Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection
Biomed Chromatogr. 2023 Dec 30:e5790. doi: 10.1002/bmc.5790. Online ahead of print.ABSTRACTIn the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four impurities were methyl 4-methylbenzenesulfonate (PGI-1), ethyl 4-methylbenzenesulfonate (PGI-2), 2-hydroxyethyl 4-methylbenzenesulfonate...
Source: Biomedical Chromatography : BMC - December 30, 2023 Category: Biomedical Science Authors: Tingyu Xiao Huaqing Lin Jiekeng Lao Xin Hu Yucheng Chen Yicong Lei Mingzhi Xu Source Type: research
Development of a simultaneous quantification method of imatinib and sunitinib and their main metabolites and its application in patients with gastrointestinal stromal tumor
Biomed Chromatogr. 2023 Dec 28:e5804. doi: 10.1002/bmc.5804. Online ahead of print.ABSTRACTCorrelations between plasma concentrations of imatinib and sunitinib with efficacy and toxicity have been established. It is crucial to develop a sensitive and precise method for determining the plasma concentrations of imatinib and sunitinib, along with their active metabolites, to facilitate therapeutic drug monitoring and individualized therapy. Plasma samples were separated on an Agilent ZORBAX SB-C18 chromatographic column using gradient elution. Quantification was performed using a mass spectrometer equipped with electrospray i...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Hefen Chen Jianbo Lyu Zhujun Gong Yong Han Kaixiong Tao Hong Zhou Source Type: research
Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy
In this study, we evaluated the metabolic profile during the epileptogenesis periods. Using a pilocarpine model of epilepsy, we analyzed the global metabolic profile of hippocampal extracts by untargeted metabolomics based on ultra-performance liquid chromatography-high-resolution mass spectrometry, at three time points (3 h, 1 week, and 2 weeks) after status epilepticus (SE) induction. We demonstrated that epileptogenesis periods presented different hippocampal metabolic profiles, including alterations of metabolic pathways of amino acids and lipid metabolism. Six putative metabolites (tryptophan, N-acetylornithine, N-ace...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Let ícia Meier Estevan Bruginski Joseane Righes Marafiga Let ícia Barbieri Caus Mayara Vendramin Pasquetti Maria Elisa Calcagnotto Francinete Ramos Campos Source Type: research
Development of a simultaneous quantification method of imatinib and sunitinib and their main metabolites and its application in patients with gastrointestinal stromal tumor
Biomed Chromatogr. 2023 Dec 28:e5804. doi: 10.1002/bmc.5804. Online ahead of print.ABSTRACTCorrelations between plasma concentrations of imatinib and sunitinib with efficacy and toxicity have been established. It is crucial to develop a sensitive and precise method for determining the plasma concentrations of imatinib and sunitinib, along with their active metabolites, to facilitate therapeutic drug monitoring and individualized therapy. Plasma samples were separated on an Agilent ZORBAX SB-C18 chromatographic column using gradient elution. Quantification was performed using a mass spectrometer equipped with electrospray i...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Hefen Chen Jianbo Lyu Zhujun Gong Yong Han Kaixiong Tao Hong Zhou Source Type: research
Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy
In this study, we evaluated the metabolic profile during the epileptogenesis periods. Using a pilocarpine model of epilepsy, we analyzed the global metabolic profile of hippocampal extracts by untargeted metabolomics based on ultra-performance liquid chromatography-high-resolution mass spectrometry, at three time points (3 h, 1 week, and 2 weeks) after status epilepticus (SE) induction. We demonstrated that epileptogenesis periods presented different hippocampal metabolic profiles, including alterations of metabolic pathways of amino acids and lipid metabolism. Six putative metabolites (tryptophan, N-acetylornithine, N-ace...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Let ícia Meier Estevan Bruginski Joseane Righes Marafiga Let ícia Barbieri Caus Mayara Vendramin Pasquetti Maria Elisa Calcagnotto Francinete Ramos Campos Source Type: research
Development of a simultaneous quantification method of imatinib and sunitinib and their main metabolites and its application in patients with gastrointestinal stromal tumor
