Prolidase activity assays. A survey of the reported literature methodologies
Anal Biochem. 2024 Mar 7:115506. doi: 10.1016/j.ab.2024.115506. Online ahead of print.ABSTRACTProlidase (EC.3.4.13.9) is a dipeptidase known nowadays to play a pivotal role in several physiological and pathological processes. More in particular, this enzyme is involved in the cleavage of proline- and hydroxyproline-containing dipeptides (imidodipeptides), thus finely regulating the homeostasis of free proline and hydroxyproline. Abnormally high or low levels of prolidase have been found in numerous acute and chronic syndromes affecting humans (chronic liver fibrosis, viral and acute hepatitis, cancer, neurological disorder...
Source: Analytical Biochemistry - March 9, 2024 Category: Biochemistry Authors: Serena Fiorito Salvatore Genovese Francesco Epifano Chiara Collevecchio Source Type: research
Clinical relevance and advances in detection of translational biomarker cardiac troponin
Anal Biochem. 2024 Mar 7:115505. doi: 10.1016/j.ab.2024.115505. Online ahead of print.ABSTRACTCardiovascular diseases (CVD) are a range of diseases, pointing the functional hindrances in the heart and blood vessels of the human system that contributes to 48.6 % of the world's adult death rate. The diagnosis of CVD relies upon the Electro Cardio Gram (ECG) and detection of muscle markers such as troponins. Among the cardiac trio, Cardiac Troponin I (cTnI) weighing 23 KiloDalton (kDa) is a sorted biomarker for CVD. cTnI remains high in the blood after 1-2 weeks of myocardial damage. Testing of cTnI in CVD patients aids in di...
Source: Analytical Biochemistry - March 9, 2024 Category: Biochemistry Authors: Brindha Durairaj S Mohandass Kunnathur Murugesan Sakthivel Arthanareshwarar Arunachalam Poornima Source Type: research
MMDB: Multimodal dual-branch model for multi-functional bioactive peptide prediction
Anal Biochem. 2024 Mar 7:115491. doi: 10.1016/j.ab.2024.115491. Online ahead of print.ABSTRACTBioactive peptides can hinder oxidative processes and microbial spoilage in foodstuffs and play important roles in treating diverse diseases and disorders. While most of the methods focus on single-functional bioactive peptides and have obtained promising prediction performance, it is still a significant challenge to accurately detect complex and diverse functions simultaneously with the quick increase of multi-functional bioactive peptides. In contrast to previous research on multi-functional bioactive peptide prediction based so...
Source: Analytical Biochemistry - March 9, 2024 Category: Biochemistry Authors: Yan Kang Huadong Zhang Xinchao Wang Yun Yang Qi Jia Source Type: research
A novel ACE2-Based electrochemical biosensor for sensitive detection of SARS-CoV-2
Anal Biochem. 2024 Mar 6;689:115504. doi: 10.1016/j.ab.2024.115504. Online ahead of print.ABSTRACTSARS-CoV-2 emerged in late 2019 and quickly spread globally, resulting in significant morbidity, mortality, and socio-economic disruptions. As of now, collaborative global efforts in vaccination and the advent of novel diagnostic tools have considerably curbed the spread and impact of the virus in many regions. Despite this progress, the demand remains for low-cost, accurate, rapid and scalable diagnostic tools to reduce the influence of SARS-CoV-2. Herein, the angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2,...
Source: Analytical Biochemistry - March 8, 2024 Category: Biochemistry Authors: Hamidreza Ghaedamini Khalid Khalaf Dong-Shik Kim Yuan Tang Source Type: research
4 mC site recognition algorithm based on pruned pre-trained DNABert-Pruning model and fused artificial feature encoding
Anal Biochem. 2024 Mar 6:115492. doi: 10.1016/j.ab.2024.115492. Online ahead of print.ABSTRACTDNA 4 mC plays a crucial role in the genetic expression process of organisms. However, existing deep learning algorithms have shortcomings in the ability to represent DNA sequence features. In this paper, we propose a 4 mC site identification algorithm, DNABert-4mC, based on a fusion of the pruned pre-training DNABert-Pruning model and artificial feature encoding to identify 4 mC sites. The algorithm prunes and compresses the DNABert model, resulting in the pruned pre-training model DNABert-Pruning. This model reduces the number o...
