Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro
In conclusion, our study demonstrates, for the first time, the resistance role of TCs in IUA disease, shedding light on their potential involvement in endometrial repair through the modulation of stem cell function.PMID:38353345 | DOI:10.1002/cbin.12137 (Source: Cell Biology International)
Source: Cell Biology International - February 14, 2024 Category: Cytology Authors: Xiaojiao Wei Hui Li Tianquan Chen Xiaojun Yang Source Type: research
Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro
In conclusion, our study demonstrates, for the first time, the resistance role of TCs in IUA disease, shedding light on their potential involvement in endometrial repair through the modulation of stem cell function.PMID:38353345 | DOI:10.1002/cbin.12137 (Source: Cell Biology International)
Source: Cell Biology International - February 14, 2024 Category: Cytology Authors: Xiaojiao Wei Hui Li Tianquan Chen Xiaojun Yang Source Type: research
Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro
In conclusion, our study demonstrates, for the first time, the resistance role of TCs in IUA disease, shedding light on their potential involvement in endometrial repair through the modulation of stem cell function.PMID:38353345 | DOI:10.1002/cbin.12137 (Source: Cell Biology International)
Source: Cell Biology International - February 14, 2024 Category: Cytology Authors: Xiaojiao Wei Hui Li Tianquan Chen Xiaojun Yang Source Type: research
The noncanonical nucleotide binding site 1 of the bile salt export pump is optimized for proper function of the transporter
In this study, we mutated two deviant residues in the signature sequence to generate a double mutant (R1221G/E1223Q). Furthermore, a triple mutant (E502S/R1221G/E1223Q) was generated, in which the deviant residues of the signature sequence and Q-loop were mutated concurrently to canonical residues. The double and triple mutants showed 80% and 60%, respectively, of the activity of wild-type BSEP. As expected, an increasing number of mutations gradually impair transport as an intricate network of interactions within the ABC proteins ensures proper functioning.PMID:38328902 | DOI:10.1002/cbin.12136 (Source: Cell Biology International)
Source: Cell Biology International - February 8, 2024 Category: Cytology Authors: Imran Sohail Mahmood Ul Hassan Diethart Schmid Peter Chiba Source Type: research
3D bio scaffold support osteogenic differentiation of mesenchymal stem cells
In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration. Mesenchymal stem cells (MSCs) isolated from the umbilical cord were seeded with hydrogel for 28 days, and cell-hydrogel composites were cultured in basal and osteogenic media. Alizarin red staining, quantitative polymerase chain reaction, and immunofluorescent staining were used to confirm that the hydrogel was biocompatible and capable of inducing osteogenic differentiation in umbilical cord-derived MSCs. The findings demonstrate that human MSCs differentiated into an osteogenic li...
Source: Cell Biology International - February 7, 2024 Category: Cytology Authors: Faiza Ramzan Shumaila Khalid Sobia Ekram Asmat Salim Trivia Frazier Sumreen Begum Omair A Mohiuddin Irfan Khan Source Type: research
A novel PDIA3/FTO/USP20 positive feedback regulatory loop induces osteogenic differentiation of preosteoblast in osteoporosis
In this study, m6 A RNA methylation was detected by methylated RNA immunoprecipitation (MeRIP), while mRNA stability was identified by RNA decay assay. Besides, protein-protein interaction and protein phosphorylation were determined using co-immunoprecipitation (Co-IP). Herein, results revealed that PDIA3 promoted osteogenic differentiation of preosteoblast MC3T3-E1. Besides, PDIA3 mRNA methylation was suppressed by FTO alpha-ketoglutarate dependent dioxygenase (FTO) as RNA methylation reduced PDIA3 mRNA stability during osteogenic differentiation of MC3T3-E1 cells. Moreover, ubiquitin specific peptidase 20 (USP20) improve...
Source: Cell Biology International - February 7, 2024 Category: Cytology Authors: Fei Zhang Chen Liu Zhiyong Chen Chengyi Zhao Source Type: research
M6A methylation-regulated autophagy may be a new therapeutic target for intervertebral disc degeneration
This article first summarizes the effects of various stressors on the death and autophagy of nucleus pulposus cells. Then, it summarizes the regulatory mechanism of m6A methylation on autophagy-related genes under stress and the role of these autophagy genes in nucleus pulposus cells. Finally, it proposes that the methylation modification of autophagy-related genes regulated by m6A may become a new treatment approach for intervertebral disc degeneration, providing new insights and ideas for the clinical treatment of intervertebral disc degeneration.PMID:38317355 | DOI:10.1002/cbin.12135 (Source: Cell Biology International)
Source: Cell Biology International - February 6, 2024 Category: Cytology Authors: Yuao Tao Xiaoyu Yu Xiaolong Li Yuzhu Xu Hui Wang Lele Zhang Rubing Lin Yuntao Wang Pan Fan Source Type: research
Endolysosomal two-pore channel 2 plays opposing roles in primary and metastatic malignant melanoma cells
Cell Biol Int. 2024 Jan 23. doi: 10.1002/cbin.12129. Online ahead of print.ABSTRACTThe ion channel two-pore channel 2 (TPC2), localised on the membranes of acidic organelles such as endo-lysosomes and melanosomes, has been shown to play a role in pathologies including cancer, and it is differently expressed in primary versus metastatic melanoma cells. Whether TPC2 plays a pro- or anti-oncogenic role in different tumour conditions is a relevant open question which we have explored in melanoma at different stages of tumour progression. The behaviour of primary melanoma cell line B16F0 and its metastatic subline B16F10 were c...
