Detection of DNA using gold nanoparticle-coated silica nanoparticles
We report a sensitive lateral flow assay (LFA) in which the assay colour change originated from reporter labels constructed from silica spheres (radius = 450 nm) coated with approximately 3.9 × 103 gold nanoparticles (radius = 8.5 nm). These reporter labels were modified with DNA and deposited in the conjugation area of an LFA device assembled on wax-patterned Fusion 5 paper. Test and control zones of the device were pre-loaded with capture probe formed by avidin-coated mesoporous silica nanoparticles attached with biotin-tagged DNA sequences. Proof-of-concept was demonstrated by the detection of a partial sequence of the...
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Amir Osman Abdelrazig Patsamon Rijiravanich Sawita Suwannarat Werasak Surareungchai Mithran Somasundrum Source Type: research
Core-shell ZnO@CoO nitrogen doped nano-composites as highly sensitive electrochemical sensor for organophosphate pesticides detection
Anal Biochem. 2023 Dec 7:115422. doi: 10.1016/j.ab.2023.115422. Online ahead of print.ABSTRACTCore-shell ZIF-8@ZIF-67 was synthesized by growing a cobalt-based ZIF-67 on a ZIF-8 seed particle. Herein, through selective etching of the ZIF-8@ZIF-67 core and subsequent direct carbonization, core-shell hollow ZnO@CoO nitrogen-doped nanoporous carbon (HZnO@CoO-NPC) nanocomposites were prepared. HZnO@CoO-NPCs possessed a high nitrogen content, large surface area, high degree of graphitization and excellent electrical conductivity, all of which were attributed to successfully integrating the unique advantages of ZIF-8 and ZIF-67....
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Zhimin Li Xiong Lu Guiqiao Liu Libin Yang Faming Gao Source Type: research
Detection of DNA using gold nanoparticle-coated silica nanoparticles
We report a sensitive lateral flow assay (LFA) in which the assay colour change originated from reporter labels constructed from silica spheres (radius = 450 nm) coated with approximately 3.9 × 103 gold nanoparticles (radius = 8.5 nm). These reporter labels were modified with DNA and deposited in the conjugation area of an LFA device assembled on wax-patterned Fusion 5 paper. Test and control zones of the device were pre-loaded with capture probe formed by avidin-coated mesoporous silica nanoparticles attached with biotin-tagged DNA sequences. Proof-of-concept was demonstrated by the detection of a partial sequence of the...
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Amir Osman Abdelrazig Patsamon Rijiravanich Sawita Suwannarat Werasak Surareungchai Mithran Somasundrum Source Type: research
Core-shell ZnO@CoO nitrogen doped nano-composites as highly sensitive electrochemical sensor for organophosphate pesticides detection
Anal Biochem. 2023 Dec 7:115422. doi: 10.1016/j.ab.2023.115422. Online ahead of print.ABSTRACTCore-shell ZIF-8@ZIF-67 was synthesized by growing a cobalt-based ZIF-67 on a ZIF-8 seed particle. Herein, through selective etching of the ZIF-8@ZIF-67 core and subsequent direct carbonization, core-shell hollow ZnO@CoO nitrogen-doped nanoporous carbon (HZnO@CoO-NPC) nanocomposites were prepared. HZnO@CoO-NPCs possessed a high nitrogen content, large surface area, high degree of graphitization and excellent electrical conductivity, all of which were attributed to successfully integrating the unique advantages of ZIF-8 and ZIF-67....
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Zhimin Li Xiong Lu Guiqiao Liu Libin Yang Faming Gao Source Type: research
Detection of DNA using gold nanoparticle-coated silica nanoparticles
We report a sensitive lateral flow assay (LFA) in which the assay colour change originated from reporter labels constructed from silica spheres (radius = 450 nm) coated with approximately 3.9 × 103 gold nanoparticles (radius = 8.5 nm). These reporter labels were modified with DNA and deposited in the conjugation area of an LFA device assembled on wax-patterned Fusion 5 paper. Test and control zones of the device were pre-loaded with capture probe formed by avidin-coated mesoporous silica nanoparticles attached with biotin-tagged DNA sequences. Proof-of-concept was demonstrated by the detection of a partial sequence of the...
