HMGB1 in exosomes derived from gastric cancer cells induces M2-like macrophage polarization by inhibiting the NF- κB signaling pathway
This study mainly determined the regulatory effect of HMGB1 in GC cell-derived exosomes on M2-like macrophage polarization as well as the underlying mechanism. HMGB1 was found to be highly expressed in gastric tissue specimens, which might lead to the poor prognosis of GC. High levels of HMGB1 were also observed in the plasma of GC patients, indicating the possibility that it regulates the immune microenvironment via exosomes. Further study revealed and confirmed the regulatory effect of exosomes derived from GC cells with high HMGB1 level on inducing M2-like macrophage polarization. Mechanistically, by interacting with th...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Ke Liu Hui Wang Jumei Zhou Suyu Zhu Min Ma Hua Xiao Yi Ding Source Type: research
Deoxyribonuclease 1-like 3 inhibits colorectal malignancy through antagonizing NEDD4-triggered CDKN1A ubiquitination
Cell Biol Int. 2023 Dec 18. doi: 10.1002/cbin.12108. Online ahead of print.ABSTRACTDeoxyribonuclease 1-like 3 (DNASE1L3) has been shown to play nonnegligible roles in several types of carcinomas. Nevertheless, the biological function, clinical relevance, and influence of DNASE1L3 in colorectal cancer (CRC) remain obscure. Immunohistochemistry was adopted to examine DNASE1L3 and CDKN1A expression in CRC tissue, and the clinical significance of DNASE1L3 was assessed. Cell counting kit-8, colony formation, and transwell assays were employed for assessing tumor proliferation and migration. The mechanisms underlying the impact ...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Lifei Yu Jin Huang Source Type: research
HMGB1 in exosomes derived from gastric cancer cells induces M2-like macrophage polarization by inhibiting the NF- κB signaling pathway
This study mainly determined the regulatory effect of HMGB1 in GC cell-derived exosomes on M2-like macrophage polarization as well as the underlying mechanism. HMGB1 was found to be highly expressed in gastric tissue specimens, which might lead to the poor prognosis of GC. High levels of HMGB1 were also observed in the plasma of GC patients, indicating the possibility that it regulates the immune microenvironment via exosomes. Further study revealed and confirmed the regulatory effect of exosomes derived from GC cells with high HMGB1 level on inducing M2-like macrophage polarization. Mechanistically, by interacting with th...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Ke Liu Hui Wang Jumei Zhou Suyu Zhu Min Ma Hua Xiao Yi Ding Source Type: research
Deoxyribonuclease 1-like 3 inhibits colorectal malignancy through antagonizing NEDD4-triggered CDKN1A ubiquitination
Cell Biol Int. 2023 Dec 18. doi: 10.1002/cbin.12108. Online ahead of print.ABSTRACTDeoxyribonuclease 1-like 3 (DNASE1L3) has been shown to play nonnegligible roles in several types of carcinomas. Nevertheless, the biological function, clinical relevance, and influence of DNASE1L3 in colorectal cancer (CRC) remain obscure. Immunohistochemistry was adopted to examine DNASE1L3 and CDKN1A expression in CRC tissue, and the clinical significance of DNASE1L3 was assessed. Cell counting kit-8, colony formation, and transwell assays were employed for assessing tumor proliferation and migration. The mechanisms underlying the impact ...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Lifei Yu Jin Huang Source Type: research
HMGB1 in exosomes derived from gastric cancer cells induces M2-like macrophage polarization by inhibiting the NF- κB signaling pathway
This study mainly determined the regulatory effect of HMGB1 in GC cell-derived exosomes on M2-like macrophage polarization as well as the underlying mechanism. HMGB1 was found to be highly expressed in gastric tissue specimens, which might lead to the poor prognosis of GC. High levels of HMGB1 were also observed in the plasma of GC patients, indicating the possibility that it regulates the immune microenvironment via exosomes. Further study revealed and confirmed the regulatory effect of exosomes derived from GC cells with high HMGB1 level on inducing M2-like macrophage polarization. Mechanistically, by interacting with th...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Ke Liu Hui Wang Jumei Zhou Suyu Zhu Min Ma Hua Xiao Yi Ding Source Type: research
Deoxyribonuclease 1-like 3 inhibits colorectal malignancy through antagonizing NEDD4-triggered CDKN1A ubiquitination
Cell Biol Int. 2023 Dec 18. doi: 10.1002/cbin.12108. Online ahead of print.ABSTRACTDeoxyribonuclease 1-like 3 (DNASE1L3) has been shown to play nonnegligible roles in several types of carcinomas. Nevertheless, the biological function, clinical relevance, and influence of DNASE1L3 in colorectal cancer (CRC) remain obscure. Immunohistochemistry was adopted to examine DNASE1L3 and CDKN1A expression in CRC tissue, and the clinical significance of DNASE1L3 was assessed. Cell counting kit-8, colony formation, and transwell assays were employed for assessing tumor proliferation and migration. The mechanisms underlying the impact ...
