Panax quinquefolium saponins attenuates microglia activation following acute cerebral ischemia-reperfusion injury via Nrf2/miR-103-3p/TANK pathway
In conclusion, our study not only revealed the new mechanism of PQS in protecting against the inflammatory activation of microglia cells caused by cerebral ischemia-reperfusion injury, but also suggested that Nrf2 is a potential target for development of new drugs of ischemic stroke. More importantly, our study also reminded that miR-103-3p might be used as a prognostic biomarker for patients with ischemic stroke.PMID:37885132 | DOI:10.1002/cbin.12100 (Source: Cell Biology International)
Source: Cell Biology International - October 27, 2023 Category: Cytology Authors: Xuesong Bai Yan Qiu Jian Wang Yafen Dong Tao Zhang Hui Jin Source Type: research
Neosetophomone B induces apoptosis in multiple myeloma cells via targeting of AKT/SKP2 signaling pathway
Cell Biol Int. 2023 Oct 26. doi: 10.1002/cbin.12101. Online ahead of print.ABSTRACTMultiple myeloma (MM) is a hematologic malignancy associated with malignant plasma cell proliferation in the bone marrow. Despite the available treatments, drug resistance and adverse side effects pose significant challenges, underscoring the need for alternative therapeutic strategies. Natural products, like the fungal metabolite neosetophomone B (NSP-B), have emerged as potential therapeutic agents due to their bioactive properties. Our study investigated NSP-B's antitumor effects on MM cell lines (U266 and RPMI8226) and the involved molec...
Source: Cell Biology International - October 27, 2023 Category: Cytology Authors: Shilpa Kuttikrishnan Fareed Ahmad Jericha M Mateo Kirti S Prabhu Tamam El-Elimat Nicholas H Oberlies Cedric J Pearce Ammira S Alshabeeb Akil Ajaz A Bhat Feras Q Alali Shahab Uddin Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Multifaceted TGF- β signaling, a master regulator: From bench-to-bedside, intricacies, and complexities
Cell Biol Int. 2023 Oct 19. doi: 10.1002/cbin.12097. Online ahead of print.ABSTRACTPhysiological embryogenesis and adult tissue homeostasis are regulated by transforming growth factor-β (TGF-β), an evolutionarily conserved family of secreted polypeptide factors, acting in an autocrine and paracrine manner. The role of TGF-β in inflammation, fibrosis, and cancer is complex and sometimes even contradictory, exhibiting either inhibitory or promoting effects depending on the stage of the disease. Under pathological conditions, especially fibrosis and cancer, overexpressed TGF-β causes extracellular matrix deposition, epith...
Source: Cell Biology International - October 20, 2023 Category: Cytology Authors: Sana Ahuja Sufian Zaheer Source Type: research
Silencing geranylgeranyltransferase I inhibits the migration and invasion of salivary adenoid cystic carcinoma through RhoA/ROCK1/MLC signaling and suppresses proliferation through cell cycle regulation
This study primarily aimed to investigate the role and mechanism of GGTase-I in mediating the malignant progression of SACC. The level of GGTase-I gene in cells was stably knocked down by short hairpin RNA-EGFP-lentivirus. The effects of GGTase-I silencing on the migration, invasion, and spread of cells were examined, the messenger RNA levels of GGTase-I and RhoA genes of SACC cells after GGTase-I knockdown were determined, and the protein levels of RhoA and RhoA membrane of SACC cells were analyzed. Moreover, the potential underlying mechanism of silencing GGTase-I on the above-mentioned aspects in SACC cells was assessed...
Source: Cell Biology International - October 19, 2023 Category: Cytology Authors: Zengpeng Chi Qimin Wang Lei Tong Jing Qiu Fang Yang Qingyuan Guo Wenjian Li Jiawei Zheng Zhenggang Chen Source Type: research
Silencing geranylgeranyltransferase I inhibits the migration and invasion of salivary adenoid cystic carcinoma through RhoA/ROCK1/MLC signaling and suppresses proliferation through cell cycle regulation
This study primarily aimed to investigate the role and mechanism of GGTase-I in mediating the malignant progression of SACC. The level of GGTase-I gene in cells was stably knocked down by short hairpin RNA-EGFP-lentivirus. The effects of GGTase-I silencing on the migration, invasion, and spread of cells were examined, the messenger RNA levels of GGTase-I and RhoA genes of SACC cells after GGTase-I knockdown were determined, and the protein levels of RhoA and RhoA membrane of SACC cells were analyzed. Moreover, the potential underlying mechanism of silencing GGTase-I on the above-mentioned aspects in SACC cells was assessed...