Source: Cell Biology International - January 24, 2024 Category: Cytology Authors: Samantha Barbonari Antonella D'Amore Ali A Hanbashi Fioretta Palombi Anna Riccioli John Parrington Antonio Filippini Source Type: research
Overexpression of Fut 2, 4, and 8, and nuclear localization of Fut 4 in ovarian cancer cell lines induced by ascitic fluids from epithelial ovarian cancer patients
Cell Biol Int. 2024 Jan 23. doi: 10.1002/cbin.12132. Online ahead of print.ABSTRACTFucosyltransferases (Fut) regulate the fucosylation process associated with tumorogenesis in different cancer types. Ascitic fluid (AF) from patients diagnosed with advanced stage of epithelial ovarian cancer (EOC) is considered as a dynamic tumor microenvironment associated with poor prognosis. Previous studies from our laboratory showed increased fucosylation in SKOV-3 and OVCAR-3, cancer-derived cell lines, when these cells were incubated with AFs derived from patients diagnosed with EOC. In the present work we studied three fucosyltransf...
Source: Cell Biology International - January 24, 2024 Category: Cytology Authors: Nayely Paulina Alvear-Hernandez Ver ónica Ivonne Hernández-Ramírez Julio C ésar Villegas-Pineda Juan Carlos Osorio-Trujillo Jos é Jesús Guzmán-Mendoza Dolores Gallardo-Rinc ón Alfredo Toledo-Leyva Patricia Talam ás-Rohana Source Type: research
What makes SARS-CoV-2 unique? Focusing on the spike protein
Cell Biol Int. 2024 Jan 23. doi: 10.1002/cbin.12130. Online ahead of print.ABSTRACTSevere acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) seriously threatens public health and safety. Genetic variants determine the expression of SARS-CoV-2 structural proteins, which are associated with enhanced transmissibility, enhanced virulence, and immune escape. Vaccination is encouraged as a public health intervention, and different types of vaccines are used worldwide. However, new variants continue to emerge, especially the Omicron complex, and the neutralizing antibody responses are diminished significantly. In this ...
Source: Cell Biology International - January 24, 2024 Category: Cytology Authors: Jingbo Qian Shichang Zhang Fang Wang Jinming Li Jiexin Zhang Source Type: research
LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling
This study confirmed that LRP8/p53 axis contributed to OC progression, which might serve as a novel potential therapeutic target for OC patients.PMID:38263609 | DOI:10.1002/cbin.12133 (Source: Cell Biology International)
Source: Cell Biology International - January 24, 2024 Category: Cytology Authors: Yan Xu Yang Zhou Xiling Yi Xiaocui Nie Source Type: research
A novel model for predicting prognosis in patients with idiopathic pulmonary fibrosis based on endoplasmic reticulum stress-related genes
In this study, we obtained transcriptome data and relevant clinical information from the Gene Expression Omnibus database and conducted bioinformatics analysis. Among the 544 ER stress-related genes (ERSRGs), 78 were identified as differentially expressed genes (DEGs). These DEGs were primarily enriched in response to ER stress, protein binding, and protein processing. Two genes (HTRA2 and KTN1) were included for constructing an accurate molecular signature. The overall survival of patients was remarkably worse in the high-risk group than in the low-risk group. We further analyzed the difference in immune cells between hig...
Source: Cell Biology International - January 19, 2024 Category: Cytology Authors: Bin Liu Xiang Zhang Zikai Liu Haihong Pan Hongxu Yang Qing Wu Yan Lv Tong Shen Source Type: research
THBS1 promotes angiogenesis and accelerates ESCC malignant progression by the HIF-1/VEGF signaling pathway
In this study, we future studied the specific role of THBS1 in ESCC and its molecular mechanism. Silencing THBS1 expression resulted in inhibition of cell migration and cell invasion of ESCC cells, the decrease of colony formation and proliferation. Tube formation of human umbilical vein endothelial cells (HUVECs) in vitro was decreased when cultured with conditioned medium from THBS1-silenced cells. The expression of CD31, a marker for blood vessel endothelial cells, was decreased in tumor tissues derived from THBS1-silenced tumors in vivo. Silencing THBS1 leaded the decreased of hypoxia-inducible factor-1α (HIF-1α), HI...
Source: Cell Biology International - January 18, 2024 Category: Cytology Authors: Xiao Zhou Qiaoxi Xia Mantong Chen Xiaona Zhang Meihui Huang Xiaoqi Zheng Shaohong Wang Bingli Wu Zepeng Du Source Type: research
The role of exo-miRNA in diagnosis and treatment of cancers, focusing on effective miRNAs in colorectal cancer
In conclusion, it is essential to continue research into exosome-based cancer medicines to advance understanding, improve treatment plans, create personalized tactics, ensure safety, and speed up clinical translation.PMID:38225535 | DOI:10.1002/cbin.12122 (Source: Cell Biology International)
Source: Cell Biology International - January 15, 2024 Category: Cytology Authors: Hamzah H Kzar Al-Shukri Sana Abdul-Jabbar Ali Karam Akram Al-Akkam Ahmed Hjazi Irodakhon Rasulova Yasser Fakri Mustafa Dahlia N Al-Saidi Mohammed Qasim Alasheqi Ahmed Alawadi Ali Alsaalamy Source Type: research
Mitochondrial RNA modification: A novel therapeutic target to combat metastasis
Cell Biol Int. 2024 Mar;48(3):233-236. doi: 10.1002/cbin.12124. Epub 2024 Jan 15.NO ABSTRACTPMID:38225665 | DOI:10.1002/cbin.12124 (Source: Cell Biology International)
Source: Cell Biology International - January 15, 2024 Category: Cytology Authors: Shifang Huang Honglu Jiang Zhen Huang Yuting Li Haoliang Hu Source Type: research
