WNT7A Expression is Downregulated in T Lymphocytes after T-Cell Receptor Activation Due to Histone Modifications and in T-ALL by DNA Methylation
AbstractWNT signaling pathway regulates several processes involved in the homeostasis of normal cells. Its dysregulation is associated with pathological outcomes like cancer. We previously demonstrated that downregulation of WNT7A correlates with higher proliferation rates in acute lymphoblastic leukemia. However, the regulation of this gene in pathological and normal conditions remains unexplored. In this work, we aimed to analyze the transcriptional regulation of WNT7A in leukemic cells and in normal T lymphocytes after a proliferative stimulus. WNT7A expression was measured in blood cells and in T lymphocytes after phyt...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 26, 2020 Category: Allergy & Immunology Source Type: research

Exopolysaccharide from Lactobacillus rhamnosus KL37 Inhibits T Cell-dependent Immune Response in Mice
In this study, we used highly purified EPS fromL. rhamnosus KL37 (EPS-37) to verify its anti-inflammatory properties and the ability to suppress T cell-dependent humoral response. We have employed the model of active CIA, in which mice immunized with type II collagen (CII) along with LPS were treated with pure EPS-37. Intravenous administration of purified EPS-37 markedly ameliorated arthritis and reduced CII-specific antibody production. EPS-37 injected subcutaneously reduced the clinical symptoms of CIA but without the reduction of arthritogenic antibodies. In addition, the effect of EPS-37 on T-cell functions was tested...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 25, 2020 Category: Allergy & Immunology Source Type: research

Decreased Serum Adiponectin Reflects Low Vitamin D, High Interleukin 6, and Poor Physical Performance in Knee Osteoarthritis
This study aimed to investigate relationships of serum adiponectin, 25-hydroxyvitamin D (25(OH)D), interleukin (IL)-6, and physical performance in knee OA patients. A total of 175 knee OA patients and 52 healthy controls were recruited. Serum adiponectin, 25(OH)D, IL-6, biochemical markers, knee pain and functional scores, muscle strength, physical performance, metabolic parameters, and body composition were evaluated. Serum adiponectin levels were significantly higher in knee OA patients than that  in controls, while its serum levels were significantly decreased in obese patients, especially those with sarcopenia. Fu...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 24, 2020 Category: Allergy & Immunology Source Type: research

BW5147 and Derivatives for the Study of T Cells and their Antigen Receptors
AbstractLike B cells, T cells can be immortalized through hybridization with lymphoma cells, a technique that has been particularly useful in the study of the T cell receptors (TCR) for antigen. In T cell hybridizations, the AKR mouse strain-derived thymus lymphoma BW5147 is by far the most popular fusion line. However, the full potential of this technology had to await inactivation of the productively rearranged TCR- α and -β genes in the lymphoma. BWα-β-, the TCR-gene deficient variant of the original lymphoma, which has become the fusion line of choice for αβ T cells, is now available wi...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 18, 2020 Category: Allergy & Immunology Source Type: research

HLA-A and -B Type and Haplotype Frequencies in IgG Subclass Deficiency Subgroups
AbstractWe sought to determine whether HLA-A and -B type and haplotype frequencies differ between subgroups of adults with IgG subclass deficiency (IgGSD). We retrospectively compared type and haplotype frequencies of three subgroups of 269 unrelated adult IgGSD patients (70 subnormal IgG1; 121 subnormal IgG3; 78 subnormal IgG1/IgG3) and controls (1,321 for types; 751 for haplotypes). We selected types and haplotypes because their uncorrected frequencies differed significantly from controls in a previous adult IgGSD/common variable immunodeficiency cohort: A*24; B*14; B*35; B*40; B*49; B*50; B*58; B*62; A*01,B*08; A*02,B*4...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 20, 2020 Category: Allergy & Immunology Source Type: research

