Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00264.2023. Online ahead of print.ABSTRACTRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using l...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: Luis V Herrera-Marcos Roberto Mart ínez-Beamonte Carmen Arnal Cristina Barranquero Juan J Puente-Lanzarote Jos é M Lou-Bonafonte Gonzalo Gonzalo-Romeo Gabriele Mocciaro Benjamin Jenkins Joaquin C Surra Mar ía J Rodríguez-Yoldi V íctor Alastrué-Vera Source Type: research
The Effects of Corticotropin-Releasing Hormone on Gastric Electrical Activity and Sensorimotor Function in Healthy Volunteers: A Double-blinded Crossover Study
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00298.2023. Online ahead of print.ABSTRACTBiopsychosocial factors are associated with disorders of gut brain interaction and exacerbate gastrointestinal symptoms. The mechanisms underlying pathophysiological alterations of stress remain unclear. Corticotropin-releasing hormone (CRH) is a central regulator of the hormonal stress response and has diverse impact on different organ systems. The aim of the present study was to investigate the effects of peripheral CRH infusion on meal-related gastrointestinal symptoms, gastric electrical activity and gastr...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: I-Hsuan Huang Jolien Schol Stefan Calder Armen A Gharibans Karen Van den Houte Annelies Verheyden Bert Broeders Florencia Carbone Gregory O'Grady Jan Tack Source Type: research
Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00264.2023. Online ahead of print.ABSTRACTRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using l...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: Luis V Herrera-Marcos Roberto Mart ínez-Beamonte Carmen Arnal Cristina Barranquero Juan J Puente-Lanzarote Jos é M Lou-Bonafonte Gonzalo Gonzalo-Romeo Gabriele Mocciaro Benjamin Jenkins Joaquin C Surra Mar ía J Rodríguez-Yoldi V íctor Alastrué-Vera Source Type: research
The Effects of Corticotropin-Releasing Hormone on Gastric Electrical Activity and Sensorimotor Function in Healthy Volunteers: A Double-blinded Crossover Study
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00298.2023. Online ahead of print.ABSTRACTBiopsychosocial factors are associated with disorders of gut brain interaction and exacerbate gastrointestinal symptoms. The mechanisms underlying pathophysiological alterations of stress remain unclear. Corticotropin-releasing hormone (CRH) is a central regulator of the hormonal stress response and has diverse impact on different organ systems. The aim of the present study was to investigate the effects of peripheral CRH infusion on meal-related gastrointestinal symptoms, gastric electrical activity and gastr...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: I-Hsuan Huang Jolien Schol Stefan Calder Armen A Gharibans Karen Van den Houte Annelies Verheyden Bert Broeders Florencia Carbone Gregory O'Grady Jan Tack Source Type: research
Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00264.2023. Online ahead of print.ABSTRACTRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using l...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: Luis V Herrera-Marcos Roberto Mart ínez-Beamonte Carmen Arnal Cristina Barranquero Juan J Puente-Lanzarote Jos é M Lou-Bonafonte Gonzalo Gonzalo-Romeo Gabriele Mocciaro Benjamin Jenkins Joaquin C Surra Mar ía J Rodríguez-Yoldi V íctor Alastrué-Vera Source Type: research
The Effects of Corticotropin-Releasing Hormone on Gastric Electrical Activity and Sensorimotor Function in Healthy Volunteers: A Double-blinded Crossover Study
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00298.2023. Online ahead of print.ABSTRACTBiopsychosocial factors are associated with disorders of gut brain interaction and exacerbate gastrointestinal symptoms. The mechanisms underlying pathophysiological alterations of stress remain unclear. Corticotropin-releasing hormone (CRH) is a central regulator of the hormonal stress response and has diverse impact on different organ systems. The aim of the present study was to investigate the effects of peripheral CRH infusion on meal-related gastrointestinal symptoms, gastric electrical activity and gastr...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: I-Hsuan Huang Jolien Schol Stefan Calder Armen A Gharibans Karen Van den Houte Annelies Verheyden Bert Broeders Florencia Carbone Gregory O'Grady Jan Tack Source Type: research
Lipidomic signatures discriminate subtle hepatic changes in the progression of porcine non-alcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 20. doi: 10.1152/ajpgi.00264.2023. Online ahead of print.ABSTRACTRecently, the development of non-alcoholic steatohepatitis (NASH) in common strains of pigs has been achieved using a diet high in saturated fat, fructose, cholesterol and cholate and deficient in choline and methionine. The aim of the present work was to characterize the hepatic and plasma lipidomic changes that accompany the progression of NASH and its reversal by switching pigs back to a chow diet. One month of this extreme steatotic diet was sufficient to induce porcine NASH. The lipidomic platform using l...
