Microneedle patch-based enzyme-linked immunosorbent assay to quantify protein biomarkers of tuberculosis
This study demonstrates the feasibility of an MNP-based ELISA for differential diagnosis of latent TB disease.PMID:38289481 | DOI:10.1007/s10544-024-00694-2 (Source: Biomedical Microdevices)
Source: Biomedical Microdevices - January 30, 2024 Category: Biomedical Engineering Authors: Youngeun Kim Mary Beth Lewis Jihyun Hwang Zheyu Wang Rohit Gupta Yuxiong Liu Tuhina Gupta James P Barber Srikanth Singamaneni Fred Quinn Mark R Prausnitz Source Type: research
Magnetic nanoparticles fabricated/integrated with microfluidics for biological applications: A review
Biomed Microdevices. 2024 Jan 25;26(1):13. doi: 10.1007/s10544-023-00693-9.ABSTRACTNanostructured materials have gained significant attention in recent years for their potential in biological applications, such as cell and biomolecular sorting, as well as early detection of metastatic cancer. Among these materials, magnetic nanoparticles (MNPs) stand out for their easy functionalization, high specific surface area, chemical stability, and superparamagnetic properties. However, conventional fabrication methods can lead to inconsistencies in MNPs' characteristics and performance, highlighting the need for a cost-effective, c...
Source: Biomedical Microdevices - January 25, 2024 Category: Biomedical Engineering Authors: Mahtab Ghasemi Toudeshkchouei Hassan Abdoos Source Type: research
Metal-based nanoparticles in antibacterial application in biomedical field: Current development and potential mechanisms
Biomed Microdevices. 2024 Jan 23;26(1):12. doi: 10.1007/s10544-023-00686-8.ABSTRACTThe rise in drug resistance in pathogenic bacteria greatly endangers public health in the post-antibiotic era, and drug-resistant bacteria currently pose a great challenge not only to the community but also to clinical procedures, including surgery, stent implantation, organ transplantation, and other medical procedures involving any open wound and compromised human immunity. Biofilm-associated drug failure, as well as rapid resistance to last-resort antibiotics, necessitates the search for novel treatments against bacterial infection. In re...
Source: Biomedical Microdevices - January 23, 2024 Category: Biomedical Engineering Authors: Hao Jiang Lingzhi Li Zhong Li Xiang Chu Source Type: research
Design and development of a portable low-cost QCM-based system for liquid biosensing
This article presents a portable QCM system for liquid biosensing that uses a modified Hartley oscillator to drive 14 mm-diameter commercial QCM sensors. The system is designed to be low-cost, easy to use, and highly sensitive, making it ideal for various bioapplications. A new flow cell design to deliver samples to the surface of the sensor has been designed, fabricated, and tested. For portability and miniaturization purposes, a micropump-based pumping system is used in the current system. The system has a built-in temperature controller allowing for accurate frequency measurements. In addition, the system can be used in...
Source: Biomedical Microdevices - January 18, 2024 Category: Biomedical Engineering Authors: Mohamed Adel Ahmed Allam Ashraf E Sayour Hani F Ragai Shinjiro Umezu Ahmed M R Fath El-Bab Source Type: research
A multiplexed microfluidic continuous-flow electroporation system for efficient cell transfection
Biomed Microdevices. 2024 Jan 9;26(1):10. doi: 10.1007/s10544-023-00692-w.ABSTRACTCellular therapies have the potential to advance treatment for a broad array of diseases but rely on viruses for genetic reprogramming. The time and cost required to produce viruses has created a bottleneck that constricts development of and access to cellular therapies. Electroporation is a non-viral alternative for genetic reprogramming that bypasses these bottlenecks, but current electroporation technology suffers from low throughput, tedious optimization, and difficulty scaling to large-scale cell manufacturing. Here, we present an adapta...
Source: Biomedical Microdevices - January 9, 2024 Category: Biomedical Engineering Authors: Jacob A VanderBurgh Grant T Corso Stephen L Levy Harold G Craighead Source Type: research
A multiplexed microfluidic continuous-flow electroporation system for efficient cell transfection
Biomed Microdevices. 2024 Jan 9;26(1):10. doi: 10.1007/s10544-023-00692-w.ABSTRACTCellular therapies have the potential to advance treatment for a broad array of diseases but rely on viruses for genetic reprogramming. The time and cost required to produce viruses has created a bottleneck that constricts development of and access to cellular therapies. Electroporation is a non-viral alternative for genetic reprogramming that bypasses these bottlenecks, but current electroporation technology suffers from low throughput, tedious optimization, and difficulty scaling to large-scale cell manufacturing. Here, we present an adapta...
