Correction to: Expanding Diversity and Common Goal of Regulatory T and B Cells. I: Origin, Phenotype, Mechanisms
The original article has been published without acknowledgment section. The acknowledgement section is given below for your reading. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - October 10, 2017 Category: Allergy & Immunology Source Type: research

The Effects of Intestinal Nematode L4 Stage on Mouse Experimental Autoimmune Encephalomyelitis
AbstractHelminths use various immunomodulatory and anti-inflammatory strategies to evade immune attack by the host. During pathological conditions, these strategies alter the course of disease by reducing immune-mediated pathology. The study examines the therapeutic effect of the nematode L4 stage based on an in vivo model of multiple sclerosis, monophasic encephalomyelitis (EAE), induced by sensitization with MOG35 –55 peptide in C57BL/6 female mice infected with the intestinal nematodeHeligmosomoides polygyrus. The EAE remission was correlated with altered leukocyte number identified in the central nervous system (...
Source: Archivum Immunologiae et Therapiae Experimentalis - October 3, 2017 Category: Allergy & Immunology Source Type: research

Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
This study focused on the genetic modification of human skeletal muscle cells (SkMCs). We chose myoblast cells due to their close biological resemblance to cardiomyocytes and the placental growth factor (PlGF) gene due to its pro-angiogenic potential. In our in vitro studies, we transfected SkMCs with thePlGF gene using electroporation, which has previously been proven to be efficient and generate robust overexpression of thePlGF gene and elevate PlGF protein secretion. Moreover, the functionality of the secreted pro-angiogenic proteins was confirmed using an in vitro capillary development assay. We have also examined the ...
Source: Archivum Immunologiae et Therapiae Experimentalis - September 26, 2017 Category: Allergy & Immunology Source Type: research

Novel Therapeutic Approaches to Atopic Dermatitis
AbstractAtopic dermatitis (AD) is one of the most common inflammatory skin diseases. The number of people affected by AD is relatively high and seems to be rising. Although mild and moderate forms of the disease can be well controlled by the use of emollients, topical corticosteroids, and topical calcineurin inhibitors, treatment of severe is still a huge challenge. The new hope is biologic drugs, magic bullets in allergy, targeted at different points of the complex pathomechanism of inflammation in AD. In this review, novel biologic therapies are discussed, including recombinant monoclonal antibodies directed against vari...
Source: Archivum Immunologiae et Therapiae Experimentalis - August 31, 2017 Category: Allergy & Immunology Source Type: research

Natural Agents-Mediated Targeting of Histone Deacetylases
AbstractIn the past few years, basic and clinical scientists have witnessed landmark achievements in many research projects, such as those conducted by the US National Institutes of Health Roadmap Epigenomics Mapping Consortium, the International Human Epigenome Consortium, The Cancer Genome Atlas Network and the International Cancer Genome Consortium, which have provided near-complete resolution of epigenetic landscape in different diseases. Furthermore, genome sequencing of tumors has provided compelling evidence related to frequent existence of mutations in readers, erasers and writers of epigenome in different cancers....
Source: Archivum Immunologiae et Therapiae Experimentalis - August 30, 2017 Category: Allergy & Immunology Source Type: research

Comparative Assessment of Induced Immune Responses Following Intramuscular Immunization with Fusion and Cocktail of LeIF, LACK and TSA Genes Against Cutaneous Leishmaniasis in BALB/c Mice
AbstractIn the present study, we evaluated induced immune responses following DNA vaccine containing cocktail or fusion of LeIF, LACK and TSA genes or each gene alone. Mice were injected with 100  µg of each plasmid containing the gene of insert, plasmid DNA alone as the first control group or phosphate buffer saline as the second control group. Then, cellular and humoral responses, lesion size were measured for all groups. All vaccinated mice induced Th1 immune responses againstLeishmania characterized by higher IFN- γ and IgG2a levels compared with control groups (p 
Source: Archivum Immunologiae et Therapiae Experimentalis - August 4, 2017 Category: Allergy & Immunology Source Type: research

