The comparison of the dynamics of Ca < sup > 2+ < /sup > and bleomycin intracellular delivery after cell sonoporation and electroporation in vitro
Bioelectrochemistry. 2024 Apr 14;158:108708. doi: 10.1016/j.bioelechem.2024.108708. Online ahead of print.ABSTRACTCa2+, in combination with SP or EP, induces cell cytotoxicity much faster compared to BLM. The application of BLM in combination with, SP or EP, reaches the level of cell death, induced by similar combination with Ca2+, only after 72 h. The methods of SP and EP were calibrated according to the level of differential cytotoxicity, determined after 6 days (using cell clonogenic assay). The combination of Ca2+ SP induces cell death faster than Ca2+ EP - after Ca2+ SP it increases to a maximum level after 15 min and...
Source: Bioelectrochemistry - April 18, 2024 Category: Biochemistry Authors: Martynas Maciulevi čius R ūta Palepšienė Salvijus Vykertas Renaldas Rai šutis Aras Rafanavi čius Tomas Krilavi čius Saulius Šatkauskas Source Type: research

Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research

Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research

Anaerobic treatment of groundwater co-contaminated by toluene and copper in a single chamber bioelectrochemical system
Bioelectrochemistry. 2024 Apr 13;158:108711. doi: 10.1016/j.bioelechem.2024.108711. Online ahead of print.ABSTRACTAddressing the simultaneous removal of multiple coexisting groundwater contaminants poses a significant challenge, primarily because of their different physicochemical properties. Indeed, different chemical compounds may necessitate establishing distinct, and sometimes conflicting, (bio)degradation and/or removal pathways. In this work, we investigated the concomitant anaerobic treatment of toluene and copper in a single-chamber bioelectrochemical cell with a potential difference of 1 V applied between the anod...
Source: Bioelectrochemistry - April 16, 2024 Category: Biochemistry Authors: Marco Resitano Matteo Tucci Alessio Mezzi Saulius Kaciulis Bruna Matturro Emilio D'Ugo Lucia Bertuccini Stefano Fazi Simona Rossetti Federico Aulenta Carolina Cruz Viggi Source Type: research

A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy
Bioelectrochemistry. 2024 Apr 13;158:108709. doi: 10.1016/j.bioelechem.2024.108709. Online ahead of print.ABSTRACTGlypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu2O) nanozyme was developed for GPC3 detection. The RGO-Cu2O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu2O oxidation fraction and the chronoamperometry (i-t) sig...
Source: Bioelectrochemistry - April 15, 2024 Category: Biochemistry Authors: Guiyin Li Huafu Feng Xinhao Li Shengnan Li Jintao Liang Zhide Zhou Source Type: research

A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy
Bioelectrochemistry. 2024 Apr 13;158:108709. doi: 10.1016/j.bioelechem.2024.108709. Online ahead of print.ABSTRACTGlypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu2O) nanozyme was developed for GPC3 detection. The RGO-Cu2O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu2O oxidation fraction and the chronoamperometry (i-t) sig...
Source: Bioelectrochemistry - April 15, 2024 Category: Biochemistry Authors: Guiyin Li Huafu Feng Xinhao Li Shengnan Li Jintao Liang Zhide Zhou Source Type: research

Optimised start-up strategy for bioelectrochemical systems operating on hydrolysed human urine
This study investigated a new strategy for bioanode inoculation by mixing real hydrolysed urine with thickened waste activated sludge (TWAS) from a municipal wastewater treatment plant at different volumetric ratios. The addition of TWAS diluted the high TAN concentration of hydrolysed urine (5.2 ± 0.3 g L-1) to 2.6-5.1 g L-1, while the pH of the inoculation mixtures remained > 9 and soluble chemical oxygen demand (sCOD) at 5.6-6.7 g L-1. Despite the high pH, current generation started within 24 h for all reactors, and robust bioanodes tolerant of continuous feeding with undiluted hydrolysed urine were enriched within ...
Source: Bioelectrochemistry - April 12, 2024 Category: Biochemistry Authors: Veera Koskue Stefano Freguia Source Type: research

Optimised start-up strategy for bioelectrochemical systems operating on hydrolysed human urine
This study investigated a new strategy for bioanode inoculation by mixing real hydrolysed urine with thickened waste activated sludge (TWAS) from a municipal wastewater treatment plant at different volumetric ratios. The addition of TWAS diluted the high TAN concentration of hydrolysed urine (5.2 ± 0.3 g L-1) to 2.6-5.1 g L-1, while the pH of the inoculation mixtures remained > 9 and soluble chemical oxygen demand (sCOD) at 5.6-6.7 g L-1. Despite the high pH, current generation started within 24 h for all reactors, and robust bioanodes tolerant of continuous feeding with undiluted hydrolysed urine were enriched within ...
Source: Bioelectrochemistry - April 12, 2024 Category: Biochemistry Authors: Veera Koskue Stefano Freguia Source Type: research

Effect of Halomonas titanicae on fluctuating water-line corrosion of EH40 steel
Bioelectrochemistry. 2024 Apr 4;158:108703. doi: 10.1016/j.bioelechem.2024.108703. Online ahead of print.ABSTRACTThe fluctuating water-line corrosion of EH40 steel in sterile and biotic media was investigated with a wire beam electrode. When the coupons were partially immersed in the sterile medium, the position of the low water-line acted as the cathodic zone and the area below the low water-line constantly served as the main anodic zone. The thin electrolyte layers with uneven thickness promoted the galvanic current of the region below the low water-line. Different from the sterile environment, the metabolism of Halomona...
Source: Bioelectrochemistry - April 10, 2024 Category: Biochemistry Authors: Ce Li Jiajia Wu Peng Wang Dun Zhang Liyang Zhu Yaohua Gao Wenkai Wang Hanzhe Sun Source Type: research

