Overview of the different methods for RNA preparation in COVID-19 diagnosis process during the pandemic
Anal Biochem. 2023 Nov 23:115410. doi: 10.1016/j.ab.2023.115410. Online ahead of print.ABSTRACTThe COVID-19 pandemic brought to light the impact of a widespread disease on various aspects of human relationships, communities, and economies. One notable consequence was the increased demand for diagnostic kits, laboratory reagents, and personal health equipment. This surge in testing capacity worldwide led to shortages in the supply of essential items, including RNA extraction kits, which are crucial for detecting COVID-19 infections. To address this scarcity, researchers have proposed alternative and cost-effective strategie...
Source: Analytical Biochemistry - November 25, 2023 Category: Biochemistry Authors: Fatemeh Shahi Mojtaba Rasti Melika Moradi Source Type: research
Comparing in vitro affinity measurements of antibodies to TfR1: Surface plasmon resonance versus on-cell affinity
In conclusion, it is important to evaluate assay parameters including assay orientation, surface capture method, and antibody-format when comparing binding kinetics for TfR1 antibodies. Although it seems possible to determine relative affinities of TfR1 antibodies using the methods described here, both the FLAG-tag TfR1 capture setup and cell assays likely yield apparent affinities that are most translatable in vivo.PMID:38006952 | DOI:10.1016/j.ab.2023.115406 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - November 25, 2023 Category: Biochemistry Authors: Gillian Bonvicini Sunitha Singh Patrik Nygren Mengfei Xiong Stina Syv änen Dag Sehlin Ronny Falk Ken G Andersson Source Type: research
Characterization of an ssDNA ligase and its application in aptamer circularization
This study provides a simple and efficient aptamer circularization modification method for improving aptamer stability and expanding its applications.PMID:38006953 | DOI:10.1016/j.ab.2023.115409 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - November 25, 2023 Category: Biochemistry Authors: Zhenxia Ma Han Chen Yao Yang Siyi Gao Jiaping Yang Shihai Cui Shiyuan Zhou Boyang Jiang Bin Zou Mingjuan Sun Lianghua Wang Source Type: research
Recent progress in MoS < sub > 2 < /sub > nanostructures for biomedical applications: Experimental and computational approach
Anal Biochem. 2023 Nov 20:115404. doi: 10.1016/j.ab.2023.115404. Online ahead of print.ABSTRACTIn the category of 2D materials, MoS2 a transition metal dichalcogenide, is a novel and intriguing class of materials with interesting physicochemical properties, explored in applications ranging from cutting-edge optoelectronic to the frontiers of biomedical and biotechnology. MoS2 nanostructures an alternative to heavy toxic metals exhibit biocompatibility, low toxicity and high stability, and high binding affinity to biomolecules. MoS2 nanostructures provide a lot of opportunities for the advancement of novel biosensing, nanod...
Source: Analytical Biochemistry - November 22, 2023 Category: Biochemistry Authors: Shivani Bharti S K Tripathi Kedar Singh Source Type: research
Recent progress in MoS < sub > 2 < /sub > nanostructures for biomedical applications: Experimental and computational approach
Anal Biochem. 2023 Nov 20:115404. doi: 10.1016/j.ab.2023.115404. Online ahead of print.ABSTRACTIn the category of 2D materials, MoS2 a transition metal dichalcogenide, is a novel and intriguing class of materials with interesting physicochemical properties, explored in applications ranging from cutting-edge optoelectronic to the frontiers of biomedical and biotechnology. MoS2 nanostructures an alternative to heavy toxic metals exhibit biocompatibility, low toxicity and high stability, and high binding affinity to biomolecules. MoS2 nanostructures provide a lot of opportunities for the advancement of novel biosensing, nanod...
Source: Analytical Biochemistry - November 22, 2023 Category: Biochemistry Authors: Shivani Bharti S K Tripathi Kedar Singh Source Type: research
Recent progress in MoS < sub > 2 < /sub > nanostructures for biomedical applications: Experimental and computational approach
Anal Biochem. 2023 Nov 20:115404. doi: 10.1016/j.ab.2023.115404. Online ahead of print.ABSTRACTIn the category of 2D materials, MoS2 a transition metal dichalcogenide, is a novel and intriguing class of materials with interesting physicochemical properties, explored in applications ranging from cutting-edge optoelectronic to the frontiers of biomedical and biotechnology. MoS2 nanostructures an alternative to heavy toxic metals exhibit biocompatibility, low toxicity and high stability, and high binding affinity to biomolecules. MoS2 nanostructures provide a lot of opportunities for the advancement of novel biosensing, nanod...