Source: Cell Biology International - December 18, 2023 Category: Cytology Authors: Lifei Yu Jin Huang Source Type: research
14-3-3- η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage
In this study, we observed that the expression of 14-3-3-η and BCL-2 were downregulated in ox-LDL-treated EPCs. Overexpression of 14-3-3-η in ox-LDL-treated EPC significantly increased BCL-2 level, while knockdown of BCL-2 reduced 14-3-3-η expression and mitigated the protective effect of 14-3-3-η on EPCs. In addition, we discovered that 14-3-3-η colocalizes and interacts with BCL-2 in EPCs. Taken together, these data suggest that 14-3-3-η protects EPCs from ox-LDL-induced damage by its interaction with BCL-2.PMID:38100125 | DOI:10.1002/cbin.12105 (Source: Cell Biology International)
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Hui Zhou Xiaopei Sun Yi Dai Xiaotong Wang Zhihong Dai Xiuli Li Source Type: research
dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using Staphylococcus aureus
In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status of the AKT1 promoter in BMMECs. We found that the degree of the AKT1 promoter methylation in BMMECs was significantly greater than that in BMECs, and the expression levels of genes related to milk protein synthesis were significantly decreased. We used the pdCas9-C-Tet1-SgRNA 2.0 system to regulate the methylation status of the AKT1 promoter. High-efficiency sgRNAs were screened and...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Jie Liu Xiangfei Wei Yan Zhang Yaoxiang Ran Bo Qu Chunmei Wang Feng Zhao Li Zhang Source Type: research
Extracellular vesicles from oral mucosa stem cells promote lipid raft formation in human microglia through TLR4, P2X4R, and αVβ3/αVβ5 signaling pathways
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12111. Online ahead of print.ABSTRACTTargeting of disease-associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, the use of extracellular vesicles (EVs) represents a promising therapeutic approach. However, the molecular mechanisms by which EVs regulate microglial responses remain poorly understood. In the present study, we used EVs derived from human oral mucosa stem cells (OMSCs) to investigate the effects on the lipid raft formation and the phagocytic response of human microglial...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Diana Romenskaja Ugn ė Jonavičė Virginijus Tunaitis Augustas Pivori ūnas Source Type: research
The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12107. Online ahead of print.ABSTRACTThe therapeutic potential of stem cells-derived extracellular vesicles (EVs) has shown a great progress in the regenerative medicine. EVs are rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived EVs regulate immunity, promote cell proliferation and differentiation, enhance bone and vascular regeneration, and play an increasingly importa...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Zhuo Li Demiao Hou Zijin Tang Lishun Xiong Yiguo Yan Source Type: research
14-3-3- η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage
In this study, we observed that the expression of 14-3-3-η and BCL-2 were downregulated in ox-LDL-treated EPCs. Overexpression of 14-3-3-η in ox-LDL-treated EPC significantly increased BCL-2 level, while knockdown of BCL-2 reduced 14-3-3-η expression and mitigated the protective effect of 14-3-3-η on EPCs. In addition, we discovered that 14-3-3-η colocalizes and interacts with BCL-2 in EPCs. Taken together, these data suggest that 14-3-3-η protects EPCs from ox-LDL-induced damage by its interaction with BCL-2.PMID:38100125 | DOI:10.1002/cbin.12105 (Source: Cell Biology International)
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Hui Zhou Xiaopei Sun Yi Dai Xiaotong Wang Zhihong Dai Xiuli Li Source Type: research
dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using Staphylococcus aureus
In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status of the AKT1 promoter in BMMECs. We found that the degree of the AKT1 promoter methylation in BMMECs was significantly greater than that in BMECs, and the expression levels of genes related to milk protein synthesis were significantly decreased. We used the pdCas9-C-Tet1-SgRNA 2.0 system to regulate the methylation status of the AKT1 promoter. High-efficiency sgRNAs were screened and...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Jie Liu Xiangfei Wei Yan Zhang Yaoxiang Ran Bo Qu Chunmei Wang Feng Zhao Li Zhang Source Type: research
Extracellular vesicles from oral mucosa stem cells promote lipid raft formation in human microglia through TLR4, P2X4R, and αVβ3/αVβ5 signaling pathways
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12111. Online ahead of print.ABSTRACTTargeting of disease-associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, the use of extracellular vesicles (EVs) represents a promising therapeutic approach. However, the molecular mechanisms by which EVs regulate microglial responses remain poorly understood. In the present study, we used EVs derived from human oral mucosa stem cells (OMSCs) to investigate the effects on the lipid raft formation and the phagocytic response of human microglial...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Diana Romenskaja Ugn ė Jonavičė Virginijus Tunaitis Augustas Pivori ūnas Source Type: research
The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12107. Online ahead of print.ABSTRACTThe therapeutic potential of stem cells-derived extracellular vesicles (EVs) has shown a great progress in the regenerative medicine. EVs are rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived EVs regulate immunity, promote cell proliferation and differentiation, enhance bone and vascular regeneration, and play an increasingly importa...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Zhuo Li Demiao Hou Zijin Tang Lishun Xiong Yiguo Yan Source Type: research
14-3-3- η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage
In this study, we observed that the expression of 14-3-3-η and BCL-2 were downregulated in ox-LDL-treated EPCs. Overexpression of 14-3-3-η in ox-LDL-treated EPC significantly increased BCL-2 level, while knockdown of BCL-2 reduced 14-3-3-η expression and mitigated the protective effect of 14-3-3-η on EPCs. In addition, we discovered that 14-3-3-η colocalizes and interacts with BCL-2 in EPCs. Taken together, these data suggest that 14-3-3-η protects EPCs from ox-LDL-induced damage by its interaction with BCL-2.PMID:38100125 | DOI:10.1002/cbin.12105 (Source: Cell Biology International)
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Hui Zhou Xiaopei Sun Yi Dai Xiaotong Wang Zhihong Dai Xiuli Li Source Type: research