The PD-1/PD-L1 Inhibitory Pathway is Altered in Primary Glomerulonephritides
AbstractThe pathogenesis of primary proliferative and non-proliferative glomerulonephritides (PGN and NPGN) is still not fully understood, however, current evidence suggests that most cases of PGN and NPGN are the results of immunologic response to different etiologic agents that activates various biological processes leading to glomerular inflammation and injury. Programmed cell death protein 1 (PD-1) is the major inhibitory receptor regulating T cell exhaustion. The aim of this study was to evaluate the frequencies of PD-1-positive and PD-ligand 1 (PD-L1)-positive T and B lymphocytes in patients with NPGN and PGN in rela...
Source: Archivum Immunologiae et Therapiae Experimentalis - August 2, 2017 Category: Allergy & Immunology Source Type: research

STING Signaling in Cancer Cells: Important or Not?
AbstractStimulator of interferon genes (STING) is an adaptor protein that plays an important role in the activation of type I interferons in response to cytosolic nucleic acid ligands. Recent evidence indicates involvement of the STING pathway in the induction of antitumor immune response. Therefore, STING agonists are now being extensively developed as a new class of cancer therapeutics. However, little is known about the consequences of activated STING-mediated signaling in cancer cells on the efficacy of the antitumor treatment. It has been shown that activation of the STING-dependent pathway in cancer cells can result ...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 26, 2017 Category: Allergy & Immunology Source Type: research

Role of TGF- β in Self-Peptide Regulation of Autoimmunity
AbstractTransforming growth factor (TGF)- β has been implicated in regulation of the immune system, including autoimmunity. We have found that TGF-β is readily produced by T cells following immunization with self-peptide epitopes that downregulate autoimmune responses in type 1 diabetes (T1D) prone nonobese diabetic (NOD) mice. These incl ude multiple peptide epitopes derived from the islet β-cell antigens GAD65 (GAD65 p202-221, GAD65 p217-236), GAD67 (GAD67 p210-229, GAD67 p225-244), IGRP (IGRP p123-145, IGRP p195-214) and insulin B-chain (Ins. B:9-23) that protected NOD mice from T1D. Immunization of NOD m...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 21, 2017 Category: Allergy & Immunology Source Type: research

Possible Immunomodulating Effect of Retinol on Cytokines Secretion in Patients with Recurrent Furunculosis
AbstractRecurrent furunculosis is an infection of hair follicles which results in formation of abscesses. Previous studies showed that the pathogenesis of the disease may include an immune-mediated component as the proliferative response of peripheral blood lymphocytes to staphylococcal antigen is depressed. The aim of our study was to evaluate cytokines concentration in the plasma of patients with recurrent furunculosis and to determine whether retinol affects the secretion of those cytokines in patients with recurrent furunculosis and healthy subjects. Blood samples were taken from 15 patients with recurrent furunculosis...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 20, 2017 Category: Allergy & Immunology Source Type: research

Tumor-Associated Macrophages as Target for Antitumor Therapy
AbstractIt is well known that the microenvironment of solid tumors is rich in inflammatory cells that influence tumor growth and development. Macrophages, called tumor-associated macrophages (TAMs), are the most abundant immune cell population present in tumor tissue. Several studies have demonstrated that the density of TAMs is associated with a poor prognosis and positively correlates with tumor growth. Several studies have proved that TAMs may activate and protect tumor stem cells, stimulate their proliferation as well as promote angiogenesis and metastasis. Furthermore, TAMs-derived cytokines and other proteins, such a...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 28, 2017 Category: Allergy & Immunology Source Type: research

Anti-GITR Antibody Treatment Increases TCR Repertoire Diversity of Regulatory but not Effector T Cells Engaged in the Immune Response Against B16 Melanoma
AbstractCrosslinking of glucocorticoid-induced TNF family-related receptor (GITR) with agonist antibodies restores cancer immunity by enhancing effector T cell (Teff) responses while interfering with intra-tumor regulatory T cell (Treg) stability and/or accumulation. However, how anti-GITR antibody infusion changes T cell receptor (TCR) repertoire of Teffs and Tregs engaged in anti-tumor immune response is unclear. Here, we used a transgenic mouse model (TCRmini) where T cells express naturally generated but limited TCR repertoire to trace the fate of individual T cells recognizing B16 melanoma in tumor-bearing mice, treat...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 21, 2017 Category: Allergy & Immunology Source Type: research

