Dihydroartemisinin eliminates senescent cells by promoting autophagy-dependent ferroptosis via AMPK/mTOR signaling pathway
Cell Biol Int. 2024 Mar 4. doi: 10.1002/cbin.12143. Online ahead of print.ABSTRACTCellular senescence is an irreversible cell-cycle arrest in response to a variety of cellular stresses, which contribute to the pathogenesis of a variety of age-related degenerative diseases. However, effective antisenescence strategies are still lacking. Drugs that selectively target senescent cells represent an intriguing therapeutic strategy to delay aging and age-related diseases. Thus, we thought to investigate the effects of dihydroartemisinin (DHA) on senescent cells and elucidated its mechanisms underlying aging. Stress-induced premat...
Source: Cell Biology International - March 5, 2024 Category: Cytology Authors: Xing Wan Can Li Yue Hao Tan Shi Qi Zuo Feng Mei Deng Jing Sun Yi Lun Liu Source Type: research
Identification and validation of m6A-associated ferroptosis genes in renal clear cell carcinoma
We examined patients' immune profiles by risk score. Our prognostic genes predicted ccRCC treatment drugs. We found 37 DEMFRGs by comparing 1913 differentially expressed ccRCC genes to 202 m6A RNA methylation FRGs. Functional enrichment analysis showed that hypoxia-induced cell death and metabolism pathways were the most differentially expressed methylation functional regulating genes. Five prognostic genes were found by machine learning: TRIB3, CHAC1, NNMT, EGFR, and SLC1A4. An advanced renal cell carcinoma nomogram with age and risk score accurately predicted the outcome. These five prognostic genes were linked to variou...
Source: Cell Biology International - March 5, 2024 Category: Cytology Authors: Shuo Pang Shuo Zhao Yuxi Dongye Yidong Fan Jikai Liu Source Type: research
Dihydroartemisinin eliminates senescent cells by promoting autophagy-dependent ferroptosis via AMPK/mTOR signaling pathway
Cell Biol Int. 2024 Mar 4. doi: 10.1002/cbin.12143. Online ahead of print.ABSTRACTCellular senescence is an irreversible cell-cycle arrest in response to a variety of cellular stresses, which contribute to the pathogenesis of a variety of age-related degenerative diseases. However, effective antisenescence strategies are still lacking. Drugs that selectively target senescent cells represent an intriguing therapeutic strategy to delay aging and age-related diseases. Thus, we thought to investigate the effects of dihydroartemisinin (DHA) on senescent cells and elucidated its mechanisms underlying aging. Stress-induced premat...
Source: Cell Biology International - March 5, 2024 Category: Cytology Authors: Xing Wan Can Li Yue Hao Tan Shi Qi Zuo Feng Mei Deng Jing Sun Yi Lun Liu Source Type: research
Identification and validation of m6A-associated ferroptosis genes in renal clear cell carcinoma
We examined patients' immune profiles by risk score. Our prognostic genes predicted ccRCC treatment drugs. We found 37 DEMFRGs by comparing 1913 differentially expressed ccRCC genes to 202 m6A RNA methylation FRGs. Functional enrichment analysis showed that hypoxia-induced cell death and metabolism pathways were the most differentially expressed methylation functional regulating genes. Five prognostic genes were found by machine learning: TRIB3, CHAC1, NNMT, EGFR, and SLC1A4. An advanced renal cell carcinoma nomogram with age and risk score accurately predicted the outcome. These five prognostic genes were linked to variou...
Source: Cell Biology International - March 5, 2024 Category: Cytology Authors: Shuo Pang Shuo Zhao Yuxi Dongye Yidong Fan Jikai Liu Source Type: research
CoCl < sub > 2 < /sub > -Induced hypoxia promotes hPDLSCs osteogenic differentiation through AKT/mTOR/4EBP-1/HIF-1 α signaling and facilitates the repair of alveolar bone defects
This study highlighted the importance of physiological oxygen conditions for tissue engineering.PMID:38433534 | DOI:10.1002/cbin.12148 (Source: Cell Biology International)
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Zhibo Fan Yanru Liu Yuxin Lan Yujie Wu Junyu Li Xiaomei Xu Source Type: research
Follistatin-like 1 protects against doxorubicin-induced cardiotoxicity by preventing mitochondrial dysfunction through the SIRT6/Nrf2 signaling pathway
Cell Biol Int. 2024 Mar 4. doi: 10.1002/cbin.12147. Online ahead of print.ABSTRACTMitochondrial dysfunction and myocardial remodeling have been reported to be the main underlying molecular mechanisms of doxorubicin-induced cardiotoxicity. SIRT6 is a nicotinamide adenine dinucleotide-dependent enzyme that plays a vital role in cardiac protection against various stresses. Moreover, previous studies have demonstrated that FSTL1 could alleviate doxorubicin-induced cardiotoxicity by inhibiting autophagy. The present study investigated the probable mechanisms of FSTL1 on doxorubicin-induced cardiotoxicity in vivo and in vitro. W...