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Amir Osman Abdelrazig Patsamon Rijiravanish Sawita Suwannarat Werasak Surareungchai Mithran Somasundrum Source Type: research
Core-shell ZnO@CoO nitrogen doped nano-composites as highly sensitive electrochemical sensor for organophosphate pesticides detection
Anal Biochem. 2023 Dec 7:115422. doi: 10.1016/j.ab.2023.115422. Online ahead of print.ABSTRACTCore-shell ZIF-8@ZIF-67 was synthesized by growing a cobalt-based ZIF-67 on a ZIF-8 seed particle. Herein, through selective etching of the ZIF-8@ZIF-67 core and subsequent direct carbonization, core-shell hollow ZnO@CoO nitrogen-doped nanoporous carbon (HZnO@CoO-NPC) nanocomposites were prepared. HZnO@CoO-NPCs possessed a high nitrogen content, large surface area, high degree of graphitization and excellent electrical conductivity, all of which were attributed to successfully integrating the unique advantages of ZIF-8 and ZIF-67....
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Zhimin Li Xiong Lu Guiqiao Liu Libin Yang Faming Gao Source Type: research
Detection of DNA using gold nanoparticle-coated silica nanoparticles
We report a sensitive lateral flow assay (LFA) in which the assay colour change originated from reporter labels constructed from silica spheres (radius = 450 nm) coated with approximately 3.9 × 103 gold nanoparticles (radius = 8.5 nm). These reporter labels were modified with DNA and deposited in the conjugation area of an LFA device assembled on wax-patterned Fusion 5 paper. Test and control zones of the device were pre-loaded with capture probe formed by avidin-coated mesoporous silica nanoparticles attached with biotin-tagged DNA sequences. Proof-of-concept was demonstrated by the detection of a partial sequence of the...
Source: Analytical Biochemistry - December 9, 2023 Category: Biochemistry Authors: Amir Osman Abdelrazig Patsamon Rijiravanish Sawita Suwannarat Werasak Surareungchai Mithran Somasundrum Source Type: research
Separation of full and empty adeno-associated virus capsids by anion-exchange chromatography using choline-type salts
Anal Biochem. 2023 Dec 5:115421. doi: 10.1016/j.ab.2023.115421. Online ahead of print.ABSTRACTDevelopment of clinically desirable adeno-associated virus (AAV) vectors with optimal genome design requires rapid and accurate analytical methods to assess AAV quality. Anion-exchange (AEX) chromatography provides a powerful analytical method for full/empty AAV capsid ratio determination. However, the current AEX methodology for separation of empty and full AAV capsids largely relies on the use of the highly toxic tetramethylammonium chloride (TMAC). Here, we describe a novel analytical AEX method for separation of empty and full...
Source: Analytical Biochemistry - December 7, 2023 Category: Biochemistry Authors: Sam Kurth Tianyu Li Alana Hausker William E Evans Romain Dabre Egbert M üller Jukka Kervinen Source Type: research
Separation of full and empty adeno-associated virus capsids by anion-exchange chromatography using choline-type salts
Anal Biochem. 2023 Dec 5:115421. doi: 10.1016/j.ab.2023.115421. Online ahead of print.ABSTRACTDevelopment of clinically desirable adeno-associated virus (AAV) vectors with optimal genome design requires rapid and accurate analytical methods to assess AAV quality. Anion-exchange (AEX) chromatography provides a powerful analytical method for full/empty AAV capsid ratio determination. However, the current AEX methodology for separation of empty and full AAV capsids largely relies on the use of the highly toxic tetramethylammonium chloride (TMAC). Here, we describe a novel analytical AEX method for separation of empty and full...
Source: Analytical Biochemistry - December 7, 2023 Category: Biochemistry Authors: Sam Kurth Tianyu Li Alana Hausker William E Evans Romain Dabre Egbert M üller Jukka Kervinen Source Type: research
Separation of full and empty adeno-associated virus capsids by anion-exchange chromatography using choline-type salts
Anal Biochem. 2023 Dec 5:115421. doi: 10.1016/j.ab.2023.115421. Online ahead of print.ABSTRACTDevelopment of clinically desirable adeno-associated virus (AAV) vectors with optimal genome design requires rapid and accurate analytical methods to assess AAV quality. Anion-exchange (AEX) chromatography provides a powerful analytical method for full/empty AAV capsid ratio determination. However, the current AEX methodology for separation of empty and full AAV capsids largely relies on the use of the highly toxic tetramethylammonium chloride (TMAC). Here, we describe a novel analytical AEX method for separation of empty and full...