Source: Am J Physiol Gastroi... - February 20, 2024 Category: Gastroenterology Authors: Luis V Herrera-Marcos Roberto Mart ínez-Beamonte Carmen Arnal Cristina Barranquero Juan J Puente-Lanzarote Jos é M Lou-Bonafonte Gonzalo Gonzalo-Romeo Gabriele Mocciaro Benjamin Jenkins Joaquin C Surra Mar ía J Rodríguez-Yoldi V íctor Alastrué-Vera Source Type: research
Mechanisms Driving Fasting-Induced Protection from Genotoxic Injury in the Small Intestine
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00126.2023. Online ahead of print.ABSTRACTGenotoxic agents like doxorubicin (DXR) can cause damage to the intestines that can be ameliorated by fasting. How fasting is protective and the optimal timing of fasting and refeeding remain unclear. Here, our analysis of fasting/refeeding-induced global intestinal transcriptional changes revealed metabolic shifts and implicated the cellular energetic hub mTORC1 in protecting from DXR-induced DNA damage. Our analysis of specific transcripts and proteins in intestinal tissue and tissue extracts showed that fas...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Kali Deans-Fielder Timothy Wu Thanh Nguyen Sarah To Yang-Zhe Huang Steven J Bark Jason C Mills Noah F Shroyer Source Type: research
A Mathematical Model of ENaC and Slc26a6 Regulation by CFTR in Salivary Gland Ducts
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00168.2023. Online ahead of print.ABSTRACTCystic Fibrosis (CF) is a genetic disease caused by the mutations of CFTR, the cystic fibrosis transmembrane conductance regulator gene. Cftr is a critical ion channel expressed in the apical membrane of mouse salivary gland striated duct cells. Although Cftr is primarily a Cl- channel, its knockout leads to higher salivary Cl- and Na+concentrations and lower pH. Mouse experiments show that the activation of Cftr up-regulates ENaC (Epithelial Na+Channel) protein expression level and Slc26a6 (a 1Cl- : 2HCO3- ex...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Shan Su Amanda Wahl John Rugis Vinod Suresh David Yule James Sneyd Source Type: research
Mechanisms Driving Fasting-Induced Protection from Genotoxic Injury in the Small Intestine
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00126.2023. Online ahead of print.ABSTRACTGenotoxic agents like doxorubicin (DXR) can cause damage to the intestines that can be ameliorated by fasting. How fasting is protective and the optimal timing of fasting and refeeding remain unclear. Here, our analysis of fasting/refeeding-induced global intestinal transcriptional changes revealed metabolic shifts and implicated the cellular energetic hub mTORC1 in protecting from DXR-induced DNA damage. Our analysis of specific transcripts and proteins in intestinal tissue and tissue extracts showed that fas...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Kali Deans-Fielder Timothy Wu Thanh Nguyen Sarah To Yang-Zhe Huang Steven J Bark Jason C Mills Noah F Shroyer Source Type: research
A Mathematical Model of ENaC and Slc26a6 Regulation by CFTR in Salivary Gland Ducts
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00168.2023. Online ahead of print.ABSTRACTCystic Fibrosis (CF) is a genetic disease caused by the mutations of CFTR, the cystic fibrosis transmembrane conductance regulator gene. Cftr is a critical ion channel expressed in the apical membrane of mouse salivary gland striated duct cells. Although Cftr is primarily a Cl- channel, its knockout leads to higher salivary Cl- and Na+concentrations and lower pH. Mouse experiments show that the activation of Cftr up-regulates ENaC (Epithelial Na+Channel) protein expression level and Slc26a6 (a 1Cl- : 2HCO3- ex...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Shan Su Amanda Wahl John Rugis Vinod Suresh David Yule James Sneyd Source Type: research
