dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using Staphylococcus aureus
In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status of the AKT1 promoter in BMMECs. We found that the degree of the AKT1 promoter methylation in BMMECs was significantly greater than that in BMECs, and the expression levels of genes related to milk protein synthesis were significantly decreased. We used the pdCas9-C-Tet1-SgRNA 2.0 system to regulate the methylation status of the AKT1 promoter. High-efficiency sgRNAs were screened and...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Jie Liu Xiangfei Wei Yan Zhang Yaoxiang Ran Bo Qu Chunmei Wang Feng Zhao Li Zhang Source Type: research
Extracellular vesicles from oral mucosa stem cells promote lipid raft formation in human microglia through TLR4, P2X4R, and αVβ3/αVβ5 signaling pathways
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12111. Online ahead of print.ABSTRACTTargeting of disease-associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, the use of extracellular vesicles (EVs) represents a promising therapeutic approach. However, the molecular mechanisms by which EVs regulate microglial responses remain poorly understood. In the present study, we used EVs derived from human oral mucosa stem cells (OMSCs) to investigate the effects on the lipid raft formation and the phagocytic response of human microglial...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Diana Romenskaja Ugn ė Jonavičė Virginijus Tunaitis Augustas Pivori ūnas Source Type: research
The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12107. Online ahead of print.ABSTRACTThe therapeutic potential of stem cells-derived extracellular vesicles (EVs) has shown a great progress in the regenerative medicine. EVs are rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived EVs regulate immunity, promote cell proliferation and differentiation, enhance bone and vascular regeneration, and play an increasingly importa...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Zhuo Li Demiao Hou Zijin Tang Lishun Xiong Yiguo Yan Source Type: research
14-3-3- η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage
In this study, we observed that the expression of 14-3-3-η and BCL-2 were downregulated in ox-LDL-treated EPCs. Overexpression of 14-3-3-η in ox-LDL-treated EPC significantly increased BCL-2 level, while knockdown of BCL-2 reduced 14-3-3-η expression and mitigated the protective effect of 14-3-3-η on EPCs. In addition, we discovered that 14-3-3-η colocalizes and interacts with BCL-2 in EPCs. Taken together, these data suggest that 14-3-3-η protects EPCs from ox-LDL-induced damage by its interaction with BCL-2.PMID:38100125 | DOI:10.1002/cbin.12105 (Source: Cell Biology International)
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Hui Zhou Xiaopei Sun Yi Dai Xiaotong Wang Zhihong Dai Xiuli Li Source Type: research
dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using Staphylococcus aureus
In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status of the AKT1 promoter in BMMECs. We found that the degree of the AKT1 promoter methylation in BMMECs was significantly greater than that in BMECs, and the expression levels of genes related to milk protein synthesis were significantly decreased. We used the pdCas9-C-Tet1-SgRNA 2.0 system to regulate the methylation status of the AKT1 promoter. High-efficiency sgRNAs were screened and...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Jie Liu Xiangfei Wei Yan Zhang Yaoxiang Ran Bo Qu Chunmei Wang Feng Zhao Li Zhang Source Type: research
Extracellular vesicles from oral mucosa stem cells promote lipid raft formation in human microglia through TLR4, P2X4R, and αVβ3/αVβ5 signaling pathways
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12111. Online ahead of print.ABSTRACTTargeting of disease-associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, the use of extracellular vesicles (EVs) represents a promising therapeutic approach. However, the molecular mechanisms by which EVs regulate microglial responses remain poorly understood. In the present study, we used EVs derived from human oral mucosa stem cells (OMSCs) to investigate the effects on the lipid raft formation and the phagocytic response of human microglial...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Diana Romenskaja Ugn ė Jonavičė Virginijus Tunaitis Augustas Pivori ūnas Source Type: research
The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases
Cell Biol Int. 2023 Dec 15. doi: 10.1002/cbin.12107. Online ahead of print.ABSTRACTThe therapeutic potential of stem cells-derived extracellular vesicles (EVs) has shown a great progress in the regenerative medicine. EVs are rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived EVs regulate immunity, promote cell proliferation and differentiation, enhance bone and vascular regeneration, and play an increasingly importa...