Crosstalk Between Immunity System Cells and Pancreas. Transformation of Stem Cells Used in the 3D Bioprinting Process as a Personalized Treatment Method for Type 1 Diabetes
AbstractInteractions between the immune system and the pancreas are pivotal in understanding how and why β cells’ damage causes problems with pancreas functioning. Pancreatic islets are crucial in maintaining glucose homeostasis in organs, tissue and cells. Autoimmune aggression towards pancreatic islets, mainly β cells, leads to type 1 diabetes—one of the most prevalent autoimmune disease in the world, being a worldwide risk to health of many people. In this review, we highlight the role of immune cells and its influence in the development of autoimmunity in Langerhans islets. Moreover, we discuss th...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 15, 2020 Category: Allergy & Immunology Source Type: research

Specific T-Cell Subsets Can Predict the Efficacy of Anti-TNF Treatment in Inflammatory Bowel Diseases
AbstractThe effect of TNF-blockers on T-lymphocyte subsets is largely unknown in inflammatory bowel diseases (IBDs). The aim of the present study was to analyze the prevalence of T-cell subtypes and their correlation to therapeutic response. Sixty-eight patients with Crohn ’s disease (CD), 46 with ulcerative colitis (UC) were enrolled. (1) The clinical course was followed after the initiation of TNF-blockers (prospective study). (2) The immunophenotype was also compared between long-term anti-TNF treated-responders and non-responders (cross-sectional study). The res ults were compared with those of therapy-naïve...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 4, 2020 Category: Allergy & Immunology Source Type: research

In Vitro Immunological Effects of CXCR3 Inhibitor AMG487 on Dendritic Cells
AbstractAMG 487 is the targeted blocker of chemokine receptor CXCR3 and improves inflammatory symptoms by blocking the inflammatory cycle. Here we investigated whether AMG 487 affects dendritic cell (DC) biology and function. The expression of co-stimulatory markers on DCs was reduced, indicating the semi-mature state of DC when AMG 487 was added throughout the in vitro differentiation period. Additionally, when added solely during the final lipopolysaccharide-induced activation step, AMG 487 inhibited DC activation, as demonstrated by a decreased expression of activation markers. AMG487 also promoted the expression of PD-...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 1, 2020 Category: Allergy & Immunology Source Type: research

Food Allergy Insights: A Changing Landscape
AbstractThe panorama of food allergies (FA) has changed profoundly in recent years. In light of recent advances in knowledge of pathogenetic mechanisms and a greater attention to the multifaceted range of possible clinical manifestations, there is a need for a critical review of past classifications. Changes in nutrition, environment and lifestyles around the world are modifying the global FA epidemiology and new FA phenotypes are also emerging. Furthermore, both biotechnological advances in this field and recent personalized therapies have improved the diagnostic and therapeutic approach to FA. Consequently, both the prev...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 1, 2020 Category: Allergy & Immunology Source Type: research

Current Status of M1 and M2 Macrophages Pathway as Drug Targets for Inflammatory Bowel Disease
This study reflects the novel IBD treatment targets via the immune system ’s pathway, reporting the latest treatments that regulate the M1/M2 macrophages distribution in a way to favor IBD. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - April 1, 2020 Category: Allergy & Immunology Source Type: research

Antibodies to Heat Shock Proteins 90 α and 90β in Psoriasis
AbstractOne of many hypotheses of psoriasis pathogenesis supposes an overexpression of heat shock proteins (Hsps) in different skin layers and systemic immunologic response to them. Hsp90 is one of the most abundant chaperone in eukaryotic cells. The number of studies concerning the role of Hsp90 and anti-Hsp90 antibodies in etiopathogenesis of various diseases is also constantly expanding. Still, there are not many reports concerning potential involvement of this Hsp family or anti-Hsp90 immunization in pathomechanism of psoriasis. The aim of the study was the estimation of anti-Hsp90 α and anti-Hsp90β IgG anti...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 1, 2020 Category: Allergy & Immunology Source Type: research

Correction to: Human Gyrovirus-Apoptin Interferes with the Cell Cycle and Induces G2/M Arrest Prior to Apoptosis
The authors would like to correct the following error. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - February 20, 2020 Category: Allergy & Immunology Source Type: research