Source: Analytical Biochemistry - March 8, 2024 Category: Biochemistry Authors: Guo-Bo Xie Yi Yu Zhi-Yi Lin Rui-Bin Chen Jian-Hui Xie Zhen-Guo Liu Source Type: research
A novel ACE2-Based electrochemical biosensor for sensitive detection of SARS-cov-2
Anal Biochem. 2024 Mar 6:115504. doi: 10.1016/j.ab.2024.115504. Online ahead of print.ABSTRACTSARS-CoV-2 emerged in late 2019 and quickly spread globally, resulting in significant morbidity, mortality, and socio-economic disruptions. As of now, collaborative global efforts in vaccination and the advent of novel diagnostic tools have considerably curbed the spread and impact of the virus in many regions. Despite this progress, the demand remains for low-cost, accurate, rapid and scalable diagnostic tools to reduce the influence of SARS-CoV-2. Herein, the angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2, was...
Source: Analytical Biochemistry - March 8, 2024 Category: Biochemistry Authors: Hamidreza Ghaedamini Khalid Khalaf Dong-Shik Kim Yuan Tang Source Type: research
4 mC site recognition algorithm based on pruned pre-trained DNABert-Pruning model and fused artificial feature encoding
Anal Biochem. 2024 Mar 6:115492. doi: 10.1016/j.ab.2024.115492. Online ahead of print.ABSTRACTDNA 4 mC plays a crucial role in the genetic expression process of organisms. However, existing deep learning algorithms have shortcomings in the ability to represent DNA sequence features. In this paper, we propose a 4 mC site identification algorithm, DNABert-4mC, based on a fusion of the pruned pre-training DNABert-Pruning model and artificial feature encoding to identify 4 mC sites. The algorithm prunes and compresses the DNABert model, resulting in the pruned pre-training model DNABert-Pruning. This model reduces the number o...
Source: Analytical Biochemistry - March 8, 2024 Category: Biochemistry Authors: Guo-Bo Xie Yi Yu Zhi-Yi Lin Rui-Bin Chen Jian-Hui Xie Zhen-Guo Liu Source Type: research
Rapid diagnosis of acute myocardial infarction through integrated microfluidic chips for detection of characteristic targets
Anal Biochem. 2024 Mar 5:115502. doi: 10.1016/j.ab.2024.115502. Online ahead of print.ABSTRACTMyoglobin (Myo), creatine kinase-MB (CKMB), and cardiac troponin I (cTnI) are crucial biomarkers for diagnosing acute myocardial infarction (AMI) The accurate and rapid detection of these three targets can greatly improve the prognosis of AMI patients. Herein, this study developed a microfluidic immunofluorescence method that can detect all three targets in 10-15 minutes. Ultrasonic atomization and spray technology are used to modify the surface of the injection-molded microfluidic chip (MFC), which effectively solves the problem ...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Yaling Yuan Yue Zhong Yanbin Yang Ke Li Source Type: research
A comparative analysis of univariate versus multivariate eco-friendly spectrophotometric manipulations for resolving severely overlapped spectra of vonoprazan and amoxicillin new combination
Anal Biochem. 2024 Mar 5:115501. doi: 10.1016/j.ab.2024.115501. Online ahead of print.ABSTRACTVonoprazan and amoxicillin are pharmacological combinations that demonstrate synergistic effects in treating Helicobacter pylori (H. pylori), a global public health concern associated with peptic ulcer disease and gastric cancer. Four spectrophotometric methods were developed, including two univariate techniques (Fourier self-deconvolution and ratio difference) and two multivariate chemometric approaches (partial least squares and principal component regression). These methods provide innovative solutions for effectively resolving...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Ahmed K Kammoun Hani M Hafez Ebraam B Kamel Michael Gamal Fawzy Source Type: research
Quantifying terpenes in tomato leaf extracts from different species using gas chromatography-mass spectrometry (GC-MS)
This study employed two distinct methods, namely leaf dip and maceration, to extract trichomes from tomato leaves. Terpene quantification was carried out using Gas Chromatography-Mass Spectrometry (GC-MS). The leaf dip method proved effective in selectively targeting trichome content, revealing unique extraction patterns compared to maceration. The GC-MS method demonstrated high linearity, accuracy, sensitivity, and low limits of detection and quantification. Application of the method to different tomato species (Solanum pennellii, Solanum pimpinellifolium, Solanum galapagense, Solanum habrochaites, and Solanum lycopersicu...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Jessica S Pizzo Thiago Rutz Ann S Ojeda Kimberly Y Kartowikromo Ahmed M Hamid Alvin Simmons Andre L B R da Silva Camila Rodrigues Source Type: research
