Biochemical and Biophysical Research communications This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Plasmid expression of Deinococcus radiodurans RecA confers UV-A protection to Escherichia coli with an inverse protein dose dependence, which does not exceed conspecific RecA protection
Biochem Biophys Res Commun. 2024 Apr 4;710:149890. doi: 10.1016/j.bbrc.2024.149890. Online ahead of print.ABSTRACTLow level expression in Escherichia coli of the RecA protein from the radiation resistant bacterium Deinococcus radiodurans protects a RecA deficient strain of E. coli from UV-A irradiation by up to ∼160% over basal UV-A resistance. The protection effect is inverse protein dose dependent: increasing the expression level of the D. radiodurans RecA (DrRecA) protein decreases the protection factor. This inverse protein dose dependence effect helps resolve previously conflicting reports of whether DrRecA expressi...
Source: Biochemical and Biophysical Research communications - April 12, 2024 Category: Biochemistry Authors: Olga A Chinchilla Vince J LiCata Source Type: research

Strong evolutionary constraints against amino acid changes in the P2 subdomain of sapovirus GI.1 capsid protein VP1
Biochem Biophys Res Commun. 2024 Apr 2;710:149878. doi: 10.1016/j.bbrc.2024.149878. Online ahead of print.ABSTRACTSapovirus (SaV) is a nonenveloped RNA virus that causes acute gastroenteritis in humans. Although SaV is a clinically important pathogen in children, an effective vaccine is currently unavailable. The capsid protein VP1 of SaVs forms the outer shell of the virion and is highly diverse, as often seen in the virion-surface proteins of RNA viruses, creating an obstacle for vaccine development. We here report a unique phenomenon pertaining to the variation of SaV VP1. Phylogenetic and information entropy analyses u...
Source: Biochemical and Biophysical Research communications - April 12, 2024 Category: Biochemistry Authors: Masaru Yokoyama Yen Hai Doan Kazushi Motomura Hironori Sato Tomoichiro Oka Source Type: research

Protopine protects chondrocytes from undergoing ferroptosis by activating Nrf2 pathway
This study aimed to investigate whether PTP can effectively protect chondrocytes from ferroptosis. Primary mouse chondrocytes were treated with tert-butyl hydroperoxide (TBHP) to simulate oxidative stress in an in vitro model of osteoarthritis. Two concentrations of PTP (10 and 20 μg/mL) were validated for in vitro experiments. Cellular inflammation and metabolism were detected using RT-qPCR and western blotting (WB). Ferroptosis was assessed via WB, qPCR, reactive oxygen species (ROS) levels, lipid ROS, and immunofluorescence staining. In vitro, PTP significantly ameliorated chondrocyte inflammation and cytolytic metabol...
Source: Biochemical and Biophysical Research communications - April 12, 2024 Category: Biochemistry Authors: Hongjie Chen Yiming Zhong Weilin Sang Cong Wang Haiming Lu Peng Lai Libo Zhu Jinzhong Ma Source Type: research

Acesulfame potassium upregulates PD-L1 in HCC cells by attenuating autophagic degradation
Biochem Biophys Res Commun. 2024 Apr 9;711:149921. doi: 10.1016/j.bbrc.2024.149921. Online ahead of print.ABSTRACTArtificial sweeteners, which contain no or few calories, have been widely used in various foods and beverages, and are regarded as safe alternatives to sugar by the Food and Drug Administration. While several studies suggest that artificial sweeteners are not related to cancer development, some research has reported their potential association with the risk of cancers, including hepatocellular carcinoma (HCC). Here, we investigated whether acesulfame potassium (Ace K), a commonly used artificial sweetener, indu...
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Dong-Ho Kim Eun-Jun Kwon Keun-Gyu Park Jonghwa Jin Jun-Kyu Byun Source Type: research

Synergistic effects of graphene microgrooves and electrical stimulation on M2 macrophage polarization
In this study, we prepared a graphene free-standing substrate with 20 μm microgrooves using capillary forces induced by water evaporation. Subsequently, we established an ES cell culture platform for macrophage cultivation by integrating a self-designed multi-well chamber cell culture device. We observed that graphene microgrooves, in combination with ES, significantly reduce cell spreading area and circularity. Results from immunofluorescence, ELISA, and flow cytometry demonstrate that the synergistic effect of graphene microgrooves and ES effectively promotes macrophage M2 phenotypic polarization. Finally, RNA sequencin...
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Yan Yan Yilin Zhang Kexin Li Yudong Li Wei Qian Wenbo Zhang Yue Wang Wensheng Ma Linhao Li Source Type: research