Source: Biomedical Microdevices - January 9, 2024 Category: Biomedical Engineering Authors: Jacob A VanderBurgh Grant T Corso Stephen L Levy Harold G Craighead Source Type: research
Fabrication of lidocaine-loaded polymer dissolving microneedles for rapid and prolonged local anesthesia
In this study, a dissolving microneedles (MNs) system made of composite matrix materials of polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), and sodium hyaluronate (HA) was successfully developed for the loading of lidocaine hydrochloride (LidH). The morphology, size and mechanical properties of the MNs were also investigated. After the insertion of MNs into the skin, the matrix at the tip of the MNs was swelled and dissolved by absorption of interstitial fluid, leading to a rapid release of loaded LidH from MNs' tips. And the LidH in the back patching was diffused into deeper skin tissue through microchannels created ...
Source: Biomedical Microdevices - January 8, 2024 Category: Biomedical Engineering Authors: Yanan Mao Xiufeng Zhang Yanfang Sun Zhong Shen Chao Zhong Lei Nie Amin Shavandi Khaydar E Yunusov Guohua Jiang Source Type: research
Fabrication of lidocaine-loaded polymer dissolving microneedles for rapid and prolonged local anesthesia
In this study, a dissolving microneedles (MNs) system made of composite matrix materials of polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), and sodium hyaluronate (HA) was successfully developed for the loading of lidocaine hydrochloride (LidH). The morphology, size and mechanical properties of the MNs were also investigated. After the insertion of MNs into the skin, the matrix at the tip of the MNs was swelled and dissolved by absorption of interstitial fluid, leading to a rapid release of loaded LidH from MNs' tips. And the LidH in the back patching was diffused into deeper skin tissue through microchannels created ...
Source: Biomedical Microdevices - January 8, 2024 Category: Biomedical Engineering Authors: Yanan Mao Xiufeng Zhang Yanfang Sun Zhong Shen Chao Zhong Lei Nie Amin Shavandi Khaydar E Yunusov Guohua Jiang Source Type: research
A disposable impedimetric immunosensor for the analysis of CA125 in human serum samples
Biomed Microdevices. 2024 Jan 5;26(1):8. doi: 10.1007/s10544-023-00691-x.ABSTRACTCancer antigen 125 (CA125) is the most common biomarker used to diagnose and monitor ovarian cancer progression for the last four decades, and precise detection of its levels in blood serum is crucial. In this work, label-free impedimetric CA125 immunosensors were fabricated by using screen-printed carbon electrodes modified with poly toluidine blue (PTB) (in deep eutectic solvent)/gold nanoparticles (AuNP) for the sensitive, environmentally friendly, economical, and practical analysis of CA125. The materials of PTBDES and AuNP were characteri...
Source: Biomedical Microdevices - January 5, 2024 Category: Biomedical Engineering Authors: Merve Y ılmaz Melike Bilgi Source Type: research
A disposable impedimetric immunosensor for the analysis of CA125 in human serum samples
Biomed Microdevices. 2024 Jan 5;26(1):8. doi: 10.1007/s10544-023-00691-x.ABSTRACTCancer antigen 125 (CA125) is the most common biomarker used to diagnose and monitor ovarian cancer progression for the last four decades, and precise detection of its levels in blood serum is crucial. In this work, label-free impedimetric CA125 immunosensors were fabricated by using screen-printed carbon electrodes modified with poly toluidine blue (PTB) (in deep eutectic solvent)/gold nanoparticles (AuNP) for the sensitive, environmentally friendly, economical, and practical analysis of CA125. The materials of PTBDES and AuNP were characteri...