Skin Immunity
AbstractSkin is the largest organ of the body with a complex network of multitude of cell types that perform plastic and dynamic cellular communication to maintain several vital processes such as inflammation, immune response including induction of tolerance and disease prevention, wound healing, and angiogenesis. Of paramount importance are immunological functions of the skin that protect from harmful exposure coming from external and internal environments. Awareness of skin immunity can provide a better comprehension of inflammation, autoimmunity, cancer, graft-versus-host disease, vaccination, and immunotherapy approach...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 16, 2017 Category: Allergy & Immunology Source Type: research

Exocrine Gland Morphogenesis: Insights into the Role of Amphiregulin from Development to Disease
AbstractAmphiregulin (AREG) is a well-characterized member of the epidermal growth factor (EGF) family and is one of the ligands of the EGF receptor (EGFR). AREG plays a key role in mammalian development and in the control of branching morphogenesis in various organs. Furthermore, AREG participates in a wide range of physiological and pathological processes activating the major intracellular signalling cascades governing cell survival, proliferation and motility. In this article, we review current advances in exocrine glands morphogenesis, focusing on the salivary gland, and discuss the essential aspects of AREG structure,...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 7, 2017 Category: Allergy & Immunology Source Type: research

The Immune Response in Periodontal Tissues
AbstractThe uniqueness of periodontal diseases is caused by several factors. This group of diseases is caused by numerous bacterial species formed in the dental biofilm, and one cannot distinguish the specific pathogen that is responsible for the disease initiation or progress (though Gram-negative anaerobic rods are associated with the advanced form of the disease). The disease is both infectious and inflammatory in its nature, and in the state of health there is always a subclinical level of inflammatory response, caused by the so-called harmless bacteria. Negligence in oral hygiene may result in maturation of the biofil...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 6, 2017 Category: Allergy & Immunology Source Type: research

Heterogeneity Among Neutrophils
AbstractNeutrophils (PMNs) play a key role in innate defence mechanisms. Generally, PMNs were considered to have a homogeneous population of mature and diversified cells. It seems, however, that their pleiotropic action results from the existence of different subpopulations in this group of cells. There are data that confirm the involvement of PMNs in the direct activation of other cells in non-specific response, as well as specialised cells in specific response. For example, there have been observations of PMNs with different levels of activity in relation to lymphocytes, and a population was identified which had characte...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 30, 2017 Category: Allergy & Immunology Source Type: research

Interleukin (IL)-23 Receptor, IL-17A and IL-17F Gene Polymorphisms in Brazilian Patients with Rheumatoid Arthritis
AbstractRheumatoid arthritis (RA) is a progressive, autoimmune disease for which the previous studies have shown that some functional polymorphisms can influence its etiology. Knowing this, the aim of this study was to investigate the association of +2199 A/CIL-23R (rs10889677), −197 G/AIL-17A (rs2275913), and +7488 A/GIL-17F (rs763780) gene polymorphisms with RA susceptibility and clinical features in a Brazilian population. A total of 127 RA patients and 134 healthy controls were recruited for the analyses of polymorphic variants. Genotyping was performed using RFLP-PCR. Logistic regression was used to analyze the ...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 25, 2017 Category: Allergy & Immunology Source Type: research

Ficolin-2 Gene rs7851696 Polymorphism is Associated with Delayed Graft Function and Acute Rejection in Kidney Allograft Recipients
This study comprised 270 Caucasian deceased-donor renal transplant recipients. The following parameters were recorded in each case: delayed graft function (DGF), acute rejection (AR), and chronic allograft dysfunction. Among patients with DGF, we observed a significantly increased frequency of rs7851696 GT and TT genotypes as well as T allele (TT  + GT vs GG OR 1.98, 95% CI 1.12–3.48,p = 0.02; T vs G OR 2.08, 95% CI 1.27–3.41,p = 0.005). There was also an increased frequency of rs4521835 GG and TG genotypes as well as G alleles; however, these differences were on the borderline of ...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 23, 2017 Category: Allergy & Immunology Source Type: research

KIR , LILRB and their Ligands ’ Genes as Potential Biomarkers in Recurrent Implantation Failure
AbstractReproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine recept...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 18, 2017 Category: Allergy & Immunology Source Type: research