Desloratadine Ameliorates Olfactory Disorder and Suppresses AMPA Receptor GluA1 Expression in Allergic Rhinitis Rat
AbstractAllergic rhinitis (AR) is an IgE-mediated inflammation which causes olfactory dysfunction. Antihistamines have been widely used to treat AR while few studies have investigated the effect of antihistamines on improving the sense of smell. In addition, the underlying mechanisms are not well elucidated. We established the ovalbumin (OVA)-induced allergic rhinitis rat model and administrated desloratadine to AR rats. The AR symptoms, serum level of OVA-specific IgE and IL-17, and expression of IL-4, IL-5 and IL-13 in nasal mucosa were measured. The olfactory dysfunction was monitored by buried food test and the express...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 19, 2020 Category: Allergy & Immunology Source Type: research

Autotransplantation of the Adipose Tissue-Derived Mesenchymal Stromal Cells in Therapy of Venous Stasis Ulcers
AbstractAdipose tissue is a reliable source of mesenchymal stromal cells (MSC) for use in regenerative medicine. The aim of this pilot study was to describe the method, and assess the safety and the potential efficacy of transplantation of autologous adipose tissue-derived MSC for the treatment of chronic venous stasis ulcers. Study group consisted of 11 patients (mean age: 66.6  ± 9.5 years) with chronic venous stasis ulcers. Adipose tissue was harvested by tumescent-aspiration method. Stromal cells were separated using a dedicated closed system in a real-time bedside manner. The phenotype of cel...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 14, 2020 Category: Allergy & Immunology Source Type: research

Can Interferon- γ Release Assays Be Useful for Monitoring the Response to Anti-tuberculosis Treatment?: A Systematic Review and Meta-analysis
AbstractThe number of studies which evaluated interferon-gamma release assays (IGRAs) results after anti-tuberculosis (TB) treatment has been rapidly increasing. The aim of this study was to investigate the potential use of IGRAs (QFT-GIT, T-SPOT.TB, QFT-Plus) in assessing the response to anti-TB treatment. We searched all studies in English language published from 1 October 2011 to 18 November 2018 in PubMed, Web of Science, and Scopus. Our search included the term “tuberculosis treatment AND interferon-γ release assay”. We included studies evaluating the performance of commercial IGRAs (including QFT-GI...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 3, 2020 Category: Allergy & Immunology Source Type: research

Treatment with Lycopodium clavatum 200dH Intensifies Kidney and Liver Injury in Mice Infected with Toxoplasma gondii
In this study, physiological, histopathological, and immunological parameters were evaluated. TheL. clavatum 200dH intensified renal damage in mice infected withT. gondii from 7 dpi, causing severe and progressive alterations during this period, such as various degrees of inflammation, edema, atrophy, and tubular cystic dilation, degenerated tubules with intra-cytoplasmic vacuoles and coalescing spots, severe vascular lesions, glomerulonephritis, and peri-glomerular congestion. In the G72 animals, which receivedL. clavatum 200dH, more severe cortex damage was observed (91.66 –96.66%) as compared to the IC group (55&n...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 21, 2020 Category: Allergy & Immunology Source Type: research

Type III Interferons (Lambda Interferons) in Rheumatic Autoimmune Diseases
AbstractThe last 2  decades have witnessed the discovery and characterization of a new family of cytokines with immunological characteristics similar to those described for type I interferons, type III or lambda interferons. Unraveling the molecular mechanisms underlying each type of interferon has allowed us to unde rstand how some autoimmune diseases can be considered as interferonopathies. Under normal conditions, type III interferons play a key role in the defense against viruses by modulating the functioning of several types of innate and adaptive immune cells. These effects include upregulation of major hi stoco...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 9, 2020 Category: Allergy & Immunology Source Type: research

HER2-Positive Breast Cancer Immunotherapy: A Focus on Vaccine Development
AbstractClinical progress in the field of HER2-positive breast cancer therapy has been dramatically improved by understanding of the immune regulatory mechanisms of tumor microenvironment. Passive immunotherapy utilizing recombinant monoclonal antibodies (mAbs), particularly trastuzumab and pertuzumab has proved to be an effective strategy in HER2-positive breast cancer treatment. However, resistance to mAb therapy and relapse of disease are still considered important challenges in clinical practice. There are increasing reports on the induction of cellular and humoral immune responses in HER2-positive breast cancer patien...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 9, 2020 Category: Allergy & Immunology Source Type: research

