Use of troponin as a predictor for cardiovascular diseases in patients with type 2 diabetes mellitus.
Abstract People with type 2 diabetes mellitus (T2DM) have two- to four-fold increased cardiovascular mortality in comparison to the general population. With the identification of new therapeutic targets and hypoglycemic drugs for T2DM, the need for a better stratification of CVD risk has emerged to select patients who may need intensive or specific treatment. At present, risk stratification is based on clinical, demographic, and biochemical factors. High sensitivity cardiac troponin (hs-cTn) increases after several ischemic and non-ischemic insults and it is considered a marker of myocardial injury. This review su...
Source: International Journal of Clinical Chemistry - April 14, 2020 Category: Chemistry Authors: Bellia C, Lombardo M, Della-Morte D Tags: Clin Chim Acta Source Type: research

CYC1, SDHA, UQCRC1, UQCRQ, and SDHB might be important biomarkers in kidney transplant rejection.
CONCLUSIONS: We reported several potential genes may be associated with the kidney transplant rejection, which should provide novel biomarkers for kidney transplant surveillance. PMID: 32302684 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 14, 2020 Category: Chemistry Authors: Fan P, Zhang W, Liu Y Tags: Clin Chim Acta Source Type: research

CDT reference values for monitoring chronic alcohol abuse in pregnancy.
CONCLUSIONS: In order to prevent the development of maternal and fetal prenatal alcohol exposure complications, the use of alcohol biomarkers, including CDT, has been proposed. However, this biomarker, in the monitoring of alcohol use in pregnancy, has so far been applied adopting the same cut-off used for general population without taking into consideration the progressive physiological increase of its value throughout the pregnancy. In the present study, a specific re-assessment of the CDT reference concentrations of each trimester is reported. PMID: 32302685 [PubMed - as supplied by publisher] (Source: Internationa...
Source: International Journal of Clinical Chemistry - April 14, 2020 Category: Chemistry Authors: Bortolotti F, Raffaelli R, Di Simone N, Semprebon M, Mirandola M, Simonetto C, De Marchi F, Trevisan MT, Carli G, Dorizzi RM, Scambia G, Franchi M, Tagliaro F Tags: Clin Chim Acta Source Type: research

Diagnosis of Late-Infantile Neuronal Ceroid Lipofuscinosis using dried blood spot-based assay for TPPI enzyme activity.
CONCLUSION: A sequence variant causing Arg339Gln substitution in a proband had 12% TPPI. TPPI activity can be reliably measured in DBS, giving an opportunity to diagnose NCL2 at birth and refer patients for enzyme replacement or other therapies for earliest intervention, or alternatively offers a second-tier confirmatory test. PMID: 32298681 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 13, 2020 Category: Chemistry Authors: Gavin M, Khatoon S, Marchi EJ, Mevs CA, Bolton DC, Velinov MT, Junaid MA Tags: Clin Chim Acta Source Type: research

A new role for dolichol isoform profile in the diagnostics of CDG disorders.
Abstract Dolichol is a membrane lipid which carries monosaccharides and glycans for N-linked protein glycosylation occurring in the endoplasmic reticulum. Recently, some types of congenital disorders of glycosylation (CDG) have been described as consequences of defects in dolichol biosynthesis and metabolism, yet these types of CDG are not detectable by standard screening methods. The aim of this project was to evaluate the potential of dolichol as a biomarker of CDG. Biological material for this study consisted of urine samples from 75 controls, 6 patients with CDG and 43 patients with suspicion of CDG; samples o...
Source: International Journal of Clinical Chemistry - April 11, 2020 Category: Chemistry Authors: Zdrazilova L, Kuchar L, Ondruskova N, Honzik T, Hansikova H Tags: Clin Chim Acta Source Type: research

Adipokines as novel biomarkers of cardio-metabolic disorders.
Abstract Obesity, defined as having a body mass index (BMI) greater than 30 kg/m2, has been verified to be associated with several health problems which are always grouped as metabolic syndrome. However, the underlying pathogenic mechanisms remain unclear. The hallmarks of obesity are dysfunctional changes in adipose tissue and dyslipidemia characterized by elevated serum levels of low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C), and reduced serum levels of high density lipoprotein cholesterol (HDL-C). Currently, it is widely accepted that rather than being a reservoi...
Source: International Journal of Clinical Chemistry - April 10, 2020 Category: Chemistry Authors: Su X, Peng D Tags: Clin Chim Acta Source Type: research

