Sex differences in zymosan-induced behavioral visceral hypersensitivity and colorectal afferent sensitization
In this study, we systematically assessed sex differences in colorectal neural encoding by conducting high-throughput optical recordings in intact dorsal root ganglia (DRGs) from control and visceral hypersensitive mice. We found an apparent sex difference in zymosan-induced behavioral visceral hypersensitivity: enhanced visceromotor responses to colorectal distension were observed only in male mice, not in female mice. Additionally, a higher number of mechanosensitive colorectal afferents were identified per mouse in the zymosan-treated male group than in the saline-treated male group, whereas the mechanosensitive afferen...
Source: Am J Physiol Gastroi... - December 5, 2023 Category: Gastroenterology Authors: Tiantian Guo Jia Liu Longtu Chen Zichao Bian Guoan Zheng Bin Feng Source Type: research
Sex differences in zymosan-induced behavioral visceral hypersensitivity and colorectal afferent sensitization
In this study, we systematically assessed sex differences in colorectal neural encoding by conducting high-throughput optical recordings in intact dorsal root ganglia (DRGs) from control and visceral hypersensitive mice. We found an apparent sex difference in zymosan-induced behavioral visceral hypersensitivity: enhanced visceromotor responses to colorectal distension were observed only in male mice, not in female mice. Additionally, a higher number of mechanosensitive colorectal afferents were identified per mouse in the zymosan-treated male group than in the saline-treated male group, whereas the mechanosensitive afferen...
Source: Am J Physiol Gastroi... - December 5, 2023 Category: Gastroenterology Authors: Tiantian Guo Jia Liu Longtu Chen Zichao Bian Guoan Zheng Bin Feng Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
Seladelpar combined with complementary therapies improves fibrosis, inflammation and liver injury in a mouse model of nonalcoholic steatohepatitis
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00158.2023. Online ahead of print.ABSTRACTSeladelpar, a selective PPARδ agonist, improves markers of hepatic injury in human liver diseases, but histological improvement of nonalcoholic steatohepatitis (NASH) and liver fibrosis has been challenging with any single agent. To discover how complimentary agents could work with seladelpar to achieve optimal outcomes, this study evaluated a variety of therapeutics (alone and in combination) in a mouse model of NASH. Mice on a high fat Amylin Liver NASH (AMLN) diet were treated for 12 weeks with seladelpar,...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Yun-Jung Choi Jeffrey D Johnson Jin-Ju Lee Jiangao Song Marcy Matthews Marc K Hellerstein Charles A McWherter Source Type: research
Repurposing dried blood spot (DBS) device technology to examine bile acid profiles in human dried fecal spot (DFS) samples
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 28. doi: 10.1152/ajpgi.00188.2023. Online ahead of print.ABSTRACTDried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioa...
Source: Am J Physiol Gastroi... - November 28, 2023 Category: Gastroenterology Authors: Melinda A Engevik Santosh Thapa Ian Lillie Mary Beth Yacyshyn Bruce Yacyshyn Andrew J Percy Donald Chace Thomas D Horvath Source Type: research
The distribution of purinergic receptors, P2Y and P2X, expression within the intestine
Am J Physiol Gastrointest Liver Physiol. 2023 Nov 21. doi: 10.1152/ajpgi.00108.2023. Online ahead of print.ABSTRACTNucleotides are potent extracellular signaling molecules during homeostasis, infection, and injury due to their ability to activate purinergic receptors. The nucleotide ATP activates P2X receptors (P2RXs), while the nucleotides ADP, ATP, UTP and UDP-glucose selectively activate different P2Y receptors (P2RYs). Several studies have established crucial roles for P2 receptors during intestinal inflammatory and infectious diseases, yet the most extensive characterization of purinergic signaling has focused on immu...
Source: Am J Physiol Gastroi... - November 21, 2023 Category: Gastroenterology Authors: Kristen A Engevik Francesca J Scribano J Thomas Gebert Jacob L Perry Sue E Crawford Joseph M Hyser Source Type: research
