Development of a green high-performance liquid chromatography method for tofacitinib quantification in pharmaceutical formulations and degradation studies
Biomed Chromatogr. 2024 Apr 2:e5868. doi: 10.1002/bmc.5868. Online ahead of print.ABSTRACTA new high-performance liquid chromatography (HPLC) method was applied for the quantification of the active substance of tofacitinib. Analysis was performed on a Chromasil 100 C18 (100.0 × 4.0 mm, 3.5 μm) stationary phase. The mobile phase consisted of acetonitrile:0.2% phosphoric acid in water (12:88, v/v). The prepared sample (20.0 μL) was injected into the system. A detection wavelength of 285.0 nm was chosen for the compound, and the flow rate was 0.8 mL/min. The experiment was completed in 5.0 min. The analysis temperature was...
Source: Biomedical Chromatography : BMC - April 2, 2024 Category: Biomedical Science Authors: Gizem Tiris Asena Ayse Genc Nevin Erk Source Type: research
Development of a green high-performance liquid chromatography method for tofacitinib quantification in pharmaceutical formulations and degradation studies
Biomed Chromatogr. 2024 Apr 2:e5868. doi: 10.1002/bmc.5868. Online ahead of print.ABSTRACTA new high-performance liquid chromatography (HPLC) method was applied for the quantification of the active substance of tofacitinib. Analysis was performed on a Chromasil 100 C18 (100.0 × 4.0 mm, 3.5 μm) stationary phase. The mobile phase consisted of acetonitrile:0.2% phosphoric acid in water (12:88, v/v). The prepared sample (20.0 μL) was injected into the system. A detection wavelength of 285.0 nm was chosen for the compound, and the flow rate was 0.8 mL/min. The experiment was completed in 5.0 min. The analysis temperature was...
Source: Biomedical Chromatography : BMC - April 2, 2024 Category: Biomedical Science Authors: Gizem Tiris Asena Ayse Genc Nevin Erk Source Type: research
Development of a green high-performance liquid chromatography method for tofacitinib quantification in pharmaceutical formulations and degradation studies
Biomed Chromatogr. 2024 Apr 2:e5868. doi: 10.1002/bmc.5868. Online ahead of print.ABSTRACTA new high-performance liquid chromatography (HPLC) method was applied for the quantification of the active substance of tofacitinib. Analysis was performed on a Chromasil 100 C18 (100.0 × 4.0 mm, 3.5 μm) stationary phase. The mobile phase consisted of acetonitrile:0.2% phosphoric acid in water (12:88, v/v). The prepared sample (20.0 μL) was injected into the system. A detection wavelength of 285.0 nm was chosen for the compound, and the flow rate was 0.8 mL/min. The experiment was completed in 5.0 min. The analysis temperature was...
Source: Biomedical Chromatography : BMC - April 2, 2024 Category: Biomedical Science Authors: Gizem Tiris Asena Ayse Genc Nevin Erk Source Type: research
LC-MS/MS method for quick detection of vonoprazan fumarate in human plasma: Development, validation and its application to a bioequivalence study
Biomed Chromatogr. 2024 Apr 1:e5860. doi: 10.1002/bmc.5860. Online ahead of print.ABSTRACTA liquid chromatography-tandem mass spectrometry method with vonoprazan fumarate-d4 as a stable isotope-labeled internal standard was developed and validated aiming at quantification of vonoprazan fumarate in human plasma for a bioequivalence study. Chromatographic separation was achieved by acetonitrile one-step protein precipitation using a gradient elution of 0.1% formic acid aqueous solution and acetonitrile with a run time of 3.65 min. Detection was carried out on a tandem mass spectrometer in multiple reaction monitoring mode vi...
Source: Biomedical Chromatography : BMC - April 1, 2024 Category: Biomedical Science Authors: Xinyan Chen Tao Chen Yunzhe Huang Minhui Wang Yaqin Wang Ping Wu Xiaocui Xia Pengfei Du Jianbang Wu Jie Shen Yuanwei Jia Source Type: research
Evaluation of lipophilicity and drug-likeness of donepezil-like compounds using reversed-phase thin-layer chromatography
Biomed Chromatogr. 2024 Apr 1:e5867. doi: 10.1002/bmc.5867. Online ahead of print.ABSTRACTFourteen donepezil-like acetylcholinesterase (AChE) inhibitors from our library were analyzed using reversed-phase thin-layer chromatography to assess their lipophilicity and blood-brain barrier permeability. Compounds possessed N-benzylpiperidine and N,N-diarylpiperazine moieties connected via a short carboxamide or amine linker. Retention parameters RM 0, b, and C0 were considered as the measures of lipophilicity. Besides, logD of the investigated compounds was determined chromatographically using standard compounds with known logPo...
