Endolysin EN572-5 as an alternative to treat urinary tract infection caused by Streptococcus agalactiae
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-14. doi: 10.1007/s00253-023-12949-8. Epub 2024 Jan 8.ABSTRACTStreptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen causing urinary tract infection (UTI). Endolysin EN572-5 was identified in prophage KMB-572-E of the human isolate Streptococcus agalactiae KMB-572. The entire EN572-5 gene was cloned into an expression vector and the corresponding recombinant protein EN572-5 was expressed in Escherichia coli in a soluble form, isolated by affinity chromatography, and characterized. The isolated protein was highly active after 30 min incubation in a temp...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Maria Kajsikova Michal Kajsik Lucia Bocanova Kristina Papayova Hana Drahovska Gabriela Bukovska Source Type: research
Endolysin EN572-5 as an alternative to treat urinary tract infection caused by Streptococcus agalactiae
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-14. doi: 10.1007/s00253-023-12949-8. Epub 2024 Jan 8.ABSTRACTStreptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen causing urinary tract infection (UTI). Endolysin EN572-5 was identified in prophage KMB-572-E of the human isolate Streptococcus agalactiae KMB-572. The entire EN572-5 gene was cloned into an expression vector and the corresponding recombinant protein EN572-5 was expressed in Escherichia coli in a soluble form, isolated by affinity chromatography, and characterized. The isolated protein was highly active after 30 min incubation in a temp...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Maria Kajsikova Michal Kajsik Lucia Bocanova Kristina Papayova Hana Drahovska Gabriela Bukovska Source Type: research
Spatial profiling of the placental chorioamniotic membranes reveals upregulation of immune checkpoint proteins during Group B Streptococcus infection in a nonhuman primate model
ConclusionDifferential regulation of multiple immune checkpoint proteins in the decidua at the site of a GBS infection indicates a major perturbation in immunologic homeostasis that could benefit the host by restricting immune-driven pathologies or the pathogen by limiting immune surveillance. Protein expression of VISTA, an inhibitory immune checkpoint, was upregulated in the chorion and decidua after GBS infection. Investigating the impact of innate immune cell expression of inhibitory immune checkpoints may reveal new insights into placental host-pathogen interactions at the maternal-fetal interface. (Source: Frontiers ...
Source: Frontiers in cellular and infection microbiology - January 4, 2024 Category: Microbiology Source Type: research
717 Is expectant management of late PPROM an option in patients with GBS colonization?
To determine if carriers of group B streptococcus (GBS) have adverse obstetric and neonatal outcomes when preterm premature rupture of membranes (PPROM) occurs between 34 to 37 weeks of pregnancy. (Source: American Journal of Obstetrics and Gynecology)
Source: American Journal of Obstetrics and Gynecology - January 1, 2024 Category: OBGYN Authors: Elior Eliasi, Miriam Lopian, Ariel Many, Ronnie Cohen, Hadel Shabita, Olena Minich Source Type: research
611 Comparative Effectiveness of Alternate Antibiotic Therapies for Pregnant Patients with Group B Streptococcus Infections
This study evaluates the effectiveness of alternate antibiotic therapies among pregnant patients at an urban safety net hospital. (Source: American Journal of Obstetrics and Gynecology)
Source: American Journal of Obstetrics and Gynecology - January 1, 2024 Category: OBGYN Authors: Emma J. Swayze, Lydia M. Henry, Emily Liske, Erin Dolvin, Alexa Swailes Source Type: research
459 Common vaginal microbes modify Group B Streptococcus growth at epithelial barriers: potential mechanisms governing colonization
Group B Streptococcus (GBS) disease, the leading cause of early-onset neonatal sepsis, is predicated upon vaginal colonization. Given limitations of existing screening and prophylaxis strategies, primary prevention of GBS disease necessitates interventions targeting vaginal colonization. Features of vaginal ecosystems may modify GBS colonization though mechanisms are incompletely understood. We sought to investigate how common vaginal microbes modify GBS growth at epithelial barriers. (Source: American Journal of Obstetrics and Gynecology)
