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Introducing common oxazine fluorophores as new redox labels for electrochemical DNA sensors
Bioelectrochemistry. 2023 Sep 30;155:108582. doi: 10.1016/j.bioelechem.2023.108582. Online ahead of print.ABSTRACTThe electrochemical properties of three oxazine fluorophores, ATTO655, ATTO680 and ATTO700 have been investigated at gold electrodes. They display a reversible or quasi-reversible voltammetric behaviour involving either a 2e-, 2H+ or a 2e-, 1H+ redox process depending on the pH, at a formal potential located in the stability range of thiolate self-assembled monolayers (E°' ≈ -0.33 V vs. Ag|AgCl|3M KCl at pH 7.2). The performance of ATTO655 as redox label for electrochemical nucleic acid sensing was evaluated...
Source: Bioelectrochemistry - October 8, 2023 Category: Biochemistry Authors: Milutin Smiljanic Pierre Bleteau Alexia Papageorgiou Nathan Goffart Catherine Adam Thomas Doneux Source Type: research

Characterizing reversible, irreversible, and calcium electroporation to generate a burst-dependent dynamic conductivity curve
Bioelectrochemistry. 2023 Sep 25;155:108580. doi: 10.1016/j.bioelechem.2023.108580. Online ahead of print.ABSTRACTThe relationships between burst number, reversible, irreversible, and calcium electroporation have not been comprehensively evaluated in tumor tissue-mimics. Our findings indicate that electroporation effects saturate with a rate constant (τ) of 20 bursts for both conventional and high frequency waveforms (R2 > 0.88), with the separation between reversible and irreversible electroporation thresholds converging at 50 bursts. We find the lethal thresholds for calcium electroporation are statistically similar ...
Source: Bioelectrochemistry - October 3, 2023 Category: Biochemistry Authors: Edward J Jacobs Iv Sabrina N Campelo Alyssa Charlton Sara Altreuter Rafael V Davalos Source Type: research

Kinetic modeling of 2e - /1H + and 2e - /2H + bidirectional catalytic cycles
Bioelectrochemistry. 2023 Sep 21;155:108511. doi: 10.1016/j.bioelechem.2023.108511. Online ahead of print.ABSTRACTWhen a redox enzyme or synthetic catalyst is interfaced with an electrode, the electrochemical response depends on the details of the catalytic cycle. Here we focus on the steady-state catalytic waveshape of enzymes such as formate dehydrogenase (2e-/1H+), hydrogenases (2e-/2H+) and other bidirectional molecular catalysts that can be adsorbed on, and undergo direct electron transfer with an electrode. We seek to examine the relations between the dependence on pH of the waveshape, the sequence of events in the c...
Source: Bioelectrochemistry - October 2, 2023 Category: Biochemistry Authors: Andrea Fasano Vincent Fourmond Christophe L éger Source Type: research

Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields
In this study, we explored cell death under conditions of reduced EFS and prolonged pulse application, identifying the contributions of electrolysis, reactive oxygen species (ROS) and ATP loss. We performed ablations with conventional high-voltage low pulse (HV-LP) and low-voltage high pulse (LV-HP) conditions in a 3D tumor mimic, finding equivalent ablation volumes when using 2000 V/cm 90 pulses or 1000 V/cm 900 pulses respectively. These results were confirmed by performing ablations in swine liver. In LV-HP treatment, ablation volume was found to increase proportionally with pulse numbers, without the substantial temper...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Neeraj Raghuraman Rajagopalan Tarek Munawar Mary Chase Sheehan Masashi Fujimori William-Ray Vista Thomas Wimmer Narendra Babu Gutta Stephen B Solomon Govindarajan Srimathveeravalli Source Type: research

Nanobody-guided redox and enzymatic functionalization of icosahedral virus particles for enhanced bioelectrocatalysis
Bioelectrochemistry. 2023 Sep 19;155:108570. doi: 10.1016/j.bioelechem.2023.108570. Online ahead of print.ABSTRACTIcosahedral, 30 nm diameter, grapevine fanleaf virus (GFLV) virus particles are adsorbed onto electrodes and used as nanoscaffolds for the assembly of an integrated glucose oxidizing system, comprising the enzyme pyrroloquinoline quinone-glucose dehydrogenase (PQQ-GDH) and ferrocenylated polyethylene glycol chains (Fc-PEG) as a redox co-substrate. Two different GFLV-specific nanobodies, either fused to the enzyme, or chemically conjugated to Fc-PEG, are used for the regio-selective immunodecoration of the viral...
Source: Bioelectrochemistry - September 28, 2023 Category: Biochemistry Authors: Racha Kassem Anne Cousin Daniel Clesse Vianney Poignavent Adrien Trolet Christophe Ritzenthaler Thierry Michon Arnaud Chovin Christophe Demaille Source Type: research