Biochemical and Biophysical Research communications This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Influence of storage solution, temperature, assay time and concentration on RT-qPCR nucleic acid detection for SARS-CoV-2 detection of SARS-CoV-2 by the RT-qPCR
In conclusion, storage temperature, storage time and storage status (inactivated, non-inactivated) have no effect on the nucleic acid detection of SARS-CoV-2 at the same concentration. For different concentrations of SARS-CoV-2, the detection of N gene is mainly affected.PMID:38493747 | DOI:10.1016/j.bbrc.2024.149726 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - March 17, 2024 Category: Biochemistry Authors: Lingxuan Su Yihan Lou Jiaxuan Li Haiyan Mao Jianhua Li Yi Sun Biaofeng Zhou Guangshang Wu Chen Huang Yanjun Zhang Keda Chen Source Type: research

Systemic loss of CD36 aggravates NAFLD-related HCC through MEK1/2-ERK1/2 signaling pathway
CONCLUSION: Systemic loss of CD36 leaded to the advancement of NAFLD to HCC by causing lipid disorders and metabolic inflammation, a process that involves the activation of MAPK signaling pathway. We found that CD36 contributes significantly to the maintenance of metabolic homeostasis in NAFLD-HCC.PMID:38492244 | DOI:10.1016/j.bbrc.2024.149781 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - March 16, 2024 Category: Biochemistry Authors: Enze Zheng Qianqian Chen Anhua Xiao Xiaoqing Luo Qiannan Lu Chuan Tian Huan Liu Jinqing Zhao Li Wei Ping Yang Yaxi Chen Source Type: research

Mismatch repair enzymes regulate telomere recombination in Saccharomycescerevisiae
This study investigates the participation of MMR in telomere recombination. Our findings reveal that mutations in MMR genes activate type I recombination. Notably, among the MMR proteins, MutSα (Msh2 and Msh6) and MutLα (Mlh1 and Pms1) exerted the most pronounced effects on telomere recombination. We also found that yeast cells containing simple human telomeric TTAGGG DNA sequences preferentially utilize type II recombination to maintain their telomeres, highlighting the influence of the heterogeneous nature of yeast telomeric sequences on type II recombination. Furthermore, our observations indicate that MMR activity is...
Source: Biochemical and Biophysical Research communications - March 15, 2024 Category: Biochemistry Authors: Chia-Chun Liu Mathilde M M Capart Jing-Jer Lin Source Type: research

Role of transcriptional cofactors in cardiovascular diseases
Biochem Biophys Res Commun. 2024 Mar 11;706:149757. doi: 10.1016/j.bbrc.2024.149757. Online ahead of print.ABSTRACTCardiovascular disease is a main cause of mortality in the world and the highest incidence of all diseases. However, the mechanism of the pathogenesis of cardiovascular disease is still unclear, and we need to continue to explore its mechanism of action. The occurrence and development of cardiovascular disease is significantly associated with genetic abnormalities, and gene expression is affected by transcriptional regulation. In this complex process, the protein-protein interaction promotes the RNA polymerase...
Source: Biochemical and Biophysical Research communications - March 15, 2024 Category: Biochemistry Authors: Shuqing Mao Chao Song Hong Huang Yali Nie Kai Ding Jian Cui Jinwei Tian Huifang Tang Source Type: research

Is myeloid-derived growth factor a ligand of the sphingosine-1-phosphate receptor 2?
Biochem Biophys Res Commun. 2024 Mar 10;706:149766. doi: 10.1016/j.bbrc.2024.149766. Online ahead of print.ABSTRACTSecretory myeloid-derived growth factor (MYDGF) exerts beneficial effects on organ repair, probably via a plasma membrane receptor; however, the identity of the expected receptor has remained elusive. In a recent study, MYDGF was reported as an agonist of the sphingosine-1-phosphate receptor 2 (S1PR2), an A-class G protein-coupled receptor that mediates the functions of the signaling lipid, sphingosine-1-phosphate (S1P). In the present study, we conducted living cell-based functional assays to test whether S1P...
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Yong-Shan Zheng Ya-Li Liu Zeng-Guang Xu Cheng He Zhan-Yun Guo Source Type: research