Source: Cell Biology International - December 15, 2023 Category: Cytology Authors: Zhuo Li Demiao Hou Zijin Tang Lishun Xiong Yiguo Yan Source Type: research
Correction to "Pan-cancer analysis of the oncogenic role of HNRNPR in human tumors"
Cell Biol Int. 2023 Dec 12. doi: 10.1002/cbin.12109. Online ahead of print.NO ABSTRACTPMID:38084795 | DOI:10.1002/cbin.12109 (Source: Cell Biology International)
Source: Cell Biology International - December 12, 2023 Category: Cytology Source Type: research
Correction to "Pan-cancer analysis of the oncogenic role of HNRNPR in human tumors"
Cell Biol Int. 2023 Dec 12. doi: 10.1002/cbin.12109. Online ahead of print.NO ABSTRACTPMID:38084795 | DOI:10.1002/cbin.12109 (Source: Cell Biology International)
Source: Cell Biology International - December 12, 2023 Category: Cytology Source Type: research
Correction to "Pan-cancer analysis of the oncogenic role of HNRNPR in human tumors"
Cell Biol Int. 2023 Dec 12. doi: 10.1002/cbin.12109. Online ahead of print.NO ABSTRACTPMID:38084795 | DOI:10.1002/cbin.12109 (Source: Cell Biology International)
Source: Cell Biology International - December 12, 2023 Category: Cytology Source Type: research
The role of thrombomodulin in modulating ITGB3 expression and its implications for triple-negative breast cancer progression
Cell Biol Int. 2023 Dec 11. doi: 10.1002/cbin.12104. Online ahead of print.ABSTRACTTriple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC) compared to other BC subtypes in clinical settings. Currently, there are no effective therapeutic strategies for TNBC treatment. Therefore, there is an urgent need to identify suitable biomarkers or therapeutic targets for TNBC patients. Thrombomodulin (TM) plays a role in cancer progression and metastasis in many different cancers. However, the role of TM in TNBC is not yet fully understood. First, silenced-TM in MDA-MB-231 cells caused an increase in ...
Source: Cell Biology International - December 11, 2023 Category: Cytology Authors: Yu-Jia Chang G M Shazzad Hossain Prince Po-Li Wei Uyanga Batzorig Chien-Yu Huang Chin-Sheng Hung Tung-Cheng Chang Source Type: research
Positioning determines function: Wandering PKM2 performs different roles in tumor cells
Cell Biol Int. 2024 Jan;48(1):20-30. doi: 10.1002/cbin.12103. Epub 2023 Nov 17.ABSTRACTShort for pyruvate kinase M2 subtype, PKM2 can be said of all-round player that is notoriously known for its metabolic involvement in glycolysis. Holding a dural role as a metabolic or non-metabolic (kinase) enzyme, PKM2 has drawn extensive attention over its biological roles implicated in tumor cells, including proliferation, migration, invasion, metabolism, and so on. wandering PKM2 can be transboundary both intracellularly and extracellularly. Specifically, PKM2 can be nuclear, cytoplasmic, mitochondrial, exosomal, or even circulate w...
Source: Cell Biology International - November 17, 2023 Category: Cytology Authors: Shuo He Yan Liang Yiyi Tan Qing Liu Tao Liu Xiaomei Lu Shutao Zheng Source Type: research
Positioning determines function: Wandering PKM2 performs different roles in tumor cells
Cell Biol Int. 2023 Nov 17. doi: 10.1002/cbin.12103. Online ahead of print.ABSTRACTShort for pyruvate kinase M2 subtype, PKM2 can be said of all-round player that is notoriously known for its metabolic involvement in glycolysis. Holding a dural role as a metabolic or non-metabolic (kinase) enzyme, PKM2 has drawn extensive attention over its biological roles implicated in tumor cells, including proliferation, migration, invasion, metabolism, and so on. wandering PKM2 can be transboundary both intracellularly and extracellularly. Specifically, PKM2 can be nuclear, cytoplasmic, mitochondrial, exosomal, or even circulate withi...
Source: Cell Biology International - November 17, 2023 Category: Cytology Authors: Shuo He Yan Liang Yiyi Tan Qing Liu Tao Liu Xiaomei Lu Shutao Zheng Source Type: research
Positioning determines function: Wandering PKM2 performs different roles in tumor cells
Cell Biol Int. 2023 Nov 17. doi: 10.1002/cbin.12103. Online ahead of print.ABSTRACTShort for pyruvate kinase M2 subtype, PKM2 can be said of all-round player that is notoriously known for its metabolic involvement in glycolysis. Holding a dural role as a metabolic or non-metabolic (kinase) enzyme, PKM2 has drawn extensive attention over its biological roles implicated in tumor cells, including proliferation, migration, invasion, metabolism, and so on. wandering PKM2 can be transboundary both intracellularly and extracellularly. Specifically, PKM2 can be nuclear, cytoplasmic, mitochondrial, exosomal, or even circulate withi...
Source: Cell Biology International - November 17, 2023 Category: Cytology Authors: Shuo He Yan Liang Yiyi Tan Qing Liu Tao Liu Xiaomei Lu Shutao Zheng Source Type: research
Positioning determines function: Wandering PKM2 performs different roles in tumor cells
Cell Biol Int. 2023 Nov 17. doi: 10.1002/cbin.12103. Online ahead of print.ABSTRACTShort for pyruvate kinase M2 subtype, PKM2 can be said of all-round player that is notoriously known for its metabolic involvement in glycolysis. Holding a dural role as a metabolic or non-metabolic (kinase) enzyme, PKM2 has drawn extensive attention over its biological roles implicated in tumor cells, including proliferation, migration, invasion, metabolism, and so on. wandering PKM2 can be transboundary both intracellularly and extracellularly. Specifically, PKM2 can be nuclear, cytoplasmic, mitochondrial, exosomal, or even circulate withi...
Source: Cell Biology International - November 17, 2023 Category: Cytology Authors: Shuo He Yan Liang Yiyi Tan Qing Liu Tao Liu Xiaomei Lu Shutao Zheng Source Type: research