Intermittent administration of PTH for the treatment of inflammatory bone loss does not enhance entheseal pathological new bone formation
CONCLUSION: Experimental animal models of AS treated with iPTH show improvement in trabecular bone density, but not entheseal pathological bone formation，indicating it may be a potential treatment for inflammatory bone loss does in AS.PMID:38603833 | DOI:10.1016/j.bbrc.2024.149888 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Shuai Zhang Wenjun Hao Dongying Chen Siwen Chen Zihao Li Fangling Zhong Haitao Wang Jianru Wang Zhaomin Zheng Zhongping Zhan Guo Dai Hui Liu Source Type: research

Identification and validation of anoikis-related genes to clarify the prognosis and immune mechanisms of patients with low-grade glioma
CONCLUSIONS: This study confirmed that TIMP1 promoted the malignant progression of LGG by inhibiting anoikis, providing insights into LGG pathogenesis and potential therapeutic targets.PMID:38603834 | DOI:10.1016/j.bbrc.2024.149894 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Ji Wang Yantin Liu Min Qian Wen Xu Jianfeng Chen Yuanguo Deng Ran Luo Jian Chen Wenxue Jia Longhu Liu Chunlei Tian Source Type: research

The effect of Schizophyllan on the differentiation of osteoclasts and osteoblasts
In this study, its effects on bone cells, particularly osteoclasts and osteoblasts, were examined. We demonstrated that SPG dose-dependently inhibited osteoclastogenesis and reduced gene expression associated with osteoclast differentiation. SPG also decreased bone resorption and F-actin ring formation. This inhibition could have been due to the downregulation of transcription factors c-Fos and nuclear factor of activated T cells 1 (NFATc1) via the MAPKs (JNK and p38), IκBα, and PGC1β/PPARγ pathways. In coculture, SPG lowered osteoclastogenic activity in calvaria-derived osteoblasts by reducing macrophage colony-stimul...
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Soo-Ho Kim Keun Ha Park Jun Lee Seoung Hoon Lee Jeong-Hwa Baek Source Type: research

Melanin-concentrating hormone receptor 1 is discarded by exosomes after internalization
In this study, we report the extracellular secretion of MCHR1 via exosomes. The recruitment of MCHR1 to exosomes occurs subsequent to its internalization, which is induced by stimulation with the ligand MCH. Although a highly glycosylated form of MCHR1, potentially representing a mature form, is selectively recruited to exosomes, the MCHR1 transferred into other cells does not exhibit functionality. The truncation of MCHR1 at the C-terminus not only impairs its response to MCH but also hinders its recruitment to exosomes. These findings imply that functional MCHR1 could be secreted extracellularly via exosomes, a process t...
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Ryohei Yamada Momoka Michimae Akie Hamamoto Hiroshi Takemori Source Type: research

Neutrophil extracellular traps as a unique target in the treatment of inflammatory pain
In conclusion, these discoveries propose that obstructing or intervening with NETs introduces a novel therapeutic avenue for addressing inflammatory pain.PMID:38604072 | DOI:10.1016/j.bbrc.2024.149896 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Wanxiang Qin Yuping Li Jian Cui Bao Yu Lehua Yu Congwen Yang Source Type: research

Chronic restraint stress-induced hyperalgesia is modulated by the periaqueductal gray neurons projecting to the rostral ventromedial medulla in mice
Biochem Biophys Res Commun. 2024 Apr 6;710:149875. doi: 10.1016/j.bbrc.2024.149875. Online ahead of print.ABSTRACTStress-induced hyperalgesia (SIH) is induced by repeated or chronic exposure to stressful or uncomfortable environments. However, the neural mechanisms involved in the modulatory effects of the periaqueductal gray (PAG) and its associated loops on SIH development hav e not been elucidated. In the present study, we used chronic restraint stress (CRS)-induced hyperalgesia as a SIH model and manipulated neuronal activity via a pharmacogenetic approach to investigate the neural mechanism underlying the effects of d...
Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Qian Chen Mingwei Zhao Jiaxue Dong Kun Yang Source Type: research