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Haijun Xu Hong Guo Zhigang Tang Ruijun Hao Shaowei Wang Ping Jin Source Type: research
Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes
In this study, we treated immortalized human keratinocytes (HaCaT cells) with low-dose ALA-PDT (0.5 mmol/L, 3 J/cm2 ). Through RNA-sequencing analysis, we identified that low-dose ALA-PDT modulated autophagy-related pathways in keratinocytes and pinpointed Unc-51-like kinase 1 (ULK1) as a key gene involved. Western blot results revealed that low-dose ALA-PDT treatment upregulated the expression of autophagy-related proteins Beclin-1 and LC3-II/LC3-I ratio. Notably, low-dose ALA-PDT regulated autophagy by inducing an appropriate level of reactive oxygen species (ROS), transiently reducing mitochondrial membrane potential, a...
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Zhinan Shi Jing Wang Min Li Li Gu Zhiyi Xu Xiaoyu Zhai Shu Zhou Jingting Zhao Liqun Gu Lin Chen Linling Ju Bingrong Zhou Hui Hua Source Type: research
CoCl < sub > 2 < /sub > -Induced hypoxia promotes hPDLSCs osteogenic differentiation through AKT/mTOR/4EBP-1/HIF-1 α signaling and facilitates the repair of alveolar bone defects
This study highlighted the importance of physiological oxygen conditions for tissue engineering.PMID:38433534 | DOI:10.1002/cbin.12148 (Source: Cell Biology International)
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Zhibo Fan Yanru Liu Yuxin Lan Yujie Wu Junyu Li Xiaomei Xu Source Type: research
Follistatin-like 1 protects against doxorubicin-induced cardiotoxicity by preventing mitochondrial dysfunction through the SIRT6/Nrf2 signaling pathway
Cell Biol Int. 2024 Mar 4. doi: 10.1002/cbin.12147. Online ahead of print.ABSTRACTMitochondrial dysfunction and myocardial remodeling have been reported to be the main underlying molecular mechanisms of doxorubicin-induced cardiotoxicity. SIRT6 is a nicotinamide adenine dinucleotide-dependent enzyme that plays a vital role in cardiac protection against various stresses. Moreover, previous studies have demonstrated that FSTL1 could alleviate doxorubicin-induced cardiotoxicity by inhibiting autophagy. The present study investigated the probable mechanisms of FSTL1 on doxorubicin-induced cardiotoxicity in vivo and in vitro. W...
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Haijun Xu Hong Guo Zhigang Tang Ruijun Hao Shaowei Wang Ping Jin Source Type: research
Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes
In this study, we treated immortalized human keratinocytes (HaCaT cells) with low-dose ALA-PDT (0.5 mmol/L, 3 J/cm2 ). Through RNA-sequencing analysis, we identified that low-dose ALA-PDT modulated autophagy-related pathways in keratinocytes and pinpointed Unc-51-like kinase 1 (ULK1) as a key gene involved. Western blot results revealed that low-dose ALA-PDT treatment upregulated the expression of autophagy-related proteins Beclin-1 and LC3-II/LC3-I ratio. Notably, low-dose ALA-PDT regulated autophagy by inducing an appropriate level of reactive oxygen species (ROS), transiently reducing mitochondrial membrane potential, a...
Source: Cell Biology International - March 4, 2024 Category: Cytology Authors: Zhinan Shi Jing Wang Min Li Li Gu Zhiyi Xu Xiaoyu Zhai Shu Zhou Jingting Zhao Liqun Gu Lin Chen Linling Ju Bingrong Zhou Hui Hua Source Type: research
Assessment of human embryonic stem cells differentiation into definitive endoderm lineage on the soft substrates
Cell Biol Int. 2024 Feb 29. doi: 10.1002/cbin.12151. Online ahead of print.ABSTRACTPluripotent stem cells (PSCs) hold enormous potential for treating multiple diseases owing to their ability to self-renew and differentiate into any cell type. Albeit possessing such promising potential, controlling their differentiation into a desired cell type continues to be a challenge. Recent studies suggest that PSCs respond to different substrate stiffness and, therefore, can differentiate towards some lineages via Hippo pathway. Human PSCs can also differentiate and self-organize into functional cells, such as organoids. Traditionall...
