Mechanical study of alisol B 23-acetate on methionine and choline deficient diet-induced nonalcoholic steatohepatitis based on untargeted metabolomics
This study aimed to investigate the mechanisms underlying the metabolic regulatory effects of AB23A on NASH. We used AB23A to treat mice with NASH, which was induced by a methionine and choline deficient (MCD) diet. We initially investigated therapeutic effect and resistance to oxidation and inflammation of AB23A on NASH. Subsequently, we performed untargeted metabolomic analyses and relative validation assessments to evaluate the metabolic regulatory effects of AB23A. AB23A reduced lipid accumulation, ameliorated oxidative stress and decreased pro-inflammatory cytokines in the liver. Untargeted metabolomic analysis found ...
Source: Biomedical Chromatography : BMC - October 20, 2023 Category: Biomedical Science Authors: Hua-Jun Li Yuan-Song Wang Ya-Nan Wang Ai-Ru Liu Xiu-Hai Su Zi-Ang Ma Li-Xin Wang Zhong-Yong Zhang Shu-Quan Lv Jing Miao Huan-Tian Cui Source Type: research
Mechanical study of alisol B 23-acetate on methionine and choline deficient diet-induced nonalcoholic steatohepatitis based on untargeted metabolomics
This study aimed to investigate the mechanisms underlying the metabolic regulatory effects of AB23A on NASH. We used AB23A to treat mice with NASH, which was induced by a methionine and choline deficient (MCD) diet. We initially investigated therapeutic effect and resistance to oxidation and inflammation of AB23A on NASH. Subsequently, we performed untargeted metabolomic analyses and relative validation assessments to evaluate the metabolic regulatory effects of AB23A. AB23A reduced lipid accumulation, ameliorated oxidative stress and decreased pro-inflammatory cytokines in the liver. Untargeted metabolomic analysis found ...
Source: Biomedical Chromatography : BMC - October 20, 2023 Category: Biomedical Science Authors: Hua-Jun Li Yuan-Song Wang Ya-Nan Wang Ai-Ru Liu Xiu-Hai Su Zi-Ang Ma Li-Xin Wang Zhong-Yong Zhang Shu-Quan Lv Jing Miao Huan-Tian Cui Source Type: research
Mechanical study of alisol B 23-acetate on methionine and choline deficient diet-induced nonalcoholic steatohepatitis based on untargeted metabolomics
This study aimed to investigate the mechanisms underlying the metabolic regulatory effects of AB23A on NASH. We used AB23A to treat mice with NASH, which was induced by a methionine and choline deficient (MCD) diet. We initially investigated therapeutic effect and resistance to oxidation and inflammation of AB23A on NASH. Subsequently, we performed untargeted metabolomic analyses and relative validation assessments to evaluate the metabolic regulatory effects of AB23A. AB23A reduced lipid accumulation, ameliorated oxidative stress and decreased pro-inflammatory cytokines in the liver. Untargeted metabolomic analysis found ...
Source: Biomedical Chromatography : BMC - October 20, 2023 Category: Biomedical Science Authors: Hua-Jun Li Yuan-Song Wang Ya-Nan Wang Ai-Ru Liu Xiu-Hai Su Zi-Ang Ma Li-Xin Wang Zhong-Yong Zhang Shu-Quan Lv Jing Miao Huan-Tian Cui Source Type: research
Mechanical study of alisol B 23-acetate on methionine and choline deficient diet-induced nonalcoholic steatohepatitis based on untargeted metabolomics
This study aimed to investigate the mechanisms underlying the metabolic regulatory effects of AB23A on NASH. We used AB23A to treat mice with NASH, which was induced by a methionine and choline deficient (MCD) diet. We initially investigated therapeutic effect and resistance to oxidation and inflammation of AB23A on NASH. Subsequently, we performed untargeted metabolomic analyses and relative validation assessments to evaluate the metabolic regulatory effects of AB23A. AB23A reduced lipid accumulation, ameliorated oxidative stress and decreased pro-inflammatory cytokines in the liver. Untargeted metabolomic analysis found ...
