Direct competitive assay for HER2 detection in human plasma using Bloch surface wave-based biosensors
Anal Biochem. 2023 Oct 30:115374. doi: 10.1016/j.ab.2023.115374. Online ahead of print.ABSTRACTThe overexpression and/or amplification of the HER2/neu oncogene has been proposed as a prognostic marker in breast cancer. The detection of the related peptide HER2 remains a grand challenge in cancer diagnosis and for therapeutic decision-making. Here, we used a biosensing device based on Bloch Surface Waves excited on a one-dimensional photonic crystal (1DPC) as valid alternative to standard techniques. The 1DPC was optimized to operate in the visible spectrum and the biosensor optics has been designed to combine label-free an...
Source: Analytical Biochemistry - November 1, 2023 Category: Biochemistry Authors: Tommaso Pileri Alberto Sinibaldi Agostino Occhicone Norbert Danz Elena Giordani Matteo Allegretti Frank Sonntag Peter Munzert Patrizio Giacomini Francesco Michelotti Source Type: research
Preclinical pharmacokinetic and in vitro metabolic stability study of lysosomotropic autophagy inhibitor, IITZ-01 in mice by using UPLC-MS/MS
Anal Biochem. 2023 Oct 29:115333. doi: 10.1016/j.ab.2023.115333. Online ahead of print.ABSTRACTThe present study evaluates the pharmacokinetics and metabolic stability of a novel lysosomotropic autophagy inhibitor, IITZ-01 using an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS). It is required as this lead molecule awaits pre-clinical studies for development because of significant therapeutic outcomes in triple-negative breast cancer and renal cancer. A bioanalytical method for the quantitative determination of IITZ-01 in the plasma of mice was developed using the UPLC-MS/MS technique. ...
Source: Analytical Biochemistry - October 31, 2023 Category: Biochemistry Authors: Basveshwar Gawali Aishwarya Jala Jagadeesh Kumar Gangasani Eswara Rao Puppala Roshan M Borkar V G M Naidu Source Type: research
Preclinical pharmacokinetic and in vitro metabolic stability study of lysosomotropic autophagy inhibitor, IITZ-01 in mice by using UPLC-MS/MS
Anal Biochem. 2023 Oct 29:115333. doi: 10.1016/j.ab.2023.115333. Online ahead of print.ABSTRACTThe present study evaluates the pharmacokinetics and metabolic stability of a novel lysosomotropic autophagy inhibitor, IITZ-01 using an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS). It is required as this lead molecule awaits pre-clinical studies for development because of significant therapeutic outcomes in triple-negative breast cancer and renal cancer. A bioanalytical method for the quantitative determination of IITZ-01 in the plasma of mice was developed using the UPLC-MS/MS technique. ...
Source: Analytical Biochemistry - October 31, 2023 Category: Biochemistry Authors: Basveshwar Gawali Aishwarya Jala Jagadeesh Kumar Gangasani Eswara Rao Puppala Roshan M Borkar V G M Naidu Source Type: research
Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection
Anal Biochem. 2023 Oct 25:115370. doi: 10.1016/j.ab.2023.115370. Online ahead of print.ABSTRACTAs the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Jiawang Xiang Bing Zhang Yani Shi Yanfei Wen Yuan Yuan Jianying Lin Zhihuan Zhao Jing Li Yan Cheng Source Type: research
A review on current diagnostic tools and potential optical absorption spectroscopy for HFMD detection
Anal Biochem. 2023 Oct 25:115368. doi: 10.1016/j.ab.2023.115368. Online ahead of print.ABSTRACTHand, Foot, and Mouth Disease (HFMD) is an outbreak infectious disease that can easily spread among children under the age of five. The most common causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16), but infection caused by EV71 is more associated with fatalities due to severe neurological disorders. The present diagnosis methods rely on physical examinations by the doctors and further confirmation by laboratories detection methods such as viral culture and polymerase chain reaction. Clinical signs ...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Fatin Hamimi Mustafa Ahmad Aiman Zuhaily Ahmad Munawar Basmah Abdul Basir Rafidah Hanim Shueb Ahmad Adebayo Irekeola Wan Zakiah Wan Ismail Juliza Jamaludin Sharma Rao Al Balakrishnan Mus'ab Sahrim Nik Yusnoraini Yusof Irneza Ismail Source Type: research
Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection
Anal Biochem. 2023 Oct 25:115370. doi: 10.1016/j.ab.2023.115370. Online ahead of print.ABSTRACTAs the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Jiawang Xiang Bing Zhang Yani Shi Yanfei Wen Yuan Yuan Jianying Lin Zhihuan Zhao Jing Li Yan Cheng Source Type: research
A review on current diagnostic tools and potential optical absorption spectroscopy for HFMD detection
Anal Biochem. 2023 Oct 25:115368. doi: 10.1016/j.ab.2023.115368. Online ahead of print.ABSTRACTHand, Foot, and Mouth Disease (HFMD) is an outbreak infectious disease that can easily spread among children under the age of five. The most common causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16), but infection caused by EV71 is more associated with fatalities due to severe neurological disorders. The present diagnosis methods rely on physical examinations by the doctors and further confirmation by laboratories detection methods such as viral culture and polymerase chain reaction. Clinical signs ...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Fatin Hamimi Mustafa Ahmad Aiman Zuhaily Ahmad Munawar Basmah Abdul Basir Rafidah Hanim Shueb Ahmad Adebayo Irekeola Wan Zakiah Wan Ismail Juliza Jamaludin Sharma Rao Al Balakrishnan Mus'ab Sahrim Nik Yusnoraini Yusof Irneza Ismail Source Type: research
Isoniazide modified Ag nanoparticles triggered photothermal immunoassay for carcinoembryonic antigen detection
Anal Biochem. 2023 Oct 25:115370. doi: 10.1016/j.ab.2023.115370. Online ahead of print.ABSTRACTAs the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay. The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Jiawang Xiang Bing Zhang Yani Shi Yanfei Wen Yuan Yuan Jianying Lin Zhihuan Zhao Jing Li Yan Cheng Source Type: research
A review on current diagnostic tools and potential optical absorption spectroscopy for HFMD detection
Anal Biochem. 2023 Oct 25:115368. doi: 10.1016/j.ab.2023.115368. Online ahead of print.ABSTRACTHand, Foot, and Mouth Disease (HFMD) is an outbreak infectious disease that can easily spread among children under the age of five. The most common causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16), but infection caused by EV71 is more associated with fatalities due to severe neurological disorders. The present diagnosis methods rely on physical examinations by the doctors and further confirmation by laboratories detection methods such as viral culture and polymerase chain reaction. Clinical signs ...
