Magnetic self-assembled label-free electrochemical biosensor based on Fe < sub > 3 < /sub > O < sub > 4 < /sub > / α-Fe < sub > 2 < /sub > O < sub > 3 < /sub > heterogeneous nanosheets for the detection of Tau proteins
Bioelectrochemistry. 2024 Mar 6;157:108678. doi: 10.1016/j.bioelechem.2024.108678. Online ahead of print.ABSTRACTA type of electrochemical biosensors based on magnetic Fe3O4/α-Fe2O3 heterogeneous nanosheets was constructed to detect Tau proteins for early diagnosis and intervention therapy of Alzheimer's disease (AD). Firstly, Fe3O4/α-Fe2O3 heterogeneous nanosheets were fabricated as the substrate to realize magnetic self-assembly and magnetic separation to improve current response, and Fe3O4/α-Fe2O3@Au-Apt/ssDNA/MCH biosensors were successfully constructed through the reduction process of chloroauric acid, the immobili...
Source: Bioelectrochemistry - March 7, 2024 Category: Biochemistry Authors: Jie Wang Hezhong Ouyang Yun Ni Haoda Zhang Lei Sun Ruijiang Liu Shasha Li Source Type: research
Magnetic self-assembled label-free electrochemical biosensor based on Fe < sub > 3 < /sub > O < sub > 4 < /sub > / α-Fe < sub > 2 < /sub > O < sub > 3 < /sub > heterogeneous nanosheets for the detection of Tau proteins
Bioelectrochemistry. 2024 Mar 6;157:108678. doi: 10.1016/j.bioelechem.2024.108678. Online ahead of print.ABSTRACTA type of electrochemical biosensors based on magnetic Fe3O4/α-Fe2O3 heterogeneous nanosheets was constructed to detect Tau proteins for early diagnosis and intervention therapy of Alzheimer's disease (AD). Firstly, Fe3O4/α-Fe2O3 heterogeneous nanosheets were fabricated as the substrate to realize magnetic self-assembly and magnetic separation to improve current response, and Fe3O4/α-Fe2O3@Au-Apt/ssDNA/MCH biosensors were successfully constructed through the reduction process of chloroauric acid, the immobili...
Source: Bioelectrochemistry - March 7, 2024 Category: Biochemistry Authors: Jie Wang Hezhong Ouyang Yun Ni Haoda Zhang Lei Sun Ruijiang Liu Shasha Li Source Type: research
Determination of carcinoembryonic antigen (CEA) by label-free electrochemical immunosensor using functionalized boron nitride nanosheets
In this study, a simple, specific and sensitive immunosensor for CEA detection was prepared based on BN nanosheets. Lewis acid-base interaction was sufficient for the immobilization of anti-CEA used as an antibody on the electrode surface without an activation agent. This immunosensor could be used for CEA determination without the need to use any label or secondary antibody. With its epedance-based recognition mechanism, this immunosensor offered a low LOD value of 0.017 ng/mL and a wide measurement range of 0.1-500 ng/mL and could be used for a long time. The analytical performance of this immunosensor is higher than the...
Source: Bioelectrochemistry - March 3, 2024 Category: Biochemistry Authors: Baha Öndeş Ulviye Kilimci Murat Uygun Deniz Akta ş Uygun Source Type: research
Determination of carcinoembryonic antigen (CEA) by label-free electrochemical immunosensor using functionalized boron nitride nanosheets
In this study, a simple, specific and sensitive immunosensor for CEA detection was prepared based on BN nanosheets. Lewis acid-base interaction was sufficient for the immobilization of anti-CEA used as an antibody on the electrode surface without an activation agent. This immunosensor could be used for CEA determination without the need to use any label or secondary antibody. With its epedance-based recognition mechanism, this immunosensor offered a low LOD value of 0.017 ng/mL and a wide measurement range of 0.1-500 ng/mL and could be used for a long time. The analytical performance of this immunosensor is higher than the...
Source: Bioelectrochemistry - March 3, 2024 Category: Biochemistry Authors: Baha Öndeş Ulviye Kilimci Murat Uygun Deniz Akta ş Uygun Source Type: research
Determination of carcinoembryonic antigen (CEA) by label-free electrochemical immunosensor using functionalized boron nitride nanosheets
In this study, a simple, specific and sensitive immunosensor for CEA detection was prepared based on BN nanosheets. Lewis acid-base interaction was sufficient for the immobilization of anti-CEA used as an antibody on the electrode surface without an activation agent. This immunosensor could be used for CEA determination without the need to use any label or secondary antibody. With its epedance-based recognition mechanism, this immunosensor offered a low LOD value of 0.017 ng/mL and a wide measurement range of 0.1-500 ng/mL and could be used for a long time. The analytical performance of this immunosensor is higher than the...
