Biochemical and Biophysical Research communications This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Henlea earthworm bioluminescence comprises violet-blue BRET from tryptophan 2-carboxylate to deazaflavin cofactor
Biochem Biophys Res Commun. 2024 Mar 13;708:149787. doi: 10.1016/j.bbrc.2024.149787. Online ahead of print.ABSTRACTWe recently identified the deazaflavin cofactor as a light emitter in novel bioluminescence (BL) system from Siberian earthworms Henlea sp. (Petushkov et al., 2023, Org. Biomol. Chem. 21:415-427). In the present communication we compared in vitro BL spectra in the absence and in the presence of the cofactor and found a wavelength shift from 420 to 476 nm. This violet-blue BRET to deazaflavin cofactor (acceptor of photonless transfer) masks the actual oxyluciferin as an emitter (BRET donor) in the novel BL syst...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Valentin N Petushkov Matvey V Vavilov Anastasia N Khokhlova Renata I Zagitova Olga A Belozerova Aleksandr S Shcheglov Sergey I Kovalchuk Aleksandra S Tsarkova Natalia S Rodionova Ilia V Yampolsky Maxim A Dubinnyi Source Type: research

Distinct induction pathways of heat shock protein 27 in human keratinocytes: Heat stimulation or capsaicin through phosphorylation of heat shock factor 1 at serine 326 and/or suppression of ΔNp63
Biochem Biophys Res Commun. 2024 Mar 22;708:149817. doi: 10.1016/j.bbrc.2024.149817. Online ahead of print.ABSTRACTEpidermal keratinocytes, forming the outermost layer of the human body, serve as a crucial barrier against diverse external stressors such as ultraviolet radiation. Proper keratinocyte differentiation and effective responses to external stimuli are pivotal for maintaining barrier integrity. Heat is one such stimulus that triggers the synthesis of heat shock proteins (HSPs) when cells are exposed to temperatures above 42 °C. Additionally, activation of the transient receptor potential cation channel subfamily ...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Terufumi Kubo Kenta Sasaki Sayuri Sato Tomoyuki Minowa Tokimasa Hida Kenji Murata Takayuki Kanaseki Tomohide Tsukahara Yoshihiko Hirohashi Hisashi Uhara Toshihiko Torigoe Source Type: research

Regulation of inflammatory response by LINC00346 via miR-25-3p-mediated modulation of the PTEN/PI3K/AKT/NF- κB pathway
Biochem Biophys Res Commun. 2024 Mar 25;709:149828. doi: 10.1016/j.bbrc.2024.149828. Online ahead of print.ABSTRACTLong intergenic non-coding RNA 346 (LINC00346) has been reported to be involved in the development of atherosclerosis and specific cancers by affecting signaling pathways. However, its function in inflammation has not been thoroughly studied. Therefore, its expression pattern and function were determined in the human macrophage-like cell line THP-1. Lipopolysaccharide (LPS) treatment induced the expression of LINC00346. LPS-induced NF-κB activation and proinflammatory cytokine expression were suppressed or en...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Min-Ji Kim Su-Geun Lim Dong-Hyung Cho Jun-Yeong Lee Kyoungho Suk Won-Ha Lee Source Type: research

Polylevodopa nanoplatform for lateral flow immunochromatography assay of SARS-CoV-2 and influenza A virus
Biochem Biophys Res Commun. 2024 Mar 23;709:149821. doi: 10.1016/j.bbrc.2024.149821. Online ahead of print.ABSTRACTAt the end of 2019, an unprecedented outbreak of novel coronavirus pneumonia ravaged the global landscape, inflicting profound harm upon society. Following numerous cycles of transmission, we find ourselves in an epoch where the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coexists alongside influenza viruses (Flu A). Swift and accurate diagnosis of SARS-CoV-2 and Flu A is imperative to stem the spread of these maladies and administer appropriate treatment. Presently, colloidal gold-based later...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Kangsong He Yabing Ye Shang Liu Pengcheng Yuan Wenjing Sun Jianbin Tang Source Type: research

pH Dependence of HSF1 trimerization is shaped by intramolecular interactions
This study proposes a molecular mechanism based on the trimerization behavior of HSF1 at different pH values. Extensive mutagenesis of human and goldfish HSF1 revealed that the optimal pH for trimerization depended on the identity of residue 103. In particular, when residue 103 was occupied by tyrosine, a significant increase in the optimal pH was observed, regardless of the rest of the sequence. This behavior can be explained by the protonation state of the neighboring histidine residues, His101 and His110. Residue 103 plays a key role in trimerization by forming disulfide or non-covalent bonds with Cys36. If tyrosine res...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Bo-Hee Choi Chang-Ju Lee Tae Hwan Kim David Nahm-Joon Kim Young-Shang Park Jeong-Mo Choi Jang-Su Park Source Type: research

