Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study
Biomed Chromatogr. 2023 Dec 14:e5803. doi: 10.1002/bmc.5803. Online ahead of print.ABSTRACTIn this present study, we developed a reliable and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay for the simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin and isomaltopaeoniflorin in beagle dog plasma. We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba (FRPA) in beagle dogs. Plasma samples were processed by protein precipitation with methanol. Chromatographic separation was performed w...
Source: Biomedical Chromatography : BMC - December 15, 2023 Category: Biomedical Science Authors: Wenhao Cheng Zheng Yuan Siyang Wu Xin Yu Kexin Xia Lifeng Zhao Yuyan Wang Chen Kang Wei Yang Luyang Liu Yingfei Li Source Type: research
Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study
Biomed Chromatogr. 2023 Dec 14:e5803. doi: 10.1002/bmc.5803. Online ahead of print.ABSTRACTIn this present study, we developed a reliable and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay for the simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin and isomaltopaeoniflorin in beagle dog plasma. We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba (FRPA) in beagle dogs. Plasma samples were processed by protein precipitation with methanol. Chromatographic separation was performed w...
Source: Biomedical Chromatography : BMC - December 15, 2023 Category: Biomedical Science Authors: Wenhao Cheng Zheng Yuan Siyang Wu Xin Yu Kexin Xia Lifeng Zhao Yuyan Wang Chen Kang Wei Yang Luyang Liu Yingfei Li Source Type: research
Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study
Biomed Chromatogr. 2023 Dec 14:e5803. doi: 10.1002/bmc.5803. Online ahead of print.ABSTRACTIn this present study, we developed a reliable and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay for the simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin and isomaltopaeoniflorin in beagle dog plasma. We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba (FRPA) in beagle dogs. Plasma samples were processed by protein precipitation with methanol. Chromatographic separation was performed w...
Source: Biomedical Chromatography : BMC - December 15, 2023 Category: Biomedical Science Authors: Wenhao Cheng Zheng Yuan Siyang Wu Xin Yu Kexin Xia Lifeng Zhao Yuyan Wang Chen Kang Wei Yang Luyang Liu Yingfei Li Source Type: research
Ultra-high-performance liquid chromatography-quadrupole time of flight tandem mass spectrometry based in vitro metabolite profiling of DK-GV-04P, a novel anticancer molecule under drug discovery
Biomed Chromatogr. 2023 Dec 12:e5806. doi: 10.1002/bmc.5806. Online ahead of print.ABSTRACTDK-GV-04P, chemically identified as 3-cinnamyl-2-(4-methoxyphenyl) quinazolin-4(3H)-one, is an investigational molecule synthesized at the Chemical Biology Laboratory of the National Institute of Pharmaceutical Education and Research-Ahmedabad. The compound has shown potential anticancer activity against squamous CAL27 cell lines. Metabolite identification and characterization are critical in drug discovery, providing key insights into a compound's pharmacokinetics, pharmacodynamics safety, and metabolic fate. The primary aim of the ...
Source: Biomedical Chromatography : BMC - December 13, 2023 Category: Biomedical Science Authors: Deeki Doma Sherpa Amit Kumar Sahu Tarang Jadav Niraj Rajput Gargi Nikhil Vaidya Dinesh Kumar Pinaki Sengupta Source Type: research
Phytochemical analysis and hepatotoxicity assessment of braised Polygoni Multiflori Radix (Wen-He-Shou-Wu)
In this study, we compared the microscopic characteristics of different samples in powder form using scanning electron microscopy (SEM), investigated the non-volatile components, assessed the effects of different processed PMR products on the liver, and compared the differences between BPMR and PMR Praeparata recorded in the Chinese Pharmacopoeia (2020 edition). We found that the hepatotoxicity of BPMR was dramatically decreased, which may be related to an increase in polysaccharide content and a decrease in toxic substances. The present study provides an important foundation for future investigations of the processing mec...
