Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach
This study aims to investigate the protective effect of PDPPAS on chicken liver injury caused by CS combined with LPS buildup and further identify the polysaccharides with the highest hepatoprotective activity in chickens. Furthermore, the study elucidates polysaccharides' intervention mechanism using tandem mass tag (TMT) proteomics and multiple reaction monitoring (MRM) methods. A total of 190 1-day-old layer chickens were randomly assigned into 12 groups, of which 14 chickens were in the control group and 16 in other groups, for a 10-day trial. The screening results showed that charred A. sinensis polysaccharide (CASP) ...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Chen-Chen Li Peng Ji Jian He You-Sheng Peng Fan-Lin Wu Yong-Li Hua Wan-Ling Yao Zi-Wen Yuan Yan-Ming Wei Source Type: research
In vivo and in vitro metabolite profiling of nirmatrelvir using LC-Q-ToF-MS/MS along with the in silico approaches for prediction of metabolites and their toxicity
Biomed Chromatogr. 2024 Feb 25. doi: 10.1002/bmc.5849. Online ahead of print.ABSTRACTNirmatrelvir (NRV), a 3C-like protease or Mpro inhibitor of SARS-CoV-2, is used for the treatment of COVID-19 in adult and paediatric patients. The present study was accomplished to investigate the comprehensive metabolic fate of NRV using in vitro and in vivo models. The in vitro models used for the study were microsomes (human liver microsomes, rat liver microsomes, mouse liver microsomes) and S9 fractions (human liver S9 fractions and rat liver S9 fractions) with the appropriate cofactors, whereas Sprague-Dawley rats were used as the in...
Source: Biomedical Chromatography : BMC - February 25, 2024 Category: Biomedical Science Authors: Sowmya Chaganti Bhoopendra Singh Kushwah Laximan Velip Shristy S Tiwari Shrilekha Chilvery Chandraiah Godugu Gananadhamu Samanthula Source Type: research
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography-mass spectrometry using phthalaldehyde as the derivatizing agent
Biomed Chromatogr. 2024 Feb 22:e5850. doi: 10.1002/bmc.5850. Online ahead of print.ABSTRACTIn the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC-MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min at room temperature, it reacts with ethylenediamine to form (1z,5z)-3,4-dihydrobenzo[f][1,4]diazocine. This approach minimizes matrix int...
Source: Biomedical Chromatography : BMC - February 22, 2024 Category: Biomedical Science Authors: Sayyad Kousrali Leela Prasad Kowtharapu Tanmoy Mondal Source Type: research
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography-mass spectrometry using phthalaldehyde as the derivatizing agent
Biomed Chromatogr. 2024 Feb 22:e5850. doi: 10.1002/bmc.5850. Online ahead of print.ABSTRACTIn the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC-MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min at room temperature, it reacts with ethylenediamine to form (1z,5z)-3,4-dihydrobenzo[f][1,4]diazocine. This approach minimizes matrix int...
Source: Biomedical Chromatography : BMC - February 22, 2024 Category: Biomedical Science Authors: Sayyad Kousrali Leela Prasad Kowtharapu Tanmoy Mondal Source Type: research
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography-mass spectrometry using phthalaldehyde as the derivatizing agent
Biomed Chromatogr. 2024 Feb 22:e5850. doi: 10.1002/bmc.5850. Online ahead of print.ABSTRACTIn the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC-MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min at room temperature, it reacts with ethylenediamine to form (1z,5z)-3,4-dihydrobenzo[f][1,4]diazocine. This approach minimizes matrix int...
Source: Biomedical Chromatography : BMC - February 22, 2024 Category: Biomedical Science Authors: Sayyad Kousrali Leela Prasad Kowtharapu Tanmoy Mondal Source Type: research
Chromatographic authentication of botanical origin: Herbaceous pollen profiling with HPLC, HPTLC and GC-MS analysis
This study describes a robust chromatographic authentication methodology for herbaceous pollen, employing gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC) and high-performance thin liquid chromatography (HPTLC) protocols. The comprehensive profiling of organic compounds not only distinguishes between different botanical sources but also establishes a reliable framework for quality control and assessment of herbaceous pollen authenticity. Traces of quercetin were detectable using HPTLC in Chaenomeles japonica, and the composition of the mobile phase led to distinct phenolic acid tr...