Source: Biomedical Chromatography : BMC - October 20, 2023 Category: Biomedical Science Authors: Hua-Jun Li Yuan-Song Wang Ya-Nan Wang Ai-Ru Liu Xiu-Hai Su Zi-Ang Ma Li-Xin Wang Zhong-Yong Zhang Shu-Quan Lv Jing Miao Huan-Tian Cui Source Type: research
Differential role of potential stressors, underlying degradation mechanism, characterization of degradants using LC-MS/MS, and establishment of a stability-indicating analytical method for duvelisib
This study is the first to report the structure of degradation impurities and the step-by-step degradation mechanism of DUV. This information will be useful for the scientific community and manufacturers in optimizing the formulation parameters and/or storage conditions. The validated method can be employed for analysis of stability study and routine quality control samples of newer DUV formulations in pharmaceutical industries. The identified impurities may serve as impurity standards for specifying their limits in the drug after required qualification studies.PMID:37852619 | DOI:10.1002/bmc.5760 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - October 18, 2023 Category: Biomedical Science Authors: Nikita Tanaji Tapkir Amit Kumar Sahu Niraj Rajput Tarang Jadav Pinaki Sengupta Source Type: research
Differential role of potential stressors, underlying degradation mechanism, characterization of degradants using LC-MS/MS, and establishment of a stability-indicating analytical method for duvelisib
This study is the first to report the structure of degradation impurities and the step-by-step degradation mechanism of DUV. This information will be useful for the scientific community and manufacturers in optimizing the formulation parameters and/or storage conditions. The validated method can be employed for analysis of stability study and routine quality control samples of newer DUV formulations in pharmaceutical industries. The identified impurities may serve as impurity standards for specifying their limits in the drug after required qualification studies.PMID:37852619 | DOI:10.1002/bmc.5760 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - October 18, 2023 Category: Biomedical Science Authors: Nikita Tanaji Tapkir Amit Kumar Sahu Niraj Rajput Tarang Jadav Pinaki Sengupta Source Type: research
Matrix effect in bioanalytical assay development using supercritical fluid chromatography-mass spectrometry
Biomed Chromatogr. 2023 Oct 16:e5759. doi: 10.1002/bmc.5759. Online ahead of print.ABSTRACTMatrix effect (ME) is commonly caused by coelution of compounds with target analytes, resulting in either suppression or enhancement of analyte ionization. Thus, to achieve the desired accuracy, precision, and sensitivity, ME needs to be evaluated and controlled during bioanalytical method development. As the application of supercritical fluid chromatography-mass spectrometry (SFC-MS) for analysis of biological samples has increased, ME using SFC-MS has also been investigated with a focus on the difference in ME in SFC-MS compared to...
Source: Biomedical Chromatography : BMC - October 17, 2023 Category: Biomedical Science Authors: Liuxi Chen Yuxiang Cui Brian Dean Xiaorong Liang Source Type: research
A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana
In conclusion, data suggest that the method is suitable for the determination of trace Lum in biofluids and can also be used for therapeutic drug monitoring and pharmacokinetic profiling.PMID:37845823 | DOI:10.1002/bmc.5762 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - October 17, 2023 Category: Biomedical Science Authors: Blondie O Brooks Giacomo Maria Paganotti Amos Massele Enoch Sepako Paul Adiukwu Kwenga Festus Sichilongo Source Type: research
Matrix effect in bioanalytical assay development using supercritical fluid chromatography-mass spectrometry
Biomed Chromatogr. 2023 Oct 16:e5759. doi: 10.1002/bmc.5759. Online ahead of print.ABSTRACTMatrix effect (ME) is commonly caused by coelution of compounds with target analytes, resulting in either suppression or enhancement of analyte ionization. Thus, to achieve the desired accuracy, precision, and sensitivity, ME needs to be evaluated and controlled during bioanalytical method development. As the application of supercritical fluid chromatography-mass spectrometry (SFC-MS) for analysis of biological samples has increased, ME using SFC-MS has also been investigated with a focus on the difference in ME in SFC-MS compared to...
Source: Biomedical Chromatography : BMC - October 17, 2023 Category: Biomedical Science Authors: Liuxi Chen Yuxiang Cui Brian Dean Xiaorong Liang Source Type: research
A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana
In conclusion, data suggest that the method is suitable for the determination of trace Lum in biofluids and can also be used for therapeutic drug monitoring and pharmacokinetic profiling.PMID:37845823 | DOI:10.1002/bmc.5762 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - October 17, 2023 Category: Biomedical Science Authors: Blondie O Brooks Giacomo Maria Paganotti Amos Massele Enoch Sepako Paul Adiukwu Kwenga Festus Sichilongo Source Type: research
Development and validation of an LC-MS/MS method for the determination of AZD7648 in rat plasma: Application to a pharmacokinetic study
Biomed Chromatogr. 2023 Oct 16:e5765. doi: 10.1002/bmc.5765. Online ahead of print.ABSTRACTAZD7648 is a potent DNA-PK inhibitor that is being developed for the treatment of ovarian cancer. The study aimed to develop a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine the concentration of AZD7648 in rat. AZD7648 was extracted from plasma by acetonitrile-mediated protein precipitation. The quantification was performed on a Thermo Vantage TSQ mass spectrometer with ibrutinib as an internal standard. A Waters Acquity UPLC BEH C18 column combined with 0.1% aqueous formic acid and...
