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Real-time monitoring of cortical brain activity in response to acute pain using wide-area Ca < sup > 2+ < /sup > imaging
Biochem Biophys Res Commun. 2024 Mar 18;708:149800. doi: 10.1016/j.bbrc.2024.149800. Online ahead of print.ABSTRACTPrevious human and rodent studies indicated that nociceptive stimuli activate many brain regions that is involved in the somatosensory and emotional sensation. Although these studies have identified several important brain regions involved in pain perception, it has been a challenge to observe neural activity directly and simultaneously in these multiple brain regions during pain perception. Using a transgenic mouse expressing G-CaMP7 in majority of astrocytes and a subpopulation of excitatory neurons, we reco...
Source: Biochemical and Biophysical Research communications - March 24, 2024 Category: Biochemistry Authors: Chihiro Inami Makito Haruta Yasumi Ohta Motoshi Tanaka MinHye So Kazuya Sobue Yasemin Akay Kazuhiko Kume Jun Ohta Metin Akay Masahiro Ohsawa Source Type: research

Circadian rhythms in CYP2A5 expression underlie the time-dependent effect of tegafur on breast cancer
Biochem Biophys Res Commun. 2024 Mar 20;708:149813. doi: 10.1016/j.bbrc.2024.149813. Online ahead of print.ABSTRACTThe chemotherapeutic agent tegafur, a prodrug that prolongs the half-life of fluorouracil (5-FU), exerts antitumor effects against various cancers. Since tegafur is metabolized to 5-FU by CYP2A6 in the liver, the expression of CYP2A6 determines the effect of tegafur. Here, we report that the expression rhythm of Cyp2a5, a homolog of human CYP2A6, in female mice causes dosing time-dependent differences in tegafur metabolism. In the livers of female mice, CYP2A5 expression showed a circadian rhythm, peaking duri...
Source: Biochemical and Biophysical Research communications - March 24, 2024 Category: Biochemistry Authors: Yuya Yoshida Taiki Fukuda Tomohito Tanihara Naoki Nishikawa Serina Iwasa Satoka Adachi Orion Zaitsu Yuma Terada Ryotaro Tsukamoto Hideki Shimoshikiryo Kohei Fukuoka Fumiaki Tsurusaki Kengo Hamamura Kosuke Oyama Akito Tsuruta Satoru Koyanagi Naoya Matsunag Source Type: research

Calcium-induced upregulation of energy metabolism heats neurons during neural activity
In this study, we achieved simultaneous imaging of Ca2+ and temperature using the genetically encoded indicators, B-GECO and B-gTEMP. We then compared the spatiotemporal distributions of Ca2+ responses and temperature. Following neural excitation induced by veratridine, an activator of the voltage-gated Na+ channel, we observed an approximately 2 °C increase in cytosolic temperature occurring 30 s after the Ca2+ response. The temperature elevation was observed in the non-nuclear region, while Ca2+ increased throughout the cell body. Moreover, this temperature increase was suppressed under Ca2+-free conditions and by inhib...
Source: Biochemical and Biophysical Research communications - March 24, 2024 Category: Biochemistry Authors: Jiayang Wu Yutaka Shindo Kohji Hotta Cong Quang Vu Kai Lu Tetsuichi Wazawa Takeharu Nagai Kotaro Oka Source Type: research