A double methylene blue labeled single-stranded DNA and hairpin DNA coupling biosensor for the detection of Fusarium oxysporum f. sp. cubense race 4
Bioelectrochemistry. 2023 Nov 23;156:108612. doi: 10.1016/j.bioelechem.2023.108612. Online ahead of print.ABSTRACTThe DCL gene in Fusarium oxysporum f. sp. cubense Race 4 (Foc4) is a pivotal pathogenic factor causing banana fusarium wilt. Precise DCL detection is crucial for Foc4 containment. Here, we present a novel ssDNA-hDNA coupling electrochemical biosensor for highly specific DCL detection. The sensing interface was formed via electrodeposition of a composite containing reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) onto a carbon screen-printed electrode (SPE), followed by thiol-modified ssDNA functional...
Source: Bioelectrochemistry - November 30, 2023 Category: Biochemistry Authors: Zhihong Zhang Xiangying Ou Lizhe Ma Chunyu Li Zhou Yang Jieli Duan Source Type: research
Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayers
Bioelectrochemistry. 2023 Nov 22;156:108620. doi: 10.1016/j.bioelechem.2023.108620. Online ahead of print.ABSTRACTPathogen-triggered infections are the most severe global threat to human health, and to provide their timely treatment and prevention, robust methods for rapid and reliable identification of pathogenic microorganisms are required. Here, we have developed a fast and inexpensive electrocatalytic aptamer assay enabling specific and ultrasensitive detection of E. coli. E. coli, a biomarker of environmental contamination and infections, was captured on the mixed aptamer/thiolated PEG self-assembled monolayers formed...
Source: Bioelectrochemistry - November 25, 2023 Category: Biochemistry Authors: Rimsha B Jamal Ulrich Bay Gosewinkel Elena E Ferapontova Source Type: research
Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayers
Bioelectrochemistry. 2023 Nov 22;156:108620. doi: 10.1016/j.bioelechem.2023.108620. Online ahead of print.ABSTRACTPathogen-triggered infections are the most severe global threat to human health, and to provide their timely treatment and prevention, robust methods for rapid and reliable identification of pathogenic microorganisms are required. Here, we have developed a fast and inexpensive electrocatalytic aptamer assay enabling specific and ultrasensitive detection of E. coli. E. coli, a biomarker of environmental contamination and infections, was captured on the mixed aptamer/thiolated PEG self-assembled monolayers formed...
Source: Bioelectrochemistry - November 25, 2023 Category: Biochemistry Authors: Rimsha B Jamal Ulrich Bay Gosewinkel Elena E Ferapontova Source Type: research
Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayers
Bioelectrochemistry. 2023 Nov 22;156:108620. doi: 10.1016/j.bioelechem.2023.108620. Online ahead of print.ABSTRACTPathogen-triggered infections are the most severe global threat to human health, and to provide their timely treatment and prevention, robust methods for rapid and reliable identification of pathogenic microorganisms are required. Here, we have developed a fast and inexpensive electrocatalytic aptamer assay enabling specific and ultrasensitive detection of E. coli. E. coli, a biomarker of environmental contamination and infections, was captured on the mixed aptamer/thiolated PEG self-assembled monolayers formed...
Source: Bioelectrochemistry - November 25, 2023 Category: Biochemistry Authors: Rimsha B Jamal Ulrich Bay Gosewinkel Elena E Ferapontova Source Type: research
Electrocatalytic aptasensor for bacterial detection exploiting ferricyanide reduction by methylene blue on mixed PEG/aptamer monolayers
Bioelectrochemistry. 2023 Nov 22;156:108620. doi: 10.1016/j.bioelechem.2023.108620. Online ahead of print.ABSTRACTPathogen-triggered infections are the most severe global threat to human health, and to provide their timely treatment and prevention, robust methods for rapid and reliable identification of pathogenic microorganisms are required. Here, we have developed a fast and inexpensive electrocatalytic aptamer assay enabling specific and ultrasensitive detection of E. coli. E. coli, a biomarker of environmental contamination and infections, was captured on the mixed aptamer/thiolated PEG self-assembled monolayers formed...
