Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva
Bioelectrochemistry. 2023 Nov 29;156:108619. doi: 10.1016/j.bioelechem.2023.108619. Online ahead of print.ABSTRACTHerein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addi...
Source: Bioelectrochemistry - December 21, 2023 Category: Biochemistry Authors: Laura Fabiani Luca Fiore Silvia Fillo Nino D'Amore Riccardo De Santis Florigio Lista Fabiana Arduini Source Type: research
A signal amplification electrochemiluminescence biosensor based on Ru(bpy) < sub > 3 < /sub > < sup > 2+ < /sup > and β-cyclodextrin for detection of AFP
Bioelectrochemistry. 2023 Dec 14;156:108626. doi: 10.1016/j.bioelechem.2023.108626. Online ahead of print.ABSTRACTBy combining two different materials, metal-organic frameworks (MOF) and β-cyclodextrins (β-CD), a signal amplification electrochemical luminescence (ECL) immunosensor was constructed to realize the sensitive detection of AFP. The indium-based metal-organic framework (In-MOF) was used as the carrier of Ru(bpy)32+, and Ru(bpy)32+ was immobilized by In-MOF through suitable pore size and electrostatic interaction. At the same time, using host-guest recognition, β-CD enriched TPA into the hydrophobic cavity for ...
Source: Bioelectrochemistry - December 21, 2023 Category: Biochemistry Authors: Bing Cong Wenjin Liang Wenjing Lai Mingzhe Jiang Chaoyun Ma Chulei Zhao Wenwen Jiang Shaopeng Zhang Hongling Li Chenglin Hong Source Type: research
Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva
Bioelectrochemistry. 2023 Nov 29;156:108619. doi: 10.1016/j.bioelechem.2023.108619. Online ahead of print.ABSTRACTHerein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addi...
Source: Bioelectrochemistry - December 21, 2023 Category: Biochemistry Authors: Laura Fabiani Luca Fiore Silvia Fillo Nino D'Amore Riccardo De Santis Florigio Lista Fabiana Arduini Source Type: research
A signal amplification electrochemiluminescence biosensor based on Ru(bpy) < sub > 3 < /sub > < sup > 2+ < /sup > and β-cyclodextrin for detection of AFP
Bioelectrochemistry. 2023 Dec 14;156:108626. doi: 10.1016/j.bioelechem.2023.108626. Online ahead of print.ABSTRACTBy combining two different materials, metal-organic frameworks (MOF) and β-cyclodextrins (β-CD), a signal amplification electrochemical luminescence (ECL) immunosensor was constructed to realize the sensitive detection of AFP. The indium-based metal-organic framework (In-MOF) was used as the carrier of Ru(bpy)32+, and Ru(bpy)32+ was immobilized by In-MOF through suitable pore size and electrostatic interaction. At the same time, using host-guest recognition, β-CD enriched TPA into the hydrophobic cavity for ...
Source: Bioelectrochemistry - December 21, 2023 Category: Biochemistry Authors: Bing Cong Wenjin Liang Wenjing Lai Mingzhe Jiang Chaoyun Ma Chulei Zhao Wenwen Jiang Shaopeng Zhang Hongling Li Chenglin Hong Source Type: research
Flexible molecularly imprinted glucose sensor based on graphene sponge and Prussian blue
This study supports the development of wearable glucose sensors with high sensitivity, specificity, and stability through molecular imprinting.PMID:38104457 | DOI:10.1016/j.bioelechem.2023.108628 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Bin Li Yongqiang Dai Chaosheng Shi Xinying Guo Yizhong Chen Wei Zeng Source Type: research
Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement
This study shows the influence of the extracellular matrix on electric conductivity and electric field distribution on cell membrane electroporation and supports the adoption of more reliable 3D scaffolds in experimental electroporation studies.PMID:38104458 | DOI:10.1016/j.bioelechem.2023.108624 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Annj Zamuner Monica Dettin Luigi Dall'Olmo Luca Giovanni Campana Maria Evelina Mognaschi Maria Teresa Conconi Elisabetta Sieni Source Type: research
Flexible molecularly imprinted glucose sensor based on graphene sponge and Prussian blue
This study supports the development of wearable glucose sensors with high sensitivity, specificity, and stability through molecular imprinting.PMID:38104457 | DOI:10.1016/j.bioelechem.2023.108628 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Bin Li Yongqiang Dai Chaosheng Shi Xinying Guo Yizhong Chen Wei Zeng Source Type: research
Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement
This study shows the influence of the extracellular matrix on electric conductivity and electric field distribution on cell membrane electroporation and supports the adoption of more reliable 3D scaffolds in experimental electroporation studies.PMID:38104458 | DOI:10.1016/j.bioelechem.2023.108624 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Annj Zamuner Monica Dettin Luigi Dall'Olmo Luca Giovanni Campana Maria Evelina Mognaschi Maria Teresa Conconi Elisabetta Sieni Source Type: research