Source: Cell Biology International - February 29, 2024 Category: Cytology Authors: Jasmeet Kaur Virdi Prasad Pethe Source Type: research
Epigenetic changes in shear-stressed endothelial cells
Cell Biol Int. 2024 Feb 29. doi: 10.1002/cbin.12138. Online ahead of print.ABSTRACTEpigenetic changes, particularly histone compaction modifications, have emerged as critical regulators in the epigenetic pathway driving endothelial cell phenotype under constant exposure to laminar forces induced by blood flow. However, the underlying epigenetic mechanisms governing endothelial cell behavior in this context remain poorly understood. To address this knowledge gap, we conducted in vitro experiments using human umbilical vein endothelial cells subjected to various tensional forces simulating pathophysiological blood flow shear...
Source: Cell Biology International - February 29, 2024 Category: Cytology Authors: Tha ís Silva Pinto Ge órgia da Silva Feltran C élio Júnior da C Fernandes Amanda Fantini de Camargo Andrade Alex de Camargo Coque Simone L Silva Abdulwahab A Abuderman Willian F Zambuzzi Rodrigo A Foganholi da Silva Source Type: research
The ultrastructure of the apical organ of Curini-Galletti's larva, a new polyclad larval type
Cell Biol Int. 2024 Feb 29. doi: 10.1002/cbin.12139. Online ahead of print.ABSTRACTPolycladida are the only free-living flatworms with a planktonic larval stage in some species. Currently, it is not clear if a larval stage is ancestral in polyclads, and which type of larva that would be. Known polyclad larvae are Müller's larva, Kato's larva and Goette's larva, differing by body shape and the number of lobes and eyes. A valuable character for the comparison and characterisation of polyclad larval types is the ultrastructural composition of the apical organ. This organ is situated at the anterior pole of the larva and is a...
Source: Cell Biology International - February 29, 2024 Category: Cytology Authors: Isabel L Dittmann Philip Bertemes Clemens Gotsis Alexandra L Grosbusch Stefan Redl Michael W Hess Willi Salvenmoser Bernhard Egger Source Type: research
Assessment of human embryonic stem cells differentiation into definitive endoderm lineage on the soft substrates
Cell Biol Int. 2024 Feb 29. doi: 10.1002/cbin.12151. Online ahead of print.ABSTRACTPluripotent stem cells (PSCs) hold enormous potential for treating multiple diseases owing to their ability to self-renew and differentiate into any cell type. Albeit possessing such promising potential, controlling their differentiation into a desired cell type continues to be a challenge. Recent studies suggest that PSCs respond to different substrate stiffness and, therefore, can differentiate towards some lineages via Hippo pathway. Human PSCs can also differentiate and self-organize into functional cells, such as organoids. Traditionall...
Source: Cell Biology International - February 29, 2024 Category: Cytology Authors: Jasmeet Kaur Virdi Prasad Pethe Source Type: research
Epigenetic changes in shear-stressed endothelial cells
Cell Biol Int. 2024 Feb 29. doi: 10.1002/cbin.12138. Online ahead of print.ABSTRACTEpigenetic changes, particularly histone compaction modifications, have emerged as critical regulators in the epigenetic pathway driving endothelial cell phenotype under constant exposure to laminar forces induced by blood flow. However, the underlying epigenetic mechanisms governing endothelial cell behavior in this context remain poorly understood. To address this knowledge gap, we conducted in vitro experiments using human umbilical vein endothelial cells subjected to various tensional forces simulating pathophysiological blood flow shear...
Source: Cell Biology International - February 29, 2024 Category: Cytology Authors: Tha ís Silva Pinto Ge órgia da Silva Feltran C élio Júnior da C Fernandes Amanda Fantini de Camargo Andrade Alex de Camargo Coque Simone L Silva Abdulwahab A Abuderman Willian F Zambuzzi Rodrigo A Foganholi da Silva Source Type: research
