Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Biomed Microdevices. 2023 Jun 22;25(3):23. doi: 10.1007/s10544-023-00663-1.ABSTRACTSickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testin...
Source: Biomedical Microdevices - June 22, 2023 Category: Biomedical Engineering Authors: Yuhao Qiang Darryl Dieujuste Jia Liu Ofelia Alvarez E Du Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
Development of a novel microfluidic perfusion 3D cell culture system for improved neuronal cell differentiation
Biomed Microdevices. 2023 Jun 13;25(3):22. doi: 10.1007/s10544-023-00660-4.ABSTRACTThree-dimensional (3D) cell cultures have recently gained popularity in the biomedical sciences because of their similarity to the in vivo environment. SH-SY5Y cells, which are neuronal cells and are commonly used to investigate neurodegenerative diseases, have particularly been reported to be differentiated as neuron-like cells expressing neuron-specific markers of mature neurons in static 3D culture environments when compared to static 2D environments, and those in perfusion environments have not yet been investigated. Microfluidic technol...
Source: Biomedical Microdevices - June 13, 2023 Category: Biomedical Engineering Authors: Dong Hyeok Park Mei Tong He Eun Ju Cho Karl Morten Jeung Sang Go Source Type: research
High-throughput microbead assay system with a portable, cost-effective Wi-Fi imaging module, and disposable multi-layered microfluidic cartridges for virus and microparticle detection, and tracking
We present the development of a wireless, standalone device and disposable microfluidics chips that rapidly generate parallel readouts for selected, possible virus variants from a nasal or saliva sample, based on motorized and non-motorized microbeads detection, and imaging processing of the motion tracks of these beads in micrometers. Microbeads and SARS-CoV-2 COVID-19 Delta variant were tested as proof-of-concept for testing the microfluidic cartridges and wireless imaging module. The Microbead Assay (MA) system kit consists of a Wi-Fi readout module, a microfluidic chip, and a sample collection/processing sub-system. He...
Source: Biomedical Microdevices - June 7, 2023 Category: Biomedical Engineering Authors: Jorge Manrique Castro Frank Sommerhage Rishika Khanna Andre Childs David DeRoo Swaminathan Rajaraman Source Type: research
High-throughput microbead assay system with a portable, cost-effective Wi-Fi imaging module, and disposable multi-layered microfluidic cartridges for virus and microparticle detection, and tracking
We present the development of a wireless, standalone device and disposable microfluidics chips that rapidly generate parallel readouts for selected, possible virus variants from a nasal or saliva sample, based on motorized and non-motorized microbeads detection, and imaging processing of the motion tracks of these beads in micrometers. Microbeads and SARS-CoV-2 COVID-19 Delta variant were tested as proof-of-concept for testing the microfluidic cartridges and wireless imaging module. The Microbead Assay (MA) system kit consists of a Wi-Fi readout module, a microfluidic chip, and a sample collection/processing sub-system. He...
Source: Biomedical Microdevices - June 7, 2023 Category: Biomedical Engineering Authors: Jorge Manrique Castro Frank Sommerhage Rishika Khanna Andre Childs David DeRoo Swaminathan Rajaraman Source Type: research