Source: Biomedical Chromatography : BMC - December 13, 2023 Category: Biomedical Science Authors: Tao Zhang Yating Xie Tao Li Yaling Deng Quan Wan Tingting Bai Qing Zhang Zhongxi Cai Mingxia Chen Jinlian Zhang Source Type: research
Ultra-high-performance liquid chromatography-quadrupole time of flight tandem mass spectrometry based in vitro metabolite profiling of DK-GV-04P, a novel anticancer molecule under drug discovery
Biomed Chromatogr. 2023 Dec 12:e5806. doi: 10.1002/bmc.5806. Online ahead of print.ABSTRACTDK-GV-04P, chemically identified as 3-cinnamyl-2-(4-methoxyphenyl) quinazolin-4(3H)-one, is an investigational molecule synthesized at the Chemical Biology Laboratory of the National Institute of Pharmaceutical Education and Research-Ahmedabad. The compound has shown potential anticancer activity against squamous CAL27 cell lines. Metabolite identification and characterization are critical in drug discovery, providing key insights into a compound's pharmacokinetics, pharmacodynamics safety, and metabolic fate. The primary aim of the ...
Source: Biomedical Chromatography : BMC - December 13, 2023 Category: Biomedical Science Authors: Deeki Doma Sherpa Amit Kumar Sahu Tarang Jadav Niraj Rajput Gargi Nikhil Vaidya Dinesh Kumar Pinaki Sengupta Source Type: research
Phytochemical analysis and hepatotoxicity assessment of braised Polygoni Multiflori Radix (Wen-He-Shou-Wu)
In this study, we compared the microscopic characteristics of different samples in powder form using scanning electron microscopy (SEM), investigated the non-volatile components, assessed the effects of different processed PMR products on the liver, and compared the differences between BPMR and PMR Praeparata recorded in the Chinese Pharmacopoeia (2020 edition). We found that the hepatotoxicity of BPMR was dramatically decreased, which may be related to an increase in polysaccharide content and a decrease in toxic substances. The present study provides an important foundation for future investigations of the processing mec...
Source: Biomedical Chromatography : BMC - December 13, 2023 Category: Biomedical Science Authors: Tao Zhang Yating Xie Tao Li Yaling Deng Quan Wan Tingting Bai Qing Zhang Zhongxi Cai Mingxia Chen Jinlian Zhang Source Type: research
UPLC-quadrupole time-of-flight-tandem mass spectrometry combined with chemometrics and network pharmacology to differentiate Coreopsis tinctoria Nutt
In this study, we used ultra-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry to analyze and identify the prevalent chemical components in 12 batches of C. tinctoria sourced from Xinjiang, Qinghai, Tibet, and Yunnan provinces in China. By using cluster analysis and discriminant analysis of partial least squares, we assessed the similarity and identified varying components in the 12 batches of C. tinctoria. Subsequently, their quality was further evaluated. Utilizing network pharmacology, we identified potential active components for the treatment of diabetes mellitus. The findings reveal...
Source: Biomedical Chromatography : BMC - December 12, 2023 Category: Biomedical Science Authors: Xinyu Zhou Wei Cheng Xinmei Chen Kaixuan Wang Source Type: research
Two-dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection
In this study, we developed a simple, precise, and accurate method of two-dimensional liquid chromatography (2D-LC) for determining the concentration of meropenem in human synovial fluid. The method was then validated based on the guidelines of the Food and Drug Administration and the Chinese Pharmacopoeia. Meropenem showed good linearity in the range of 0.31-25.01 μg/mL (r ≥ .999). Selectivity, intra-day and inter-day precision and accuracy, extraction recovery, and stability validation results were all within the acceptance range. This method has been successfully applied to the determination of synovial fluid samples...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: He Jia-Wei Wang Jing Cao Li Zhang Xiao-Gang Li Guo-Qing Xu Bo-Yong Ji Bao-Chao Huang Jun-Jie Zhao Jun Source Type: research
Development and validation of a stability-indicating ultra-high-performance liquid chromatography method for the estimation of ibrutinib and trace-level quantification of related substances using quality-by-design approach
Biomed Chromatogr. 2023 Dec 11:e5798. doi: 10.1002/bmc.5798. Online ahead of print.ABSTRACTA new ultra-high-performance liquid chromatography method was developed using quality-by-design principles for quantifying trace-level impurities of ibrutinib. The method utilized an ACQUITY UPLC BEH C18 column with a mobile phase consisting of equal parts of 0.02 M formic acid in water and 0.02 M formic acid in acetonitrile. The critical method parameters, including mobile phase pH, column temperature, and flow rate, were optimized using the design of experiments. Statistical analysis revealed the impact of these parameters on criti...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: Shishir Kumar Prasad Divekar Kalpana Source Type: research
