CRISPR/Cas12-based electrochemical biosensors for clinical diagnostic and food monitoring
Bioelectrochemistry. 2023 Nov 5;155:108600. doi: 10.1016/j.bioelechem.2023.108600. Online ahead of print.ABSTRACTEach organism has a unique sequence of nitrogenous bases in in the form of DNA or RNA which distinguish them from other organisms. This characteristic makes nucleic acid-based detection extremely selective and compare to other molecular techniques. In recent years, several nucleic acid-based detection technology methods have been developed, one of which is the electrochemical biosensor. Electrochemical biosensors are known to have high sensitivity and accuracy. In addition, the ease of miniaturization of this el...
Source: Bioelectrochemistry - November 13, 2023 Category: Biochemistry Authors: Clianta Yudin Kharismasari None Irkham Muhammad Ihda H L Zein Ari Hardianto Salma Nur Zakiyyah Abdullahi Umar Ibrahim Mehmet Ozsoz Yeni Wahyuni Hartati Source Type: research
Clinical validation of electrochemical biosensor for the detection of methylglyoxal in subjects with type-2 diabetes mellitus
This study was designed to determine the utility of the developed electrochemical biosensor to measure the level of MG in human plasma from type-2 diabetes mellitus patients. Electrochemical studies were carried out with optimized experimental parameters using the modified Platinum-electrode. Subsequently, clinical studies using 350 blood plasma samples were conducted and the results were validated against the ELISA kit, Normal Glucose Tolerance (NGT), and glycosylated haemoglobin (HbA1c). The MG sensor exhibited a linear range of 1.0-7.5 μM concentration with a sensitivity of 1.02 mA µM-1, a limit of detection of 0.21 Â...
Source: Bioelectrochemistry - November 11, 2023 Category: Biochemistry Authors: Priyanga Kumar Noel Nesakumar Jayashree Gopal Sakthivel Sivasubramanian Srinivasan Vedantham John Bosco Balaguru Rayappan Source Type: research
Clinical validation of electrochemical biosensor for the detection of methylglyoxal in subjects with type-2 diabetes mellitus
This study was designed to determine the utility of the developed electrochemical biosensor to measure the level of MG in human plasma from type-2 diabetes mellitus patients. Electrochemical studies were carried out with optimized experimental parameters using the modified Platinum-electrode. Subsequently, clinical studies using 350 blood plasma samples were conducted and the results were validated against the ELISA kit, Normal Glucose Tolerance (NGT), and glycosylated haemoglobin (HbA1c). The MG sensor exhibited a linear range of 1.0-7.5 μM concentration with a sensitivity of 1.02 mA µM-1, a limit of detection of 0.21 Â...
Source: Bioelectrochemistry - November 11, 2023 Category: Biochemistry Authors: Priyanga Kumar Noel Nesakumar Jayashree Gopal Sakthivel Sivasubramanian Srinivasan Vedantham John Bosco Balaguru Rayappan Source Type: research
Proximity hybridization induced bipedal DNA walker and rolling circle amplification for label-free electrochemical detection of apolipoprotein A4
Bioelectrochemistry. 2023 Nov 2;155:108596. doi: 10.1016/j.bioelechem.2023.108596. Online ahead of print.ABSTRACTApolipoprotein A4 (Apo-A4) is considered as a prospective molecular biomarker for diagnosis of depression due to its neurosynaptic toxicity. We develop a proximity hybridization-induced DNAzyme-driven bipedal DNA walker strategy for Apo-A4 quantification based on rolling circle amplification (RCA) triggered by poly adenine binding to Ag nanoparticles (AgNPs). With the help of DNAzyme, the free-running bipedal DNA walker can quickly and sequentially shear a molecular beacon that acts as a primer to initiate the R...
Source: Bioelectrochemistry - November 8, 2023 Category: Biochemistry Authors: Chenyi Zhuo Dehong Yu Jiuying Cui Zichun Song Qianli Tang Xianjiu Liao Zhao Liu Ning Xin Lu Lou Fenglei Gao Source Type: research
Proximity hybridization induced bipedal DNA walker and rolling circle amplification for label-free electrochemical detection of apolipoprotein A4
Bioelectrochemistry. 2023 Nov 2;155:108596. doi: 10.1016/j.bioelechem.2023.108596. Online ahead of print.ABSTRACTApolipoprotein A4 (Apo-A4) is considered as a prospective molecular biomarker for diagnosis of depression due to its neurosynaptic toxicity. We develop a proximity hybridization-induced DNAzyme-driven bipedal DNA walker strategy for Apo-A4 quantification based on rolling circle amplification (RCA) triggered by poly adenine binding to Ag nanoparticles (AgNPs). With the help of DNAzyme, the free-running bipedal DNA walker can quickly and sequentially shear a molecular beacon that acts as a primer to initiate the R...
