Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields
In this study, we explored cell death under conditions of reduced EFS and prolonged pulse application, identifying the contributions of electrolysis, reactive oxygen species (ROS) and ATP loss. We performed ablations with conventional high-voltage low pulse (HV-LP) and low-voltage high pulse (LV-HP) conditions in a 3D tumor mimic, finding equivalent ablation volumes when using 2000 V/cm 90 pulses or 1000 V/cm 900 pulses respectively. These results were confirmed by performing ablations in swine liver. In LV-HP treatment, ablation volume was found to increase proportionally with pulse numbers, without the substantial temper...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Neeraj Raghuraman Rajagopalan Tarek Munawar Mary Chase Sheehan Masashi Fujimori William-Ray Vista Thomas Wimmer Narendra Babu Gutta Stephen B Solomon Govindarajan Srimathveeravalli Source Type: research
Nanobody-guided redox and enzymatic functionalization of icosahedral virus particles for enhanced bioelectrocatalysis
Bioelectrochemistry. 2023 Sep 19;155:108570. doi: 10.1016/j.bioelechem.2023.108570. Online ahead of print.ABSTRACTIcosahedral, 30 nm diameter, grapevine fanleaf virus (GFLV) virus particles are adsorbed onto electrodes and used as nanoscaffolds for the assembly of an integrated glucose oxidizing system, comprising the enzyme pyrroloquinoline quinone-glucose dehydrogenase (PQQ-GDH) and ferrocenylated polyethylene glycol chains (Fc-PEG) as a redox co-substrate. Two different GFLV-specific nanobodies, either fused to the enzyme, or chemically conjugated to Fc-PEG, are used for the regio-selective immunodecoration of the viral...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Racha Kassem Anne Cousin Daniel Clesse Vianney Poignavent Adrien Trolet Christophe Ritzenthaler Thierry Michon Arnaud Chovin Christophe Demaille Source Type: research
Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields
In this study, we explored cell death under conditions of reduced EFS and prolonged pulse application, identifying the contributions of electrolysis, reactive oxygen species (ROS) and ATP loss. We performed ablations with conventional high-voltage low pulse (HV-LP) and low-voltage high pulse (LV-HP) conditions in a 3D tumor mimic, finding equivalent ablation volumes when using 2000 V/cm 90 pulses or 1000 V/cm 900 pulses respectively. These results were confirmed by performing ablations in swine liver. In LV-HP treatment, ablation volume was found to increase proportionally with pulse numbers, without the substantial temper...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Neeraj Raghuraman Rajagopalan Tarek Munawar Mary Chase Sheehan Masashi Fujimori William-Ray Vista Thomas Wimmer Narendra Babu Gutta Stephen B Solomon Govindarajan Srimathveeravalli Source Type: research
Nanobody-guided redox and enzymatic functionalization of icosahedral virus particles for enhanced bioelectrocatalysis
Bioelectrochemistry. 2023 Sep 19;155:108570. doi: 10.1016/j.bioelechem.2023.108570. Online ahead of print.ABSTRACTIcosahedral, 30 nm diameter, grapevine fanleaf virus (GFLV) virus particles are adsorbed onto electrodes and used as nanoscaffolds for the assembly of an integrated glucose oxidizing system, comprising the enzyme pyrroloquinoline quinone-glucose dehydrogenase (PQQ-GDH) and ferrocenylated polyethylene glycol chains (Fc-PEG) as a redox co-substrate. Two different GFLV-specific nanobodies, either fused to the enzyme, or chemically conjugated to Fc-PEG, are used for the regio-selective immunodecoration of the viral...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Racha Kassem Anne Cousin Daniel Clesse Vianney Poignavent Adrien Trolet Christophe Ritzenthaler Thierry Michon Arnaud Chovin Christophe Demaille Source Type: research
Intraoperative electrochemotherapy of the posterior resection surface after pancreaticoduodenectomy: Preliminary results of a hybrid approach treatment of pancreatic cancer
CONCLUSIONS: Our preliminary results suggest that a hybrid approach combining surgery with intraoperative electrochemotherapy is safe and feasible.PMID:37748261 | DOI:10.1016/j.bioelechem.2023.108576 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Žan Čebron Mihajlo Djoki ć Miha Petri č Maja Čemažar Ma ša Bošnjak Gregor Ser ša Bla ž Trotovšek Source Type: research
Unraveling the kinetics and pharmacology of human PepT1 using solid supported membrane-based electrophysiology
Bioelectrochemistry. 2023 Sep 16;155:108573. doi: 10.1016/j.bioelechem.2023.108573. Online ahead of print.ABSTRACTThe human Peptide Transporter 1 (hPepT1) is known for its broad substrate specificity and its ability to transport (pro-)drugs. Here, we present an in-depth comprehensive study of hPepT1 and its interactions with various substrates via solid supported membrane-based electrophysiology (SSME). Using hPepT1-containing vesicles, we could not identify any peptide induced pre-steady-state currents, indicating that the recorded peak currents reflect steady-state transport. Electrogenic co-transport of H+/glycylglycine...
