Electrochemical assay of mammalian cell viability
We present the first use of amperometric detection to assess the viability of mammalian cells in continuous mode, directly in the cell culture medium. Vero or HeLa cells were injected into electrochemical sensors equipped with a 3-electrode system and containing DCIP 50 µM used as the redox mediator. DCIP was reduced by the viable cells and the reduced form was detected amperometrically at 300 mV vs silver pseudo-reference. The continuous regeneration of the oxidized form of the mediator ensured a stable redox state of the cell environment, allowing the cells to survive during the measurement time. The electrochemical res...
Source: Bioelectrochemistry - December 12, 2023 Category: Biochemistry Authors: Simon Guette-Marquet Valentin Saunier Ludovic Pilloux Christine Roques Alain Bergel Source Type: research
Response of current distribution in a liter-scale microbial fuel cell to variable operating conditions
In this study, the dynamic responses of current distribution to external stimuli (external load, temperature, pH, and electrolyte concentration) were investigated by employing a segmented anode current collector in a liter-scale MFC. The results demonstrated that, with respect to the anodic segment close to the cathode, a major response of the segment current to changes in load, temperature and pH was observed while minor response to changes in ion concentration. It was also found that external stimuli-induced high current usually led to a worse current distribution while increasing electrolyte ion concentration could simu...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Yudong Zhang Dong Li Liang Zhang Jun Li Qian Fu Xun Zhu Qiang Liao Source Type: research
Graphene transistor-based biosensors for rapid detection of SARS-CoV-2
Bioelectrochemistry. 2023 Dec 2;156:108623. doi: 10.1016/j.bioelechem.2023.108623. Online ahead of print.ABSTRACTField-effect transistor (FET) biosensors use FETs to detect changes in the amount of electrical charge caused by biomolecules like antigens and antibodies. COVID-19 can be detected by employing these biosensors by immobilising bio-receptor molecules that bind to the SARS-CoV-2 virus on the FET channel surface and subsequent monitoring of the changes in the current triggered by the virus. Graphene Field-effect Transistor (GFET)-based biosensors utilise graphene, a two-dimensional material with high electrical con...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Joydip Sengupta Chaudhery Mustansar Hussain Source Type: research
Response of current distribution in a liter-scale microbial fuel cell to variable operating conditions
In this study, the dynamic responses of current distribution to external stimuli (external load, temperature, pH, and electrolyte concentration) were investigated by employing a segmented anode current collector in a liter-scale MFC. The results demonstrated that, with respect to the anodic segment close to the cathode, a major response of the segment current to changes in load, temperature and pH was observed while minor response to changes in ion concentration. It was also found that external stimuli-induced high current usually led to a worse current distribution while increasing electrolyte ion concentration could simu...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Yudong Zhang Dong Li Liang Zhang Jun Li Qian Fu Xun Zhu Qiang Liao Source Type: research
Graphene transistor-based biosensors for rapid detection of SARS-CoV-2
Bioelectrochemistry. 2023 Dec 2;156:108623. doi: 10.1016/j.bioelechem.2023.108623. Online ahead of print.ABSTRACTField-effect transistor (FET) biosensors use FETs to detect changes in the amount of electrical charge caused by biomolecules like antigens and antibodies. COVID-19 can be detected by employing these biosensors by immobilising bio-receptor molecules that bind to the SARS-CoV-2 virus on the FET channel surface and subsequent monitoring of the changes in the current triggered by the virus. Graphene Field-effect Transistor (GFET)-based biosensors utilise graphene, a two-dimensional material with high electrical con...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Joydip Sengupta Chaudhery Mustansar Hussain Source Type: research
Response of current distribution in a liter-scale microbial fuel cell to variable operating conditions
In this study, the dynamic responses of current distribution to external stimuli (external load, temperature, pH, and electrolyte concentration) were investigated by employing a segmented anode current collector in a liter-scale MFC. The results demonstrated that, with respect to the anodic segment close to the cathode, a major response of the segment current to changes in load, temperature and pH was observed while minor response to changes in ion concentration. It was also found that external stimuli-induced high current usually led to a worse current distribution while increasing electrolyte ion concentration could simu...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Yudong Zhang Dong Li Liang Zhang Jun Li Qian Fu Xun Zhu Qiang Liao Source Type: research
Graphene transistor-based biosensors for rapid detection of SARS-CoV-2
Bioelectrochemistry. 2023 Dec 2;156:108623. doi: 10.1016/j.bioelechem.2023.108623. Online ahead of print.ABSTRACTField-effect transistor (FET) biosensors use FETs to detect changes in the amount of electrical charge caused by biomolecules like antigens and antibodies. COVID-19 can be detected by employing these biosensors by immobilising bio-receptor molecules that bind to the SARS-CoV-2 virus on the FET channel surface and subsequent monitoring of the changes in the current triggered by the virus. Graphene Field-effect Transistor (GFET)-based biosensors utilise graphene, a two-dimensional material with high electrical con...
