A simple and sensitive liquid chromatography triple quadrupole mass spectrometry method for the determination of XL092 in monkey plasma and its application to pharmacokinetic study
In this study, an LC triple quadrupole mass spectrometry method was established to measure XL092 in monkey plasma. A Waters ACQUITY UPLC BEH C18 column was used for chromatographic separation. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile with a gradient elution at the flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of XL092 in positive electrospray ionization. In the concentration range of 0.5-1000 ng/mL, XL092 showed excellent linearity in monkey plasma with a correlation coefficient greater than 0.995 (r > 0.995). The lowest limit of qua...
Source: Biomedical Chromatography : BMC - January 31, 2024 Category: Biomedical Science Authors: Cui Li Xiaokun Li Ali Fan Ning He Dongmei Wu Hongyan Yu Kun Wang Weijie Jiao Xu Zhao Source Type: research
Mycotoxin detection in selected medicinal plants using chromatographic techniques
Biomed Chromatogr. 2024 Jan 30. doi: 10.1002/bmc.5831. Online ahead of print.ABSTRACTMycotoxins are toxic mycological products that when consumed, absorbed or inhaled cause sickness or even the death of humans. Therefore, the present study aimed to evaluate the contamination levels of mycotoxins (aflatoxins, AFB1 , AFB2 , AFG1 , AFG2 , and ochratoxin A, OTA) in selected medicinal herbs and shrubs using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). A total of 15 samples of medicinal herbs and shrubs were selected. Among them, four samples were aflatoxin contaminated while two samples wer...
Source: Biomedical Chromatography : BMC - January 31, 2024 Category: Biomedical Science Authors: Ihsan Ul Haq Raheela Taj Muhammad Nafees Arshad Hussain Source Type: research
Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats
Biomed Chromatogr. 2024 Jan 29:e5827. doi: 10.1002/bmc.5827. Online ahead of print.ABSTRACTIn recent years, researchers have shown a growing interest in the interactions between different pharmaceutical agents. An intriguing instance lies in the possible interaction between nimodipine and vitamin C. To investigate the pharmacokinetic and pharmacodynamic effects of vitamin C on nimodipine in rats, rats were randomly divided into a nimodipine only group and a combination group (nimodipine + vitamin C). The two groups were given intragastric administration and nimodipine blood concentrations were determined using high-perform...
Source: Biomedical Chromatography : BMC - January 29, 2024 Category: Biomedical Science Authors: Xian-Xin Meng Guan-Jun Nan Hao Hu Jian-Yu He Yun-Zhe Li Guang-de Yang Source Type: research
Pharmacokinetics, absolute bioavailability, and tissue distribution of WJ-14, a novel N-methyl-d-aspartate receptor antagonist, in rats by liquid chromatography-tandem mass spectrometry
Biomed Chromatogr. 2024 Jan 22:e5823. doi: 10.1002/bmc.5823. Online ahead of print.ABSTRACTTo circumvent the limitations of current antidepressants, WJ-14, a novel N-methyl-d-aspartate receptor antagonist, was synthesized and demonstrated to have remarkable efficiency in the treatment of depression. To illustrate the pharmacokinetics, absolute bioavailability, and tissue distribution of WJ-14 in rats, a rapid and sensitive liquid chromatography-tandem mass spectrometry-based analytical method was developed and validated for the separation and detection of WJ-14 in both plasma and tissue samples. After oral administration, ...
Source: Biomedical Chromatography : BMC - January 23, 2024 Category: Biomedical Science Authors: Weiyin Huang Yan Zhang Lishou Yang Yiwen Zhang Lei Cheng Yan Deng Xinyu Wu Tingting Wang Xiaosheng Yang Linhu Ye Source Type: research
Liquid chromatography-tandem mass spectrometry determination of bumetanide in human plasma and application to a clinical pharmacokinetic study
This study aimed to develop and validate a specific, simple, sensitive, and accurate method using liquid chromatography-tandem mass spectrometry (LC-MS) for measuring bumetanide (BUM) in human plasma. Chromatographic separation was achieved using a Hypurity C18 column (4.6 × 50 mm, 5 μm) under isocratic conditions, and LC-MS detected positive ionization acquisition modes. Protonated precursor to product ion transitions were observed at m/z 365.08 → 240.10 and 370.04 → 244.52 for BUM and internal standard, respectively. The linear range of BUM in plasma samples was 3.490-401.192 ng/mL. The inter-precision value ranged...
