Mechanism of Xing 9 Ling tablet candy for alcoholic liver disease based on network pharmacology
In this study, we established a method for the determination of X9LTC content for the first time, and predicted its active ingredient and mechanism of action in treating ALD, providing theoretical basis for further research.PMID:38621605 | DOI:10.1016/j.ab.2024.115534 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - April 15, 2024 Category: Biochemistry Authors: Xiaomei Zhao Qiong Qu Ying Zhang Peiyuan Zhao Xinbo Zhang Yingying Tang Xuan Lei Xuan Wei Xiao Song Source Type: research
Point-of-care testing of methamphetamine and cocaine utilizing wearable sensors
Anal Biochem. 2024 Apr 13:115526. doi: 10.1016/j.ab.2024.115526. Online ahead of print.ABSTRACTThe imperative for the point-of-care testing of methamphetamine and cocaine in drug abuse prevention necessitates innovative solutions. To address this need, we have introduced a multi-channel wearable sensor harnessing CRISPR/Cas12a system. A CRISPR/Cas12a based system, integrated with aptamers specific to methamphetamine and cocaine, has been engineered. These aptamers function as signal-mediated intermediaries, converting methamphetamine and cocaine into nucleic acid signals, subsequently generating single-stranded DNA to acti...
Source: Analytical Biochemistry - April 15, 2024 Category: Biochemistry Authors: Ying Wang Ke Li Weijian Shen Xingxu Huang Lina Wu Source Type: research
Mechanism of Xing 9 Ling tablet candy for alcoholic liver disease based on network pharmacology
In this study, we established a method for the determination of X9LTC content for the first time, and predicted its active ingredient and mechanism of action in treating ALD, providing theoretical basis for further research.PMID:38621605 | DOI:10.1016/j.ab.2024.115534 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - April 15, 2024 Category: Biochemistry Authors: Xiaomei Zhao Qiong Qu Ying Zhang Peiyuan Zhao Xinbo Zhang Yingying Tang Xuan Lei Xuan Wei Xiao Song Source Type: research
Point-of-care testing of methamphetamine and cocaine utilizing wearable sensors
Anal Biochem. 2024 Apr 13:115526. doi: 10.1016/j.ab.2024.115526. Online ahead of print.ABSTRACTThe imperative for the point-of-care testing of methamphetamine and cocaine in drug abuse prevention necessitates innovative solutions. To address this need, we have introduced a multi-channel wearable sensor harnessing CRISPR/Cas12a system. A CRISPR/Cas12a based system, integrated with aptamers specific to methamphetamine and cocaine, has been engineered. These aptamers function as signal-mediated intermediaries, converting methamphetamine and cocaine into nucleic acid signals, subsequently generating single-stranded DNA to acti...
Source: Analytical Biochemistry - April 15, 2024 Category: Biochemistry Authors: Ying Wang Ke Li Weijian Shen Xingxu Huang Lina Wu Source Type: research
Mechanism of Xing 9 Ling tablet candy for alcoholic liver disease based on network pharmacology
In this study, we established a method for the determination of X9LTC content for the first time, and predicted its active ingredient and mechanism of action in treating ALD, providing theoretical basis for further research.PMID:38621605 | DOI:10.1016/j.ab.2024.115534 (Source: Analytical Biochemistry)
Source: Analytical Biochemistry - April 15, 2024 Category: Biochemistry Authors: Xiaomei Zhao Qiong Qu Ying Zhang Peiyuan Zhao Xinbo Zhang Yingying Tang Xuan Lei Xuan Wei Xiao Song Source Type: research
The bisulfite reaction with cytosine and genomic DNA structure
Anal Biochem. 2024 Apr 10:115532. doi: 10.1016/j.ab.2024.115532. Online ahead of print.ABSTRACTThe bisulfite reaction with native DNA has been extensively employed in the detection of non-B DNA structures that can form spontaneously in DNA. These sequences are dynamic in that they can adopt both normal Watson-Crick paired B-DNA or unusual structures like the Triplex, G-Quadruplex, i-motif and Cruciform or Hairpin. Considerable evidence now suggests that these dynamic sequences play roles in both epigenetics and mutagenesis. The bisulfite reaction with native DNA offers a key approach to their detection. In this application...