Source: Cell Biology International - October 19, 2023 Category: Cytology Authors: Zengpeng Chi Qimin Wang Lei Tong Jing Qiu Fang Yang Qingyuan Guo Wenjian Li Jiawei Zheng Zhenggang Chen Source Type: research
Methods used to achieve different levels of the neuronal differentiation process in SH-SY5Y and Neuro2a cell lines: An integrative review
Cell Biol Int. 2023 Oct 10. doi: 10.1002/cbin.12093. Online ahead of print.ABSTRACTTo study the process of neuronal differentiation, the human neuroblastoma (SH-SY5Y) and the murine neuroblastoma (Neuro2a) cell lines have proven to be effective models. For this approach, different protocols involving known neurotrophic factors and other molecules, such as retinoic acid (RA), have been assessed to better understand the neuronal differentiation process. Thus, the goal of this manuscript was to provide a brief overview of recent studies that have used protocols to promote neurodifferentiation in SH-SY5Y and Neuro2a cell lines...
Source: Cell Biology International - October 11, 2023 Category: Cytology Authors: M ônica G Dos Santos Jos é R Gomes Michele D M Costa Source Type: research
Elevated extracellular inorganic phosphate inhibits ecto-phosphatase activity in breast cancer cells: Regulation by hydrogen peroxide
In this study, we show that increased Pi levels in the extracellular environment promote a decrease in ecto-phosphatase activity, which is associated with Pi-induced oxidative stress. High levels of Pi inhibit ecto-phosphatase because Pi generates H2 O2 . Ecto-phosphatase activity is inhibited by H2 O2 , and this inhibition is selective for phospho-tyrosine hydrolysis. Additionally, it is shown that the mechanism of inhibition of ecto-phosphatase activity involves lipid peroxidation. In addition, the inhibition of ecto-phosphatase activity by H2 O2 is irreversible. These findings have new implications for understanding ect...
Source: Cell Biology International - October 11, 2023 Category: Cytology Authors: Marco A Lacerda-Abreu Jos é R Meyer-Fernandes Source Type: research
Methods used to achieve different levels of the neuronal differentiation process in SH-SY5Y and Neuro2a cell lines: An integrative review
Cell Biol Int. 2023 Oct 10. doi: 10.1002/cbin.12093. Online ahead of print.ABSTRACTTo study the process of neuronal differentiation, the human neuroblastoma (SH-SY5Y) and the murine neuroblastoma (Neuro2a) cell lines have proven to be effective models. For this approach, different protocols involving known neurotrophic factors and other molecules, such as retinoic acid (RA), have been assessed to better understand the neuronal differentiation process. Thus, the goal of this manuscript was to provide a brief overview of recent studies that have used protocols to promote neurodifferentiation in SH-SY5Y and Neuro2a cell lines...
Source: Cell Biology International - October 11, 2023 Category: Cytology Authors: M ônica G Dos Santos Jos é R Gomes Michele D M Costa Source Type: research
Elevated extracellular inorganic phosphate inhibits ecto-phosphatase activity in breast cancer cells: Regulation by hydrogen peroxide
In this study, we show that increased Pi levels in the extracellular environment promote a decrease in ecto-phosphatase activity, which is associated with Pi-induced oxidative stress. High levels of Pi inhibit ecto-phosphatase because Pi generates H2 O2 . Ecto-phosphatase activity is inhibited by H2 O2 , and this inhibition is selective for phospho-tyrosine hydrolysis. Additionally, it is shown that the mechanism of inhibition of ecto-phosphatase activity involves lipid peroxidation. In addition, the inhibition of ecto-phosphatase activity by H2 O2 is irreversible. These findings have new implications for understanding ect...
Source: Cell Biology International - October 11, 2023 Category: Cytology Authors: Marco A Lacerda-Abreu Jos é R Meyer-Fernandes Source Type: research
Methods used to achieve different levels of the neuronal differentiation process in SH-SY5Y and Neuro2a cell lines: An integrative review
Cell Biol Int. 2023 Oct 10. doi: 10.1002/cbin.12093. Online ahead of print.ABSTRACTTo study the process of neuronal differentiation, the human neuroblastoma (SH-SY5Y) and the murine neuroblastoma (Neuro2a) cell lines have proven to be effective models. For this approach, different protocols involving known neurotrophic factors and other molecules, such as retinoic acid (RA), have been assessed to better understand the neuronal differentiation process. Thus, the goal of this manuscript was to provide a brief overview of recent studies that have used protocols to promote neurodifferentiation in SH-SY5Y and Neuro2a cell lines...
Source: Cell Biology International - October 11, 2023 Category: Cytology Authors: M ônica G Dos Santos Jos é R Gomes Michele D M Costa Source Type: research
