An aptamerelectrochemical sensor based on functional carbon nanofibers for tetracycline determination
Bioelectrochemistry. 2024 Feb 10;157:108668. doi: 10.1016/j.bioelechem.2024.108668. Online ahead of print.ABSTRACTFe-Co@CNF was synthesized by electrospinning technology, and AuNPs was loaded onto Fe-Co@CNF by in-situ reduction to obtain Fe-Co@CNF@AuNPs composite material, which was used as the working electrode based on Au-S bond cooperation. The tetracycline electrochemical sensing interface Fe-Co@CNF@AuNPs@Apt was constructed by connecting mercaptoylated tetracycline (TC) aptamers on Fe-Co@CNF@AuNPs surface. The morphology and composition of Fe-Co@CNF@AuNPs composites were characterized by SEM, TEM, EDS, XRD and XPS, an...
Source: Bioelectrochemistry - February 22, 2024 Category: Biochemistry Authors: Li Zhang Ming Yin Xiuxia Wei Yiwei Sun Yuting Luo Huaqing Lin Ruxin Shu Dongpo Xu Source Type: research
An aptamerelectrochemical sensor based on functional carbon nanofibers for tetracycline determination
Bioelectrochemistry. 2024 Feb 10;157:108668. doi: 10.1016/j.bioelechem.2024.108668. Online ahead of print.ABSTRACTFe-Co@CNF was synthesized by electrospinning technology, and AuNPs was loaded onto Fe-Co@CNF by in-situ reduction to obtain Fe-Co@CNF@AuNPs composite material, which was used as the working electrode based on Au-S bond cooperation. The tetracycline electrochemical sensing interface Fe-Co@CNF@AuNPs@Apt was constructed by connecting mercaptoylated tetracycline (TC) aptamers on Fe-Co@CNF@AuNPs surface. The morphology and composition of Fe-Co@CNF@AuNPs composites were characterized by SEM, TEM, EDS, XRD and XPS, an...
Source: Bioelectrochemistry - February 22, 2024 Category: Biochemistry Authors: Li Zhang Ming Yin Xiuxia Wei Yiwei Sun Yuting Luo Huaqing Lin Ruxin Shu Dongpo Xu Source Type: research
An aptamerelectrochemical sensor based on functional carbon nanofibers for tetracycline determination
Bioelectrochemistry. 2024 Feb 10;157:108668. doi: 10.1016/j.bioelechem.2024.108668. Online ahead of print.ABSTRACTFe-Co@CNF was synthesized by electrospinning technology, and AuNPs was loaded onto Fe-Co@CNF by in-situ reduction to obtain Fe-Co@CNF@AuNPs composite material, which was used as the working electrode based on Au-S bond cooperation. The tetracycline electrochemical sensing interface Fe-Co@CNF@AuNPs@Apt was constructed by connecting mercaptoylated tetracycline (TC) aptamers on Fe-Co@CNF@AuNPs surface. The morphology and composition of Fe-Co@CNF@AuNPs composites were characterized by SEM, TEM, EDS, XRD and XPS, an...
Source: Bioelectrochemistry - February 22, 2024 Category: Biochemistry Authors: Li Zhang Ming Yin Xiuxia Wei Yiwei Sun Yuting Luo Huaqing Lin Ruxin Shu Dongpo Xu Source Type: research
Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells
In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal disti...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: Josie L Duncan Raffae N Ahmad Hunter Danesi Daniel J Slade Rafael V Davalos Scott S Verbridge Source Type: research
Quantitative determination of dopamine in the presence of interfering substances supported by machine learning tools
Bioelectrochemistry. 2024 Feb 10;157:108667. doi: 10.1016/j.bioelechem.2024.108667. Online ahead of print.ABSTRACTIn the field of neuroscience as well as in the clinical setting, the neurotransmitter dopamine (DA) is an analyte which is important for research as well as medical purposes. There are plenty of methods available to measure dopamine quantitatively, with voltammetric ones such as differential pulse voltammetry (DPV) being among the most convenient and simple ones. However, dopamine often occurs, either naturally or because of the requirements of involved enzymatic systems, alongside substances that can influence...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: C Kallabis P Beyerlein F Lisdat Source Type: research
Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells
In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal disti...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: Josie L Duncan Raffae N Ahmad Hunter Danesi Daniel J Slade Rafael V Davalos Scott S Verbridge Source Type: research
Quantitative determination of dopamine in the presence of interfering substances supported by machine learning tools
Bioelectrochemistry. 2024 Feb 10;157:108667. doi: 10.1016/j.bioelechem.2024.108667. Online ahead of print.ABSTRACTIn the field of neuroscience as well as in the clinical setting, the neurotransmitter dopamine (DA) is an analyte which is important for research as well as medical purposes. There are plenty of methods available to measure dopamine quantitatively, with voltammetric ones such as differential pulse voltammetry (DPV) being among the most convenient and simple ones. However, dopamine often occurs, either naturally or because of the requirements of involved enzymatic systems, alongside substances that can influence...