Source: Analytical Biochemistry - October 27, 2023 Category: Biochemistry Authors: Fatin Hamimi Mustafa Ahmad Aiman Zuhaily Ahmad Munawar Basmah Abdul Basir Rafidah Hanim Shueb Ahmad Adebayo Irekeola Wan Zakiah Wan Ismail Juliza Jamaludin Sharma Rao Al Balakrishnan Mus'ab Sahrim Nik Yusnoraini Yusof Irneza Ismail Source Type: research
A platform for qualitative and quantitative characterization of α-Gal and NeuGc at the oligosaccharide level
This study introduces a sensitive and reliable analytical approach for the qualitative and quantitative assessment of α-Gal and NeuGc in glycoprotein pharmaceuticals. The methodology offers significant potential for application in process control and optimization of glycoprotein production, aimed at minimizing immunogenicity and enhancing product quality.PMID:37866525 | DOI:10.1016/j.ab.2023.115362 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Weicheng Ren Lan Yang Jia Feng Shuyue Wang Qi Hu Hailong Liu Jinliang Zhang Zhiwei Wang Menghan Yan Hongwei Yu Yingwu Wang Source Type: research
Acid hydrolysis conditions for quantification of meningococcal X polysaccharide in a pentavalent vaccine using HPAEC-PAD/ESI-MS
Anal Biochem. 2023 Oct 20:115363. doi: 10.1016/j.ab.2023.115363. Online ahead of print.ABSTRACTA selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from...
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Saurav Ghosh Rakesh Pawar Vivek Kangralkar Asha D Mallya Rajeev M Dhere Barbara Bolgiano Neil Ravenscroft Source Type: research
A platform for qualitative and quantitative characterization of α-Gal and NeuGc at the oligosaccharide level
This study introduces a sensitive and reliable analytical approach for the qualitative and quantitative assessment of α-Gal and NeuGc in glycoprotein pharmaceuticals. The methodology offers significant potential for application in process control and optimization of glycoprotein production, aimed at minimizing immunogenicity and enhancing product quality.PMID:37866525 | DOI:10.1016/j.ab.2023.115362 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Weicheng Ren Lan Yang Jia Feng Shuyue Wang Qi Hu Hailong Liu Jinliang Zhang Zhiwei Wang Menghan Yan Hongwei Yu Yingwu Wang Source Type: research
Acid hydrolysis conditions for quantification of meningococcal X polysaccharide in a pentavalent vaccine using HPAEC-PAD/ESI-MS
Anal Biochem. 2023 Oct 20:115363. doi: 10.1016/j.ab.2023.115363. Online ahead of print.ABSTRACTA selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from...
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Saurav Ghosh Rakesh Pawar Vivek Kangralkar Asha D Mallya Rajeev M Dhere Barbara Bolgiano Neil Ravenscroft Source Type: research
A platform for qualitative and quantitative characterization of α-Gal and NeuGc at the oligosaccharide level
This study introduces a sensitive and reliable analytical approach for the qualitative and quantitative assessment of α-Gal and NeuGc in glycoprotein pharmaceuticals. The methodology offers significant potential for application in process control and optimization of glycoprotein production, aimed at minimizing immunogenicity and enhancing product quality.PMID:37866525 | DOI:10.1016/j.ab.2023.115362 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Weicheng Ren Lan Yang Jia Feng Shuyue Wang Qi Hu Hailong Liu Jinliang Zhang Zhiwei Wang Menghan Yan Hongwei Yu Yingwu Wang Source Type: research
Acid hydrolysis conditions for quantification of meningococcal X polysaccharide in a pentavalent vaccine using HPAEC-PAD/ESI-MS
Anal Biochem. 2023 Oct 20:115363. doi: 10.1016/j.ab.2023.115363. Online ahead of print.ABSTRACTA selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from...
Source: Analytical Biochemistry - October 22, 2023 Category: Biochemistry Authors: Saurav Ghosh Rakesh Pawar Vivek Kangralkar Asha D Mallya Rajeev M Dhere Barbara Bolgiano Neil Ravenscroft Source Type: research