Source: Bioelectrochemistry - November 25, 2023 Category: Biochemistry Authors: Rimsha B Jamal Ulrich Bay Gosewinkel Elena E Ferapontova Source Type: research
A ratiometric molecular imprinted electrochemiluminescence sensor based on enhanced luminescence of CdSe@ZnS quantum dots by MXene@NaAsc for detecting uric acid
Bioelectrochemistry. 2023 Nov 21;156:108610. doi: 10.1016/j.bioelechem.2023.108610. Online ahead of print.ABSTRACTAn unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid, based on MXene@NaAsc nanocomposites, CdSe@ZnS quantum dots and molecularly imprinted polymer composites modified glass carbon electrode. MXene@NaAsc stably enhanced the electron transfer and improved electrochemiluminescence intensity by acting as a base platform and signal amplifier for CdSe@ZnS quantum dots. Specific molecular imprinting cavities based on electropolymerization w...
Source: Bioelectrochemistry - November 24, 2023 Category: Biochemistry Authors: Miao Liu Yuwei Wang Shanshan Tang Wei Wang Axin Liang Aiqin Luo Source Type: research
A ratiometric molecular imprinted electrochemiluminescence sensor based on enhanced luminescence of CdSe@ZnS quantum dots by MXene@NaAsc for detecting uric acid
Bioelectrochemistry. 2023 Nov 21;156:108610. doi: 10.1016/j.bioelechem.2023.108610. Online ahead of print.ABSTRACTAn unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid, based on MXene@NaAsc nanocomposites, CdSe@ZnS quantum dots and molecularly imprinted polymer composites modified glass carbon electrode. MXene@NaAsc stably enhanced the electron transfer and improved electrochemiluminescence intensity by acting as a base platform and signal amplifier for CdSe@ZnS quantum dots. Specific molecular imprinting cavities based on electropolymerization w...
Source: Bioelectrochemistry - November 24, 2023 Category: Biochemistry Authors: Miao Liu Yuwei Wang Shanshan Tang Wei Wang Axin Liang Aiqin Luo Source Type: research
N-methyl mesoporphyrin IX (NMM) as electrochemical probe for detection of guanine quadruplexes
Bioelectrochemistry. 2023 Nov 17;156:108611. doi: 10.1016/j.bioelechem.2023.108611. Online ahead of print.ABSTRACTG-quadruplexes (G4) are stable alternative secondary structures of nucleic acids. With increasing understanding of their roles in biological processes and their application in bio- and nanotechnology, the exploration of novel methods for the analysis of these structures is becoming important. In this work, N-methyl mesoporphyrin IX (NMM) was used as a voltammetric probe for an easy electrochemical detection of G4s. Cyclic voltammetry on a hanging mercury drop electrode (HMDE) was used to detect NMM with a limit...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Daniel Dobrovodsky Ales Danhel Daniel Renciuk Jean-Louis Mergny Miroslav Fojta Source Type: research
Bacterial eradication by a low-energy pulsed electron beam generator
Bioelectrochemistry. 2023 Nov 10;156:108593. doi: 10.1016/j.bioelechem.2023.108593. Online ahead of print.ABSTRACTLow-energy electron beams (LEEB) are a safe and practical sterilization solution for in-line industrial applications, such as sterilizing medical products. However, their low dose rate induces product degradation, and the limited maximal energy prohibits high-throughput applications. To address this, we developed a low-energy 'pulsed' electron beam generator (LEPEB) and evaluated its efficacy and mechanism of action. Bacillus pumilus vegetative cells and spores were irradiated with a 250 keV LEPEB system at a 1...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Charlotte Da Silva Camille Lamarche Carole Pichereaux Emmanuelle Mouton-Barbosa Gauthier Demol S ébastien Boisne Etienne Dague Odile Burlet-Schiltz Flavien Pillet Marie-Pierre Rols Source Type: research
Sandwich-type immunosensor based on aminated 3D-rGOF-NH < sub > 2 < /sub > and CMK-3-Fc-MgAl-LDH multilayer nanocomposites for detection of CA125
In this study, we designed electrochemical sensors based on three-dimensional amino-functionalized reduced graphene oxide (3D-rGOF-NH2),2 MgAl layered double hydroxide nanocomposites containing ordered mesoporous carbon (CMK-3),3 and ferrocene carboxylic acids（Fc-COOH）4for the detection of CA125. 3D-rGOF-NH2 possesses good conductivity, a large surface area, and high porosity, enabling more immobilized nanoparticles to be deposited on its surface with excellent stability. CMK-3@Fc@MgAl-LDH nanocomposite was used as a carrier to enhance the immobilization of antibodies and the loading of Fc, conductors to enhance conduc...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Xinshui Ren Fangfang Wu Mengdie Wu Hongmin Gao Chunyan Wu Wendi Mu Simin Liu Longbin Que Hehua Zhang Meng Miao Dong Chang Hongzhi Pan Source Type: research
Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities
Bioelectrochemistry. 2023 Nov 20;156:108609. doi: 10.1016/j.bioelechem.2023.108609. Online ahead of print.ABSTRACTLignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it is therefore crucial to enhance or to identify new ligninolytic enzymes. Currently, the screening of ligninolytic activities on lignin polymers represents a technological bottenleck and hinders the characterization an...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Fall Issa Doum èche Bastien Abdellaoui Sofiene R émond Caroline Rakotoarivonina Harivony Ochs Marjorie Source Type: research
N-methyl mesoporphyrin IX (NMM) as electrochemical probe for detection of guanine quadruplexes
Bioelectrochemistry. 2023 Nov 17;156:108611. doi: 10.1016/j.bioelechem.2023.108611. Online ahead of print.ABSTRACTG-quadruplexes (G4) are stable alternative secondary structures of nucleic acids. With increasing understanding of their roles in biological processes and their application in bio- and nanotechnology, the exploration of novel methods for the analysis of these structures is becoming important. In this work, N-methyl mesoporphyrin IX (NMM) was used as a voltammetric probe for an easy electrochemical detection of G4s. Cyclic voltammetry on a hanging mercury drop electrode (HMDE) was used to detect NMM with a limit...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Daniel Dobrovodsky Ales Danhel Daniel Renciuk Jean-Louis Mergny Miroslav Fojta Source Type: research
Bacterial eradication by a low-energy pulsed electron beam generator
Bioelectrochemistry. 2023 Nov 10;156:108593. doi: 10.1016/j.bioelechem.2023.108593. Online ahead of print.ABSTRACTLow-energy electron beams (LEEB) are a safe and practical sterilization solution for in-line industrial applications, such as sterilizing medical products. However, their low dose rate induces product degradation, and the limited maximal energy prohibits high-throughput applications. To address this, we developed a low-energy 'pulsed' electron beam generator (LEPEB) and evaluated its efficacy and mechanism of action. Bacillus pumilus vegetative cells and spores were irradiated with a 250 keV LEPEB system at a 1...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Charlotte Da Silva Camille Lamarche Carole Pichereaux Emmanuelle Mouton-Barbosa Gauthier Demol S ébastien Boisne Etienne Dague Odile Burlet-Schiltz Flavien Pillet Marie-Pierre Rols Source Type: research
Sandwich-type immunosensor based on aminated 3D-rGOF-NH < sub > 2 < /sub > and CMK-3-Fc-MgAl-LDH multilayer nanocomposites for detection of CA125
In this study, we designed electrochemical sensors based on three-dimensional amino-functionalized reduced graphene oxide (3D-rGOF-NH2),2 MgAl layered double hydroxide nanocomposites containing ordered mesoporous carbon (CMK-3),3 and ferrocene carboxylic acids（Fc-COOH）4for the detection of CA125. 3D-rGOF-NH2 possesses good conductivity, a large surface area, and high porosity, enabling more immobilized nanoparticles to be deposited on its surface with excellent stability. CMK-3@Fc@MgAl-LDH nanocomposite was used as a carrier to enhance the immobilization of antibodies and the loading of Fc, conductors to enhance conduc...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Xinshui Ren Fangfang Wu Mengdie Wu Hongmin Gao Chunyan Wu Wendi Mu Simin Liu Longbin Que Hehua Zhang Meng Miao Dong Chang Hongzhi Pan Source Type: research
Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities
Bioelectrochemistry. 2023 Nov 20;156:108609. doi: 10.1016/j.bioelechem.2023.108609. Online ahead of print.ABSTRACTLignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it is therefore crucial to enhance or to identify new ligninolytic enzymes. Currently, the screening of ligninolytic activities on lignin polymers represents a technological bottenleck and hinders the characterization an...
Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Fall Issa Doum èche Bastien Abdellaoui Sofiene R émond Caroline Rakotoarivonina Harivony Ochs Marjorie Source Type: research