Modulatory Effect of the Euro-Lupus Low-Dose Intravenous Cyclophosphamide Regimen on Circulating Immune Cells in Systemic Lupus Erythematosus
AbstractA Euro-Lupus regimen of low-dose intravenous cyclophosphamide (CFA) is commonly used to treat severe organ manifestations of systemic lupus erythematosus (SLE), particularly lupus nephritis (LN). There are no data on the distributions and dynamics of immune cell populations in patients with various treatment outcomes. The circulating immune cells of 11 female SLE patients were assessed before and after Euro-Lupus regimen (cumulative dose of 3000  mg CFA) by flow cytometry together with those of 16 healthy women. A subanalysis was performed in LN patients who achieved complete remission(CR;n  =  3...
Source: Archivum Immunologiae et Therapiae Experimentalis - October 16, 2019 Category: Allergy & Immunology Source Type: research

Gut Microbiota in Neurological Disorders
AbstractThe incidence of neurological disorders such as multiple sclerosis (MS), Alzheimer ’s disease (AD) and Parkinson’s disease (PD) is increasing throughout the world, but their pathogenesis remains unclear and successful treatment remains elusive. Bidirectional communications between the central nervous system and gut microbiota may play some role in the pathogenesis of the above disorders. Up to a thousand bacterial species reside in human intestine; they colonize the gut shortly after birth and remain for life. Numerous studies point to the role of microbiota composition in the development, course and tr...
Source: Archivum Immunologiae et Therapiae Experimentalis - October 1, 2019 Category: Allergy & Immunology Source Type: research

Retinoids as an Immunity-modulator in Dermatology Disorders
AbstractThe skin is the largest epithelial surface protecting the body from invading microbes. Vitamin A plays vital roles in the host defence of the skin, including promoting epithelial cell integrity, proliferation, and differentiation and even mediating immune responses. Furthermore, vitamin A derivatives, retinoid drugs, are widely used to treat skin diseases, such as acne and psoriasis. However, the immunoregulatory mechanisms of retinoids in dermatology have not been systematically described. In this paper, we discuss the immunological functions of retinoids during disease treatment, especially in skin disorders caus...
Source: Archivum Immunologiae et Therapiae Experimentalis - September 24, 2019 Category: Allergy & Immunology Source Type: research

Peripheral Blood B and T Cell Profiles in Children with Active Juvenile Idiopathic Arthritis
In conclusion; JIA patients have an alteration in both B and T lymphocytes with the predisposition of memory cells which may have a role in sustaining the JIA disease activity. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - September 18, 2019 Category: Allergy & Immunology Source Type: research

Professor W łodzimierz Ptak (1928–2019)
(Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - September 13, 2019 Category: Allergy & Immunology Source Type: research

Correction to: Significance and Role of Pattern Recognition Receptors in Malignancy
The authors would like to correct the following error. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - August 31, 2019 Category: Allergy & Immunology Source Type: research

90th Birthday of Prof. Czes ław Radzikowski
(Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - August 30, 2019 Category: Allergy & Immunology Source Type: research

Clinical and Immunological Features of 78 Adult Patients with Primary Selective IgG Subclass Deficiencies
AbstractThe purpose of this study is to describe both clinical and immunological features in large cohort of adult patients with IgG subclass deficiency, and response to immunoglobulin therapy. This is a retrospective study of data obtained from electronic medical records and paper charts of 78 patients with IgG subclass deficiency seen and followed at our immunology clinics from 2010 to 2016. Both isolated selective IgG subclass deficiency as well as combined (two) subclass deficiencies were observed. IgG3 subclass deficiency, isolated and in combination with other IgG subclass deficiency, is the most frequent of IgG subc...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 30, 2019 Category: Allergy & Immunology Source Type: research

Interleukin-1 Genotype in Periodontitis
AbstractThis paper presents the current knowledge concerning the role of polymorphisms of IL1A and IL1B genes in periodontitis. Attention has been paid to the role of IL-1 in the pathogenesis of the disease, and to the significance of a genetic test, investigating the presence of composite two polymorphisms of IL-1 gene, as a risk factor for severe periodontitis. The significance of this test for prevention of periodontitis and its therapy has been discussed. IL-1 polymorphisms have been presented and described according to the reference single nucleotide polymorphism (SNP) identification number (rsID), established to erad...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 19, 2019 Category: Allergy & Immunology Source Type: research

Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts
AbstractPoxviruses utilize multiple strategies to prevent activation of extrinsic and intrinsic apoptotic pathways for successful replication. Mitochondrial heat shock proteins (mtHsps), especially Hsp60 and its cofactor Hsp10, are engaged in apoptosis regulation; however, until now, the influence of poxviruses on mtHsps has never been studied. We used highly infectious Moscow strain of ectromelia virus (ECTV) to investigate the mitochondrial heat shock response and apoptotic potential in permissive L929 fibroblasts. Our results show that ECTV-infected cells exhibit mostly mitochondrial localization of Hsp60 and Hsp10, and...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 19, 2019 Category: Allergy & Immunology Source Type: research

Role of Macrophages in Pregnancy and Related Complications
AbstractMacrophages (M Фs) are the leukocytes produced from differentiation of monocytes and are located in almost all tissues of human body. They are involved in various processes, such as phagocytosis, innate and adaptive immunity, proinflammatory (M1) and anti-inflammatory (M2) activity, depending on the tissue microe nvironment. They play a crucial role in pregnancy, and their dysfunction or alteration of polarity is involved in pregnancy disorders, like preeclampsia, recurrent spontaneous abortion, infertility, intrauterine growth restriction, and preterm labor. About 50–60% of decidual leukocytes are natural k...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 8, 2019 Category: Allergy & Immunology Source Type: research

Induction of Chronic Subclinical Systemic Inflammation in Sprague –Dawley Rats Stimulated by Intermittent Bolus Injection of Lipopolysaccharide
AbstractChronic subclinical systemic inflammation has a key role in stimulating several chronic conditions associated with cardiovascular diseases, cancer, rheumatoid arthritis, diabetes, and neurodegenerative diseases. Hence, developing in vivo models of chronic subclinical systemic inflammation are essential to the study of the pathophysiology and to measure the immunomodulatory agents involved. Male Sprague –Dawley rats were subjected to intraperitoneal, intermittent injection with saline, or lipopolysaccharide (LPS) (0.5, 1, 2 mg/kg) thrice a week for 30 days. Hematological, biochemical, and inflammator...
Source: Archivum Immunologiae et Therapiae Experimentalis - July 5, 2019 Category: Allergy & Immunology Source Type: research

Adjuvant Allergen Fusion Proteins as Novel Tools for the Treatment of Type I Allergies
AbstractWhile acute allergic symptoms can be managed by emergency medication, to date, allergen-specific immunotherapy (SIT) with allergen extracts is the only available curative treatment option. However, the risk of anaphylactic reactions, long treatment duration, varying extract quality, and underrepresentation of certain allergens currently prevent many patients from successfully undergoing SIT. Novel strategies are needed to enhance efficacy, safety, and convenience of allergy treatment. Fusion proteins combining allergen and adjuvant into a single molecule can efficiently induce immune responses by targeting the alle...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 20, 2019 Category: Allergy & Immunology Source Type: research

CTLA-4 Expression Inversely Correlates with Kidney Function and Serum Immunoglobulin Concentration in Patients with Primary Glomerulonephritides
The objective of this study was to determine the role of CTLA-4 in the pathogenesis of PGN and NPGN by evaluating the frequencies of T and B lymphocytes expressing CTLA-4 and the serum concentration of the sCTLA-4 isoform in patients with PGN and NPGN in relation to clinical parameters. The study included peripheral blood (PB) samples from 40 PGN and NPGN patients and 20 healthy age- and sex-matched volunteers (control group). The viable PB lymphocytes were labeled with fluorochrome-conjugated monoclonal anti-CTLA-4 antibodies and analyzed using flow cytometry. The serum concentration of sCTLA-4 was measured using ELISA. T...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 8, 2019 Category: Allergy & Immunology Source Type: research