Source: Biomedical Chromatography : BMC - April 1, 2024 Category: Biomedical Science Authors: Sandra Šegan Mihajlo J Kruni ć Deana B Andri ć Vladimir B Šukalović Jelena Z Penji šević Ivana I Jevti ć Source Type: research
Olanzapine-induced weight gain and lipid dysfunction in mice between different gender
In this study, we found that olanzapine-treated mice showed varying degrees of dyslipidemia, which was particularly pronounced in female mice. Based on ultra-performance liquid chromatography-quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) technology and lipid metabolomics, we mapped the changes in lipid metabolism in olanzapine-treated mice and then compared the changes in lipid metabolism between male and female mice. There were 98 metabolic differentiators between the olanzapine-treated and control groups in females and 79 in males. These metabolites were glycerolipids, glycerophospholipids, fatty amides, and sphingolipids...
Source: Biomedical Chromatography : BMC - March 29, 2024 Category: Biomedical Science Authors: Tong Zhu Hongxia Zhao Yufan Chao Songyan Gao Xin Dong Zuowei Wang Source Type: research
Olanzapine-induced weight gain and lipid dysfunction in mice between different gender
In this study, we found that olanzapine-treated mice showed varying degrees of dyslipidemia, which was particularly pronounced in female mice. Based on ultra-performance liquid chromatography-quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) technology and lipid metabolomics, we mapped the changes in lipid metabolism in olanzapine-treated mice and then compared the changes in lipid metabolism between male and female mice. There were 98 metabolic differentiators between the olanzapine-treated and control groups in females and 79 in males. These metabolites were glycerolipids, glycerophospholipids, fatty amides, and sphingolipids...
Source: Biomedical Chromatography : BMC - March 29, 2024 Category: Biomedical Science Authors: Tong Zhu Hongxia Zhao Yufan Chao Songyan Gao Xin Dong Zuowei Wang Source Type: research
Olanzapine-induced weight gain and lipid dysfunction in mice between different gender
In this study, we found that olanzapine-treated mice showed varying degrees of dyslipidemia, which was particularly pronounced in female mice. Based on ultra-performance liquid chromatography-quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) technology and lipid metabolomics, we mapped the changes in lipid metabolism in olanzapine-treated mice and then compared the changes in lipid metabolism between male and female mice. There were 98 metabolic differentiators between the olanzapine-treated and control groups in females and 79 in males. These metabolites were glycerolipids, glycerophospholipids, fatty amides, and sphingolipids...
Source: Biomedical Chromatography : BMC - March 29, 2024 Category: Biomedical Science Authors: Tong Zhu Hongxia Zhao Yufan Chao Songyan Gao Xin Dong Zuowei Wang Source Type: research
Olanzapine-induced weight gain and lipid dysfunction in mice between different gender
In this study, we found that olanzapine-treated mice showed varying degrees of dyslipidemia, which was particularly pronounced in female mice. Based on ultra-performance liquid chromatography-quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) technology and lipid metabolomics, we mapped the changes in lipid metabolism in olanzapine-treated mice and then compared the changes in lipid metabolism between male and female mice. There were 98 metabolic differentiators between the olanzapine-treated and control groups in females and 79 in males. These metabolites were glycerolipids, glycerophospholipids, fatty amides, and sphingolipids...