The Potential Role of Kr üppel-Like Zinc-Finger Protein Glis3 in Genetic Diseases and Cancers
AbstractGli-similar 3 (Glis3) belongs to a Glis subfamily of Kr üppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, β-cell maturation and maintenance, and insulin production. Examination of the DNA-binding domain of Glis3 reveals that this domain contains a repeated cysteine 2/histidine 2 (Cys2/His2) zinc-finger motif in the central region where the recognized DNA sequence binds. The loss of the production of pancreatic hormones, such as insulin 1 and 2, is linked to the down-regulation of β cel...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 18, 2017 Category: Allergy & Immunology Source Type: research

Expanding Diversity and Common Goal of Regulatory T and B Cells. I: Origin, Phenotype, Mechanisms
AbstractImmunosuppressive activity of regulatory T and B cells is critical to limit autoimmunity, excessive inflammation, and pathological immune response to conventional antigens or allergens. Both types of regulatory cells are intensively investigated, however, their development and mechanisms of action are still not completely understood. Both T and B regulatory cells represent highly differentiated populations in terms of phenotypes and origin, however, they use similar mechanisms of action. The most investigated CD4+CD25+ regulatory T cells are characterized by the expression of Foxp3+ transcription factor, which is n...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 5, 2017 Category: Allergy & Immunology Source Type: research

Expanding Diversity and Common Goal of Regulatory T and B Cells. II: In Allergy, Malignancy, and Transplantation
AbstractRegulation of immune response was found to play an important role in the course of many diseases such as autoimmune diseases, allergy, malignancy, organ transplantation. The studies on immune regulation focus on the role of regulatory cells (Tregs, Bregs, regulatory myeloid cells) in these disorders. The number and function of Tregs may serve as a marker of disease activity. As in allergy, the depletion of Tregs is observed and the results of allergen-specific immunotherapy could be measured by an increase in the population of IL-10+ regulatory cells. On the basis of the knowledge of anti-cancer immune response reg...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 3, 2017 Category: Allergy & Immunology Source Type: research

CD1: A Singed Cat of the Three Antigen Presentation Systems
AbstractContrary to general view that the MHC Class I and II are the kapellmeisters of recognition and response to antigens, there is another big player in that part of immunity, represented by CD1 glycoproteins. In contrast to MHC Class I or II, which present peptides, CD1 molecules present lipids. Humans express five CD1 proteins (CD1a-e), four of which (CD1a-d) are trafficked to the cell surface, where they may display lipid antigens to T-cell receptors. This interaction may lead to both non-cognate and cognate T cell help to B cells, the latter eliciting anti-lipid antibody response. All CD1 proteins can bind a broad r...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 6, 2017 Category: Allergy & Immunology Source Type: research

Human Gyrovirus-Apoptin Interferes with the Cell Cycle and Induces G2/M Arrest Prior to Apoptosis
In this study, we have investigated the effect of HGyv-Apoptin on cell cycle progression of cancer cells. We also compared HGyv-Apoptin ’s action to its homologue chicken anemia virus Apoptin (CAV-Apoptin). We show that HGyv-Apoptin induces G2/M arrest in cancer cells. This is at least in part due to the fact that HGyv-Apoptin induces an abnormal spindle formation in mitotic cells that do not progress properly throughout the cell cycle. HGyv-Apoptin most likely inhibits APC function leading to a sustained cyclin-B1-expression. These results indicate that HGyv-Apoptin has a similar mechanism of action as its homolog C...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 6, 2017 Category: Allergy & Immunology Source Type: research

Evaluation of Endothelial Function by Flow-Mediated Dilation: a Comprehensive Review in Rheumatic Disease
AbstractFlow-mediated dilation (FMD) represents a non-invasive marker of endothelial function to evaluate vascular homeostasis, which reflects the effects of several mechanisms, including vessel tone regulation, cell proliferation, and inflammatory responses. Beyond classical atherosclerotic risk factors such as arterial hypertension, diabetes mellitus, smoking, obesity, and dyslipidemia, chronic inflammation contributes to the endothelial dysfunction causing plaque formation and there is growing evidence of a significantly higher cardiovascular morbidity associated with autoimmune diseases. The endothelium reacts to sever...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 30, 2017 Category: Allergy & Immunology Source Type: research