Diverse Activity of IL-17 + Cells in Chronic Skin and Mucosa Graft-Versus-Host Disease
This study focused on the identification of IL-17-producing cells in the tissue biopsies of cGvHD patients. Forty-one biopsy specimens of cGvHD lesions of the skin (n  =  27), gastrointestinal tract (n  =  9) and oral mucosa (n  =  5), examined in 24 patients, were morphologically defined according to the NIH criteria and analyzed for the presence of cellular infiltrations including: IL-17+, FOXP3+ and CCR6+ cells. IL-17+ cells were identified in 26/27 skin and in all gut and oral mucosa biopsies, being more frequent in mucosa lesions than in the skin (11/14 vs 14/26, respectively;...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 8, 2019 Category: Allergy & Immunology Source Type: research

Variant and Specific Forms of Autoimmune Cholestatic Liver Diseases
AbstractPrimary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are the main autoimmune cholestatic liver diseases. IgG4-associated sclerosing cholangitis is another distinct immune-mediated cholestatic disorder of unknown aetiology that is frequently associated with autoimmune pancreatitis or other IgG4-related diseases. Although the majority of PBC and PSC patients have a typical presentation, there are common and uncommon important variants or specific subgroups that observed in everyday routine clinical practice. In this updated review, we summarize the published data giving also our own experience o...
Source: Archivum Immunologiae et Therapiae Experimentalis - June 5, 2019 Category: Allergy & Immunology Source Type: research

Proportion of Cytotoxic Peripheral Blood Natural Killer Cells and T-Cell Large Granular Lymphocytes in Recurrent Miscarriage and Repeated Implantation Failure: Case –Control Study and Meta-analysis
AbstractWe aimed to compare the proportion of peripheral blood natural killer (NK) cells (CD3–CD56+) and T-cell large granular lymphocytes (CD8+CD57+) during preconception in a homogenous group of women with unexplained well-defined recurrent miscarriage (RM) and repeated implantation failure (RIF) vs healthy controls in relation to pregnancy outcomes. This case –control study followed by a literature review and meta-analysis was conducted in three university hospitals. Patients and controls were consecutively recruited from December 2015 to October 2017. In total, 115 women were included in the study: 54 with ...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 30, 2019 Category: Allergy & Immunology Source Type: research

Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice
AbstractVisfatin is involved in the body ’s inflammation and immune response. Inflammation could promote, while visfatin may directly or indirectly mitigate the effects of apoptosis and autophagy. Whether visfatin lessens the detrimental effects of lipopolysaccharide (LPS)-induced mouse acute lung injury (ALI) is poorly understood yet. T herefore, in the current study, the regulation mechanism of visfatin on apoptosis and autophagy was explored in Kunming mice by replicating LPS-induced inflammatory ALI model. Based on the mouse model of ALI, HE staining, TUNEL, transmission electron microscopy, immunohistochemical s...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 29, 2019 Category: Allergy & Immunology Source Type: research

MicroRNAs: Potential Biomarkers and Targets of Therapy in Allergic Diseases?
AbstractMicroRNAs (miRNAs) are small non-coding RNA molecules that are 18 –22 nucleotides long and highly conserved throughout evolution. Currently, they are considered one of the fundamental regulatory mechanisms of genes expression. It has been demonstrated that miRNAs are involved in many biologic processes, such as signal transduction, cell proliferation and differe ntiation, apoptosis and stress responses. More recently, the role of miRNA has also been revealed in numerous immunological and inflammatory disorders, including allergic inflammation. Specific miRNA profiles were demonstrated in asthma, allergic rhin...
Source: Archivum Immunologiae et Therapiae Experimentalis - May 28, 2019 Category: Allergy & Immunology Source Type: research

IgG from Non-atopic Individuals Induces In Vitro IFN- γ and IL-10 Production by Human Intra-thymic γδT Cells: A Comparison with Atopic IgG and IVIg
This report describes evidence revealing that IgG may influence the production of IFN-γ and IL-10 by intra thymic γδT cells depending on the donor atopic state. This observation is unprecedented and needs to be considered in further studies in the IgG immunotherapy field. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - May 13, 2019 Category: Allergy & Immunology Source Type: research