Source: Bioelectrochemistry - March 3, 2024 Category: Biochemistry Authors: Baha Öndeş Ulviye Kilimci Murat Uygun Deniz Akta ş Uygun Source Type: research
Determination of carcinoembryonic antigen (CEA) by label-free electrochemical immunosensor using functionalized boron nitride nanosheets
In this study, a simple, specific and sensitive immunosensor for CEA detection was prepared based on BN nanosheets. Lewis acid-base interaction was sufficient for the immobilization of anti-CEA used as an antibody on the electrode surface without an activation agent. This immunosensor could be used for CEA determination without the need to use any label or secondary antibody. With its epedance-based recognition mechanism, this immunosensor offered a low LOD value of 0.017 ng/mL and a wide measurement range of 0.1-500 ng/mL and could be used for a long time. The analytical performance of this immunosensor is higher than the...
Source: Bioelectrochemistry - March 3, 2024 Category: Biochemistry Authors: Baha Öndeş Ulviye Kilimci Murat Uygun Deniz Akta ş Uygun Source Type: research
A sensitive immunosensing platform based on the high cathodic photoelectrochemical activity of Zr-MOF and dual-signal amplification of peroxidase-mimetic Fe-MOF
Bioelectrochemistry. 2024 Feb 29;157:108677. doi: 10.1016/j.bioelechem.2024.108677. Online ahead of print.ABSTRACTCathodic photoelectrochemical (PEC) analysis has received special concerns because of its outstanding anti-interference capability toward reductive substances in samples, so it is highly desirable to develop high-performance photocathodic materials for PEC analysis. Herein, a Zr-based metal-organic framework (Zr-MOF), MOF-525, is explored as a photoactive material in aqueous solution for the first time, which shows a narrow band-gap of 1.82 eV, excellent visible-light absorption, and high cathodic PEC activity....
Source: Bioelectrochemistry - March 2, 2024 Category: Biochemistry Authors: Zhong Cheng Guihua He Rong Liao Yueming Tan Wenfang Deng Source Type: research
Photo-sensitive peptide inducing targeted cross-linking in a one-step and reagent-, enzyme- and antibody-free detection of SARS-Cov-2 marker protein
Bioelectrochemistry. 2024 Feb 25;157:108672. doi: 10.1016/j.bioelechem.2024.108672. Online ahead of print.ABSTRACTModern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing s...
Source: Bioelectrochemistry - March 1, 2024 Category: Biochemistry Authors: Meihui Hou Wenmin Hou Mingyu Qin Qun Wang Lei Zhou Source Type: research
Photo-sensitive peptide inducing targeted cross-linking in a one-step and reagent-, enzyme- and antibody-free detection of SARS-Cov-2 marker protein
Bioelectrochemistry. 2024 Feb 25;157:108672. doi: 10.1016/j.bioelechem.2024.108672. Online ahead of print.ABSTRACTModern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing s...
Source: Bioelectrochemistry - March 1, 2024 Category: Biochemistry Authors: Meihui Hou Wenmin Hou Mingyu Qin Qun Wang Lei Zhou Source Type: research
The organo-metal-like nature of long-range conduction in cable bacteria
Bioelectrochemistry. 2024 Feb 25;157:108675. doi: 10.1016/j.bioelechem.2024.108675. Online ahead of print.ABSTRACTCable bacteria are filamentous, multicellular microorganisms that display an exceptional form of biological electron transport across centimeter-scale distances. Currents are guided through a network of nickel-containing protein fibers within the cell envelope. Still, the mechanism of long-range conduction remains unresolved. Here, we characterize the conductance of the fiber network under dry and wet, physiologically relevant, conditions. Our data reveal that the fiber conductivity is high (median value: 27 S ...
Source: Bioelectrochemistry - February 29, 2024 Category: Biochemistry Authors: Dmitrii Pankratov Silvia Hidalgo Martinez Cheryl Karman Anastasia Gerzhik Gabriel Gomila Stanislav Trashin Henricus T S Boschker Jeanine S Geelhoed Dirk Mayer Karolien De Wael Filip J R Meysman Source Type: research
The organo-metal-like nature of long-range conduction in cable bacteria
Bioelectrochemistry. 2024 Feb 25;157:108675. doi: 10.1016/j.bioelechem.2024.108675. Online ahead of print.ABSTRACTCable bacteria are filamentous, multicellular microorganisms that display an exceptional form of biological electron transport across centimeter-scale distances. Currents are guided through a network of nickel-containing protein fibers within the cell envelope. Still, the mechanism of long-range conduction remains unresolved. Here, we characterize the conductance of the fiber network under dry and wet, physiologically relevant, conditions. Our data reveal that the fiber conductivity is high (median value: 27 S ...