Source: Bioelectrochemistry - December 9, 2023 Category: Biochemistry Authors: Joydip Sengupta Chaudhery Mustansar Hussain Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
Impact of temperature on the binding interaction between dsDNA and curcumin: An electrochemical study
In this study, we investigated the binding mode between double-stranded deoxyribonucleic acid (dsDNA) and curcumin (CU) using differential pulse voltammetry (DPV), UV-Vis spectroscopy, and molecular docking. By employing these techniques, we predicted the binding within the minor groove region of dsDNA and CU. Significantly, we employed electrochemistry, specifically cyclic voltammetry (CV), to explore the temperature effect on the dsDNA and CU binding. To the best of our knowledge, this is the first study to utilize electrochemical methods for investigating the temperature-dependent behavior of this binding interaction. O...
Source: Bioelectrochemistry - December 2, 2023 Category: Biochemistry Authors: Kimia Zarean Mousaabadi Ali A Ensafi Hassan Hadadzadeh Marziyeh Poshteh Shirani Source Type: research
A double methylene blue labeled single-stranded DNA and hairpin DNA coupling biosensor for the detection of Fusarium oxysporum f. sp. cubense race 4
Bioelectrochemistry. 2023 Nov 23;156:108612. doi: 10.1016/j.bioelechem.2023.108612. Online ahead of print.ABSTRACTThe DCL gene in Fusarium oxysporum f. sp. cubense Race 4 (Foc4) is a pivotal pathogenic factor causing banana fusarium wilt. Precise DCL detection is crucial for Foc4 containment. Here, we present a novel ssDNA-hDNA coupling electrochemical biosensor for highly specific DCL detection. The sensing interface was formed via electrodeposition of a composite containing reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) onto a carbon screen-printed electrode (SPE), followed by thiol-modified ssDNA functional...
Source: Bioelectrochemistry - November 30, 2023 Category: Biochemistry Authors: Zhihong Zhang Xiangying Ou Lizhe Ma Chunyu Li Zhou Yang Jieli Duan Source Type: research
A double methylene blue labeled single-stranded DNA and hairpin DNA coupling biosensor for the detection of Fusarium oxysporum f. sp. cubense race 4
Bioelectrochemistry. 2023 Nov 23;156:108612. doi: 10.1016/j.bioelechem.2023.108612. Online ahead of print.ABSTRACTThe DCL gene in Fusarium oxysporum f. sp. cubense Race 4 (Foc4) is a pivotal pathogenic factor causing banana fusarium wilt. Precise DCL detection is crucial for Foc4 containment. Here, we present a novel ssDNA-hDNA coupling electrochemical biosensor for highly specific DCL detection. The sensing interface was formed via electrodeposition of a composite containing reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) onto a carbon screen-printed electrode (SPE), followed by thiol-modified ssDNA functional...
Source: Bioelectrochemistry - November 30, 2023 Category: Biochemistry Authors: Zhihong Zhang Xiangying Ou Lizhe Ma Chunyu Li Zhou Yang Jieli Duan Source Type: research