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy
AbstractIn recent years, in the effort to find a potential innovative therapy for multiple sclerosis (MS), researchers focused on transplantation of mesenchymal stem cells (MSCs) due to their well-recognized ability to suppress inflammatory/autoimmune responses and exert neuroregenerative properties. MSCs are a heterogeneous subset of pluripotent non-hematopoietic stromal cells that can be isolated from many different adult tissues, characterized by the capability to differentiate into various cell lineages, and to translocate into damaged areas, providing immunomodulatory effects. To date, several encouraging results were...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 25, 2017 Category: Allergy & Immunology Source Type: research

Tumor-Associated Macrophages and Regulatory T Cells Infiltration and the Clinical Outcome in Colorectal Cancer
AbstractThe aim of the study is the assessment of the intensity of the infiltration of tumor-associated macrophages (TAMs) CD68+/iNOS− and Tregs CD8+/FoxP3+ in colorectal cancer (CRC) patients as prognostic factors with respect to disease-free survival (DFS) and overall survival (OS). In this retrospective study, tissue samples were obtained from 89 patients undergoing resection for CRC (stage IIA, pT3N0M0 and stages IIIB and IIIC, pT3N1-2M0). Recurrence was observed in 45 patients at the time of the follow-up (10 local recurrences, 35 distant metastases). In patients with recurrence the following were present: a ten...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 25, 2017 Category: Allergy & Immunology Source Type: research

Acute Thymic Involution and Mechanisms for Recovery
AbstractAcute thymic involution (ATI) is usually regarded as a virulence trait. It is caused by several infectious agents (bacteria, viruses, parasites, fungi) and other factors, including stress, pregnancy, malnutrition and chemotherapy. However, the complex mechanisms that operate during ATI differ substantially from each other depending on the causative agent. For instance, a transient reduction in the size and weight of the thymus and depletion of populations of T cell subsets are hallmarks of ATI in many cases, whereas severe disruption of the anatomical structure of the organ is also associated with some factors, inc...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 22, 2017 Category: Allergy & Immunology Source Type: research

Perillyl alcohol, a pleiotropic natural compound suitable for brain tumor therapy, targets free radicals
AbstractMonoterpenes such as limonene and perillyl alcohol (POH) are promising natural compounds with pro-oxidant properties partly due to endoplasmic reticulum (ER) stress-induced cytotoxicity, and antioxidant activity owing to their activity as free radical scavengers, inhibition of coenzyme Q synthesis, activation of antioxidant-responsive elements (inducing detoxification enzymes) and induction of apoptosis. Activation of ER-stress responses generates reactive oxygen species (ROS), which are highly reactive free radicals mainly produced during mitochondrial electron transfer for adenosine triphosphate (ATP) synthesis. ...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 16, 2017 Category: Allergy & Immunology Source Type: research

Effects of IFN- β1a and IFN-β1b treatment on the expression of cytokines, inducible NOS (NOS type II), and myelin proteins in animal model of multiple sclerosis
AbstractThe aim of this study was to investigate the effects of interferon (IFN)- β1a and IFN-β1b treatment on inflammatory factors and myelin protein levels in the brain cortex of the Lewis rat experimental autoimmune encephalomyelitis (EAE), animal model of multiple sclerosis. To induce EAE, rat were immunized with inoculums containing spinal cord guinea pig homogenized in ph osphate-buffered saline and emulsified in Freund’s complete adjuvant containing 110 µg of the appropriate antigen in 100 µl of an emulsion and additionally 4-mg/mlMycobacterium tuberculosis (H37Ra). The rats wer...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 14, 2017 Category: Allergy & Immunology Source Type: research

Pre-Pregnancy Levels of Peripheral Natural Killer Cells as Markers for Immunomodulatory Treatment in Patients with Recurrent Miscarriage
AbstractImmunological risk factors in patients with recurrent miscarriage (RM) are discussed controversially. Abnormalities of natural killer cells (NK) have been described in RM patients. Lipid infusions are known to modulate lymphocyte subsets. The aim of this study was to identify immune parameters that predict success of treatment with lipid infusions in RM patients with elevated NK. In sum,n = 341 couples with RM were screened for established risk factors and peripheral lymphocyte subpopulations as well as uterine NK cells. We identifiedn = 136 patients with ≥ 2 consecutive RM and...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 10, 2017 Category: Allergy & Immunology Source Type: research