Source: Analytical Biochemistry - November 22, 2023 Category: Biochemistry Authors: Shivani Bharti S K Tripathi Kedar Singh Source Type: research
A novel fusion technology utilizing complex network and sequence information for FAD-binding site identification
Anal Biochem. 2023 Nov 20;685:115401. doi: 10.1016/j.ab.2023.115401. Online ahead of print.ABSTRACTFlavin adenine dinucleotide (FAD) binding sites play an increasingly important role as useful targets for inhibiting bacterial infections. To reveal protein topological structural information as a reasonable complement for the identification FAD-binding sites, we designed a novel fusion technology according to sequence and complex network. The specially designed feature vectors were combined and fed into CatBoost for model construction. Moreover, due to the minority class (positive samples) is more significant for biological ...
Source: Analytical Biochemistry - November 19, 2023 Category: Biochemistry Authors: Lichao Zhang Kang Xiao Xueting Wang Liang Kong Source Type: research
A novel fusion technology utilizing complex network and sequence information for FAD-binding site identification
Anal Biochem. 2023 Nov 17:115401. doi: 10.1016/j.ab.2023.115401. Online ahead of print.ABSTRACTFlavin adenine dinucleotide (FAD) binding sites play an increasingly important role as useful targets for inhibiting bacterial infections. To reveal protein topological structural information as a reasonable complement for the identification FAD-binding sites, we designed a novel fusion technology according to sequence and complex network. The specially designed feature vectors were combined and fed into CatBoost for model construction. Moreover, due to the minority class (positive samples) is more significant for biological rese...
Source: Analytical Biochemistry - November 19, 2023 Category: Biochemistry Authors: Lichao Zhang Kang Xiao Xueting Wang Liang Kong Source Type: research
Generation of autoantibodies against glycated fibrinogen: Role in diabetic nephropathy and retinopathy
This study not only establishes the prevalence of autoantibodies against d-ribose glycated fibrinogen in DN but also highlights the potential of glycated fibrinogen as a biomarker for the detection of DN and/or DR. These insights may open new avenues for research into novel therapeutic strategies and the prevention of diabetes-related nephropathy and retinopathy.PMID:37977213 | DOI:10.1016/j.ab.2023.115393 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - November 17, 2023 Category: Biochemistry Authors: Afreen Khanam Sultan Alouffi Amjad R Alyahyawi Arbab Husain Saif Khan Talal Alharazi Rihab Akasha Hamda Khan Uzma Shahab Saheem Ahmad Source Type: research
Investigation of the electrochemical properties of edoxaban using glassy carbon and boron-doped diamond electrodes and development of an eco-friendly and cost effective voltammetric method for its determination
In this study, the highly risky drug Edoxaban (EDX), which can threaten life and cause bleeding, was electro analytically evaluated. The electrochemical behavior of EDX was investigated using glassy carbon electrode (GCE) and boron-doped diamond electrode (BDDE). In this study, for the first time, a simple, rapid, sensitive, and selective voltammetric technique was developed by using different electrodes for the electrochemical characterization and detection of EDX. The optimized voltammetric technique showed anodic signals of EDX at +1.09 V and +1.08 V on GCE and BDDE, respectively, in BR (pH 5.0) solution. The developed ...