Source: Analytical Biochemistry - April 12, 2024 Category: Biochemistry Authors: Steven S Smith Source Type: research
The bisulfite reaction with cytosine and genomic DNA structure
Anal Biochem. 2024 Apr 10:115532. doi: 10.1016/j.ab.2024.115532. Online ahead of print.ABSTRACTThe bisulfite reaction with native DNA has been extensively employed in the detection of non-B DNA structures that can form spontaneously in DNA. These sequences are dynamic in that they can adopt both normal Watson-Crick paired B-DNA or unusual structures like the Triplex, G-Quadruplex, i-motif and Cruciform or Hairpin. Considerable evidence now suggests that these dynamic sequences play roles in both epigenetics and mutagenesis. The bisulfite reaction with native DNA offers a key approach to their detection. In this application...
Source: Analytical Biochemistry - April 12, 2024 Category: Biochemistry Authors: Steven S Smith Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4;690:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the asse...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4;690:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the asse...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4;690:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the asse...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4;690:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the asse...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the assessme...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Detection of isoflavones and phytoestrogen-rich plant extracts binding to estrogen receptor β using a yeast-based fluorescent assay
Anal Biochem. 2024 Apr 4:115529. doi: 10.1016/j.ab.2024.115529. Online ahead of print.ABSTRACTAlchemilla vulgaris L., Trifolium pratense L. and Glycyrrhiza glabra L. are important remedies in traditional medicine, known for many usages, including treating gynecological diseases. Despite folkloric use of the plant materials, there is a lack of scientific data to support their therapeutic application. The aims of the present study were to evaluate the relative binding affinities (RBAs) of plant-derived phytoestrogens for estrogen receptor β (ERβ) using fluorescent biosensor in yeast and to apply this assay for the assessme...
Source: Analytical Biochemistry - April 6, 2024 Category: Biochemistry Authors: Sofija Beki ć Edward Petri Sanja Krsti ć Andjelka Ćelić Suzana Jovanovi ć-Šanta Source Type: research
Development and characterization of discontinuous atmospheric pressure interface - Dual pressure chamber miniature mass spectrometer
This study introduces a novel miniature mass spectrometer featuring a discontinuous atmospheric pressure interface and a dual pressure chamber. Compared to conventional single-chamber, discontinuous sampling interface mass spectrometers, the newly developed instrument demonstrates a more than tenfold improvement in detection efficiency. This significant enhancement is achieved without the need for complex control of switch coupling time series, thereby streamlining the circuit design and improving the instrument's fault tolerance. Furthermore, by capitalizing on the benefits of discontinuous sampling, the instrument reduce...
Source: Analytical Biochemistry - April 4, 2024 Category: Biochemistry Authors: Hua Li Qi Jing-Hao Li Ya-Qing Chen Shen-Shu Lu Xin-Qiong Source Type: research
Development and characterization of discontinuous atmospheric pressure interface - Dual pressure chamber miniature mass spectrometer
This study introduces a novel miniature mass spectrometer featuring a discontinuous atmospheric pressure interface and a dual pressure chamber. Compared to conventional single-chamber, discontinuous sampling interface mass spectrometers, the newly developed instrument demonstrates a more than tenfold improvement in detection efficiency. This significant enhancement is achieved without the need for complex control of switch coupling time series, thereby streamlining the circuit design and improving the instrument's fault tolerance. Furthermore, by capitalizing on the benefits of discontinuous sampling, the instrument reduce...
Source: Analytical Biochemistry - April 4, 2024 Category: Biochemistry Authors: Hua Li Qi Jing-Hao Li Ya-Qing Chen Shen-Shu Lu Xin-Qiong Source Type: research