Neuronal Differentiation Capability of Nasal Polyps of Chronic Rhinosinusitis
AbstractChronic rhinosinusitis with nasal polyps is considered a subgroup of chronic rhinosinusitis and a significant health problem, but the pathogenesis remains unclear to date. Therefore, we investigated the stemness to determine the role of stem cells in nasal polyps, with additional analysis of the neuronal differentiation potential of nasal polyp cells. We determined gene and protein expression profiles of stem cells in nasal polyp tissues, using whole genome microarray, quantitative real-time PCR (qPCR), immunohistochemistry, and flow cytometry. To evaluate the neuronal differentiation potential of nasal polyp cells...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 8, 2017 Category: Allergy & Immunology Source Type: research

Sildenafil, a Phosphodiesterase Type 5 Inhibitor, Downregulates Osteopontin in Human Peripheral Blood Mononuclear Cells
AbstractThe aim of this study was to investigate the ability of sildenafil to regulate osteopontin (OPN) gene and protein in peripheral blood mononuclear cells (PBMCs) from healthy blood donors. OPN is expressed by a wide variety of cell types, including immune cells. OPN functions are linked to various physiological and pathological conditions. Sildenafil is a selective inhibitor of type 5 phosphodiesterase. Sildenafil has recently been found to have immunomodulatory effects in animal models and in studies performed in humans. PMA-stimulated and unstimulated PBMCs from 16 healthy blood donors (men) were cultured with sild...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 15, 2017 Category: Allergy & Immunology Source Type: research

The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients
AbstractThe rich bacterial flora of oral cavity is controlled by innate immune response, including antibacterial peptides and among them human neutrophil peptides 1 –3 (HNP1-3). The knowledge of the involvement of HNPs in innate and acquired immunity of the periodontium is fragmentary. The aim of the study was to assess alterations in HNP1-3 levels in the gingival crevicular fluid (GCF) of chronic periodontitis patients before and after nonsurgical periodonta l therapy. Nineteen patients with chronic periodontitis were qualified to the study. After periodontal examination, one site with pocket depth (PD) ≥4...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 14, 2017 Category: Allergy & Immunology Source Type: research

Identification of Clinicopathological Spectrum, Platelet Glycoprotein IIb/IIIa complex and Platelet Antibodies in Egyptian Children with Glanzmann ’s Thrombasthenia
In this study, we have delineated that type I is the most common type of GT in Upper Egypt. Our data suggested that there is a good correlation between quantitative changes in the surface expression of platelet membrane glycoproteins detected by flow cytometry and the clinical severity of bleeding. Therefore, classifying of severity of bleeding in patients with GT could possibly aid the pediatricians and hematologists in the implementation of ideal prophylactic measures. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - February 3, 2017 Category: Allergy & Immunology Source Type: research

Primary Biliary Cholangitis Associated with Skin Disorders: A Case Report and Review of the Literature
We report an unusual case of PBC associated with acquired reactive perforating dermatosis (ARPD) and present a review of the literature on skin disorders associated with PBC. Our patient presented to the dermatology department with generalized pruritus associated with nodular perforating skin lesions on the trunk, and cholestatic liver disease of unknown origin. After having established both diagnosis of ARPD and PBC, she was managed in an interdisciplinary manner, and both her skin and liver conditions improved gradually. Only one similar case is reported in the literature, in that case, the liver disease was not treated....
Source: Archivum Immunologiae et Therapiae Experimentalis - January 24, 2017 Category: Allergy & Immunology Source Type: research

Proceedings of the 10th Jubilee East –West Immunogenetics Conference on April 21–23, 2016 (Wrocław, Poland)
(Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - January 20, 2017 Category: Allergy & Immunology Source Type: research