Agonists or positive allosteric modulators of α7 nicotinic acetylcholine receptor prevent interaction of SARS-Cov-2 receptor-binding domain with astrocytoma cells
Biochem Biophys Res Commun. 2024 Mar 25;709:149825. doi: 10.1016/j.bbrc.2024.149825. Online ahead of print.ABSTRACTSARS-Cov-2, the virus causing COVID-19, penetrates host target cells via the receptor of angiotensin-converting enzyme 2 (ACE2). Disrupting the virus interaction with ACE2 affords a plausible mechanism for prevention of cell penetration and inhibiting dissemination of the virus. Our studies demonstrate that ACE2 interaction with the receptor binding domain of SARS-Cov-2 spike protein (RBD) can be impaired by modulating the α7 nicotinic acetylcholine receptor (α7 nAChR) contiguous with ACE2. U373 cells of hum...
Source: Biochemical and Biophysical Research communications - March 27, 2024 Category: Biochemistry Authors: Olena Kalashnyk Olena Lykhmus Raymond Sullivan Serhiy Komisarenko Maryna Skok Source Type: research

Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway
In this study, we demonstrated that STING activation enhanced the expression of CD73 and the subsequent production of adenosine in immune cells and cancer cells. Mechanistically, the feedback activation of CD73 depended on the type I IFN/IFNAR axis induced by STING activation. Furthermore, the combination of STING agonist and anti-CD73 mAb markedly blocked tumor growth in vivo by promoting the infiltration of CD8+ T cells and reducing the accumulation of Foxp3+ regulatory T cells (Tregs) in the tumor microenvironment. Our work provides a rationale for the combination of STING agonists and CD73 inhibitors in cancer immunoth...
Source: Biochemical and Biophysical Research communications - March 26, 2024 Category: Biochemistry Authors: Nannan Fu Ziang Zhang Junmin Quan Source Type: research

Amiodarone inhibits the Toll-like receptor 3-mediated nuclear factor κB signaling pathway by blocking organelle acidification
Biochem Biophys Res Commun. 2024 Mar 19;708:149801. doi: 10.1016/j.bbrc.2024.149801. Online ahead of print.ABSTRACTToll-like receptor (TLR) agonists or pro-inflammatory cytokines converge to activate the nuclear factor κB (NF-κB) signaling pathway, which provokes inflammatory responses. In the present study, we identified amiodarone hydrochloride as a selective inhibitor of the TLR3-mediated NF-κB signaling pathway by screening the RIKEN NPDepo Chemical Library. In human umbilical vein endothelial cells (HUVEC), amiodarone selectively inhibited the expression of intercellular adhesion molecule-1 (ICAM-1) induced by poly...
Source: Biochemical and Biophysical Research communications - March 26, 2024 Category: Biochemistry Authors: Yuka Yokota Keiko Takaki Kosuke Baba Saki Sasaki Hiroyuki Hirano Hiroyuki Osada Takao Kataoka Source Type: research

Sesamin alleviates lipid accumulation induced by oleic acid via PINK1/Parkin-mediated mitophagy in HepG2 cells
Biochem Biophys Res Commun. 2024 Mar 22;708:149815. doi: 10.1016/j.bbrc.2024.149815. Online ahead of print.ABSTRACTSesamin, a special compound present in sesame and sesame oil, has been reported a role in regulating lipid metabolism, while the underlying mechanisms remain unclear. Autophagy has been reported associated with lipid metabolism and regarded as a key modulator in liver steatosis. The present work aimed to investigate whether sesamin could exert its protective effects against lipid accumulation via modulating autophagy in HepG2 cells stimulated with oleic acid (OA). Cell viability was evaluated using the CCK-8 m...
Source: Biochemical and Biophysical Research communications - March 26, 2024 Category: Biochemistry Authors: Mengyun Dong Tianliang Zhang Xueli Liang Xinyi Cheng Fuyan Shi Hang Yuan Fengxiang Zhang Qiqi Jiang Xia Wang Source Type: research