Source: Biomedical Chromatography : BMC - March 29, 2024 Category: Biomedical Science Authors: Tong Zhu Hongxia Zhao Yufan Chao Songyan Gao Xin Dong Zuowei Wang Source Type: research
Unique biomedical application of fluorescence derivatization based on palladium-catalyzed coupling reactions for HPLC analysis of pharmaceuticals and biomolecules
Biomed Chromatogr. 2024 Mar 20:e5857. doi: 10.1002/bmc.5857. Online ahead of print.ABSTRACTPalladium-catalyzed coupling reactions are versatile and powerful tools for the construction of carbon-carbon bonds in organic synthesis. Although these reactions have favorable features that proceed selectively in mild reaction conditions using aqueous organic solvents, no attention has been given to their application in the field of biomedical analysis. Therefore, we focused on these reactions and evaluated the scope and limitations of their analytical performance. In this review, we describe the pros and cons and future trends of ...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Mahmoud El-Maghrabey Naoya Kishikawa Naotaka Kuroda Source Type: research
UPLC-Q-TOF/MS analysis of chemical components of single decoction and combined decoction of Qige decoction reveals the differences between various preparation processes
Biomed Chromatogr. 2024 Mar 21:e5865. doi: 10.1002/bmc.5865. Online ahead of print.ABSTRACTThe aim of this work was to explore the differences between various pharmaceutical processes in combined solutions of a single decoction (QGHBY) and a combined decoction (QGHJY) of Qi-Ge decoction from the perspective of chemical composition changes, so as to further guide the clinical application of drugs. A combined solution of a single decoction and a combined decoction of Astragali Radix, Puerariae Lobatae Radix and Citri Reticulatae Chachiensis Pericarpium was prepared with the same technological parameters. The chemical compone...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Kai-Xin Guo Xiao-Qing Yu Hui Tang Hao-Yang Wei Yan-Fang Li Si-Min Fan Ke-Er Huang Xue-Hong Ke Source Type: research
Unique biomedical application of fluorescence derivatization based on palladium-catalyzed coupling reactions for HPLC analysis of pharmaceuticals and biomolecules
Biomed Chromatogr. 2024 Mar 20:e5857. doi: 10.1002/bmc.5857. Online ahead of print.ABSTRACTPalladium-catalyzed coupling reactions are versatile and powerful tools for the construction of carbon-carbon bonds in organic synthesis. Although these reactions have favorable features that proceed selectively in mild reaction conditions using aqueous organic solvents, no attention has been given to their application in the field of biomedical analysis. Therefore, we focused on these reactions and evaluated the scope and limitations of their analytical performance. In this review, we describe the pros and cons and future trends of ...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Mahmoud El-Maghrabey Naoya Kishikawa Naotaka Kuroda Source Type: research
UPLC-Q-TOF/MS analysis of chemical components of single decoction and combined decoction of Qige decoction reveals the differences between various preparation processes
Biomed Chromatogr. 2024 Mar 21:e5865. doi: 10.1002/bmc.5865. Online ahead of print.ABSTRACTThe aim of this work was to explore the differences between various pharmaceutical processes in combined solutions of a single decoction (QGHBY) and a combined decoction (QGHJY) of Qi-Ge decoction from the perspective of chemical composition changes, so as to further guide the clinical application of drugs. A combined solution of a single decoction and a combined decoction of Astragali Radix, Puerariae Lobatae Radix and Citri Reticulatae Chachiensis Pericarpium was prepared with the same technological parameters. The chemical compone...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Kai-Xin Guo Xiao-Qing Yu Hui Tang Hao-Yang Wei Yan-Fang Li Si-Min Fan Ke-Er Huang Xue-Hong Ke Source Type: research
Unique biomedical application of fluorescence derivatization based on palladium-catalyzed coupling reactions for HPLC analysis of pharmaceuticals and biomolecules
Biomed Chromatogr. 2024 Mar 20:e5857. doi: 10.1002/bmc.5857. Online ahead of print.ABSTRACTPalladium-catalyzed coupling reactions are versatile and powerful tools for the construction of carbon-carbon bonds in organic synthesis. Although these reactions have favorable features that proceed selectively in mild reaction conditions using aqueous organic solvents, no attention has been given to their application in the field of biomedical analysis. Therefore, we focused on these reactions and evaluated the scope and limitations of their analytical performance. In this review, we describe the pros and cons and future trends of ...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Mahmoud El-Maghrabey Naoya Kishikawa Naotaka Kuroda Source Type: research
UPLC-Q-TOF/MS analysis of chemical components of single decoction and combined decoction of Qige decoction reveals the differences between various preparation processes
Biomed Chromatogr. 2024 Mar 21:e5865. doi: 10.1002/bmc.5865. Online ahead of print.ABSTRACTThe aim of this work was to explore the differences between various pharmaceutical processes in combined solutions of a single decoction (QGHBY) and a combined decoction (QGHJY) of Qi-Ge decoction from the perspective of chemical composition changes, so as to further guide the clinical application of drugs. A combined solution of a single decoction and a combined decoction of Astragali Radix, Puerariae Lobatae Radix and Citri Reticulatae Chachiensis Pericarpium was prepared with the same technological parameters. The chemical compone...
Source: Biomedical Chromatography : BMC - March 21, 2024 Category: Biomedical Science Authors: Kai-Xin Guo Xiao-Qing Yu Hui Tang Hao-Yang Wei Yan-Fang Li Si-Min Fan Ke-Er Huang Xue-Hong Ke Source Type: research