Source: Bioelectrochemistry - February 20, 2024 Category: Biochemistry Authors: C Kallabis P Beyerlein F Lisdat Source Type: research
Corrigendum to "A microfabricated lab-on-chip with three-dimensional electrodes for microscopic observation of bioelectromagnetic effects of cells" [Bioelectrochemistry 154 (2023) 108554]
Bioelectrochemistry. 2024 Feb 17:108641. doi: 10.1016/j.bioelechem.2024.108641. Online ahead of print.NO ABSTRACTPMID:38369444 | DOI:10.1016/j.bioelechem.2024.108641 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - February 18, 2024 Category: Biochemistry Authors: Yingxian Lu Yigong Shi Source Type: research
Corrigendum to "A microfabricated lab-on-chip with three-dimensional electrodes for microscopic observation of bioelectromagnetic effects of cells" [Bioelectrochemistry 154 (2023) 108554]
Bioelectrochemistry. 2024 Feb 17:108641. doi: 10.1016/j.bioelechem.2024.108641. Online ahead of print.NO ABSTRACTPMID:38369444 | DOI:10.1016/j.bioelechem.2024.108641 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - February 18, 2024 Category: Biochemistry Authors: Yingxian Lu Yigong Shi Source Type: research
Corrigendum to "A microfabricated lab-on-chip with three-dimensional electrodes for microscopic observation of bioelectromagnetic effects of cells" [Bioelectrochemistry 154 (2023) 108554]
Bioelectrochemistry. 2024 Feb 17:108641. doi: 10.1016/j.bioelechem.2024.108641. Online ahead of print.NO ABSTRACTPMID:38369444 | DOI:10.1016/j.bioelechem.2024.108641 (Source: Bioelectrochemistry)
Source: Bioelectrochemistry - February 18, 2024 Category: Biochemistry Authors: Yingxian Lu Yigong Shi Source Type: research
Fibroblasts transfection by electroporation in 3D reconstructed human dermal tissue
Bioelectrochemistry. 2024 Feb 13;157:108670. doi: 10.1016/j.bioelechem.2024.108670. Online ahead of print.ABSTRACTThe understanding of the mechanisms involved in DNA electrotransfer in human skin remains modest and limits the clinical development of various biomedical applications, such as DNA vaccination. To elucidate some mechanisms of DNA transfer in the skin following electroporation, we created a model of the dermis using a tissue engineering approach. This model allowed us to study the electrotransfection of fibroblasts in a three-dimensional environment that included multiple layers of fibroblasts as well as the sel...
Source: Bioelectrochemistry - February 16, 2024 Category: Biochemistry Authors: G éraldine Albérola Elisabeth Bellard Jelena Kolosnjaj-Tabi Jorgan Guard Muriel Golzio Marie-Pierre Rols Source Type: research
Fibroblasts transfection by electroporation in 3D reconstructed human dermal tissue
Bioelectrochemistry. 2024 Feb 13;157:108670. doi: 10.1016/j.bioelechem.2024.108670. Online ahead of print.ABSTRACTThe understanding of the mechanisms involved in DNA electrotransfer in human skin remains modest and limits the clinical development of various biomedical applications, such as DNA vaccination. To elucidate some mechanisms of DNA transfer in the skin following electroporation, we created a model of the dermis using a tissue engineering approach. This model allowed us to study the electrotransfection of fibroblasts in a three-dimensional environment that included multiple layers of fibroblasts as well as the sel...
Source: Bioelectrochemistry - February 16, 2024 Category: Biochemistry Authors: G éraldine Albérola Elisabeth Bellard Jelena Kolosnjaj-Tabi Jorgan Guard Muriel Golzio Marie-Pierre Rols Source Type: research
Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes
Bioelectrochemistry. 2024 Feb 10;157:108663. doi: 10.1016/j.bioelechem.2024.108663. Online ahead of print.ABSTRACTA new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7 mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were teste...
Source: Bioelectrochemistry - February 15, 2024 Category: Biochemistry Authors: Bohdan Josypcuk Sofiia Tvorynska Source Type: research
Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes
Bioelectrochemistry. 2024 Feb 10;157:108663. doi: 10.1016/j.bioelechem.2024.108663. Online ahead of print.ABSTRACTA new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7 mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were teste...
Source: Bioelectrochemistry - February 15, 2024 Category: Biochemistry Authors: Bohdan Josypcuk Sofiia Tvorynska Source Type: research
Bioelectrochemical cascade reaction for energy-saving hydrogen production and innovative Zn-air batteries
Bioelectrochemistry. 2024 Feb 10;157:108666. doi: 10.1016/j.bioelechem.2024.108666. Online ahead of print.ABSTRACTThe oxygen evolution reaction (OER) is an important half-reaction in electrochemical hydrogen production (EHP) and rechargeable metal-air batteries. However, the sluggish OER kinetics has seriously impeded their performance. Herein, we report a bioelectrochemical cascade system composed of glucose oxidase (GOx)-functionalized N-doped porous carbon nanofibers to replace OER in EHP and rechargeable Zn-air batteries (ZABs) applications. In this cascade system, GOx catalyzes oxidation of glucose to produce value-ad...
Source: Bioelectrochemistry - February 12, 2024 Category: Biochemistry Authors: Yuxia Zhang Yan Zheng Hongfen Deng Yating Long Wenna Jiang Chen Li Siping Li Zhi Li Gangyong Li Source Type: research
