Comparing in vitro affinity measurements of antibodies to TfR1: Surface plasmon resonance versus on-cell affinity

In conclusion, it is important to evaluate assay parameters including assay orientation, surface capture method, and antibody-format when comparing binding kinetics for TfR1 antibodies. Although it seems possible to determine relative affinities of TfR1 antibodies using the methods described here, both the FLAG-tag TfR1 capture setup and cell assays likely yield apparent affinities that are most translatable in vivo.PMID:38006952 | DOI:10.1016/j.ab.2023.115406

Source: Analytical Biochemistry - November 25, 2023 Category: Biochemistry Authors: Gillian Bonvicini Sunitha Singh Patrik Nygren Mengfei Xiong Stina Syv änen Dag Sehlin Ronny Falk Ken G Andersson Source Type: research