Source: Biomedical Chromatography : BMC - February 21, 2024 Category: Biomedical Science Authors: Nozimova Aziza Khislat Khaydarov Muhammad Zafar Waleed A A Alsakkaf Jawaher Alkahtani Mushtaq Ahmad Trobjon Makhkamov Zamira Djumayeva Gokhan Zengin Tursunboev Khamdam Eshboyevich Aferin Beilerli Ilgiz Gareev Ulugbek Ochilov Islamov Boston Sultanovich Umu Source Type: research
Spectrum-effect relationship study between ultra-high-performance liquid chromatography fingerprints and anti-hepatoma effect in vitro of Cnidii Fructus
This study established the spectrum-effect relationships between ultra-high-performance liquid chromatography (UHPLC) fingerprints and the antitumor activities of Cnidii Fructus on human hepatocellular carcinoma (HepG2) cells. In UHPLC fingerprints, 19 common peaks were obtained, and 17 batches of herbs had similarity >0.948. In Cell Counting Kit-8 (CCK-8) test, 17 batches of Cnidii Fructus extract significantly inhibited the proliferation of HepG2 cells to different degrees, showing different half-maximal inhibitory concentration (IC50 ) values. Furthermore, gray correlation analysis, Pearson's analysis, and orthogonal...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Zhenguo Lv Huizi Ouyang Fanjiao Zuo Minglei Ge Mengxuan Wu Lulu Zhao Yameng Zhu Xinxin Miao Yu Bai Yanxu Chang Jun He Source Type: research
Investigation of the therapeutic effect of Hedan tablets on high-fat diet-induced obesity in rats by GC-MS technology and 16S ribosomal RNA gene sequencing
This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue pathology. Furthermore, we utilized 16S ribosomal RNA gene sequencing technology to explore changes in intestinal flora. In addition, GC-MS was used to measure fecal short-chain fatty acid (SCFA) content. The onset of obesity led to a significant decrease in the relative abundance of beneficial bacteria. Conversely, the administration of HDT demonstra...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Xuan Pan Yumeng Zhang Yongyao Qiao Qingying Cao Liuxin Wei Min Zhao Source Type: research
Spectrum-effect relationship study between ultra-high-performance liquid chromatography fingerprints and anti-hepatoma effect in vitro of Cnidii Fructus
This study established the spectrum-effect relationships between ultra-high-performance liquid chromatography (UHPLC) fingerprints and the antitumor activities of Cnidii Fructus on human hepatocellular carcinoma (HepG2) cells. In UHPLC fingerprints, 19 common peaks were obtained, and 17 batches of herbs had similarity >0.948. In Cell Counting Kit-8 (CCK-8) test, 17 batches of Cnidii Fructus extract significantly inhibited the proliferation of HepG2 cells to different degrees, showing different half-maximal inhibitory concentration (IC50 ) values. Furthermore, gray correlation analysis, Pearson's analysis, and orthogonal...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Zhenguo Lv Huizi Ouyang Fanjiao Zuo Minglei Ge Mengxuan Wu Lulu Zhao Yameng Zhu Xinxin Miao Yu Bai Yanxu Chang Jun He Source Type: research
Investigation of the therapeutic effect of Hedan tablets on high-fat diet-induced obesity in rats by GC-MS technology and 16S ribosomal RNA gene sequencing
This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue pathology. Furthermore, we utilized 16S ribosomal RNA gene sequencing technology to explore changes in intestinal flora. In addition, GC-MS was used to measure fecal short-chain fatty acid (SCFA) content. The onset of obesity led to a significant decrease in the relative abundance of beneficial bacteria. Conversely, the administration of HDT demonstra...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Xuan Pan Yumeng Zhang Yongyao Qiao Qingying Cao Liuxin Wei Min Zhao Source Type: research
Spectrum-effect relationship study between ultra-high-performance liquid chromatography fingerprints and anti-hepatoma effect in vitro of Cnidii Fructus