Source: Biomedical Chromatography : BMC - October 16, 2023 Category: Biomedical Science Authors: Xun Fang Qi Lu Jichun Han Source Type: research
Integrated UHPLC-QE/MS, transcriptomics and network pharmacology reveal the mechanisms via which Liang-Yan-Yi-Zhen-San promotes the browning of white adipose tissue
In this study, we sought to determine which active ingredients of LYYZS mediated its effects on the browning of white adipose tissue. Employing ultra-high performance liquid chromatography-Q-Exactive HF mass spectrometry, a total of 52 LYYZS ingredients were identified. On this basis, 1,560 ingredient-related targets of LYYZS were screened using the HERB databases. Meanwhile, RNA sequencing analysis of the inguinal white adipose tissue of mice produced a total of 3148 genes that were significantly differentially expressed following LYYZS treatment and differentially expressed genes regarded as browning-related targets. Thr...
Source: Biomedical Chromatography : BMC - October 12, 2023 Category: Biomedical Science Authors: Dong Yao Bo Xing Xiang Li Zi-Hua Xu Qian Liu Xin Liu Qiong Wu Ya-Ling Cui Ying Fan Qing-Chun Zhao Source Type: research
Integrated UHPLC-QE/MS, transcriptomics and network pharmacology reveal the mechanisms via which Liang-Yan-Yi-Zhen-San promotes the browning of white adipose tissue
In this study, we sought to determine which active ingredients of LYYZS mediated its effects on the browning of white adipose tissue. Employing ultra-high performance liquid chromatography-Q-Exactive HF mass spectrometry, a total of 52 LYYZS ingredients were identified. On this basis, 1,560 ingredient-related targets of LYYZS were screened using the HERB databases. Meanwhile, RNA sequencing analysis of the inguinal white adipose tissue of mice produced a total of 3148 genes that were significantly differentially expressed following LYYZS treatment and differentially expressed genes regarded as browning-related targets. Thr...
Source: Biomedical Chromatography : BMC - October 12, 2023 Category: Biomedical Science Authors: Dong Yao Bo Xing Xiang Li Zi-Hua Xu Qian Liu Xin Liu Qiong Wu Ya-Ling Cui Ying Fan Qing-Chun Zhao Source Type: research
Integrated UHPLC-QE/MS, transcriptomics and network pharmacology reveal the mechanisms via which Liang-Yan-Yi-Zhen-San promotes the browning of white adipose tissue
In this study, we sought to determine which active ingredients of LYYZS mediated its effects on the browning of white adipose tissue. Employing ultra-high performance liquid chromatography-Q-Exactive HF mass spectrometry, a total of 52 LYYZS ingredients were identified. On this basis, 1,560 ingredient-related targets of LYYZS were screened using the HERB databases. Meanwhile, RNA sequencing analysis of the inguinal white adipose tissue of mice produced a total of 3148 genes that were significantly differentially expressed following LYYZS treatment and differentially expressed genes regarded as browning-related targets. Thr...
Source: Biomedical Chromatography : BMC - October 12, 2023 Category: Biomedical Science Authors: Dong Yao Bo Xing Xiang Li Zi-Hua Xu Qian Liu Xin Liu Qiong Wu Ya-Ling Cui Ying Fan Qing-Chun Zhao Source Type: research
Integrated UHPLC-QE/MS, transcriptomics and network pharmacology reveal the mechanisms via which Liang-Yan-Yi-Zhen-San promotes the browning of white adipose tissue
In this study, we sought to determine which active ingredients of LYYZS mediated its effects on the browning of white adipose tissue. Employing ultra-high performance liquid chromatography-Q-Exactive HF mass spectrometry, a total of 52 LYYZS ingredients were identified. On this basis, 1,560 ingredient-related targets of LYYZS were screened using the HERB databases. Meanwhile, RNA sequencing analysis of the inguinal white adipose tissue of mice produced a total of 3148 genes that were significantly differentially expressed following LYYZS treatment and differentially expressed genes regarded as browning-related targets. Thr...
Source: Biomedical Chromatography : BMC - October 12, 2023 Category: Biomedical Science Authors: Dong Yao Bo Xing Xiang Li Zi-Hua Xu Qian Liu Xin Liu Qiong Wu Ya-Ling Cui Ying Fan Qing-Chun Zhao Source Type: research