Source: Biomedical Chromatography : BMC - January 18, 2024 Category: Biomedical Science Authors: Ganesan Padmini Tamilarasi Krishnan Manikandan Viswas Raja Solomon Source Type: research
Integration of metabolomics and network pharmacology technology to explain the effect mechanisms of Danggui Buxue decoction in vascular dementia
Biomed Chromatogr. 2024 Jan 18:e5822. doi: 10.1002/bmc.5822. Online ahead of print.ABSTRACTDanggui Buxue decoction (DBD) is a traditional Chinese medicine herbal decoction that has a good therapeutic effect on vascular dementia (VaD). However, its pharmacodynamic substances and underlying mechanisms are ambiguous. The work aimed to decipher the pharmacodynamic substances and molecular mechanisms of DBD against VaD rats based on gas chromatography-mass spectrometry metabonomics, network pharmacology, molecular docking, and experimental verification. The results indicated that DBD significantly improved the learning abilitie...
Source: Biomedical Chromatography : BMC - January 18, 2024 Category: Biomedical Science Authors: Qin Fan Xinhong Liu Yanying Zhang Wanrong Kang Shanshan Si Hongmei Zhang Source Type: research
Structure of crude polysaccharides from Atractylodes lancea rhizome and treatment of diarrhea owing to spleen deficiency through intestinal flora
Biomed Chromatogr. 2024 Jan 17:e5818. doi: 10.1002/bmc.5818. Online ahead of print.ABSTRACTTo optimize the extraction process of crude polysaccharides from Atractylodes and elaborate the mechanism of Atractylodes polysaccharides in treating diarrhea owing to spleen deficiency, so as to lay a foundation for further development and utilization of Atractylodes lancea, we used an orthogonal test to optimize the extraction process and established a model of spleen deficiency. It was further combined with histopathology and intestinal flora to elaborate the mechanism of Atractylodes polysaccharides in the treatment of spleen-def...
Source: Biomedical Chromatography : BMC - January 17, 2024 Category: Biomedical Science Authors: Yue Wang Sheng Wang Tie-Lin Wang Li-Kun Chang Jun-Hui Zhou Ke Yang Lan-Ping Guo Source Type: research
Development of a novel quality by design-enabled stability-indicating HPLC method and its validation for the quantification of nirmatrelvir in bulk and pharmaceutical dosage forms
Biomed Chromatogr. 2024 Jan 16:e5812. doi: 10.1002/bmc.5812. Online ahead of print.ABSTRACTA systematic and novel quality by design-enabled, rapid, simple, and economic stability-indicating HPLC method for quantifying nirmatrelvir (NMT) was successfully developed and validated. An analytical target profile (ATP) was established, and critical analytical attributes (CAAs) were allocated to meet the ATP requirements. The method used chromatographic separation using a Purosphere column with a 4.6 mm inner diameter × 250 mm (2.5 μm). The analysis occurred at 50°C with a flow rate of 1.2 mL/min and detection at 220 nm. A 10 Î...
Source: Biomedical Chromatography : BMC - January 16, 2024 Category: Biomedical Science Authors: Pallavi Alegete Saisudha Byreddy Source Type: research
Development of an ultra-high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma and application in their pharmacokinetics
Biomed Chromatogr. 2024 Jan 13:e5821. doi: 10.1002/bmc.5821. Online ahead of print.ABSTRACTIn this paper, an ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for quantifying the levels of crassicauline A, fuziline, karacoline, and songorine in rat plasma. After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile-methanol (9:1, v/v). The chromatographic column used was the UPLC HSS T3 column, and the mobile phase (methanol-water with 0.1% formic acid) under a gradient elution profile was used to separate the ...
Source: Biomedical Chromatography : BMC - January 13, 2024 Category: Biomedical Science Authors: Fan Chen Ziyue Wang Lvqi Luo Yifan He Yizhe Ma Congcong Wen Xianqin Wang Xiuwei Shen Source Type: research
Multimodal integrated strategy for the discovery and identification of antiplatelet aggregation Q-markers in Paris polyphylla var. yunnanensis
This study uses a representative Chinese medicine with multi-origin and multi-efficacy, Paris polyphylla var. yunnanensis (PY), as an illustrative example. Surprisingly, despite the high fingerprint similarity among the 12 batches of PY samples collected from various regions in Yunnan, a notable variation in the composition and content of components was observed. The chromatographic analysis identified seven common peaks, namely, polyphyllin I, polyphyllin II, polyphyllin V, polyphyllin VI, polyphyllin VII, polyphyllin H, and polyphyllin D. In the bioactivity evaluation, an in vitro antiplatelet aggregation model induced b...