Source: Biomedical Microdevices - January 5, 2024 Category: Biomedical Engineering Authors: Merve Y ılmaz Melike Bilgi Source Type: research
A disposable impedimetric immunosensor for the analysis of CA125 in human serum samples
Biomed Microdevices. 2024 Jan 5;26(1):8. doi: 10.1007/s10544-023-00691-x.ABSTRACTCancer antigen 125 (CA125) is the most common biomarker used to diagnose and monitor ovarian cancer progression for the last four decades, and precise detection of its levels in blood serum is crucial. In this work, label-free impedimetric CA125 immunosensors were fabricated by using screen-printed carbon electrodes modified with poly toluidine blue (PTB) (in deep eutectic solvent)/gold nanoparticles (AuNP) for the sensitive, environmentally friendly, economical, and practical analysis of CA125. The materials of PTBDES and AuNP were characteri...
Source: Biomedical Microdevices - January 5, 2024 Category: Biomedical Engineering Authors: Merve Y ılmaz Melike Bilgi Source Type: research
The effect of magnetic bead size on the isolation efficiency of lung cancer cells in a serpentine microchannel with added cavities
Biomed Microdevices. 2024 Jan 4;26(1):7. doi: 10.1007/s10544-023-00689-5.ABSTRACTAn investigation was conducted to examine the effect of magnetic bead (MB) size on the effectiveness of isolating lung cancer cells using the immunomagnetic separation (IMS) method in a serpentine microchannel with added cavities (SMAC) structure. Carboxylated magnetic beads were specifically conjugated to target cells through a modification procedure using aptamer materials. Cells immobilized with different sizes (in micrometers) of MBs were captured and isolated in the proposed device for comparison and analysis. The study yields significanc...
Source: Biomedical Microdevices - January 4, 2024 Category: Biomedical Engineering Authors: Tzu-Cheng Su Hien Vu-Dinh Shu-Hui Lin Loc Do Quang Trinh Chu Duc Chun-Ping Jen Source Type: research
The effect of magnetic bead size on the isolation efficiency of lung cancer cells in a serpentine microchannel with added cavities
Biomed Microdevices. 2024 Jan 4;26(1):7. doi: 10.1007/s10544-023-00689-5.ABSTRACTAn investigation was conducted to examine the effect of magnetic bead (MB) size on the effectiveness of isolating lung cancer cells using the immunomagnetic separation (IMS) method in a serpentine microchannel with added cavities (SMAC) structure. Carboxylated magnetic beads were specifically conjugated to target cells through a modification procedure using aptamer materials. Cells immobilized with different sizes (in micrometers) of MBs were captured and isolated in the proposed device for comparison and analysis. The study yields significanc...
Source: Biomedical Microdevices - January 4, 2024 Category: Biomedical Engineering Authors: Tzu-Cheng Su Hien Vu-Dinh Shu-Hui Lin Loc Do Quang Trinh Chu Duc Chun-Ping Jen Source Type: research
Rapid-release reversible bonding of PMMA-based microfluidic devices with PBMA coating
In this study, a new approach is proposed for the reversible bonding of PMMA-based microfluidics, a layer of PBMA (with a very similar structure to PMMA) was coated on the surface of PMMA and then use the thermal fusion method to achieve the bonding with a high bonding strength, a tensile bonding strength of around 0.8 MPa was achieved. For debond process, a rapid temperature drop will trigger the immediate release of the bonding within several seconds. Detailed bonding strength measurement and biocompatibility tests were also conducted in this study. The proposed bonding method could have wide application potential in the...
Source: Biomedical Microdevices - December 23, 2023 Category: Biomedical Engineering Authors: Yusheng Li Fan Xu Jing Liu Qi Zhang Yiqiang Fan Source Type: research
Rapid-release reversible bonding of PMMA-based microfluidic devices with PBMA coating
In this study, a new approach is proposed for the reversible bonding of PMMA-based microfluidics, a layer of PBMA (with a very similar structure to PMMA) was coated on the surface of PMMA and then use the thermal fusion method to achieve the bonding with a high bonding strength, a tensile bonding strength of around 0.8 MPa was achieved. For debond process, a rapid temperature drop will trigger the immediate release of the bonding within several seconds. Detailed bonding strength measurement and biocompatibility tests were also conducted in this study. The proposed bonding method could have wide application potential in the...
Source: Biomedical Microdevices - December 23, 2023 Category: Biomedical Engineering Authors: Yusheng Li Fan Xu Jing Liu Qi Zhang Yiqiang Fan Source Type: research