The Influence of Antidepressants on the Immune System
AbstractDepression is one of the most frequently diagnosed condition in psychiatry. Despite the availability of many preparations, over 30% of treated patients do not achieve remission. Recently the emphasis is put on the contribution of the body ’s inflammatory response as one of the causes of depression. The interactions between nervous and immune systems are the main issue addressed by psychoneuroimmunology. In patients suffering from depression changes in the plasma concentrations of cytokines and in the number and level of activation of immune cells has been found. Attention is paid to the high levels of pro-inf...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 29, 2019 Category: Allergy & Immunology Source Type: research

Conjugation of Meningococcal Lipooligosaccharides Through Their Non-Reducing Terminus Results in Improved Induction a Protective Immune Response
AbstractThe present studies prove that conjugation of meningococcal lipooligosaccharides through their non-reducing terminus conserves their inner epitopes resulting in conjugates potent to induce a protective immune response. Four different oligosaccharides were obtained by specific degradations of the same L7 lipooligosaccharide (L7-LOS), and each was linked to tetanus toxoid by direct reductive amination. Two were truncated oligosaccharides with incomplete inner epitopes and were obtained by mild acid hydrolysis of lipooligosaccharide. The terminal galactose of one oligosaccharide was additionally enzymatically oxidized...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 27, 2019 Category: Allergy & Immunology Source Type: research

Post-transplant Alternative Complement Pathway Activation Influences Kidney Allograft Function
AbstractThe complement system is one of the crucial pathophysiological mechanisms that directly influence the function of a transplanted kidney. Since the complement pathways ’ activation potential can be easily determined via their functional activity measurement, we focused on fluctuation in the cascade activity in the early post-transplant period. The aim of the study was to relate the kidney transplantation-induced complement system response to allograft outcome. F orty-two kidney recipients (aged: 53.5 [37–52], 17 females/25 males) and 24 healthy controls (aged: 40.5 [34–51], 13 females/11 males) wer...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 26, 2019 Category: Allergy & Immunology Source Type: research

Heterogeneous Mixture of Amniotic Cells is Likely a Better Source of Stem Cells than Adipose Tissue
AbstractStem cells are increasingly being used in the course of burn treatment. As several different types of stem cells are available for the purposes, it is important to chose the most efficient and the most practicable stem cell type. The aim of this study was to compare the potential of heterogeneous amnion cell mixture with the presently used standard therapy, the adipose tissue-derived stem cells. The placenta was collected during a Cesarean section procedure. Adipose tissue tissue-derived cells were isolated using the Cytori ’s Celution® System. Cells were tested for fulfillment of the minimum criteria for...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 16, 2019 Category: Allergy & Immunology Source Type: research

Significance and Role of Pattern Recognition Receptors in Malignancy
AbstractPattern recognition receptors (PRRs) are members of innate immunity, playing pivotal role in several immunological reactions. They are known to act as a bridge between innate and adaptive immunity. They are expressed on several normal cell types but have been shown with increasing frequency on/in tumor cells. Significance of this phenomenon is largely unknown, but it has been shown by several authors that they, predominantly Toll-like receptors (TLRs), act in the interest of tumor, by promotion of its growth and spreading. Preparation of artificial of TLRs ligands (agonists) paved the way to use them as a therapeut...
Source: Archivum Immunologiae et Therapiae Experimentalis - April 11, 2019 Category: Allergy & Immunology Source Type: research

Natural Killer and Natural Killer T Cells in Juvenile Systemic Lupus Erythematosus: Relation to Disease Activity and Progression
In conclusion, low frequency of NK and NKT and expansion of CD56bright NK cells are marked in juvenile SLE, particularly with activity. These changes have direct effect on renal impairment and growth failure, reflecting their potential influence on disease progression. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - April 4, 2019 Category: Allergy & Immunology Source Type: research

PD-L1 Ameliorates Murine Acute Graft-Versus-Host Disease by Suppressing Effector But Not Regulatory T Cells Function
In this study, we carried out studies in a murine aGVHD model to clarify the role of PD-L1 in aGVHD pathogenesis. We found that systemic overexpression of PD-L1 by hydrodynamic gene transfer (HGT) method in vivo ameliorates aGVHD-induced lethality in mice. Systemic overexpression of PD-L1 inhibits the donor T cells activation, effector memory status, as well as Th1 and Th17 cells responses in vivo. In addition, PD-L1 Ig treatment significantly suppressed T cells ’ proliferation, promoted T cells’ apoptosis, and reduced pro-inflammatory cytokines expression by effector T cells in vitro in the stimulation of anti...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 29, 2019 Category: Allergy & Immunology Source Type: research