Light-dependent expression and accumulation of miR408-encoded peptide, miPEP408, is regulated by HY5 in Arabidopsis
This study underscores the regulatory impact of light on small peptides, exemplified by miPEP408, mediated by the key transcription factor HY5.PMID:38484569 | DOI:10.1016/j.bbrc.2024.149764 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Ravi Shankar Kumar Tapasya Datta Hiteshwari Sinha Prabodh Kumar Trivedi Source Type: research

Mild hypothermia reduces lipopolysaccharide-induced microglial activation via down-regulation of Tent5c
In this study, we explore the differentially expressed (DE) mRNAs and long non-coding RNAs (lncRNAs) in BV-2 microglial cells under different conditions: normal temperature (CN), mild hypothermia (YT), normal temperature with lipopolysaccharide (LPS), and mild hypothermia with LPS (LPS + YT). Venn analysis revealed 119 DE mRNAs that were down-regulated in the LPS + YT vs LPS comparison but up-regulated in the CN vs LPS comparison, primarily enriched in Gene Ontology terms related to immune and inflammatory responses. Furthermore, through Venn analysis of YT vs CN and LPS + YT vs LPS comparisons, we identified 178 DE mRNAs ...
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Wen-Xian Ma Shao-Wen Wang Qian-Wen Fan Yue-Yue Wang Chao-Qun Chu Dong Liu Zhen Guo Ji-Hui Tang Jia-Gen Wen Source Type: research

Natural compound Byakangelicin suppresses breast tumor growth and motility by regulating SHP-1/JAK2/STAT3 signal pathway
In conclusion, our current study indicated that Byakangelicin, a natural compound, inhibits SHP-1/JAK2/STAT3 signaling and thus blocks tumor growth and motility.PMID:38484571 | DOI:10.1016/j.bbrc.2024.149758 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Xiuzhen Shi Yuexing Lai Wenjing Liu Xi Zhang Yanqin Cang Source Type: research

Glucose stockpile in the intestinal apical brush border in C. elegans
Biochem Biophys Res Commun. 2024 Mar 7;706:149762. doi: 10.1016/j.bbrc.2024.149762. Online ahead of print.ABSTRACTRevealing the mechanisms of glucose transport is crucial for studying pathological diseases caused by glucose toxicities. Numerous studies have revealed molecular functions involved in glucose transport in the nematode Caenorhabditis elegans, a commonly used model organism. However, the behavior of glucose in the intestinal lumen-to-cell remains elusive. To address that, we evaluated the diffusion coefficient of glucose in the intestinal apical brush border of C. elegans by using fluorescent glucose and fluores...
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Takumi Saito Kenji Kikuchi Takuji Ishikawa Source Type: research

The ligand binding domain of a type IV pilus chemoreceptor PilJ has a different fold from that of another PilJ-type receptor McpN
Biochem Biophys Res Commun. 2024 Mar 10;706:149765. doi: 10.1016/j.bbrc.2024.149765. Online ahead of print.ABSTRACTBacterial chemoreceptors sense the extracellular signals and regulate bacterial motilities, biofilm formation, etc. The periplasmic ligand binding domains of chemoreceptors occur as different structural folds and recognize a diversity of chemical molecules. In Pseudomonas aeruginosa (PAO1), two bacterial chemoreceptors, McpN (PA2788) and PilJ (PA0411), are proposed to both contain a PilJ-like ligand-binding domain (LBD) (Pfam motif PF13675) and involved in nitrate chemotaxis and type IV pilus-mediated motility...
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Rui Cui Qi-An Wang Lu Guo De-Feng Li Source Type: research

Identification of HK3 as a promising immunomodulatory and prognostic target in sepsis-induced acute lung injury
CONCLUSIONS: It was observed for the first time that HK3 plays a crucial role in the progression of sepsis-induced ALI and may be a valuable target for immunomodulation and therapy.Bioinformatics analysis identified HK3, MMP9, and S100A8 as hub genes with diagnostic and prognostic relevance in sepsis-induced ALI. Experimental findings showed increased HK3 expression in the lung tissue of septic mice, particularly in bronchial and alveolar epithelial cells. In vitro experiments demonstrated increased HK3 levels in lung epithelial cells stimulated with LPS, with cytoplasmic localization near the nucleus. Knockdown of HK3 exp...
Source: Biochemical and Biophysical Research communications - March 14, 2024 Category: Biochemistry Authors: Mingyu Zhu Xiaokai Tang Jingjing Xu Yuanqi Gong Source Type: research