Rapid diagnosis of acute myocardial infarction through integrated microfluidic chips for detection of characteristic targets
Anal Biochem. 2024 Mar 5:115502. doi: 10.1016/j.ab.2024.115502. Online ahead of print.ABSTRACTMyoglobin (Myo), creatine kinase-MB (CKMB), and cardiac troponin I (cTnI) are crucial biomarkers for diagnosing acute myocardial infarction (AMI) The accurate and rapid detection of these three targets can greatly improve the prognosis of AMI patients. Herein, this study developed a microfluidic immunofluorescence method that can detect all three targets in 10-15 minutes. Ultrasonic atomization and spray technology are used to modify the surface of the injection-molded microfluidic chip (MFC), which effectively solves the problem ...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Yaling Yuan Yue Zhong Yanbin Yang Ke Li Source Type: research
A comparative analysis of univariate versus multivariate eco-friendly spectrophotometric manipulations for resolving severely overlapped spectra of vonoprazan and amoxicillin new combination
Anal Biochem. 2024 Mar 5:115501. doi: 10.1016/j.ab.2024.115501. Online ahead of print.ABSTRACTVonoprazan and amoxicillin are pharmacological combinations that demonstrate synergistic effects in treating Helicobacter pylori (H. pylori), a global public health concern associated with peptic ulcer disease and gastric cancer. Four spectrophotometric methods were developed, including two univariate techniques (Fourier self-deconvolution and ratio difference) and two multivariate chemometric approaches (partial least squares and principal component regression). These methods provide innovative solutions for effectively resolving...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Ahmed K Kammoun Hani M Hafez Ebraam B Kamel Michael Gamal Fawzy Source Type: research
Quantifying terpenes in tomato leaf extracts from different species using gas chromatography-mass spectrometry (GC-MS)
This study employed two distinct methods, namely leaf dip and maceration, to extract trichomes from tomato leaves. Terpene quantification was carried out using Gas Chromatography-Mass Spectrometry (GC-MS). The leaf dip method proved effective in selectively targeting trichome content, revealing unique extraction patterns compared to maceration. The GC-MS method demonstrated high linearity, accuracy, sensitivity, and low limits of detection and quantification. Application of the method to different tomato species (Solanum pennellii, Solanum pimpinellifolium, Solanum galapagense, Solanum habrochaites, and Solanum lycopersicu...
Source: Analytical Biochemistry - March 7, 2024 Category: Biochemistry Authors: Jessica S Pizzo Thiago Rutz Ann S Ojeda Kimberly Y Kartowikromo Ahmed M Hamid Alvin Simmons Andre L B R da Silva Camila Rodrigues Source Type: research
COVID-19 electrochemical immunosensor with Ag-MOF: Rapid and high-selectivity nasal swab testing for effective detection
In this study, we leveraged the properties of synthesized Ag-MOF, including high porosity and increased flow intensity. Electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to develop an economical and portable sensor with exceptional selectivity for COVID-19 detection. The methodology involves the deposition of Ag-MOF onto the surface of a Glassy Carbon Electrode (GCE), which resulted in a progressive augmentation of electric current. Subsequently, the targeted antibodies were applied, and relevant tests were conducted. The sensor demonstrated the capacity to de...
Source: Analytical Biochemistry - March 2, 2024 Category: Biochemistry Authors: Sadra Adel Ali Firoozbakhtian Hodjattallah Rabbani Morteza Hosseini Azam Bagheri Pebdeni Niloufar Sadeghi Javad Gilnezhad Mohammad Reza Ganjali Source Type: research
An LC-MS/MS-based method for the simultaneous quantification of melatonin, cortisol and cortisone in saliva
Anal Biochem. 2024 Feb 29:115496. doi: 10.1016/j.ab.2024.115496. Online ahead of print.ABSTRACTDisturbances in the diurnal pattern are associated with several clinical and psychological conditions, including depression and fatigue. Salivary sampling for melatonin, cortisol and cortisone provides a non-invasive method for frequent sampling and obtaining biochemical insight into the diurnal pattern of individuals. Therefore, a new liquid chromatography-tandem mass spectrometry-based method for the measurement of salivary melatonin, cortisol and cortisone was developed and validated. The method required 250 μl saliva, used i...
Source: Analytical Biochemistry - March 2, 2024 Category: Biochemistry Authors: Mirthe Lanfermeijer Lennart J van Winden Danielle E J Starreveld Serry Razab-Sekh Martijn van Faassen Eveline M A Bleiker Huub H van Rossum Source Type: research