Biomed Chromatogr. 2023 Dec 28:e5804. doi: 10.1002/bmc.5804. Online ahead of print.ABSTRACTCorrelations between plasma concentrations of imatinib and sunitinib with efficacy and toxicity have been established. It is crucial to develop a sensitive and precise method for determining the plasma concentrations of imatinib and sunitinib, along with their active metabolites, to facilitate therapeutic drug monitoring and individualized therapy. Plasma samples were separated on an Agilent ZORBAX SB-C18 chromatographic column using gradient elution. Quantification was performed using a mass spectrometer equipped with electrospray i...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Hefen Chen Jianbo Lyu Zhujun Gong Yong Han Kaixiong Tao Hong Zhou Source Type: research
Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy
In this study, we evaluated the metabolic profile during the epileptogenesis periods. Using a pilocarpine model of epilepsy, we analyzed the global metabolic profile of hippocampal extracts by untargeted metabolomics based on ultra-performance liquid chromatography-high-resolution mass spectrometry, at three time points (3 h, 1 week, and 2 weeks) after status epilepticus (SE) induction. We demonstrated that epileptogenesis periods presented different hippocampal metabolic profiles, including alterations of metabolic pathways of amino acids and lipid metabolism. Six putative metabolites (tryptophan, N-acetylornithine, N-ace...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Let ícia Meier Estevan Bruginski Joseane Righes Marafiga Let ícia Barbieri Caus Mayara Vendramin Pasquetti Maria Elisa Calcagnotto Francinete Ramos Campos Source Type: research
Development of a simultaneous quantification method of imatinib and sunitinib and their main metabolites and its application in patients with gastrointestinal stromal tumor
Biomed Chromatogr. 2023 Dec 28:e5804. doi: 10.1002/bmc.5804. Online ahead of print.ABSTRACTCorrelations between plasma concentrations of imatinib and sunitinib with efficacy and toxicity have been established. It is crucial to develop a sensitive and precise method for determining the plasma concentrations of imatinib and sunitinib, along with their active metabolites, to facilitate therapeutic drug monitoring and individualized therapy. Plasma samples were separated on an Agilent ZORBAX SB-C18 chromatographic column using gradient elution. Quantification was performed using a mass spectrometer equipped with electrospray i...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Hefen Chen Jianbo Lyu Zhujun Gong Yong Han Kaixiong Tao Hong Zhou Source Type: research
Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy
In this study, we evaluated the metabolic profile during the epileptogenesis periods. Using a pilocarpine model of epilepsy, we analyzed the global metabolic profile of hippocampal extracts by untargeted metabolomics based on ultra-performance liquid chromatography-high-resolution mass spectrometry, at three time points (3 h, 1 week, and 2 weeks) after status epilepticus (SE) induction. We demonstrated that epileptogenesis periods presented different hippocampal metabolic profiles, including alterations of metabolic pathways of amino acids and lipid metabolism. Six putative metabolites (tryptophan, N-acetylornithine, N-ace...
Source: Biomedical Chromatography : BMC - December 28, 2023 Category: Biomedical Science Authors: Let ícia Meier Estevan Bruginski Joseane Righes Marafiga Let ícia Barbieri Caus Mayara Vendramin Pasquetti Maria Elisa Calcagnotto Francinete Ramos Campos Source Type: research
Optimization and validation of high-performance liquid chromatography using modified QuEChERS to determine anthelmintic drugs in mutton
Biomed Chromatogr. 2023 Dec 26:e5814. doi: 10.1002/bmc.5814. Online ahead of print.ABSTRACTThe aim of this study was to rapidly determine the presence of anthelmintic drugs in sheep meat using the optimized high-performance liquid chromatography-ultraviolet (HPLC-UV) method with modified QuEChERS (quick, easy, cheap, effective, rugged, safe) technology. Fifty fresh sheep meat samples from different slaughterhouses were collected. A double extraction procedure (QuEChERS/HPLC-UV technology) was used to extract the target analytes. A multilevel calibration curve from 1 to 1000 g/kg was used to establish instrument linearity f...
Source: Biomedical Chromatography : BMC - December 27, 2023 Category: Biomedical Science Authors: Hiwa Muhammad Husein Hiwa Khulod Ibraheem Hassan Khulod Source Type: research