Source: Bioelectrochemistry - November 8, 2023 Category: Biochemistry Authors: Chenyi Zhuo Dehong Yu Jiuying Cui Zichun Song Qianli Tang Xianjiu Liao Zhao Liu Ning Xin Lu Lou Fenglei Gao Source Type: research
Proximity hybridization induced bipedal DNA walker and rolling circle amplification for label-free electrochemical detection of apolipoprotein A4
Bioelectrochemistry. 2023 Nov 2;155:108596. doi: 10.1016/j.bioelechem.2023.108596. Online ahead of print.ABSTRACTApolipoprotein A4 (Apo-A4) is considered as a prospective molecular biomarker for diagnosis of depression due to its neurosynaptic toxicity. We develop a proximity hybridization-induced DNAzyme-driven bipedal DNA walker strategy for Apo-A4 quantification based on rolling circle amplification (RCA) triggered by poly adenine binding to Ag nanoparticles (AgNPs). With the help of DNAzyme, the free-running bipedal DNA walker can quickly and sequentially shear a molecular beacon that acts as a primer to initiate the R...
Source: Bioelectrochemistry - November 8, 2023 Category: Biochemistry Authors: Chenyi Zhuo Dehong Yu Jiuying Cui Zichun Song Qianli Tang Xianjiu Liao Zhao Liu Ning Xin Lu Lou Fenglei Gao Source Type: research
Evaluation of a comprehensive power management system with maximum power point tracking algorithm for multiple microbial fuel cell energy harvesting
This study presents a comprehensive power management system (PMS) capable of tracking the maximum power point (MPP) and harvesting the energy from up to five microbial fuel cells (MFCs). The harvested energy from the MFCs was used to power the electronics, and in cases where this power was insufficient, alternative backup power options can be used. The voltage can be increased up to 3.3 V, and a hysteresis-based control approach was utilised to regulate the output voltage. The MPP of each MFC was determined using a variable step size incremental conductance algorithm that controls the duty cycle of the synchronous boost co...
Source: Bioelectrochemistry - November 6, 2023 Category: Biochemistry Authors: Rodrigo Fernandez Feito Tahreem Younas Richard M Dinsdale Source Type: research
Evaluation of a comprehensive power management system with maximum power point tracking algorithm for multiple microbial fuel cell energy harvesting
This study presents a comprehensive power management system (PMS) capable of tracking the maximum power point (MPP) and harvesting the energy from up to five microbial fuel cells (MFCs). The harvested energy from the MFCs was used to power the electronics, and in cases where this power was insufficient, alternative backup power options can be used. The voltage can be increased up to 3.3 V, and a hysteresis-based control approach was utilised to regulate the output voltage. The MPP of each MFC was determined using a variable step size incremental conductance algorithm that controls the duty cycle of the synchronous boost co...
Source: Bioelectrochemistry - November 6, 2023 Category: Biochemistry Authors: Rodrigo Fernandez Feito Tahreem Younas Richard M Dinsdale Source Type: research
Magnetically induced self-assembly electrochemical biosensor with ultra-low detection limit and extended measuring range for sensitive detection of HER2 protein
Bioelectrochemistry. 2023 Oct 25;155:108592. doi: 10.1016/j.bioelechem.2023.108592. Online ahead of print.ABSTRACTAn innovative electrochemical biosensor was fabricated for sensitive detection of human epidermal growth factor receptor 2 (HER2) protein, which was considered as an essential tumor marker for diagnosis and treatment evaluation of breast cancer. The sensor was constructed using Apt and PNA as recognition probes incorporated with magnetic Fe3O4/α-Fe2O3@Au nanocomposites. The sensing strategy was designed to lower the detection limit of HER2, and avoid the large steric interference caused by macromolecular HER2 ...
Source: Bioelectrochemistry - November 5, 2023 Category: Biochemistry Authors: Yanling Zhang Jie Wang Min Liu Yun Ni Yao Yue Dawei He Ruijiang Liu Source Type: research
Magnetically induced self-assembly electrochemical biosensor with ultra-low detection limit and extended measuring range for sensitive detection of HER2 protein
Bioelectrochemistry. 2023 Oct 25;155:108592. doi: 10.1016/j.bioelechem.2023.108592. Online ahead of print.ABSTRACTAn innovative electrochemical biosensor was fabricated for sensitive detection of human epidermal growth factor receptor 2 (HER2) protein, which was considered as an essential tumor marker for diagnosis and treatment evaluation of breast cancer. The sensor was constructed using Apt and PNA as recognition probes incorporated with magnetic Fe3O4/α-Fe2O3@Au nanocomposites. The sensing strategy was designed to lower the detection limit of HER2, and avoid the large steric interference caused by macromolecular HER2 ...