Functions of Cancer-Derived Extracellular Vesicles in Immunosuppression
AbstractExtracellular vesicles, including exosomes, constitute an important element of intercellular communication by carrying a variety of molecules from producer to target cells. The transport of mRNA and miRNA can directly modulate gene expression in the target cells. The miRNA content in exosomes is characteristic for the cell from which the vesicles were derived enabling the usage of exosomes as biomarkers for the diagnosis various diseases, including cancer. Cancer-derived exosomes support the survival and progression of tumors in many ways and also contribute to the neutralization of the anti-cancer immune response....
Source: Archivum Immunologiae et Therapiae Experimentalis - January 17, 2017 Category: Allergy & Immunology Source Type: research

An Association Between Functional Polymorphisms of the Interleukin 1 Gene Complex and Schizophrenia Using Transmission Disequilibrium Test
AbstractIL1 gene complex has been implicated in the etiology of schizophrenia. To assess whether IL1 gene complex is associated with susceptibility to schizophrenia in Polish population we conducted family-based study. Functional polymorphisms from IL1A (rs1800587, rs17561, rs11677416), IL1B (rs1143634, rs1143643, rs16944, rs4848306, rs1143623, rs1143633, rs1143627) and IL1RN (rs419598, rs315952, rs9005, rs4251961) genes were genotyped in 143 trio with schizophrenia. Statistical analysis was performed using transmission disequilibrium test. We have found a trend toward an association of rs1143627, rs16944, rs1143623 in IL1...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Profile of Inflammation-Associated Proteins in Early Post-Transplant Samples of Patients After Allogeneic Hematopoietic Stem Cell Transplantation: a Preliminary Study
AbstractAllogeneic hematopoietic stem cell transplantation (aHSCT) is used as a curative treatment in severe hematological and immunological disorders. Despite clear improvement of the aHSCT outcome, substantial proportion of patients still suffers from severe complications, including graft-versus-host disease (GvHD). The aim of this study was, therefore, to identify inflammation-associated molecules deregulated in the early serum samples of the patients after aHSCT and nominate markers associated with particular aHSCT parameters/complications. Serum concentrations of 92 inflammation-associated proteins were measured in sa...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Transplantology: Challenges for Today
AbstractClinical transplantology in Poland had its 50th anniversary this year. With the early and long results comparable to the best achieved in the world leading centers, we face old and completely new challenges for this medical speciality. Main and growing challenge is insufficient number of available organs. With less than 15  donors/mln population/year Poland stay in the lower row of European countries in this measurement of transplant activity. Donation system is not efficient enough and we lose a big number of potential donors still. Living donation (with the exception for the fragments of the liver) remains l...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Characterization of Three CYP2C19 Gene Variants by MassARRAY and Point of Care Techniques: Experience from a Czech Centre
AbstractDistribution of cytochrome P450 2C19 enzyme gene (CYP2C19) variants affecting metabolism of clopidogrel was determined in 526 Czech patients after percutaneous coronary intervention using MassARRAY genotyping and compared to distribution in other populations of European descent. Fifty-three (10%) patients underwent parallel determination ofCYP2C19 genotypes from buccal swabs by a point of care technique with 100% concordance to the main genotyping platform. ObservedCYP2C19 genotypes were related to clopidogrel metabolism phenotypes and discussed in population context. Hereby, presented methodologies provide accurat...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Apportioning Blame: Autoreactive CD4 + and CD8 + T Cells in Type 1 Diabetes
AbstractType 1 diabetes (T1D) is one of the most studied archetypal organ-specific autoimmune diseases. Although many clinical, epidemiological, and pathological characteristics have been described, there are still important issues which need to be resolved as these will have a major impact on the development of future antigen-specific immunotherapies. An important question relates to T lymphocytes in the development of the disease, in particular their role in the destruction of insulin-producing beta cells. Since the discovery that certain class II histocompatibility complex molecules (HLA) are linked to the development o...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Distribution of HLA-DQB1 in Czech Patients with Central Hypersomnias
AbstractThe aim of our study was to analyze the distribution of HLA-DQB1 in Czech patients with central hypersomnias and differences between diagnostic groups of narcolepsy type 1 (NT1), type 2 (NT2), idiopathic hypersomnia (IH) and no central hypersomnia subjects (no-CH). Statistical analysis of DQB1 genotyping was performed on the cohort of 716 patients (375 men, 341 women) with reported excessive daytime sleepiness. DQB1*06:02 allele was present in 94% of the NT1 patients. The decrease of DQB1*06:03 allele was also confirmed. No other DQB1*06 allele nor any other DQB1 allele group was differently distributed in the NT1....
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Mutation c.256_257delAA in RAG1 Gene in Polish Children with Severe Combined Immunodeficiency: Diversity of Clinical Manifestations
In this study, we compare clinical symptoms and laboratory findings in four children with identical mutation inRAG1 gene. All of analyzed patients presented symptoms of severe combined immunodeficiencies associated or not with Omenn syndrome (OS) features. In our patients two different types of variants inRAG1 gene were detected. The first of the mutation was the deletion of AA dinucleotide at position c.256_257 (p.Lys86ValfsTer33), the second gene variant was substitution c.2867T>C (p.Ile956Thr). In Patient 1 we detected that compound heterozygous mutations involved both of the mentioned variants. Whereas, in Patients ...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Soluble BAFF Cytokine Levels and Antibody-Mediated Rejection of the Kidney Allograft
AbstractThe B-cell activating factor (BAFF) cytokine has important functions for the survival and maturation of B lymphocytes, which implies that this cytokine might play a role in the development of antibody-mediated rejection (AMR) after kidney transplantation. In our study, we compared the concentrations of the soluble BAFF cytokine in kidney graft recipients with AMR and patients without rejection with the goal of testing the hypothesis whether BAFF level measurement might be useful as a diagnostic marker of AMR. The study included a cohort of 19 high-risk patients with diagnosed AMR and 17 control patients free of rej...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