Effect of Halomonas titanicae on fluctuating water-line corrosion of EH40 steel
Bioelectrochemistry. 2024 Apr 4;158:108703. doi: 10.1016/j.bioelechem.2024.108703. Online ahead of print.ABSTRACTThe fluctuating water-line corrosion of EH40 steel in sterile and biotic media was investigated with a wire beam electrode. When the coupons were partially immersed in the sterile medium, the position of the low water-line acted as the cathodic zone and the area below the low water-line constantly served as the main anodic zone. The thin electrolyte layers with uneven thickness promoted the galvanic current of the region below the low water-line. Different from the sterile environment, the metabolism of Halomona...
Source: Bioelectrochemistry - April 10, 2024 Category: Biochemistry Authors: Ce Li Jiajia Wu Peng Wang Dun Zhang Liyang Zhu Yaohua Gao Wenkai Wang Hanzhe Sun Source Type: research

Effect of Halomonas titanicae on fluctuating water-line corrosion of EH40 steel
Bioelectrochemistry. 2024 Apr 4;158:108703. doi: 10.1016/j.bioelechem.2024.108703. Online ahead of print.ABSTRACTThe fluctuating water-line corrosion of EH40 steel in sterile and biotic media was investigated with a wire beam electrode. When the coupons were partially immersed in the sterile medium, the position of the low water-line acted as the cathodic zone and the area below the low water-line constantly served as the main anodic zone. The thin electrolyte layers with uneven thickness promoted the galvanic current of the region below the low water-line. Different from the sterile environment, the metabolism of Halomona...
Source: Bioelectrochemistry - April 10, 2024 Category: Biochemistry Authors: Ce Li Jiajia Wu Peng Wang Dun Zhang Liyang Zhu Yaohua Gao Wenkai Wang Hanzhe Sun Source Type: research

Point of care devices for detection of Covid-19, malaria and dengue infections: A review
Bioelectrochemistry. 2024 Apr 5;158:108704. doi: 10.1016/j.bioelechem.2024.108704. Online ahead of print.ABSTRACTNeed for affordable, rapid and user-friendly point of care (POC) devices are increasing exponentially for strengthening the health care system in primary care as well as for self- monitoring in routine analysis. In addition to routine analysis of glucose, Covid-19 type fast spreading, infectious diseases have created further push for exploring rapid, cost-effective and self-monitoring diagnostic devices. Successful implementation of self-monitoring devices for Covid -19 has been realized. However, not much succe...
Source: Bioelectrochemistry - April 9, 2024 Category: Biochemistry Authors: Susmita Baruah C A Betty Source Type: research

Point of care devices for detection of Covid-19, malaria and dengue infections: A review
Bioelectrochemistry. 2024 Apr 5;158:108704. doi: 10.1016/j.bioelechem.2024.108704. Online ahead of print.ABSTRACTNeed for affordable, rapid and user-friendly point of care (POC) devices are increasing exponentially for strengthening the health care system in primary care as well as for self- monitoring in routine analysis. In addition to routine analysis of glucose, Covid-19 type fast spreading, infectious diseases have created further push for exploring rapid, cost-effective and self-monitoring diagnostic devices. Successful implementation of self-monitoring devices for Covid -19 has been realized. However, not much succe...
Source: Bioelectrochemistry - April 9, 2024 Category: Biochemistry Authors: Susmita Baruah C A Betty Source Type: research

New insights into the mechanism of electrotransfer of small nucleic acids
Bioelectrochemistry. 2024 Apr 4;158:108696. doi: 10.1016/j.bioelechem.2024.108696. Online ahead of print.ABSTRACTRNA interference (RNAi) is a powerful and rapidly developing technology that enables precise silencing of genes of interest. However, the clinical development of RNAi is hampered by the limited cellular uptake and stability of the transferred molecules. Electroporation (EP) is an efficient and versatile technique for the transfer of both RNA and DNA. Although the mechanism of electrotransfer of small nucleic acids has been studied previously, too little is known about the potential effects of significantly large...
Source: Bioelectrochemistry - April 7, 2024 Category: Biochemistry Authors: R ūta Palepšienė Aswin Muralidharan Martynas Maciulevi čius Paulius Ruzgys Sonam Chopra Pouyan E Boukany Saulius Šatkauskas Source Type: research

New insights into the mechanism of electrotransfer of small nucleic acids
Bioelectrochemistry. 2024 Apr 4;158:108696. doi: 10.1016/j.bioelechem.2024.108696. Online ahead of print.ABSTRACTRNA interference (RNAi) is a powerful and rapidly developing technology that enables precise silencing of genes of interest. However, the clinical development of RNAi is hampered by the limited cellular uptake and stability of the transferred molecules. Electroporation (EP) is an efficient and versatile technique for the transfer of both RNA and DNA. Although the mechanism of electrotransfer of small nucleic acids has been studied previously, too little is known about the potential effects of significantly large...
Source: Bioelectrochemistry - April 7, 2024 Category: Biochemistry Authors: R ūta Palepšienė Aswin Muralidharan Martynas Maciulevi čius Paulius Ruzgys Sonam Chopra Pouyan E Boukany Saulius Šatkauskas Source Type: research