Calcium electroporation causes ATP depletion in cells and is effective both in microsecond and nanosecond pulse range as a modality of electrochemotherapy
Bioelectrochemistry. 2023 Sep 16;155:108574. doi: 10.1016/j.bioelechem.2023.108574. Online ahead of print.ABSTRACTCalcium electroporation is a modality of electrochemotherapy (ECT), which is based on intracellular electric field-mediated delivery of cytotoxic doses of calcium into the cells resulting in rapid cell death. In this work, we have developed a CHO-K1 luminescent cell line, which allowed the estimation of cell membrane permeabilization, ATP depletion and cytotoxicity evaluation without the use of additional markers and methodologies. We have shown the high efficiency of nanosecond pulses compressed into a MHz bur...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Eivina Radzevi čiūtė-Valčiukė Veronika Maly ško-Ptašinskė Egl ė Mickevičiūtė Julita Kulbacka Nina Rembia łkowska Auks ė Zinkevičienė Jurij Novickij Vitalij Novickij Source Type: research
Tetrahedral DNA nanostructure enhanced toehold-mediated strand displacement for highly sensitive electrochemiluminescence assay of CA125
Bioelectrochemistry. 2023 Sep 16;155:108572. doi: 10.1016/j.bioelechem.2023.108572. Online ahead of print.ABSTRACTCancer antigen 125 (CA125) is a typical tumor marker of ovarian cancer. Here, a multi-amplified electrochemiluminescence (ECL) aptasensor was developed for efficient recognition of CA125 using tetrahedral DNA nanostructure (TDN) enhanced toehold-mediated strand displacement (TMSD) coupled with gold nanoparticles/Ru(bpy)32+/metal-organic framework (AuNPs/Ru/ZIF-MOF) signal probe. AuNPs and Ru(bpy)32+ modified ZIF-MOF acted as initial ECL signal and further used for the immobilization of TDN, the activated DNA te...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Weiwei Zhang Wenwen Wang Yueyue Yu Source Type: research
Urban wastewater oxidation by bioelectrochemical systems: To what extent does the inoculum matter?
This study aims to assess the long-term influence of the inoculation strategy on real urban wastewater treatment by bioelectrochemical systems, focusing on both process performances and biofilm assembly dynamics. Four inoculation strategies were investigated in triplicates during six consecutive batches to treat primary clarifier effluent at lab scale. At the studied anodic potential (0.05 vs SHE), no long-term impact of the inoculation strategy on the performances was observed. Indeed, after three batches, electrochemical (88.0 ± 3.9 % coulombic efficiencies) and treatment performances (30.8 ± 3.9 % COD removals) conver...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Valentin Larzilli ère Florence de Fouch écour Chrystelle Bureau Th éodore Bouchez Roman Moscoviz Source Type: research
A voltammetric study of nitrogenase MoFe-protein using low-potential electron transfer mediators
Bioelectrochemistry. 2023 Sep 17;155:108575. doi: 10.1016/j.bioelechem.2023.108575. Online ahead of print.ABSTRACTThe molybdenum-iron protein (MoFeP), a component of the enzyme nitrogenase, catalyzes the reduction of an array of small molecules, including N2 to NH3. In microorganisms, during the catalytic cycle, MoFeP receives electrons from the obligate biological redox partner iron protein (FeP) in a process coupled to the hydrolysis of two MgATP per one electron transferred. Despite the favorable redox properties of the cofactors, the requirement of the MgATP hydrolysis significantly decreases the energy efficiency of M...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Artavazd Badalyan Zhi-Yong Yang Lance C Seefeldt Source Type: research
Nanosecond Pulsed Electric Fields (nsPEFs) Modulate Electron Transport in the Plasma Membrane and the Mitochondria
Bioelectrochemistry. 2023 Sep 9;155:108568. doi: 10.1016/j.bioelechem.2023.108568. Online ahead of print.ABSTRACTNanosecond pulsed electric fields (nsPEFs) are a pulsed power technology known for ablating tumors, but they also modulate diverse biological mechanisms. Here we show that nsPEFs regulate trans-plasma membrane electron transport (tPMET) rates in the plasma membrane redox system (PMRS) shown as a reduction of the cell-impermeable, WST-8 tetrazolium dye. At lower charging conditions, nsPEFs enhance, and at higher charging conditions inhibit tPMET in H9c2 non-cancerous cardiac myoblasts and 4T1-luc breast cancer ce...
Source: Bioelectrochemistry - September 22, 2023 Category: Biochemistry Authors: Kamal Asadipour Maisoun Bani Hani Lucas Potter Brittney L Ruedlinger Nicola Lai Stephen J Beebe Source Type: research