Flexible molecularly imprinted glucose sensor based on graphene sponge and Prussian blue
This study supports the development of wearable glucose sensors with high sensitivity, specificity, and stability through molecular imprinting.PMID:38104457 | DOI:10.1016/j.bioelechem.2023.108628 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Bin Li Yongqiang Dai Chaosheng Shi Xinying Guo Yizhong Chen Wei Zeng Source Type: research
Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement
This study shows the influence of the extracellular matrix on electric conductivity and electric field distribution on cell membrane electroporation and supports the adoption of more reliable 3D scaffolds in experimental electroporation studies.PMID:38104458 | DOI:10.1016/j.bioelechem.2023.108624 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Annj Zamuner Monica Dettin Luigi Dall'Olmo Luca Giovanni Campana Maria Evelina Mognaschi Maria Teresa Conconi Elisabetta Sieni Source Type: research
Flexible molecularly imprinted glucose sensor based on graphene sponge and Prussian blue
This study supports the development of wearable glucose sensors with high sensitivity, specificity, and stability through molecular imprinting.PMID:38104457 | DOI:10.1016/j.bioelechem.2023.108628 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Bin Li Yongqiang Dai Chaosheng Shi Xinying Guo Yizhong Chen Wei Zeng Source Type: research
Development of 3D melanoma cultures on a hyaluronic acid-based scaffold with synthetic self-assembling peptides: Electroporation enhancement
This study shows the influence of the extracellular matrix on electric conductivity and electric field distribution on cell membrane electroporation and supports the adoption of more reliable 3D scaffolds in experimental electroporation studies.PMID:38104458 | DOI:10.1016/j.bioelechem.2023.108624 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - December 17, 2023 Category: Biochemistry Authors: Annj Zamuner Monica Dettin Luigi Dall'Olmo Luca Giovanni Campana Maria Evelina Mognaschi Maria Teresa Conconi Elisabetta Sieni Source Type: research
Electrochemical assay of mammalian cell viability
We present the first use of amperometric detection to assess the viability of mammalian cells in continuous mode, directly in the cell culture medium. Vero or HeLa cells were injected into electrochemical sensors equipped with a 3-electrode system and containing DCIP 50 µM used as the redox mediator. DCIP was reduced by the viable cells and the reduced form was detected amperometrically at 300 mV vs silver pseudo-reference. The continuous regeneration of the oxidized form of the mediator ensured a stable redox state of the cell environment, allowing the cells to survive during the measurement time. The electrochemical res...
Source: Bioelectrochemistry - December 12, 2023 Category: Biochemistry Authors: Simon Guette-Marquet Valentin Saunier Ludovic Pilloux Christine Roques Alain Bergel Source Type: research
Electrochemical assay of mammalian cell viability
We present the first use of amperometric detection to assess the viability of mammalian cells in continuous mode, directly in the cell culture medium. Vero or HeLa cells were injected into electrochemical sensors equipped with a 3-electrode system and containing DCIP 50 µM used as the redox mediator. DCIP was reduced by the viable cells and the reduced form was detected amperometrically at 300 mV vs silver pseudo-reference. The continuous regeneration of the oxidized form of the mediator ensured a stable redox state of the cell environment, allowing the cells to survive during the measurement time. The electrochemical res...
Source: Bioelectrochemistry - December 12, 2023 Category: Biochemistry Authors: Simon Guette-Marquet Valentin Saunier Ludovic Pilloux Christine Roques Alain Bergel Source Type: research
Electrochemical assay of mammalian cell viability
We present the first use of amperometric detection to assess the viability of mammalian cells in continuous mode, directly in the cell culture medium. Vero or HeLa cells were injected into electrochemical sensors equipped with a 3-electrode system and containing DCIP 50 µM used as the redox mediator. DCIP was reduced by the viable cells and the reduced form was detected amperometrically at 300 mV vs silver pseudo-reference. The continuous regeneration of the oxidized form of the mediator ensured a stable redox state of the cell environment, allowing the cells to survive during the measurement time. The electrochemical res...
Source: Bioelectrochemistry - December 12, 2023 Category: Biochemistry Authors: Simon Guette-Marquet Valentin Saunier Ludovic Pilloux Christine Roques Alain Bergel Source Type: research