Development and validation of a sensitive UPLC-MS/MS analytical method for GFH009 in rat plasma and its application to toxicokinetics studies
This study aimed to develop and validate a highly selective and sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for precise quantification of GFH009 in rat plasma. This method was subsequently employed for conducting toxicokinetic studies of GFH009 in rats. Plasma was prepared using a simple protein precipitation method by acetonitrile. Chromatographic separation of the analytes was achieved on a BEH C18 analytical column with a rapid 3.0 min run time and a flow rate of 0.5 ml/min. The calibration curves for plasma samples exhibited excellent linearity over a wide concent...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: Qiang Liu Jin-Zhu Zhao Fu-Sheng Zhou Jiong Lan Qiang Lu Hong-Can Ren Source Type: research
Stability indicating reversed-phase-high-performance liquid chromatography method development and validation for pyridostigmine bromide and sodium benzoate in oral solution
Biomed Chromatogr. 2023 Dec 11:e5800. doi: 10.1002/bmc.5800. Online ahead of print.ABSTRACTThe present study focuses on the development of a simple, rapid, specific, and stability-indicating HPLC method for the simultaneous analysis of pyridostigmine bromide (PGB) and sodium benzoate (SBN) in oral liquid dosage forms. Analytical techniques should enhance sensitivity and specificity for the estimation of pharmaceutical drug products. Stress studies were conducted under various International Conference on Harmonization (ICH) conditions for evaluation. The further optimized HPLC method was validated in accordance with the cur...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: Vaishnavi Chintala Source Type: research
Two-dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection
In this study, we developed a simple, precise, and accurate method of two-dimensional liquid chromatography (2D-LC) for determining the concentration of meropenem in human synovial fluid. The method was then validated based on the guidelines of the Food and Drug Administration and the Chinese Pharmacopoeia. Meropenem showed good linearity in the range of 0.31-25.01 μg/mL (r ≥ .999). Selectivity, intra-day and inter-day precision and accuracy, extraction recovery, and stability validation results were all within the acceptance range. This method has been successfully applied to the determination of synovial fluid samples...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: He Jia-Wei Wang Jing Cao Li Zhang Xiao-Gang Li Guo-Qing Xu Bo-Yong Ji Bao-Chao Huang Jun-Jie Zhao Jun Source Type: research
Development and validation of a stability-indicating ultra-high-performance liquid chromatography method for the estimation of ibrutinib and trace-level quantification of related substances using quality-by-design approach
Biomed Chromatogr. 2023 Dec 11:e5798. doi: 10.1002/bmc.5798. Online ahead of print.ABSTRACTA new ultra-high-performance liquid chromatography method was developed using quality-by-design principles for quantifying trace-level impurities of ibrutinib. The method utilized an ACQUITY UPLC BEH C18 column with a mobile phase consisting of equal parts of 0.02 M formic acid in water and 0.02 M formic acid in acetonitrile. The critical method parameters, including mobile phase pH, column temperature, and flow rate, were optimized using the design of experiments. Statistical analysis revealed the impact of these parameters on criti...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: Shishir Kumar Prasad Divekar Kalpana Source Type: research
Development and validation of a sensitive UPLC-MS/MS analytical method for GFH009 in rat plasma and its application to toxicokinetics studies
This study aimed to develop and validate a highly selective and sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for precise quantification of GFH009 in rat plasma. This method was subsequently employed for conducting toxicokinetic studies of GFH009 in rats. Plasma was prepared using a simple protein precipitation method by acetonitrile. Chromatographic separation of the analytes was achieved on a BEH C18 analytical column with a rapid 3.0 min run time and a flow rate of 0.5 ml/min. The calibration curves for plasma samples exhibited excellent linearity over a wide concent...
Source: Biomedical Chromatography : BMC - December 11, 2023 Category: Biomedical Science Authors: Qiang Liu Jin-Zhu Zhao Fu-Sheng Zhou Jiong Lan Qiang Lu Hong-Can Ren Source Type: research
