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A comprehensive guide to western blotting for tendon research
The objective of this article is to provide a detailed protocol, elucidating each step, and making it easily replicable for researchers. The study focused on the Achilles tendons of Sprague-Dawley rats, emphasizing the need for a tailored approach in working with this tissue. By addressing the nuances of protein extraction from the dense and fibrous tendons, our protocol aims to facilitate the reproducibility of Western blotting experiments, contributing to a better understanding of this tissue.PMID:38570024 | DOI:10.1016/j.ab.2024.115528 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - April 3, 2024 Category: Biochemistry Authors: Ana Paula Cremasco Takano Diego Pulzatto Cury Source Type: research

Identification of volatile biomarkers for lung cancer from different histological sources: A comprehensive study
Anal Biochem. 2024 Mar 31:115527. doi: 10.1016/j.ab.2024.115527. Online ahead of print.ABSTRACTThe identification of noninvasive volatile biomarkers for lung cancer is a significant clinical challenge. Through in vitro studies, the recognition of altered metabolism in cell volatile organic compound (VOC) emitting profile, along with the occurrence of oncogenesis, provides insight into the biochemical pathways involved in the production and metabolism of lung cancer volatile biomarkers. In this research, for the first time, a comprehensive comparative analysis of the volatile metabolites in NSCLS cells (A549), SCLC cells (H...
Source: Analytical Biochemistry - April 2, 2024 Category: Biochemistry Authors: Wei Lv Wenmin Shi Zhijuan Zhang Lihua Ru Weisheng Feng Hanxiao Tang Xiangqi Wang Source Type: research

Development of a back-titration assay to quantitate functional lympho-epithelial Kazal-type inhibitors (LEKTI) in skin samples
Anal Biochem. 2024 Mar 29:115524. doi: 10.1016/j.ab.2024.115524. Online ahead of print.ABSTRACTThe lympho-epithelial Kazal-type inhibitors (LEKTI) are key to control skin turnover, and their absence causes Netherton syndrome. For clinical sample testing of LEKTI-based therapies, a robust analytical method to measure LEKTI-like activity in skin is required. This work reports on the development of a back-titration method to determine incremental LEKTI-like activity in skin samples. The method meets the analytical requirements for study sample testing, and reliable quantification can be achieved with negligible skin matrix in...
Source: Analytical Biochemistry - March 31, 2024 Category: Biochemistry Authors: Dorival Martins Marie-Aude Pinoteau Roger Leger Source Type: research

Lantern-type G-quadruplex fluorescent sensors for detecting divalent metal ions
Anal Biochem. 2024 Mar 28:115525. doi: 10.1016/j.ab.2024.115525. Online ahead of print.ABSTRACTThree thioflavin T (ThT) derivatives, namely ThT/ethylenediaminetetraacetic acid conjugates (E1T, E2T, and E1T1P), were designed and synthesized as sensing components for divalent metal ion detection. Furthermore, these ThT derivatives were used to design lantern-type G-quadruplex (G4) fluorescent sensors. The fluorescence intensities of the ThT derivatives decreased by 1.2- to 5.6-folds in the presence of Ni2+ and Cu2+, respectively, regardless of the topology of the utilized G4. Conversely, when Mn2+ and Zn2+ coexisted in antip...
Source: Analytical Biochemistry - March 30, 2024 Category: Biochemistry Authors: Tomoko Wariishi Yuka Kataoka Tomoaki Nakamura Yuuya Kasahara Masataka Kuroda Satoshi Obika Masayasu Kuwahara Source Type: research

HemoDL: Hemolytic peptides prediction by double ensemble engines from Rich sequence-derived and transformer-enhanced information
In this study, we offer the HemoDL ensemble learning model, which learns the distinct distribution of sequence characteristics for predicting the hemolytic activity of peptides using a double LightGBM framework. To determine the most informative encoding features, we compare 17 widely used features across four benchmark datasets. Our investigation reveals that CTD, BPF, Charge, AAC, GDPC, ATC, QSO, and transformer-based features exhibit more positive contributions to detecting the hemolytic activity of peptides. Comparison with eight state-of-the-art methods demonstrates that HemoDL outperforms other models, attaining high...
Source: Analytical Biochemistry - March 29, 2024 Category: Biochemistry Authors: Sen Yang Piao Xu Source Type: research

Exploring the potential of SrTi 0.7 Fe 0.3 O 3 perovskite/chitosan nanosheets for the development of a label-free electrochemical sensing assay for determination of naproxen in human plasma samples
Anal Biochem. 2024 Mar 24:115513. doi: 10.1016/j.ab.2024.115513. Online ahead of print.ABSTRACTNaproxen is a nonsteroidal anti-inflammatory drug used to treat nonrheumatic inflammation, migraine, and gout. Therefore, the determination of naproxen in pharmaceutical and biological samples is of particular importance. In the present work, SrTi0.7Fe0.3O3 perovskite/Chitosan nanosheets were used to modify the surface of a glassy carbon electrode (GCE) for highly sensitive determination of naproxen. To ensure the successful synthesis of the perovskite nanosheets, morphological studies including scanning electron microscopy (SEM)...
Source: Analytical Biochemistry - March 26, 2024 Category: Biochemistry Authors: Roghayeh Mahmoudi-Maleki Mir Reza Majidi Hessamaddin Sohrabi Elham Mahmoudi Homa Fooladvand Ali Coruh Aligholi Niaei Source Type: research

Self-signal electrochemical identification of circulating tumor DNA employing poly-xanthurenic acid assembled on black phosphorus nanosheets
Anal Biochem. 2024 Mar 25;690:115512. doi: 10.1016/j.ab.2024.115512. Online ahead of print.ABSTRACTA self-signal electrochemical identification interface was prepared for the determination of circulating tumor DNA (ctDNA) in peripheral blood based on poly-xanthurenic acid (PXTA) assembled on black phosphorus nanosheets (BPNSs) acquired through simple ultrasonication method. The BPNSs with large surface area could be integrated with the xanthurenic acid (XTA) monomers by right of physisorption, and hence improved the electropolymerization efficiency and was beneficial to the enlargement of the signal response of PXTA. The a...
Source: Analytical Biochemistry - March 25, 2024 Category: Biochemistry Authors: Xinyu Yan Wei Zhang Jimin Yang Source Type: research

Polysaccharide-coated polystyrene microtiter plates for improved protein immobilization
Anal Biochem. 2024 Mar 22:115514. doi: 10.1016/j.ab.2024.115514. Online ahead of print.NO ABSTRACTPMID:38522810 | DOI:10.1016/j.ab.2024.115514 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Anton Plekhanov Sergey Shishlyannikov Source Type: research

Simultaneous determination of plasma protein binding of five C-glycosylflavones from TFDS by rapid equilibrium dialysis
In this study, the plasma protein binding of these compounds was determined for the first time in rat and human plasma by rapid equilibrium dialysis combined with HPLC-MS/MS method. The developed method was validated in terms of specificity, linearity, accuracy, precision, extraction effect, matrix effect, and stability. Schaftoside, vicenin-1, vicenin-2, and vicenin-3 exhibited moderate plasma protein binding, ranging from 56.6% to 61.5% in rat plasma and 55.0%-62.9% in human plasma. In comparison, isovitexin demonstrated a higher plasma protein binding in the range of 92.3-93.1% and 95.1-96.2% in rat and human plasma, re...
Source: Analytical Biochemistry - March 24, 2024 Category: Biochemistry Authors: Nan Ding Chen Chao Yishu Liu Peiyong Zheng Xue Li Ming Yang Source Type: research