Source: Biomedical Chromatography : BMC - January 12, 2024 Category: Biomedical Science Authors: Yang Li Rouyuan Wen Wanqing Yang Huimei Xu Qiufeng Xie Le Wang Hanzhu Sun Haizhu Zhang Conglong Xia Source Type: research
Insight gained via ultra-performance liquid chromatography tandem mass spectrometry-based metabolomics: Protective effect of Artemisia argyi H.L év. & amp; Vaniot essential oil on lipopolysaccharide-induced liver injury mice
In this study, we used ultra-performance liquid chromatography tandem mass spectrometry metabolomics techniques to investigate the changes in liver tissue metabolites in mice exposed to LPS with or without AAEO treatment for 14 days. The biochemical results showed that compared with the control group, AAEO significantly reduced the levels of liver functional enzymes, suggesting a significant improvement in liver injury. In addition, the 18 differential metabolites identified by metabolomics were mainly involved in the reprogramming of arachidonic acid metabolism, tryptophan metabolism, and purine metabolism. AAEO could sig...
Source: Biomedical Chromatography : BMC - January 11, 2024 Category: Biomedical Science Authors: Hui Zhou Ying Rong Fanglin Liu Tong Yu Weiqi Cui Qianwen Cao Luyao Liu Zhaolong Qin Xiaoge Ma Nan Zhang Youcai Tang Xia Xu Source Type: research
Comparative phytochemical study of methanolic and ethanolic extracts of Thymus linearis and their antibacterial and antioxidant potential
Biomed Chromatogr. 2024 Jan 8:e5808. doi: 10.1002/bmc.5808. Online ahead of print.ABSTRACTThymus linearis (Thyme) is a medicinal plant widely distributed throughout Asia. Various parts of thyme are utilized for diverse medicinal purposes, including its use as a tonic and diuretic, for cough relief, as a flavoring agent, in treating dysentery, and for alleviating stomach disorders. Numerous studies have been conducted to explore the unexploited potential of thyme. Thyme was collected from the northern region of Pakistan, and sun-mediated extraction was conducted. Phytochemical analysis, utilizing GC-MS, revealed numerous bi...
Source: Biomedical Chromatography : BMC - January 9, 2024 Category: Biomedical Science Authors: Hafiz Amir Nadeem Muhammad Pervaiz Anam Ejaz Zohaib Saeed Muhammad Imran Rana Rashad Mahmood Khan Umer Younas Source Type: research
Comparative phytochemical study of methanolic and ethanolic extracts of Thymus linearis and their antibacterial and antioxidant potential
Biomed Chromatogr. 2024 Jan 8:e5808. doi: 10.1002/bmc.5808. Online ahead of print.ABSTRACTThymus linearis (Thyme) is a medicinal plant widely distributed throughout Asia. Various parts of thyme are utilized for diverse medicinal purposes, including its use as a tonic and diuretic, for cough relief, as a flavoring agent, in treating dysentery, and for alleviating stomach disorders. Numerous studies have been conducted to explore the unexploited potential of thyme. Thyme was collected from the northern region of Pakistan, and sun-mediated extraction was conducted. Phytochemical analysis, utilizing GC-MS, revealed numerous bi...
Source: Biomedical Chromatography : BMC - January 9, 2024 Category: Biomedical Science Authors: Hafiz Amir Nadeem Muhammad Pervaiz Anam Ejaz Zohaib Saeed Muhammad Imran Rana Rashad Mahmood Khan Umer Younas Source Type: research
Effect of vinegar steaming on the composition and structure of Schisandra chinensis polysaccharide and its anti-colitis activity
This study showed that the apparent morphology and molecular weight of S. chinensis's polysaccharides significantly changed after steaming with vinegar. These changes directly affect its anti-UC effect significantly, and its mechanism is closely related to improving the structure and diversity of gut microbiota and SCFA metabolism.PMID:38191780 | DOI:10.1002/bmc.5811 (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - January 8, 2024 Category: Biomedical Science Authors: Zhi-Jun Guo Wei Zhang Jin-Guo Xu Xiao-Man Li Jiu-Ba Zhang Yu Li De Ji Lin Li Wei Huang Lian-Lin Su Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
