Biochemical and Biophysical Research communications This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Cardiac-specific deletion of heat shock protein 60 induces mitochondrial stress and disrupts heart development in mice
Biochem Biophys Res Commun. 2024 Apr 4;710:149883. doi: 10.1016/j.bbrc.2024.149883. Online ahead of print.ABSTRACTCongenital heart diseases are the most common birth defects around the world. Emerging evidence suggests that mitochondrial homeostasis is required for normal heart development. In mitochondria, a series of molecular chaperones including heat shock protein 60 (HSP60) are engaged in assisting the import and folding of mitochondrial proteins. However, it remains largely obscure whether and how these mitochondrial chaperones regulate cardiac development. Here, we generated a cardiac-specific Hspd1 deletion mouse m...
Source: Biochemical and Biophysical Research communications - April 8, 2024 Category: Biochemistry Authors: Tao Shen Shuting Wang Can Huang Siting Zhu Xiangbin Zhu Na Li Hong Wang Lei Huang Mingming Ren Zhen Han Jianjun Ge Ze'e Chen Kunfu Ouyang Source Type: research

Disease-modifying potential of diphenyl diselenide in an experimental osteoarthritis model
Biochem Biophys Res Commun. 2024 Apr 4;710:149885. doi: 10.1016/j.bbrc.2024.149885. Online ahead of print.ABSTRACTOxidative stress is a key factor in the disruption of cartilage homeostasis during the development of osteoarthritis (OA). Organic selenium (Se)-containing compounds such as diselenides have excellent antioxidant activity and may prevent related diseases. We aimed to examine the benefits of the synthetic small molecule diphenyl diselenide (DPDSe) in OA models in vitro and in vivo. Our findings showed that DPDSe could maintain extracellular matrix (ECM) homeostasis and inhibit reactive oxygen species (ROS) produ...
Source: Biochemical and Biophysical Research communications - April 8, 2024 Category: Biochemistry Authors: Li Qiao Zhiyao Li Peihua Shi Source Type: research

Overcoming statin resistance in prostate cancer cells by targeting the 3-hydroxy-3-methylglutaryl-CoA-reductase
Biochem Biophys Res Commun. 2024 Mar 28;710:149841. doi: 10.1016/j.bbrc.2024.149841. Online ahead of print.ABSTRACTProstate cancer is the most prevalent malignancy in men. While diagnostic and therapeutic interventions have substantially improved in recent years, disease relapse, treatment resistance, and metastasis remain significant contributors to prostate cancer-related mortality. Therefore, novel therapeutic approaches are needed. Statins are inhibitors of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the rate-limiting enzyme of the mevalonate pathway which plays an essential role in cholesterol homeostasis. N...
Source: Biochemical and Biophysical Research communications - April 8, 2024 Category: Biochemistry Authors: Andy G öbel Sophie P ählig Anja Motz Dorit Breining Sofia Traikov Lorenz C Hofbauer Tilman D Rachner Source Type: research

S100A9 exacerbates sepsis-induced acute lung injury via the IL17-NF κB-caspase-3 signaling pathway
CONCLUSIONS: S100A9 as a key biomarker of sepsis-induced acute lung injury, and exacerbates lung damage and epithelial cell apoptosis induced by LPS via the IL-17-NFκB-caspase-3 signaling pathway.PMID:38588614 | DOI:10.1016/j.bbrc.2024.149832 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 8, 2024 Category: Biochemistry Authors: Hui Pei Jianming Chen Jie Qu Zhongqiu Lu Source Type: research

Photobiomodulation inhibits neuronal firing in the superficial but not deep layer of a rat spinal dorsal horn
In this study, we examined the effect of PBM of the sciatic nerve on firing in the deep layers of the spinal dorsal horn evoked by mechanical stimulation. Before and after PBM, mechanical stimulation was administered to the cutaneous receptive field using 0.6-26.0 g von Frey filaments (vFFs), and vFF-evoked firing in the deep layers of the spinal dorsal horn was recorded. The vFF-evoked firing frequencies were not altered after the PBM for any of the vFFs. The inhibition rate for 26.0 g vFF-evoked firing was approximately 13 % in the deep layers and 70 % in the superficial layers. This suggests that PBM selectively inhibit...
Source: Biochemical and Biophysical Research communications - April 7, 2024 Category: Biochemistry Authors: Daisuke Uta Naoya Ishibashi Shinichi Tao Masahito Sawahata Toshiaki Kume Source Type: research

Semaglutide alters gut microbiota and improves NAFLD in db/db mice
In this study, we used a gene mutation mouse model (db/db mice) to investigate the potential liver-improving effects of semaglutide. The results showed that semaglutide improved lipid levels and glucose metabolism in db/db mice. HE staining and oil red staining showed alleviation of liver damage and reduction of hepatic lipid deposition after injection of semaglutide. In addition, semaglutide also improved the integrity of gut barrier and altered gut microbiota, especially Alloprevotella, Alistpes, Ligilactobacillus and Lactobacillus. In summary, our findings validate that semaglutide induces modifications in the compositi...
Source: Biochemical and Biophysical Research communications - April 7, 2024 Category: Biochemistry Authors: Tuohua Mao Chenxuan Zhang Shuang Yang Yingying Bi Man Li Jia Yu Source Type: research