Source: Bioelectrochemistry - November 5, 2023 Category: Biochemistry Authors: Yanling Zhang Jie Wang Min Liu Yun Ni Yao Yue Dawei He Ruijiang Liu Source Type: research
Nitrogen-enhanced carbon quantum dots mediated immunosensor for electrochemical detection of HER2 breast cancer biomarker
Bioelectrochemistry. 2023 Oct 17;155:108589. doi: 10.1016/j.bioelechem.2023.108589. Online ahead of print.ABSTRACTUsing nitrogen-enhanced carbon quantum dots (N-CQDs) on a coated graphite sheet (GS) substrate (N-CQDs/GS), a simple strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was investigated. The bovine serum albumin (BSA)-modified HER2 Antibody/N-CQDs/GS immunoelectrode enabled excellent activity preservation for the biosensor, while the GS electrode provided a highly stable and conducting substrate. With a linear response range of 0.1 ng/mL-1 ng...
Source: Bioelectrochemistry - November 2, 2023 Category: Biochemistry Authors: Humayun Amir Vasanth Subramanian Sundaram Sornambikai Nagamony Ponpandian Chinnusamy Viswanathan Source Type: research
Nitrogen-enhanced carbon quantum dots mediated immunosensor for electrochemical detection of HER2 breast cancer biomarker
Bioelectrochemistry. 2023 Oct 17;155:108589. doi: 10.1016/j.bioelechem.2023.108589. Online ahead of print.ABSTRACTUsing nitrogen-enhanced carbon quantum dots (N-CQDs) on a coated graphite sheet (GS) substrate (N-CQDs/GS), a simple strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was investigated. The bovine serum albumin (BSA)-modified HER2 Antibody/N-CQDs/GS immunoelectrode enabled excellent activity preservation for the biosensor, while the GS electrode provided a highly stable and conducting substrate. With a linear response range of 0.1 ng/mL-1 ng...
Source: Bioelectrochemistry - November 2, 2023 Category: Biochemistry Authors: Humayun Amir Vasanth Subramanian Sundaram Sornambikai Nagamony Ponpandian Chinnusamy Viswanathan Source Type: research
Nitrogen-enhanced carbon quantum dots mediated immunosensor for electrochemical detection of HER2 breast cancer biomarker
Bioelectrochemistry. 2023 Oct 17;155:108589. doi: 10.1016/j.bioelechem.2023.108589. Online ahead of print.ABSTRACTUsing nitrogen-enhanced carbon quantum dots (N-CQDs) on a coated graphite sheet (GS) substrate (N-CQDs/GS), a simple strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was investigated. The bovine serum albumin (BSA)-modified HER2 Antibody/N-CQDs/GS immunoelectrode enabled excellent activity preservation for the biosensor, while the GS electrode provided a highly stable and conducting substrate. With a linear response range of 0.1 ng/mL-1 ng...
Source: Bioelectrochemistry - November 2, 2023 Category: Biochemistry Authors: Humayun Amir Vasanth Subramanian Sundaram Sornambikai Nagamony Ponpandian Chinnusamy Viswanathan Source Type: research
A graphitized carbon@boron carbide-mediated laccase-based amperometric biosensor for epinephrine detection
Bioelectrochemistry. 2023 Oct 21;155:108591. doi: 10.1016/j.bioelechem.2023.108591. Online ahead of print.ABSTRACTThe development of novel electron transfer mediator is important for laccase-based electrochemical biosensors. Here, graphitized carbon coated boron carbide (GC@B4C) was first used as the mediator to transfer electrons from laccase (Lac) to nickel foam (NF) electrode. The GC shell of GC@B4C particles was characterized and confirmed by HRTEM, XRD and Raman spectroscopy. The fabricated Lac/GC@B4C/NF electrode was applied for amperometric detection of epinephrine (EP). Detection results indicated Lac/GC@B4C/NF ele...
Source: Bioelectrochemistry - October 26, 2023 Category: Biochemistry Authors: Huanyu Tang Mingyue Cui Miaorong Zhang Yan Zhang Source Type: research
Electromechanical interactions between cell membrane and nuclear envelope: Beyond the standard Schwan's model of biological cells
Bioelectrochemistry. 2023 Oct 4;155:108583. doi: 10.1016/j.bioelechem.2023.108583. Online ahead of print.ABSTRACTWe investigate little-appreciated features of the hierarchical core-shell (CS) models of the electrical, mechanical, and electromechanical interactions between the cell membrane (CM) and nuclear envelope (NE). We first consider a simple model of an individual cell based on a coupled resistor-capacitor (Schwan model (SM)) network and show that the CM, when exposed to ac electric fields, acts as a low pass filter while the NE acts as a wide and asymmetric bandpass filter. We provide a simplified calculation for ch...
Source: Bioelectrochemistry - October 26, 2023 Category: Biochemistry Authors: Elias Sabri Christian Brosseau Source Type: research