Theaflavin-3, 3 ′-Digallate Attenuates Rheumatoid Inflammation in Mice Through the Nuclear Factor-κB and MAPK Pathways
This study aimed to explore the attenuation of TFDG on RA. The collagen-induced arthritis (CIA) mouse model was established and administered with TFDG. The arthritis score and incidence was recorded to assess the ameli oration of TFDG on arthritis. Histopathological change of the mouse joint tissues was evaluated by haemotoxylin and eosin staining. The expression of pro-inflammatory mediators including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 was quantified by ELISA. The activation of nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signaling pathways in the synovium w...
Source: Archivum Immunologiae et Therapiae Experimentalis - March 14, 2019 Category: Allergy & Immunology Source Type: research

The Expression of the SLIT –ROBO Family in Adult Patients with Acute Myeloid Leukemia
AbstractIntroductionSLIT –ROBO is a ligand–receptor family of neuronal guidance cues that has been involved in pathological and physiological angiogenesis. SLIT–ROBO expression is altered in many tumours. However, no data exist about the role of the whole family in acute myelogenous myeloid leukemia (AML).PurposeHerein, we assessed the expression of all SLIT –ROBO family in bone marrow (BM) biopsy of AML patients and control group on both protein and RNA levels.MethodsThe paraffin-embedded tissue blocks were subjected to immunohistochemistry for SLIT1, SLIT2, SLIT3, ROBO1, ROBO2, ROBO3, and ROBO4. M...
Source: Archivum Immunologiae et Therapiae Experimentalis - February 28, 2019 Category: Allergy & Immunology Source Type: research

The Effect of Fucoidan, a Potential New, Natural, Anti-Neoplastic Agent on Uterine Sarcomas and Carcinosarcoma Cell Lines: ENITEC Collaborative Study
AbstractThe aim of the study was to assess the activity of fucoidan on the uterine sarcomas (MES-SA and ESS-1) and carcinosarcoma cell lines (SK-UT-1 and SK-UT-1B) and its toxicity on the human skin fibroblasts (HSF). Two uterine sarcomas and two carcinosarcoma cell lines were examined, as a control HSF were used. Cell viability was assessed with MTT test, apoptosis with caspase-3 activity and cell cycle by assessment of DNA synthesis. Fucoidan significantly decreases cell viability in SK-UT-1, SK-UT-1B, and ESS-1 cell lines, such effect was not observed in MES-SA. Fucoidan was not substantially affecting proliferation amo...
Source: Archivum Immunologiae et Therapiae Experimentalis - January 18, 2019 Category: Allergy & Immunology Source Type: research

Correction to: Building the Prestige of Archivum Immunologiae et Therapiae Experimentalis : From a Little Known to an Internationally Recognized Journal
According to JCR data, updated in October 2018 by Clarivate Analytics. (Source: Archivum Immunologiae et Therapiae Experimentalis)
Source: Archivum Immunologiae et Therapiae Experimentalis - December 7, 2018 Category: Allergy & Immunology Source Type: research

Modulation of the Immune System in Chronic Hepatitis C and During Antiviral Interferon-Free Therapy
AbstractThe treatment of patients with chronic hepatitis C virus (HCV) infection has changed tremendously over the past 2  years, with an increasing variety of all-oral direct-acting antiviral (DAA) treatment regimens available for different HCV genotypes and distinct clinical settings. These treatments have significantly improved safety in patients with advanced liver disease compared with interferon (IFN)-based regi mens. HCV modifies the human immune system to escape immunosurveillance via several mechanisms. One of the basic mechanisms of HCV is the ability to “switch” the immune response by reducing t...
Source: Archivum Immunologiae et Therapiae Experimentalis - November 15, 2018 Category: Allergy & Immunology Source Type: research