Source: Analytical Biochemistry - November 17, 2023 Category: Biochemistry Authors: Abdulkadir Kili ç Mehmet Aslan Abdulkadir Levent Source Type: research
Generation of autoantibodies against glycated fibrinogen: Role in diabetic nephropathy and retinopathy
This study not only establishes the prevalence of autoantibodies against d-ribose glycated fibrinogen in DN but also highlights the potential of glycated fibrinogen as a biomarker for the detection of DN and/or DR. These insights may open new avenues for research into novel therapeutic strategies and the prevention of diabetes-related nephropathy and retinopathy.PMID:37977213 | DOI:10.1016/j.ab.2023.115393 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - November 17, 2023 Category: Biochemistry Authors: Afreen Khanam Sultan Alouffi Amjad R Alyahyawi Arbab Husain Saif Khan Talal Alharazi Rihab Akasha Hamda Khan Uzma Shahab Saheem Ahmad Source Type: research
Investigation of the electrochemical properties of edoxaban using glassy carbon and boron-doped diamond electrodes and development of an eco-friendly and cost effective voltammetric method for its determination
In this study, the highly risky drug Edoxaban (EDX), which can threaten life and cause bleeding, was electro analytically evaluated. The electrochemical behavior of EDX was investigated using glassy carbon electrode (GCE) and boron-doped diamond electrode (BDDE). In this study, for the first time, a simple, rapid, sensitive, and selective voltammetric technique was developed by using different electrodes for the electrochemical characterization and detection of EDX. The optimized voltammetric technique showed anodic signals of EDX at +1.09 V and +1.08 V on GCE and BDDE, respectively, in BR (pH 5.0) solution. The developed ...
Source: Analytical Biochemistry - November 17, 2023 Category: Biochemistry Authors: Abdulkadir Kili ç Mehmet Aslan Abdulkadir Levent Source Type: research
Discriminative detection of soman or VX exposure using europium chelated microparticle-based immunofluorescence microfluidic chip
Anal Biochem. 2023 Nov 13:115388. doi: 10.1016/j.ab.2023.115388. Online ahead of print.ABSTRACTThe retrospective detection of organophosphorus nerve agents (OPNAs) exposure has been achieved by the off-site analysis of OPNA-human serum albumin (HSA) adducts using mass spectrometry-based detection approaches. However, few specific methods are accessible for on-site detection. To address this, a novel immunofluorescence microfluidic chip (IFMC) testing system combining europium chelated microparticle (EuCM) with self-driven microfluidic chip assay has been established to unambiguously determine soman (GD) and VX exposure wit...
Source: Analytical Biochemistry - November 15, 2023 Category: Biochemistry Authors: Qiao Lv Pei-Yu Jiang Jun-Mei Xia Long-Hui Liang Bo Chen Yang Yang Long Yan Hui-Lan Yu Chang-Cai Liu Source Type: research
Quantitative profiling of supersulfides naturally occurring in dietary meats and beans
This study investigated the supersulfide profiles of protein-rich foods, including edible animal meat and beans. Quantification of the supersulfide content revealed that natto, chicken liver, and bean sprouts contained abundant supersulfides. In general, the supersulfide content in beans and their derivatives was higher than that in animal meat. The highest proportion (2.15 %) was detected in natto, a traditional Japanese fermented soybean dish. These results suggest that the abundance of supersulfides, especially in foods like natto and bean sprouts, may contribute to their health-promoting properties. Our findings may ha...
Source: Analytical Biochemistry - November 15, 2023 Category: Biochemistry Authors: Shingo Kasamatsu Ayaka Kinno Chiharu Miura Jun-Ichi Hishiyama Kensuke Fukui Shoji Kure Kazunobu Tsumura Tomoaki Ida Tetsuro Matsunaga Takaaki Akaike Hideshi Ihara Source Type: research
Discriminative detection of soman or VX exposure using europium chelated microparticle-based immunofluorescence microfluidic chip
Anal Biochem. 2023 Nov 13:115388. doi: 10.1016/j.ab.2023.115388. Online ahead of print.ABSTRACTThe retrospective detection of organophosphorus nerve agents (OPNAs) exposure has been achieved by the off-site analysis of OPNA-human serum albumin (HSA) adducts using mass spectrometry-based detection approaches. However, few specific methods are accessible for on-site detection. To address this, a novel immunofluorescence microfluidic chip (IFMC) testing system combining europium chelated microparticle (EuCM) with self-driven microfluidic chip assay has been established to unambiguously determine soman (GD) and VX exposure wit...
Source: Analytical Biochemistry - November 15, 2023 Category: Biochemistry Authors: Qiao Lv Pei-Yu Jiang Jun-Mei Xia Long-Hui Liang Bo Chen Yang Yang Long Yan Hui-Lan Yu Chang-Cai Liu Source Type: research
