Protease-activated receptor 2 drives migration in a colon cancer cell line but not in non-cancerous human epithelial cells
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00284.2023. Online ahead of print.ABSTRACTThe inflamed mucosa contains a complex assortment of proteases which may participate in wound healing or in the development of inflammation-associated colon cancer. We sought to determine the role of protease-activated receptor 2 (PAR2) in epithelial wound healing in both untransformed and transformed colonic epithelial cells. Monolayers of primary epithelial cells derived from organoids cultivated from patient colonic biopsies, and of the T84 colon cancer cell line, were grown to confluence, wounded in the pre...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Larissa Lucena P érico Andrew J Vegso Cristiane H Baggio Wallace K MacNaughton Source Type: research
Hepatic LGR4 aggravates cholestasis-induced liver injury in mice
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00127.2023. Online ahead of print.ABSTRACTCurrent therapy for hepatic injury induced by accumulation of bile acid is limited. Leucine-rich repeat G-protein-coupled receptor 4 (LGR4), also known as GPR48, is critical for cytoprotection and cell proliferation. Here, we reported a novel function for the LGR4 in cholestatic liver injury. In the bile duct ligation (BDL) induced liver injury model, hepatic LGR4 expression was significantly down-regulated. Deficiency of LGR4 in hepatocytes (Lgr4LKO) notably decreased BDL-induced liver injury measured by hepat...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Yuan Gao Wenbo Zhai Lijun Sun Xueqian Du Xianfeng Wang Michael W Mulholland Yue Yin Weizhen Zhang Source Type: research
Protease-activated receptor 2 drives migration in a colon cancer cell line but not in non-cancerous human epithelial cells
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00284.2023. Online ahead of print.ABSTRACTThe inflamed mucosa contains a complex assortment of proteases which may participate in wound healing or in the development of inflammation-associated colon cancer. We sought to determine the role of protease-activated receptor 2 (PAR2) in epithelial wound healing in both untransformed and transformed colonic epithelial cells. Monolayers of primary epithelial cells derived from organoids cultivated from patient colonic biopsies, and of the T84 colon cancer cell line, were grown to confluence, wounded in the pre...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Larissa Lucena P érico Andrew J Vegso Cristiane H Baggio Wallace K MacNaughton Source Type: research
Hepatic LGR4 aggravates cholestasis-induced liver injury in mice
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00127.2023. Online ahead of print.ABSTRACTCurrent therapy for hepatic injury induced by accumulation of bile acid is limited. Leucine-rich repeat G-protein-coupled receptor 4 (LGR4), also known as GPR48, is critical for cytoprotection and cell proliferation. Here, we reported a novel function for the LGR4 in cholestatic liver injury. In the bile duct ligation (BDL) induced liver injury model, hepatic LGR4 expression was significantly down-regulated. Deficiency of LGR4 in hepatocytes (Lgr4LKO) notably decreased BDL-induced liver injury measured by hepat...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Yuan Gao Wenbo Zhai Lijun Sun Xueqian Du Xianfeng Wang Michael W Mulholland Yue Yin Weizhen Zhang Source Type: research
Protease-activated receptor 2 drives migration in a colon cancer cell line but not in non-cancerous human epithelial cells
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00284.2023. Online ahead of print.ABSTRACTThe inflamed mucosa contains a complex assortment of proteases which may participate in wound healing or in the development of inflammation-associated colon cancer. We sought to determine the role of protease-activated receptor 2 (PAR2) in epithelial wound healing in both untransformed and transformed colonic epithelial cells. Monolayers of primary epithelial cells derived from organoids cultivated from patient colonic biopsies, and of the T84 colon cancer cell line, were grown to confluence, wounded in the pre...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Larissa Lucena P érico Andrew J Vegso Cristiane H Baggio Wallace K MacNaughton Source Type: research
Hepatic LGR4 aggravates cholestasis-induced liver injury in mice
Am J Physiol Gastrointest Liver Physiol. 2024 Mar 5. doi: 10.1152/ajpgi.00127.2023. Online ahead of print.ABSTRACTCurrent therapy for hepatic injury induced by accumulation of bile acid is limited. Leucine-rich repeat G-protein-coupled receptor 4 (LGR4), also known as GPR48, is critical for cytoprotection and cell proliferation. Here, we reported a novel function for the LGR4 in cholestatic liver injury. In the bile duct ligation (BDL) induced liver injury model, hepatic LGR4 expression was significantly down-regulated. Deficiency of LGR4 in hepatocytes (Lgr4LKO) notably decreased BDL-induced liver injury measured by hepat...