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Alexander K örner Andre Bazzone Maximilian Wichert Maria Barthmes Srujan Kumar Dondapati Niels Fertig Stefan Kubick Source Type: research
Intraoperative electrochemotherapy of the posterior resection surface after pancreaticoduodenectomy: Preliminary results of a hybrid approach treatment of pancreatic cancer
CONCLUSIONS: Our preliminary results suggest that a hybrid approach combining surgery with intraoperative electrochemotherapy is safe and feasible.PMID:37748261 | DOI:10.1016/j.bioelechem.2023.108576 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Žan Čebron Mihajlo Djoki ć Miha Petri č Maja Čemažar Ma ša Bošnjak Gregor Ser ša Bla ž Trotovšek Source Type: research
Unraveling the kinetics and pharmacology of human PepT1 using solid supported membrane-based electrophysiology
Bioelectrochemistry. 2023 Sep 16;155:108573. doi: 10.1016/j.bioelechem.2023.108573. Online ahead of print.ABSTRACTThe human Peptide Transporter 1 (hPepT1) is known for its broad substrate specificity and its ability to transport (pro-)drugs. Here, we present an in-depth comprehensive study of hPepT1 and its interactions with various substrates via solid supported membrane-based electrophysiology (SSME). Using hPepT1-containing vesicles, we could not identify any peptide induced pre-steady-state currents, indicating that the recorded peak currents reflect steady-state transport. Electrogenic co-transport of H+/glycylglycine...
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Alexander K örner Andre Bazzone Maximilian Wichert Maria Barthmes Srujan Kumar Dondapati Niels Fertig Stefan Kubick Source Type: research
Intraoperative electrochemotherapy of the posterior resection surface after pancreaticoduodenectomy: Preliminary results of a hybrid approach treatment of pancreatic cancer
CONCLUSIONS: Our preliminary results suggest that a hybrid approach combining surgery with intraoperative electrochemotherapy is safe and feasible.PMID:37748261 | DOI:10.1016/j.bioelechem.2023.108576 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Žan Čebron Mihajlo Djoki ć Miha Petri č Maja Čemažar Ma ša Bošnjak Gregor Ser ša Bla ž Trotovšek Source Type: research
Unraveling the kinetics and pharmacology of human PepT1 using solid supported membrane-based electrophysiology
Bioelectrochemistry. 2023 Sep 16;155:108573. doi: 10.1016/j.bioelechem.2023.108573. Online ahead of print.ABSTRACTThe human Peptide Transporter 1 (hPepT1) is known for its broad substrate specificity and its ability to transport (pro-)drugs. Here, we present an in-depth comprehensive study of hPepT1 and its interactions with various substrates via solid supported membrane-based electrophysiology (SSME). Using hPepT1-containing vesicles, we could not identify any peptide induced pre-steady-state currents, indicating that the recorded peak currents reflect steady-state transport. Electrogenic co-transport of H+/glycylglycine...