Source: Analytical Biochemistry - December 7, 2023 Category: Biochemistry Authors: Sam Kurth Tianyu Li Alana Hausker William E Evans Romain Dabre Egbert M üller Jukka Kervinen Source Type: research
An insight into the state of nanotechnology-based electrochemical biosensors for PCOS detection: Mini review
Anal Biochem. 2023 Nov 29:115412. doi: 10.1016/j.ab.2023.115412. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting many women of reproductive age all over the world. PCOS is associated with the onset of enduring health complications, notably diabetes and cardiovascular diseases. Furthermore, PCOS escalates the propensity for conditions such as obesity, insulin resistance, and dyslipidemia, which can potentially culminate in life-threatening scenarios. A pervasive predicament surrounding PCOS pertains to its underdiagnosis due to discrepancies in diagnosti...
Source: Analytical Biochemistry - December 1, 2023 Category: Biochemistry Authors: Nidhi Chauhan Sakshi Pareek Warren Rosario Rachna Rawal Utkarsh Jain Source Type: research
Development of a reporter gene-based assay for the bioactivity determination of rhLH pharmaceutical products
Anal Biochem. 2023 Nov 29;686:115413. doi: 10.1016/j.ab.2023.115413. Online ahead of print.ABSTRACTTo establish an in vitro biological activity detection method for luteinizing hormone (LH), the hLHCGR-CREB-HEK293 cell line was constructed to stably express human luteinizing hormone/chorionic gonadotropin receptor (hLHCGR). After optimization, the rhLH starting working concentration was 800 mIU/mL with 4-fold serial dilutions, 10 concentrations and an incubation time of 5 h. The method was confirmed to be highly specific, with good accuracy, precision and linearity, meeting the needs of process research and release testing...
Source: Analytical Biochemistry - December 1, 2023 Category: Biochemistry Authors: Defang Tang Xiufang Song Yanjiao Du Jiaxin Wang Yuqing Lei Bin Chen Source Type: research
An insight into the state of nanotechnology-based electrochemical biosensors for PCOS detection: Mini review
Anal Biochem. 2023 Nov 29:115412. doi: 10.1016/j.ab.2023.115412. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting many women of reproductive age all over the world. PCOS is associated with the onset of enduring health complications, notably diabetes and cardiovascular diseases. Furthermore, PCOS escalates the propensity for conditions such as obesity, insulin resistance, and dyslipidemia, which can potentially culminate in life-threatening scenarios. A pervasive predicament surrounding PCOS pertains to its underdiagnosis due to discrepancies in diagnosti...
Source: Analytical Biochemistry - December 1, 2023 Category: Biochemistry Authors: Nidhi Chauhan Sakshi Pareek Warren Rosario Rachna Rawal Utkarsh Jain Source Type: research
Development of a reporter gene-based assay for the bioactivity determination of rhLH pharmaceutical products
Anal Biochem. 2023 Nov 29;686:115413. doi: 10.1016/j.ab.2023.115413. Online ahead of print.ABSTRACTTo establish an in vitro biological activity detection method for luteinizing hormone (LH), the hLHCGR-CREB-HEK293 cell line was constructed to stably express human luteinizing hormone/chorionic gonadotropin receptor (hLHCGR). After optimization, the rhLH starting working concentration was 800 mIU/mL with 4-fold serial dilutions, 10 concentrations and an incubation time of 5 h. The method was confirmed to be highly specific, with good accuracy, precision and linearity, meeting the needs of process research and release testing...
Source: Analytical Biochemistry - December 1, 2023 Category: Biochemistry Authors: Defang Tang Xiufang Song Yanjiao Du Jiaxin Wang Yuqing Lei Bin Chen Source Type: research
An insight into the state of nanotechnology-based electrochemical biosensors for PCOS detection: Mini review
Anal Biochem. 2023 Nov 29:115412. doi: 10.1016/j.ab.2023.115412. Online ahead of print.ABSTRACTPolycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting many women of reproductive age all over the world. PCOS is associated with the onset of enduring health complications, notably diabetes and cardiovascular diseases. Furthermore, PCOS escalates the propensity for conditions such as obesity, insulin resistance, and dyslipidemia, which can potentially culminate in life-threatening scenarios. A pervasive predicament surrounding PCOS pertains to its underdiagnosis due to discrepancies in diagnosti...
Source: Analytical Biochemistry - December 1, 2023 Category: Biochemistry Authors: Nidhi Chauhan Sakshi Pareek Warren Rosario Rachna Rawal Utkarsh Jain Source Type: research
