Paper-based electrodes as a tool for detecting ligninolytic enzymatic activities

Bioelectrochemistry. 2023 Nov 20;156:108609. doi: 10.1016/j.bioelechem.2023.108609. Online ahead of print.ABSTRACTLignin is the most important natural source of aromatic compounds. The valorisation of lignin into aromatics requires fractionation steps that can be catalysed by ligninolytic enzymes. However, one of the main limitations of biological lignin fractionation is the low efficiency of biocatalysts; it is therefore crucial to enhance or to identify new ligninolytic enzymes. Currently, the screening of ligninolytic activities on lignin polymers represents a technological bottenleck and hinders the characterization and the discovery of efficient ligninolytic biocatalysts. An efficient and fast method for the measurement of such enzymatic activities is therefore required. In this work, we present a new electrochemical tool based on lignin-coated paper electrodes for the detection and the characterization of ligninolytic activity. The suitability of this method is demonstrated using a catalase-peroxidase isolated from Thermobacillus xylanilyticus.PMID:37995505 | DOI:10.1016/j.bioelechem.2023.108609

Source: Bioelectrochemistry - November 23, 2023 Category: Biochemistry Authors: Fall Issa Doum èche Bastien Abdellaoui Sofiene R émond Caroline Rakotoarivonina Harivony Ochs Marjorie Source Type: research