Source: Am J Physiol Gastroi... - March 5, 2024 Category: Gastroenterology Authors: Yuan Gao Wenbo Zhai Lijun Sun Xueqian Du Xianfeng Wang Michael W Mulholland Yue Yin Weizhen Zhang Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 27. doi: 10.1152/ajpgi.00013.2024. Online ahead of print.ABSTRACTGenetically engineered mouse models play a pivotal role in the modeling of diseases, exploration of gene functions, and the development of novel therapies. In recent years, CRISPR-Cas9-mediated genome editing technology has revolutionized the process of developing such models by enabling precise genome modifications of the multiple interested genes simultaneously. Following genome editing, an efficient genotyping methodology is crucial for subsequent characterization. However, current genotyping methods are la...
Source: Am J Physiol Gastroi... - February 27, 2024 Category: Gastroenterology Authors: Lingxiang Wang Jiale Wang Dongfeng Feng Bin Wang Yasmin Jahan-Mihan Ying Wang Yan Bi Do Young Lim Baoan Ji Source Type: research
The Effects of Corticotropin-Releasing Hormone on Gastric Electrical Activity and Sensorimotor Function in Healthy Volunteers: A Double-blinded Crossover Study
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00298.2023. Online ahead of print.ABSTRACTBiopsychosocial factors are associated with disorders of gut brain interaction and exacerbate gastrointestinal symptoms. The mechanisms underlying pathophysiological alterations of stress remain unclear. Corticotropin-releasing hormone (CRH) is a central regulator of the hormonal stress response and has diverse impact on different organ systems. The aim of the present study was to investigate the effects of peripheral CRH infusion on meal-related gastrointestinal symptoms, gastric electrical activity and gastr...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: I-Hsuan Huang Jolien Schol Stefan Calder Armen A Gharibans Karen Van den Houte Annelies Verheyden Bert Broeders Florencia Carbone Gregory O'Grady Jan Tack Source Type: research
Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00264.2023. Online ahead of print.ABSTRACTRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using l...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: Luis V Herrera-Marcos Roberto Mart ínez-Beamonte Carmen Arnal Cristina Barranquero Juan J Puente-Lanzarote Jos é M Lou-Bonafonte Gonzalo Gonzalo-Romeo Gabriele Mocciaro Benjamin Jenkins Joaquin C Surra Mar ía J Rodríguez-Yoldi V íctor Alastrué-Vera Source Type: research
The Effects of Corticotropin-Releasing Hormone on Gastric Electrical Activity and Sensorimotor Function in Healthy Volunteers: A Double-blinded Crossover Study
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00298.2023. Online ahead of print.ABSTRACTBiopsychosocial factors are associated with disorders of gut brain interaction and exacerbate gastrointestinal symptoms. The mechanisms underlying pathophysiological alterations of stress remain unclear. Corticotropin-releasing hormone (CRH) is a central regulator of the hormonal stress response and has diverse impact on different organ systems. The aim of the present study was to investigate the effects of peripheral CRH infusion on meal-related gastrointestinal symptoms, gastric electrical activity and gastr...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: I-Hsuan Huang Jolien Schol Stefan Calder Armen A Gharibans Karen Van den Houte Annelies Verheyden Bert Broeders Florencia Carbone Gregory O'Grady Jan Tack Source Type: research