This study established the spectrum-effect relationships between ultra-high-performance liquid chromatography (UHPLC) fingerprints and the antitumor activities of Cnidii Fructus on human hepatocellular carcinoma (HepG2) cells. In UHPLC fingerprints, 19 common peaks were obtained, and 17 batches of herbs had similarity >0.948. In Cell Counting Kit-8 (CCK-8) test, 17 batches of Cnidii Fructus extract significantly inhibited the proliferation of HepG2 cells to different degrees, showing different half-maximal inhibitory concentration (IC50 ) values. Furthermore, gray correlation analysis, Pearson's analysis, and orthogonal...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Zhenguo Lv Huizi Ouyang Fanjiao Zuo Minglei Ge Mengxuan Wu Lulu Zhao Yameng Zhu Xinxin Miao Yu Bai Yanxu Chang Jun He Source Type: research
Investigation of the therapeutic effect of Hedan tablets on high-fat diet-induced obesity in rats by GC-MS technology and 16S ribosomal RNA gene sequencing
This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue pathology. Furthermore, we utilized 16S ribosomal RNA gene sequencing technology to explore changes in intestinal flora. In addition, GC-MS was used to measure fecal short-chain fatty acid (SCFA) content. The onset of obesity led to a significant decrease in the relative abundance of beneficial bacteria. Conversely, the administration of HDT demonstra...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Xuan Pan Yumeng Zhang Yongyao Qiao Qingying Cao Liuxin Wei Min Zhao Source Type: research
Spectrum-effect relationship study between ultra-high-performance liquid chromatography fingerprints and anti-hepatoma effect in vitro of Cnidii Fructus
This study established the spectrum-effect relationships between ultra-high-performance liquid chromatography (UHPLC) fingerprints and the antitumor activities of Cnidii Fructus on human hepatocellular carcinoma (HepG2) cells. In UHPLC fingerprints, 19 common peaks were obtained, and 17 batches of herbs had similarity >0.948. In Cell Counting Kit-8 (CCK-8) test, 17 batches of Cnidii Fructus extract significantly inhibited the proliferation of HepG2 cells to different degrees, showing different half-maximal inhibitory concentration (IC50 ) values. Furthermore, gray correlation analysis, Pearson's analysis, and orthogonal...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Zhenguo Lv Huizi Ouyang Fanjiao Zuo Minglei Ge Mengxuan Wu Lulu Zhao Yameng Zhu Xinxin Miao Yu Bai Yanxu Chang Jun He Source Type: research
Investigation of the therapeutic effect of Hedan tablets on high-fat diet-induced obesity in rats by GC-MS technology and 16S ribosomal RNA gene sequencing
This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue pathology. Furthermore, we utilized 16S ribosomal RNA gene sequencing technology to explore changes in intestinal flora. In addition, GC-MS was used to measure fecal short-chain fatty acid (SCFA) content. The onset of obesity led to a significant decrease in the relative abundance of beneficial bacteria. Conversely, the administration of HDT demonstra...
Source: Biomedical Chromatography : BMC - February 18, 2024 Category: Biomedical Science Authors: Xuan Pan Yumeng Zhang Yongyao Qiao Qingying Cao Liuxin Wei Min Zhao Source Type: research
LC-MS/MS method for the quantification of cortisol of hepatocellular carcinoma
Biomed Chromatogr. 2024 Feb 13:e5829. doi: 10.1002/bmc.5829. Online ahead of print.ABSTRACTThe imbalance of steroid hormones is closely related to the occurrence and development of hepatocellular carcinoma (HCC). However, most research has focused on steroid hormone receptors, and reports about the relationship between the serum concentration of cortisol and the development of HCC are rare. The aim of this research was to establish a simple, specific, sensitive and reliable liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method for the quantitation of cortisol in human serum and to compare the level of...
Source: Biomedical Chromatography : BMC - February 14, 2024 Category: Biomedical Science Authors: Zijing Zhou Xiaoshuang Li Shengjun Yin Zhiyuan Zhang Xiaoyue Li Xiaojie Miao Haixu Cheng Yaqing Lou Pengfei Tu Yingyuan Lu Guoliang Zhang Source Type: research
