Echinacoside regulates PI3K/AKT/HIF-1 α/VEGF cross signaling axis in proliferation and apoptosis of breast cancer
CONCLUSION: In summary, ECH played an important role in anti-BC by regulating PI3K/AKT/HIF-1α/VEGF signaling pathway. Furthermore, ECH had multi-target and multi-pathway effects, which may be a promising natural compound for treating BC.PMID:37865269 | DOI:10.1016/j.ab.2023.115360 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 21, 2023 Category: Biochemistry Authors: Hongyi Liang Guoliang Yin Guangxi Shi Zhiyong Liu Xiaofei Liu Jingwei Li Source Type: research
Advancement of microfluidic modulation spectroscopy as a highly sensitive protein characterization technique for the detection of small structural changes
This study evaluates and compares the different well plate formats and scan modes of two MMS instruments. The newer Apollo system features a high throughput 96-well plate format and sweep scan mode that allows a 50% reduction in sample volume consumption and measurement time compared to the previous system. By measuring two proteins with drastically different secondary structures, the results demonstrated that the measurements were highly repeatable (>99% repeatability by area of overlap) regardless of the well plate formats or the scan modes. The limit of quantitation (LOQ) for determining structural impurity using the...
Source: Analytical Biochemistry - October 20, 2023 Category: Biochemistry Authors: Tianhui Maria Ma Richard H Huang Valerie I Collins Mats Wikstr öm Dipanwita Batabyal Source Type: research
Nanomaterials for fluorescent detection of vitamin B < sub > 2 < /sub > : A review
Anal Biochem. 2023 Oct 17:115351. doi: 10.1016/j.ab.2023.115351. Online ahead of print.ABSTRACTVitamin B2 plays vital roles in maintaining human health. It is of tremendous significance to construct sensitive sensors of VB2. In this review, we first briefly presented the sensing mechanisms of fluorescent nanomaterials for sensing VB2. Subsequently, the advances of nanomaterials for fluorescent determination of VB2 were highlighted. And sensing performance of traditional approaches and fluorescent nanosensors was further compared. In last section, the challenges and perspectives concerning the topic were discussed.PMID:3785...
Source: Analytical Biochemistry - October 20, 2023 Category: Biochemistry Authors: Yanjuan Luo Yongming Guo Source Type: research
Advancement of microfluidic modulation spectroscopy as a highly sensitive protein characterization technique for the detection of small structural changes
This study evaluates and compares the different well plate formats and scan modes of two MMS instruments. The newer Apollo system features a high throughput 96-well plate format and sweep scan mode that allows a 50% reduction in sample volume consumption and measurement time compared to the previous system. By measuring two proteins with drastically different secondary structures, the results demonstrated that the measurements were highly repeatable (>99% repeatability by area of overlap) regardless of the well plate formats or the scan modes. The limit of quantitation (LOQ) for determining structural impurity using the...
Source: Analytical Biochemistry - October 20, 2023 Category: Biochemistry Authors: Tianhui Maria Ma Richard H Huang Valerie I Collins Mats Wikstr öm Dipanwita Batabyal Source Type: research
Nanomaterials for fluorescent detection of vitamin B < sub > 2 < /sub > : A review
Anal Biochem. 2023 Oct 17:115351. doi: 10.1016/j.ab.2023.115351. Online ahead of print.ABSTRACTVitamin B2 plays vital roles in maintaining human health. It is of tremendous significance to construct sensitive sensors of VB2. In this review, we first briefly presented the sensing mechanisms of fluorescent nanomaterials for sensing VB2. Subsequently, the advances of nanomaterials for fluorescent determination of VB2 were highlighted. And sensing performance of traditional approaches and fluorescent nanosensors was further compared. In last section, the challenges and perspectives concerning the topic were discussed.PMID:3785...
Source: Analytical Biochemistry - October 20, 2023 Category: Biochemistry Authors: Yanjuan Luo Yongming Guo Source Type: research
An ionic liquid-assisted sample preparation method for sensitive integral-membrane proteome analysis
In this study, we present a combined i-soln-assisted proteomic sample preparation platform (termed pTRUST), which is compatible with starting materials in the sub-microgram range, using our previously reported i-soln-based sample preparation strategy (iBOPs) and an in-StageTip technique. This novel and straightforward approach allows for the rapid solubilization and processing of a variety of IMPs from human samples to support highly sensitive mass spectrometry analysis. We also demonstrated that the performance of this technology surpasses that of conventional methods such as filter-aided sample preparation methods, FASP ...
Source: Analytical Biochemistry - October 18, 2023 Category: Biochemistry Authors: Masato Taoka Kota Kamei Akiko Kashima Yuko Nobe Takahiro Takekiyo Takamasa Uekita Tohru Ichimura Source Type: research
An ionic liquid-assisted sample preparation method for sensitive integral-membrane proteome analysis
In this study, we present a combined i-soln-assisted proteomic sample preparation platform (termed pTRUST), which is compatible with starting materials in the sub-microgram range, using our previously reported i-soln-based sample preparation strategy (iBOPs) and an in-StageTip technique. This novel and straightforward approach allows for the rapid solubilization and processing of a variety of IMPs from human samples to support highly sensitive mass spectrometry analysis. We also demonstrated that the performance of this technology surpasses that of conventional methods such as filter-aided sample preparation methods, FASP ...