Source: American Journal of Obstetrics and Gynecology - January 1, 2024 Category: OBGYN Authors: Briana Ferguson, Dylan Curry, Elliot Friedman, Jennifer A. McCoy, Paul J. Planet, Dustin Flannery, Karen Puopolo, Lauren Anton, Kristin D. Gerson Source Type: research
A small-scale external quality assessment for PCR detection of group B streptococcus in China
CONCLUSIONS: Our small-scale EQA showed that most participating laboratories have reliable diagnostic capacities for GBS PCR detection. Nonetheless, further improvements in the detection performance of some laboratories are required, particularly with low-concentration samples. Our survey also reinforces the use of EQA as an essential tool to evaluate the overall proficiency of clinical laboratories.PMID:38128816 | DOI:10.1016/j.cca.2023.117733 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 21, 2023 Category: Chemistry Authors: Yingwei Chen Ran Zhao Zhongqiang Huang Chengxiang Chu Yanqun Xiao Xiaobo Hu Xueliang Wang Source Type: research
A small-scale external quality assessment for PCR detection of group B streptococcus in China
CONCLUSIONS: Our small-scale EQA showed that most participating laboratories have reliable diagnostic capacities for GBS PCR detection. Nonetheless, further improvements in the detection performance of some laboratories are required, particularly with low-concentration samples. Our survey also reinforces the use of EQA as an essential tool to evaluate the overall proficiency of clinical laboratories.PMID:38128816 | DOI:10.1016/j.cca.2023.117733 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 21, 2023 Category: Chemistry Authors: Yingwei Chen Ran Zhao Zhongqiang Huang Chengxiang Chu Yanqun Xiao Xiaobo Hu Xueliang Wang Source Type: research
A small-scale external quality assessment for PCR detection of group B streptococcus in China
CONCLUSIONS: Our small-scale EQA showed that most participating laboratories have reliable diagnostic capacities for GBS PCR detection. Nonetheless, further improvements in the detection performance of some laboratories are required, particularly with low-concentration samples. Our survey also reinforces the use of EQA as an essential tool to evaluate the overall proficiency of clinical laboratories.PMID:38128816 | DOI:10.1016/j.cca.2023.117733 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 21, 2023 Category: Chemistry Authors: Yingwei Chen Ran Zhao Zhongqiang Huang Chengxiang Chu Yanqun Xiao Xiaobo Hu Xueliang Wang Source Type: research
Serotype distribution, antimicrobial resistance, and molecular characterization of group B Streptococcus isolates from Chinese pregnant woman
Volume 37, Issue 1, December 2024 . (Source: Journal of Maternal-Fetal and Neonatal Medicine)
Source: Journal of Maternal-Fetal and Neonatal Medicine - December 21, 2023 Category: Perinatology & Neonatology Authors: Yifei ChenLin LiuJing LiuTongzhen JiYang GaoDongmei YangMohan ZhaoYanhong ZhaiZheng Caoa Department of Laboratory Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Beijing Maternal and Child Health Care Hospital, Beijing, C Source Type: research
Development and early testing of a simple, low cost, fast sensor for maternal and neonatal group B Streptococcus
Biosens Bioelectron. 2023 Dec 15;247:115923. doi: 10.1016/j.bios.2023.115923. Online ahead of print.ABSTRACTStreptococcus agalactiae, (Group B Streptococcus (GBS)), is a common colonizer of the female vagina. In women giving birth it can be transmitted to the baby and cause serious illness and even death to the child. We have developed a biosensor comprising of phospholipids and fatty acids vesicles encapsulating high concentration, self-quenched carboxyfluorescein, which is released by the lysis of the vesicle by virulence factors expressed by GBS, becoming diluted and fluorescent. The microbial specificity of the sensor ...
Source: Biosensors and Bioelectronics - December 20, 2023 Category: Biotechnology Authors: Emelie Alsheim Naing Tun Thet Maisem Laabei A Toby A Jenkins Source Type: research