Source: Bioelectrochemistry - February 29, 2024 Category: Biochemistry Authors: Dmitrii Pankratov Silvia Hidalgo Martinez Cheryl Karman Anastasia Gerzhik Gabriel Gomila Stanislav Trashin Henricus T S Boschker Jeanine S Geelhoed Dirk Mayer Karolien De Wael Filip J R Meysman Source Type: research
Fabrication of an affordable and sensitive corticosteroid-binding globulin immunosensor based on electrodeposited gold nanoparticles modified glassy carbon electrode
Bioelectrochemistry. 2024 Feb 22;157:108671. doi: 10.1016/j.bioelechem.2024.108671. Online ahead of print.ABSTRACTHerein, we fabricated an ultrasensitive electrochemical immunosensor for the quantitative detection of corticosteroid-binding globulin (CBG). CBG is a protein that regulates glucocorticoid levels and is an important biomarker for inflammation. A decrease in CBG levels is a key biomarker for inflammatory diseases, such as septic shock. To enhance the electrochemical performance and provide a large surface area for anti-CBG immobilization, we functionalized the glassy carbon electrode surface with AuNPs. Electroc...
Source: Bioelectrochemistry - February 24, 2024 Category: Biochemistry Authors: Gulam Rabbani Abrar Ahmad Mazin A Zamzami Othman A Baothman Salman A Hosawi Hisham Altayeb Muhammad Shahid Nadeem Varish Ahmad Source Type: research
Fabrication of an affordable and sensitive corticosteroid-binding globulin immunosensor based on electrodeposited gold nanoparticles modified glassy carbon electrode
Bioelectrochemistry. 2024 Feb 22;157:108671. doi: 10.1016/j.bioelechem.2024.108671. Online ahead of print.ABSTRACTHerein, we fabricated an ultrasensitive electrochemical immunosensor for the quantitative detection of corticosteroid-binding globulin (CBG). CBG is a protein that regulates glucocorticoid levels and is an important biomarker for inflammation. A decrease in CBG levels is a key biomarker for inflammatory diseases, such as septic shock. To enhance the electrochemical performance and provide a large surface area for anti-CBG immobilization, we functionalized the glassy carbon electrode surface with AuNPs. Electroc...
Source: Bioelectrochemistry - February 24, 2024 Category: Biochemistry Authors: Gulam Rabbani Abrar Ahmad Mazin A Zamzami Othman A Baothman Salman A Hosawi Hisham Altayeb Muhammad Shahid Nadeem Varish Ahmad Source Type: research
Fabrication of an affordable and sensitive corticosteroid-binding globulin immunosensor based on electrodeposited gold nanoparticles modified glassy carbon electrode
Bioelectrochemistry. 2024 Feb 22;157:108671. doi: 10.1016/j.bioelechem.2024.108671. Online ahead of print.ABSTRACTHerein, we fabricated an ultrasensitive electrochemical immunosensor for the quantitative detection of corticosteroid-binding globulin (CBG). CBG is a protein that regulates glucocorticoid levels and is an important biomarker for inflammation. A decrease in CBG levels is a key biomarker for inflammatory diseases, such as septic shock. To enhance the electrochemical performance and provide a large surface area for anti-CBG immobilization, we functionalized the glassy carbon electrode surface with AuNPs. Electroc...
Source: Bioelectrochemistry - February 24, 2024 Category: Biochemistry Authors: Gulam Rabbani Abrar Ahmad Mazin A Zamzami Othman A Baothman Salman A Hosawi Hisham Altayeb Muhammad Shahid Nadeem Varish Ahmad Source Type: research
Fabrication of an affordable and sensitive corticosteroid-binding globulin immunosensor based on electrodeposited gold nanoparticles modified glassy carbon electrode
Bioelectrochemistry. 2024 Feb 22;157:108671. doi: 10.1016/j.bioelechem.2024.108671. Online ahead of print.ABSTRACTHerein, we fabricated an ultrasensitive electrochemical immunosensor for the quantitative detection of corticosteroid-binding globulin (CBG). CBG is a protein that regulates glucocorticoid levels and is an important biomarker for inflammation. A decrease in CBG levels is a key biomarker for inflammatory diseases, such as septic shock. To enhance the electrochemical performance and provide a large surface area for anti-CBG immobilization, we functionalized the glassy carbon electrode surface with AuNPs. Electroc...
Source: Bioelectrochemistry - February 24, 2024 Category: Biochemistry Authors: Gulam Rabbani Abrar Ahmad Mazin A Zamzami Othman A Baothman Salman A Hosawi Hisham Altayeb Muhammad Shahid Nadeem Varish Ahmad Source Type: research