Source: Analytical Biochemistry - October 18, 2023 Category: Biochemistry Authors: Masato Taoka Kota Kamei Akiko Kashima Yuko Nobe Takahiro Takekiyo Takamasa Uekita Tohru Ichimura Source Type: research
A novel molecularly imprinted electrochemical sensor for the ultrasensitive detection of tert-butylhydroquinone in edible oils
Anal Biochem. 2023 Oct 13;682:115348. doi: 10.1016/j.ab.2023.115348. Online ahead of print.ABSTRACTTert-butylhydroquinone (TBHQ) is widely used to increase the stability of food products; however, it is considered to be a highly unsafe preservative ingredient that has caused serious damage to human health. Thus, in this paper, a novel molecularly imprinted electrochemical sensor was designed for ultrasensitive, and selective detection of TBHQ in edible oils. The sensor was based on the molecularly imprinted polymer (MIP) synthesized with multiwalled carbon nanotube (MWCNT), and gold nanoparticle (GNP), as the coating mater...
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Safaa Hash ım Mohammed Albayatı Zafer Üstündağ Pervin Soylu Source Type: research
Two fluorimetric determinations of acid α-glucosidase activity in dried-blood spot: Pompe disease in Iranian population
CONCLUSIONS: Establishing and implementing these 2 methods for the Iranian population effectively differentiated between healthy and patient individuals. Method II, with its shorter incubation time, demonstrated practicality in the clinical setting.PMID:37821037 | DOI:10.1016/j.ab.2023.115346 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Azadeh Tajmir-Riahi Shohreh Khatami Farzaneh Shemirani Roghieh Mirzazadeh Source Type: research
Method to generate Holliday junction recombination intermediates via RecA-mediated four-strand exchange
Anal Biochem. 2023 Oct 14;682:115347. doi: 10.1016/j.ab.2023.115347. Online ahead of print.ABSTRACTDNA molecules that contain single Holliday junctions have served as model substrates to investigate the pathway in which homologous recombination intermediates are processed. However, the preparation of DNA containing Holliday junctions in high yield remains a challenge. In this work, we used a nicking endonuclease to generate gapped DNA, from which α-structured DNA or figure-8 DNA were created via RecA-mediated reactions. The resulting DNA molecules were found to serve as good substrates for Holliday junction resolvases. Th...
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Han Ngoc Ho Stephen C West Source Type: research
A novel molecularly imprinted electrochemical sensor for the ultrasensitive detection of tert-butylhydroquinone in edible oils
Anal Biochem. 2023 Oct 13;682:115348. doi: 10.1016/j.ab.2023.115348. Online ahead of print.ABSTRACTTert-butylhydroquinone (TBHQ) is widely used to increase the stability of food products; however, it is considered to be a highly unsafe preservative ingredient that has caused serious damage to human health. Thus, in this paper, a novel molecularly imprinted electrochemical sensor was designed for ultrasensitive, and selective detection of TBHQ in edible oils. The sensor was based on the molecularly imprinted polymer (MIP) synthesized with multiwalled carbon nanotube (MWCNT), and gold nanoparticle (GNP), as the coating mater...
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Safaa Hash ım Mohammed Albayatı Zafer Üstündağ Pervin Soylu Source Type: research
Two fluorimetric determinations of acid α-glucosidase activity in dried-blood spot: Pompe disease in Iranian population
CONCLUSIONS: Establishing and implementing these 2 methods for the Iranian population effectively differentiated between healthy and patient individuals. Method II, with its shorter incubation time, demonstrated practicality in the clinical setting.PMID:37821037 | DOI:10.1016/j.ab.2023.115346 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Azadeh Tajmir-Riahi Shohreh Khatami Farzaneh Shemirani Roghieh Mirzazadeh Source Type: research
Method to generate Holliday junction recombination intermediates via RecA-mediated four-strand exchange
Anal Biochem. 2023 Oct 14;682:115347. doi: 10.1016/j.ab.2023.115347. Online ahead of print.ABSTRACTDNA molecules that contain single Holliday junctions have served as model substrates to investigate the pathway in which homologous recombination intermediates are processed. However, the preparation of DNA containing Holliday junctions in high yield remains a challenge. In this work, we used a nicking endonuclease to generate gapped DNA, from which α-structured DNA or figure-8 DNA were created via RecA-mediated reactions. The resulting DNA molecules were found to serve as good substrates for Holliday junction resolvases. Th...
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Han Ngoc Ho Stephen C West Source Type: research
A novel molecularly imprinted electrochemical sensor for the ultrasensitive detection of tert-butylhydroquinone in edible oils
Anal Biochem. 2023 Oct 13;682:115348. doi: 10.1016/j.ab.2023.115348. Online ahead of print.ABSTRACTTert-butylhydroquinone (TBHQ) is widely used to increase the stability of food products; however, it is considered to be a highly unsafe preservative ingredient that has caused serious damage to human health. Thus, in this paper, a novel molecularly imprinted electrochemical sensor was designed for ultrasensitive, and selective detection of TBHQ in edible oils. The sensor was based on the molecularly imprinted polymer (MIP) synthesized with multiwalled carbon nanotube (MWCNT), and gold nanoparticle (GNP), as the coating mater...
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Safaa Hash ım Mohammed Albayatı Zafer Üstündağ Pervin Soylu Source Type: research
Two fluorimetric determinations of acid α-glucosidase activity in dried-blood spot: Pompe disease in Iranian population
CONCLUSIONS: Establishing and implementing these 2 methods for the Iranian population effectively differentiated between healthy and patient individuals. Method II, with its shorter incubation time, demonstrated practicality in the clinical setting.PMID:37821037 | DOI:10.1016/j.ab.2023.115346 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 11, 2023 Category: Biochemistry Authors: Azadeh Tajmir-Riahi Shohreh Khatami Farzaneh Shemirani Roghieh Mirzazadeh Source Type: research
