Bioavailability assessment of a brompheniramine taste-masked pediatric formulation in a juvenile porcine model
Biomed Chromatogr. 2024 Feb 27:e5845. doi: 10.1002/bmc.5845. Online ahead of print.ABSTRACTA brompheniramine taste-masked pediatric formulation was developed as part of the National Institutes of Health Pediatric Formulation Initiative to help address low patient compliance caused by the bitter taste of many adult formulations. To confirm that the taste-masked formulation can provide a similar pharmacological effect to the previous marketed adult formulations, a juvenile porcine model was used to screen the model pediatric formulation to compare the bioavailability between the marketed brompheniramine maleate and the taste...
Source: Biomedical Chromatography : BMC - February 27, 2024 Category: Biomedical Science Authors: Jiang Wang Diaa Shakleya George Giacoia Ziyuar Rahman Mansoor A Khan Patrick J Faustino Source Type: research
Proteomic analysis of exosomal proteins associated with bone healing speed in a rat tibial fracture model
This study investigates the impact of exosomes on bone fracture healing in a rat tibial model, distinguishing between fast and slow healing processes. Bone healing and protein expression were assessed through X-ray examinations, hematoxylin and eosin staining, and immunohistochemical staining. Exosomes were isolated, characterized and subjected to liquid chromatography-mass spectrometry for protein analysis. Molecular differences were explored using differentially expressed protein analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment and protein-protein interaction networks. Differential bone healing patter...
Source: Biomedical Chromatography : BMC - February 27, 2024 Category: Biomedical Science Authors: Haifeng Hong Changhui Lin Min Fang Juntian Liu Hung-Chih Hsu Chih-Jung Chang Haibing Wang Source Type: research
Bioavailability assessment of a brompheniramine taste-masked pediatric formulation in a juvenile porcine model
Biomed Chromatogr. 2024 Feb 27:e5845. doi: 10.1002/bmc.5845. Online ahead of print.ABSTRACTA brompheniramine taste-masked pediatric formulation was developed as part of the National Institutes of Health Pediatric Formulation Initiative to help address low patient compliance caused by the bitter taste of many adult formulations. To confirm that the taste-masked formulation can provide a similar pharmacological effect to the previous marketed adult formulations, a juvenile porcine model was used to screen the model pediatric formulation to compare the bioavailability between the marketed brompheniramine maleate and the taste...
Source: Biomedical Chromatography : BMC - February 27, 2024 Category: Biomedical Science Authors: Jiang Wang Diaa Shakleya George Giacoia Ziyuar Rahman Mansoor A Khan Patrick J Faustino Source Type: research
Proteomic analysis of exosomal proteins associated with bone healing speed in a rat tibial fracture model
This study investigates the impact of exosomes on bone fracture healing in a rat tibial model, distinguishing between fast and slow healing processes. Bone healing and protein expression were assessed through X-ray examinations, hematoxylin and eosin staining, and immunohistochemical staining. Exosomes were isolated, characterized and subjected to liquid chromatography-mass spectrometry for protein analysis. Molecular differences were explored using differentially expressed protein analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment and protein-protein interaction networks. Differential bone healing patter...
Source: Biomedical Chromatography : BMC - February 27, 2024 Category: Biomedical Science Authors: Haifeng Hong Changhui Lin Min Fang Juntian Liu Hung-Chih Hsu Chih-Jung Chang Haibing Wang Source Type: research
Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy
In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.PMID:38402638 | DOI:10.1002/bmc.5839 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Xiaomin Xu Donghua Yu Yu Wang Peng Xu Xin Jiang Fang Lu Shumin Liu Source Type: research
Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach
This study aims to investigate the protective effect of PDPPAS on chicken liver injury caused by CS combined with LPS buildup and further identify the polysaccharides with the highest hepatoprotective activity in chickens. Furthermore, the study elucidates polysaccharides' intervention mechanism using tandem mass tag (TMT) proteomics and multiple reaction monitoring (MRM) methods. A total of 190 1-day-old layer chickens were randomly assigned into 12 groups, of which 14 chickens were in the control group and 16 in other groups, for a 10-day trial. The screening results showed that charred A. sinensis polysaccharide (CASP) ...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Chen-Chen Li Peng Ji Jian He You-Sheng Peng Fan-Lin Wu Yong-Li Hua Wan-Ling Yao Zi-Wen Yuan Yan-Ming Wei Source Type: research
In vivo and in vitro metabolite profiling of nirmatrelvir using LC-Q-ToF-MS/MS along with the in silico approaches for prediction of metabolites and their toxicity
Biomed Chromatogr. 2024 Feb 25. doi: 10.1002/bmc.5849. Online ahead of print.ABSTRACTNirmatrelvir (NRV), a 3C-like protease or Mpro inhibitor of SARS-CoV-2, is used for the treatment of COVID-19 in adult and paediatric patients. The present study was accomplished to investigate the comprehensive metabolic fate of NRV using in vitro and in vivo models. The in vitro models used for the study were microsomes (human liver microsomes, rat liver microsomes, mouse liver microsomes) and S9 fractions (human liver S9 fractions and rat liver S9 fractions) with the appropriate cofactors, whereas Sprague-Dawley rats were used as the in...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Sowmya Chaganti Bhoopendra Singh Kushwah Laximan Velip Shristy S Tiwari Shrilekha Chilvery Chandraiah Godugu Gananadhamu Samanthula Source Type: research
Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy
In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.PMID:38402638 | DOI:10.1002/bmc.5839 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Xiaomin Xu Donghua Yu Yu Wang Peng Xu Xin Jiang Fang Lu Shumin Liu Source Type: research
Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach
This study aims to investigate the protective effect of PDPPAS on chicken liver injury caused by CS combined with LPS buildup and further identify the polysaccharides with the highest hepatoprotective activity in chickens. Furthermore, the study elucidates polysaccharides' intervention mechanism using tandem mass tag (TMT) proteomics and multiple reaction monitoring (MRM) methods. A total of 190 1-day-old layer chickens were randomly assigned into 12 groups, of which 14 chickens were in the control group and 16 in other groups, for a 10-day trial. The screening results showed that charred A. sinensis polysaccharide (CASP) ...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Chen-Chen Li Peng Ji Jian He You-Sheng Peng Fan-Lin Wu Yong-Li Hua Wan-Ling Yao Zi-Wen Yuan Yan-Ming Wei Source Type: research
In vivo and in vitro metabolite profiling of nirmatrelvir using LC-Q-ToF-MS/MS along with the in silico approaches for prediction of metabolites and their toxicity
Biomed Chromatogr. 2024 Feb 25. doi: 10.1002/bmc.5849. Online ahead of print.ABSTRACTNirmatrelvir (NRV), a 3C-like protease or Mpro inhibitor of SARS-CoV-2, is used for the treatment of COVID-19 in adult and paediatric patients. The present study was accomplished to investigate the comprehensive metabolic fate of NRV using in vitro and in vivo models. The in vitro models used for the study were microsomes (human liver microsomes, rat liver microsomes, mouse liver microsomes) and S9 fractions (human liver S9 fractions and rat liver S9 fractions) with the appropriate cofactors, whereas Sprague-Dawley rats were used as the in...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Sowmya Chaganti Bhoopendra Singh Kushwah Laximan Velip Shristy S Tiwari Shrilekha Chilvery Chandraiah Godugu Gananadhamu Samanthula Source Type: research
Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy
In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.PMID:38402638 | DOI:10.1002/bmc.5839 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Xiaomin Xu Donghua Yu Yu Wang Peng Xu Xin Jiang Fang Lu Shumin Liu Source Type: research
Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach
This study aims to investigate the protective effect of PDPPAS on chicken liver injury caused by CS combined with LPS buildup and further identify the polysaccharides with the highest hepatoprotective activity in chickens. Furthermore, the study elucidates polysaccharides' intervention mechanism using tandem mass tag (TMT) proteomics and multiple reaction monitoring (MRM) methods. A total of 190 1-day-old layer chickens were randomly assigned into 12 groups, of which 14 chickens were in the control group and 16 in other groups, for a 10-day trial. The screening results showed that charred A. sinensis polysaccharide (CASP) ...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Chen-Chen Li Peng Ji Jian He You-Sheng Peng Fan-Lin Wu Yong-Li Hua Wan-Ling Yao Zi-Wen Yuan Yan-Ming Wei Source Type: research
In vivo and in vitro metabolite profiling of nirmatrelvir using LC-Q-ToF-MS/MS along with the in silico approaches for prediction of metabolites and their toxicity
Biomed Chromatogr. 2024 Feb 25. doi: 10.1002/bmc.5849. Online ahead of print.ABSTRACTNirmatrelvir (NRV), a 3C-like protease or Mpro inhibitor of SARS-CoV-2, is used for the treatment of COVID-19 in adult and paediatric patients. The present study was accomplished to investigate the comprehensive metabolic fate of NRV using in vitro and in vivo models. The in vitro models used for the study were microsomes (human liver microsomes, rat liver microsomes, mouse liver microsomes) and S9 fractions (human liver S9 fractions and rat liver S9 fractions) with the appropriate cofactors, whereas Sprague-Dawley rats were used as the in...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Sowmya Chaganti Bhoopendra Singh Kushwah Laximan Velip Shristy S Tiwari Shrilekha Chilvery Chandraiah Godugu Gananadhamu Samanthula Source Type: research
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography-mass spectrometry using phthalaldehyde as the derivatizing agent
Biomed Chromatogr. 2024 Feb 22:e5850. doi: 10.1002/bmc.5850. Online ahead of print.ABSTRACTIn the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC-MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min at room temperature, it reacts with ethylenediamine to form (1z,5z)-3,4-dihydrobenzo[f][1,4]diazocine. This approach minimizes matrix int...
Source: Biomedical Chromatography : BMC - February 22, 2024 Category: Biomedical Science Authors: Sayyad Kousrali Leela Prasad Kowtharapu Tanmoy Mondal Source Type: research
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography-mass spectrometry using phthalaldehyde as the derivatizing agent
Biomed Chromatogr. 2024 Feb 22:e5850. doi: 10.1002/bmc.5850. Online ahead of print.ABSTRACTIn the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC-MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min at room temperature, it reacts with ethylenediamine to form (1z,5z)-3,4-dihydrobenzo[f][1,4]diazocine. This approach minimizes matrix int...
Source: Biomedical Chromatography : BMC - February 22, 2024 Category: Biomedical Science Authors: Sayyad Kousrali Leela Prasad Kowtharapu Tanmoy Mondal Source Type: research