Measurement of the rotational relaxation time of intracellular water in dried yeast and Jurkat cells by near infrared spectroscopy
Biochem Biophys Res Commun. 2024 Mar 29;710:149857. doi: 10.1016/j.bbrc.2024.149857. Online ahead of print.ABSTRACTMolecular mobility of intracellular water is a crucial parameter in the study of the mechanism of desiccation tolerance. As one of the parameters that reflecting molecular mobility, the viscosity of intracellular water has been found intimately related with the protection of the phospholipid membrane because it quantifies the diffusion ability of water and mass in the intracellular environment. In this work we measured the intracellular water relaxation time, which can be translated into water viscosity, by us...
Source: Biochemical and Biophysical Research communications - April 7, 2024 Category: Biochemistry Authors: Junkai Zhang Ryo Shirakashi Source Type: research

Maackia amurensis seed lectin (MASL) and soluble human podoplanin (shPDPN) sequence analysis and effects on human oral squamous cell carcinoma (OSCC) cell migration and viability
Biochem Biophys Res Commun. 2024 Apr 3;710:149881. doi: 10.1016/j.bbrc.2024.149881. Online ahead of print.ABSTRACTMaackia amurensis lectins serve as research and botanical agents that bind to sialic residues on proteins. For example, M. amurensis seed lectin (MASL) targets the sialic acid modified podoplanin (PDPN) receptor to suppress arthritic chondrocyte inflammation, and inhibit tumor cell growth and motility. However, M. amurensis lectin nomenclature and composition are not clearly defined. Here, we sought to definitively characterize MASL and its effects on tumor cell behavior. We utilized SDS-PAGE and LC-MS/MS to fi...
Source: Biochemical and Biophysical Research communications - April 7, 2024 Category: Biochemistry Authors: Ariel C Yin Cayla J Holdcraft Eamonn J Brace Tyler J Hellmig Sayan Basu Saumil Parikh Katarzyna Jachimowska Evelyne Kalyoussef Dylan Roden Soly Baredes Eugenio M Capitle David I Suster Alan J Shienbaum Caifeng Zhao Haiyan Zheng Kevin Balcaen Simon Devos J Source Type: research

Torsion affects the calculation of DNA twisting number
This study elucidated the impact of torsion on the calculation of DNA twisting numbers, aiming to resolve controversies in the calculation of DNA topology and provided accurate computational methods and theoretical foundations for related research.PMID:38583234 | DOI:10.1016/j.bbrc.2024.149856 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 7, 2024 Category: Biochemistry Authors: Huimin Yang Xuguang Shi Source Type: research

ELISA-based highly sensitive assay system for the detection of endogenous NGLY1 activity
Biochem Biophys Res Commun. 2024 Mar 28;710:149826. doi: 10.1016/j.bbrc.2024.149826. Online ahead of print.ABSTRACTCytosolic peptide:N-glycanase (NGLY1, PNGase) is an enzyme that cleaves N-glycans from misfolded glycoproteins. In 2012, a human genetic disorder, NGLY1 deficiency, was first reported to be caused by mutations of the NGLY1 gene. Since then, there has been rapid progresses on NGLY1 biology, and gene therapy has been proposed as a promising therapeutic option for NGLY1 deficiency. While a plasma/urine biomarker has also been developed for this disease, detection of NGLY1 activity could be another viable option f...
Source: Biochemical and Biophysical Research communications - April 6, 2024 Category: Biochemistry Authors: Haruhiko Fujihira Keiko Sato Yuji Nishiuchi Takefumi Murase Yuka Matsuda Yukiko Yoshida Takayuki Kamei Tadashi Suzuki Source Type: research

Effects of LP533401 on vascular and bone calcification in hyperlipidemic mice
Biochem Biophys Res Commun. 2024 Mar 31;710:149854. doi: 10.1016/j.bbrc.2024.149854. Online ahead of print.ABSTRACTPeripheral serotonin levels are associated with cardiovascular disease risk. We previously found that serum serotonin levels are higher in hyperlipidemic mice than wild-type mice. Evidence also suggests that serotonin regulates biomineralization, in that serotonin treatment augments TNF-a-induced matrix calcification of aortic valve interstitial cells and that a selective inhibitor of peripheral serotonin, LP533401, rescues bone loss induced by ovariectomy in mice. Thus, in the present study, we examined the e...
Source: Biochemical and Biophysical Research communications - April 6, 2024 Category: Biochemistry Authors: Stuti Pradhan Andy Hon Yuxuan Xia Sophia Kalanski Nora Safvati Mimi Lu Linda L Demer Yin Tintut Source Type: research