguished by restriction fragment length polymorphisms of PCR products. Moreover, the introduction of the H420R mutation in tyrosinase allows the determination of knock-in mice with specific coat color patterns. Using this system, we evaluated the effects of small-molecule compounds on the efficiency of genome editing i...
Source: Experimental Animals - July 5, 2023 Category: Research Authors: Yusuke Sakai Yuri Okabe Gen Itai Seiji Shiozawa Source Type: research
Differences in susceptibility to ADR nephropathy among C57BL/6 substrains
Exp Anim. 2023 Jun 21. doi: 10.1538/expanim.23-0003. Online ahead of print.ABSTRACTAdriamycin (ADR) nephropathy is the most widely used nephropathy model to study the pathophysiological mechanisms of chronic kidney disease (CKD) in mice. However, its application is limited to a few mouse strains such as the BALB/c strain; the standard strain, C57BL/6J (B6J), does not develop ADR nephropathy. Nevertheless, Arif et al. reported that C57BL/6N (B6N), another standard strain, is ADR-susceptible. Since then, no follow-up reports or other studies have been published on ADR nephropathy in B6N mice. Therefore, the goal of this stud...
Source: Experimental Animals - June 21, 2023 Category: Research Authors: Masaki Watanabe Momoka Kakutani Koki Hiura Hayato Sasaki Nobuya Sasaki Source Type: research