The Influence of HLA and KIR Genes on Malignant Melanoma Development and Progression
AbstractMany studies have described the role of killer immunoglobulin-like receptors (KIRs) and their cognate human leukocyte antigen (HLA) class I ligands in the immune protection against melanoma, but the effect of these markers on intra-individual variations in tumor development and progression has remained less clear. We performed KIR, HLA, and KIR/ligand analysis in 283 patients with malignant melanoma in order to evaluate their integrated influence on disease stage and progression. The patients were grouped according to AJCC staging, histological type of the primary tumor, progression, and survival rate. Analysis of ...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Functional Polymorphism in the Interleukin 6 ( IL6 ) Gene with Respect to Depression Induced in the Course of Interferon- α and Ribavirin Treatment in Chronic Hepatitis Patients
AbstractInterleukin (IL)-6 is a multifactorial cytokine known to be increased in patients with chronic hepatitis C (CHC) and to be predictive of depression incidence. The aim of the study was to explore the association betweenIL6 gene C-174G polymorphism and depressive symptom severity in the longitudinal study design following the course of pegylated interferon/ribavirin treatment in CHC patients. In our study, we included 62 CHC subjects. They were assessed using present state examination, Beck Depression Inventory (BDI) and Montgomery Åsberg Depression Rating Scale (MADRS) at weeks 0, 3, 5, 9, 13, 24 and 24 w...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Genetic Structure of the Armenian Population
AbstractLocated at the crossroads of Europe and the Middle East, the Armenian Highland served as a transition corridor for major waves of prehistoric and historic migrations. The genetic history of Armenians as an indigenous population of the region attracts keen scientific interest to resolve the puzzle of ancient Middle Eastern populations ’ expansion and the spread of Indo-European languages. Here, we review the current state of studies on the genetic structure of both modern and ancient inhabitants of the Armenian Highland and outline further steps to be fulfilled in this regard. (Source: Archivum Immunologiae et...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research

Significance of Polymorphism and Expression of miR-146a and NFkB1 Genetic Variants in Patients with Rheumatoid Arthritis
AbstractMicroRNA-146a (miR-146a) has been shown to play an important role in the regulation of inflammatory innate immune responses, and found to be differentially expressed in rheumatoid arthritis (RA). Through NF- κB pathway, this molecule is able to stimulate the release of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-17. It has been also suggested that single-nucleotide polymorphisms (SNPs) in miRNA sequences may alter miRNA expression and that miR-146a rs2910164 SNP may contribute to RA devel opment. These observations prompted us to analyze the potential associations between themiR-146a-3p...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 11, 2017 Category: Allergy & Immunology Source Type: research