Source: Bioelectrochemistry - September 25, 2023 Category: Biochemistry Authors: Alexander K örner Andre Bazzone Maximilian Wichert Maria Barthmes Srujan Kumar Dondapati Niels Fertig Stefan Kubick Source Type: research
Vitamin B < sub > 12 < /sub > -catalyzed electro-polymerization for ultrasensitive RNA detection
In this report, an electrochemical biosensor of the lung cancer biomarker microRNA-21 (miRNA-21) is designed for early screening of lung cancer with high sensitivity at the femtomolar level. In this approach, hairpin DNA with N3 end group was first attached to the electrode surface. When miRNA-21 was present and paired with hairpin DNA, the N3 group released and attached to the ATRP initiator through "click reaction". Through eATRP, a large number of FerrocenylMethyl Methacrylate (FcMMA) monomers polymerized into long chains for signal amplification. These long chains had a distinct electrical signal in the square wave vol...
Source: Bioelectrochemistry - September 24, 2023 Category: Biochemistry Authors: Yu Zhao Jian Zhang Nan Ma Jinming Kong Xueji Zhang Source Type: research
Vitamin B < sub > 12 < /sub > -catalyzed electro-polymerization for ultrasensitive RNA detection
In this report, an electrochemical biosensor of the lung cancer biomarker microRNA-21 (miRNA-21) is designed for early screening of lung cancer with high sensitivity at the femtomolar level. In this approach, hairpin DNA with N3 end group was first attached to the electrode surface. When miRNA-21 was present and paired with hairpin DNA, the N3 group released and attached to the ATRP initiator through "click reaction". Through eATRP, a large number of FerrocenylMethyl Methacrylate (FcMMA) monomers polymerized into long chains for signal amplification. These long chains had a distinct electrical signal in the square wave vol...
Source: Bioelectrochemistry - September 24, 2023 Category: Biochemistry Authors: Yu Zhao Jian Zhang Nan Ma Jinming Kong Xueji Zhang Source Type: research
Urban wastewater oxidation by bioelectrochemical systems: To what extent does the inoculum matter?
This study aims to assess the long-term influence of the inoculation strategy on real urban wastewater treatment by bioelectrochemical systems, focusing on both process performances and biofilm assembly dynamics. Four inoculation strategies were investigated in triplicates during six consecutive batches to treat primary clarifier effluent at lab scale. At the studied anodic potential (0.05 vs SHE), no long-term impact of the inoculation strategy on the performances was observed. Indeed, after three batches, electrochemical (88.0 ± 3.9 % coulombic efficiencies) and treatment performances (30.8 ± 3.9 % COD removals) conver...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Valentin Larzilli ère Florence de Fouch écour Chrystelle Bureau Th éodore Bouchez Roman Moscoviz Source Type: research
A voltammetric study of nitrogenase MoFe-protein using low-potential electron transfer mediators
Bioelectrochemistry. 2023 Sep 17;155:108575. doi: 10.1016/j.bioelechem.2023.108575. Online ahead of print.ABSTRACTThe molybdenum-iron protein (MoFeP), a component of the enzyme nitrogenase, catalyzes the reduction of an array of small molecules, including N2 to NH3. In microorganisms, during the catalytic cycle, MoFeP receives electrons from the obligate biological redox partner iron protein (FeP) in a process coupled to the hydrolysis of two MgATP per one electron transferred. Despite the favorable redox properties of the cofactors, the requirement of the MgATP hydrolysis significantly decreases the energy efficiency of M...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Artavazd Badalyan Zhi-Yong Yang Lance C Seefeldt Source Type: research
Nanosecond Pulsed Electric Fields (nsPEFs) Modulate Electron Transport in the Plasma Membrane and the Mitochondria
Bioelectrochemistry. 2023 Sep 9;155:108568. doi: 10.1016/j.bioelechem.2023.108568. Online ahead of print.ABSTRACTNanosecond pulsed electric fields (nsPEFs) are a pulsed power technology known for ablating tumors, but they also modulate diverse biological mechanisms. Here we show that nsPEFs regulate trans-plasma membrane electron transport (tPMET) rates in the plasma membrane redox system (PMRS) shown as a reduction of the cell-impermeable, WST-8 tetrazolium dye. At lower charging conditions, nsPEFs enhance, and at higher charging conditions inhibit tPMET in H9c2 non-cancerous cardiac myoblasts and 4T1-luc breast cancer ce...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Kamal Asadipour Maisoun Bani Hani Lucas Potter Brittney L Ruedlinger Nicola Lai Stephen J Beebe Source Type: research