Mechanisms Driving Fasting-Induced Protection from Genotoxic Injury in the Small Intestine
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00126.2023. Online ahead of print.ABSTRACTGenotoxic agents like doxorubicin (DXR) can cause damage to the intestines that can be ameliorated by fasting. How fasting is protective and the optimal timing of fasting and refeeding remain unclear. Here, our analysis of fasting/refeeding-induced global intestinal transcriptional changes revealed metabolic shifts and implicated the cellular energetic hub mTORC1 in protecting from DXR-induced DNA damage. Our analysis of specific transcripts and proteins in intestinal tissue and tissue extracts showed that fas...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Kali Deans-Fielder Timothy Wu Thanh Nguyen Sarah To Yang-Zhe Huang Steven J Bark Jason C Mills Noah F Shroyer Source Type: research
A Mathematical Model of ENaC and Slc26a6 Regulation by CFTR in Salivary Gland Ducts
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00168.2023. Online ahead of print.ABSTRACTCystic Fibrosis (CF) is a genetic disease caused by the mutations of CFTR, the cystic fibrosis transmembrane conductance regulator gene. Cftr is a critical ion channel expressed in the apical membrane of mouse salivary gland striated duct cells. Although Cftr is primarily a Cl- channel, its knockout leads to higher salivary Cl- and Na+concentrations and lower pH. Mouse experiments show that the activation of Cftr up-regulates ENaC (Epithelial Na+Channel) protein expression level and Slc26a6 (a 1Cl- : 2HCO3- ex...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Shan Su Amanda Wahl John Rugis Vinod Suresh David Yule James Sneyd Source Type: research
Mechanisms Driving Fasting-Induced Protection from Genotoxic Injury in the Small Intestine
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00126.2023. Online ahead of print.ABSTRACTGenotoxic agents like doxorubicin (DXR) can cause damage to the intestines that can be ameliorated by fasting. How fasting is protective and the optimal timing of fasting and refeeding remain unclear. Here, our analysis of fasting/refeeding-induced global intestinal transcriptional changes revealed metabolic shifts and implicated the cellular energetic hub mTORC1 in protecting from DXR-induced DNA damage. Our analysis of specific transcripts and proteins in intestinal tissue and tissue extracts showed that fas...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Kali Deans-Fielder Timothy Wu Thanh Nguyen Sarah To Yang-Zhe Huang Steven J Bark Jason C Mills Noah F Shroyer Source Type: research
A Mathematical Model of ENaC and Slc26a6 Regulation by CFTR in Salivary Gland Ducts
Am J Physiol Gastrointest Liver Physiol. 2024 Feb 13. doi: 10.1152/ajpgi.00168.2023. Online ahead of print.ABSTRACTCystic Fibrosis (CF) is a genetic disease caused by the mutations of CFTR, the cystic fibrosis transmembrane conductance regulator gene. Cftr is a critical ion channel expressed in the apical membrane of mouse salivary gland striated duct cells. Although Cftr is primarily a Cl- channel, its knockout leads to higher salivary Cl- and Na+concentrations and lower pH. Mouse experiments show that the activation of Cftr up-regulates ENaC (Epithelial Na+Channel) protein expression level and Slc26a6 (a 1Cl- : 2HCO3- ex...
Source: Am J Physiol Gastroi... - February 13, 2024 Category: Gastroenterology Authors: Shan Su Amanda Wahl John Rugis Vinod Suresh David Yule James Sneyd Source Type: research
