A metabolism-based study of the mechanism of action of Scrophularia ningpoensis Hemsl. on nephrogenic edema
In this study, the main components and blood components of S. ningpoensis were identified using ultra-high-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS). Pathological section and blood biochemical analysis were used to estimate the therapeutic effect of S. ningpoensis on NE. Network pharmacology was used to predict the potential pathways of S. ningpoensis. The metabolomics method was used to study the changes in small-molecule metabolites in the body. The results showed that S. ningpoensis could relieve NE by regulating relative to renal function and body edema, and its mecha...
Source: Biomedical Chromatography : BMC - October 10, 2023 Category: Biomedical Science Authors: WanRu Dong BeiYu Xing JiaMin Yu Wei Li Yu Wang LuYang Zhong Ying Liu Yi Wang ZunPeng Shu ShuMin Liu Source Type: research
A metabolism-based study of the mechanism of action of Scrophularia ningpoensis Hemsl. on nephrogenic edema
In this study, the main components and blood components of S. ningpoensis were identified using ultra-high-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS). Pathological section and blood biochemical analysis were used to estimate the therapeutic effect of S. ningpoensis on NE. Network pharmacology was used to predict the potential pathways of S. ningpoensis. The metabolomics method was used to study the changes in small-molecule metabolites in the body. The results showed that S. ningpoensis could relieve NE by regulating relative to renal function and body edema, and its mecha...
Source: Biomedical Chromatography : BMC - October 10, 2023 Category: Biomedical Science Authors: WanRu Dong BeiYu Xing JiaMin Yu Wei Li Yu Wang LuYang Zhong Ying Liu Yi Wang ZunPeng Shu ShuMin Liu Source Type: research
Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring
This study aimed to develop a fast, accurate, and precise high-performance liquid chromatography with UV detection method for simultaneous analysis of underivatized phenylalanine (Phe) and tyrosine (Tyr) in biological samples. Separation of the analytes was accomplished using a Discovery HS F5-3 column, which offered better retention and peak symmetry for the tested analytes. Chromatographic conditions were optimized using central composite experimental design, and three factors were investigated: the concentration of ammonium acetate (A), the acetonitrile proportion in the mobile phase (B) and the column oven temperature ...
Source: Biomedical Chromatography : BMC - October 5, 2023 Category: Biomedical Science Authors: Hadjira Rabti Mounira Amrane Abdeldjalil Lalaouna Abdenacer Flilissa Yacine Benguerba Source Type: research
Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring
This study aimed to develop a fast, accurate, and precise high-performance liquid chromatography with UV detection method for simultaneous analysis of underivatized phenylalanine (Phe) and tyrosine (Tyr) in biological samples. Separation of the analytes was accomplished using a Discovery HS F5-3 column, which offered better retention and peak symmetry for the tested analytes. Chromatographic conditions were optimized using central composite experimental design, and three factors were investigated: the concentration of ammonium acetate (A), the acetonitrile proportion in the mobile phase (B) and the column oven temperature ...
Source: Biomedical Chromatography : BMC - October 5, 2023 Category: Biomedical Science Authors: Hadjira Rabti Mounira Amrane Abdeldjalil Lalaouna Abdenacer Flilissa Yacine Benguerba Source Type: research
Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring
This study aimed to develop a fast, accurate, and precise high-performance liquid chromatography with UV detection method for simultaneous analysis of underivatized phenylalanine (Phe) and tyrosine (Tyr) in biological samples. Separation of the analytes was accomplished using a Discovery HS F5-3 column, which offered better retention and peak symmetry for the tested analytes. Chromatographic conditions were optimized using central composite experimental design, and three factors were investigated: the concentration of ammonium acetate (A), the acetonitrile proportion in the mobile phase (B) and the column oven temperature ...
Source: Biomedical Chromatography : BMC - October 5, 2023 Category: Biomedical Science Authors: Hadjira Rabti Mounira Amrane Abdeldjalil Lalaouna Abdenacer Flilissa Yacine Benguerba Source Type: research