A novel mutation within intron 17 of the CUL7 gene results in appearance of premature termination codon.
Abstract A couple with five adverse pregnancy history required prenatal diagnosis. The fetus of this study was their fifth pregnancy. The fetus was found NT thickening at 12 weeks and 4 days gestation and the average long bone of limbs retardation 4SD at 27 weeks and 4 days gestation. Karyotype was normal. The next-generation sequencing (NGS) and Sanger sequencing were conducted of this fetus. The compound heterozygous mutations c.3722_3749dup[p.V1252fs*23] and c.3355+5 G>A at CUL7 gene were detected. The mutation c.3355+5 G>A was a novel mutation within intron 17 of the CUL7 gene. Minigene array was used to...
Source: International Journal of Clinical Chemistry - April 9, 2020 Category: Chemistry Authors: Guo L, Feng Z, Jin X, Yin S, Zhang M, Gao Y, Zhang B, Wang H, Liu L Tags: Clin Chim Acta Source Type: research

Identification of a novel homozygous nonsense mutation in the CDHR1 gene in a Chinese family with autosomal recessive retinitis pigmentosa.
CONCLUSION: Our study expanded the CDHR1 mutation spectrum of RP in the Chinese population, which might help to better understand RP molecular pathogenesis. PMID: 32277948 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 8, 2020 Category: Chemistry Authors: Gan L, Yang C, Shu Y, Liu F, Sun R, Deng B, Xu J, Huang G, Qu C, Gong B, Li J Tags: Clin Chim Acta Source Type: research

ELISA-based detection of open reading frame protein 1 in patients at risk of developing lung cancer.
CONCLUSION: ORF1p can be used to identify patients at high risk of developing lung cancer and may provide an effective, non-invasive risk assessment marker to complement LDCT screening. PMID: 32275987 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 7, 2020 Category: Chemistry Authors: Sharp CN, Korte EA, Hosseinejad K, Pitman J, Lavasanifar A, Eichenberger DJ, Sephton S, Cash E, Jortani SA Tags: Clin Chim Acta Source Type: research

Serum Sitosterol Level Predicting ABCG5 or ABCG8 Genetic Mutations.
CONCLUSION: We suggest a cutoff value of sitosterol 15 μg/ml that shows higher positive predictive value than 10 μg/ml. PMID: 32275988 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 7, 2020 Category: Chemistry Authors: Kojima N, Tada H, Usui S, Sakata K, Hayashi K, Nohara A, Inazu A, Takamura M, Kawashiri MA Tags: Clin Chim Acta Source Type: research

Bone, inflammation and chronic kidney disease.
Abstract Increasing knowledge on inflammatory mediators and bone metabolism highlights the relationship between inflammation and bone disease. During acute illness, inflammatory cells and cytokines modulate bone cells activity so as to mobilize calcium seemingly to supply the metabolic requirements for immune response. In case of long lasting, chronic inflammatory states a condition of maladaptive, smouldering inflammation is realized and negatively affects calcium bone balance. Aging, now nicknamed inflammaging, is regarded as a chronic inflammatory condition, characterized by increased circulating inflammatory c...
Source: International Journal of Clinical Chemistry - April 7, 2020 Category: Chemistry Authors: Sandro M, Martini Natalia, Silverio R, Lida T, Pablo UT, Jordi B, Marzia P, ERA-EDTA Working Group on CKD-MBD Tags: Clin Chim Acta Source Type: research

Development of blood-based biomarker tests for early detection of colorectal neoplasia: Influence of blood collection timing and handling procedures.
en HJ Abstract INTRODUCTION: Blood-based, cancer-associated biomarkers are susceptible to a variety of well-known preanalytical factors. The influence of bowel preparation before a diagnostic colonoscopy on biomarker levels is, however, poorly investigated. The present study assessed the influence of bowel preparation on colorectal cancer-associated biomarkers. In addition, the effect of single versus double centrifugation of plasma biomarkers was assessed. METHODS: Blood samples were collected pre- and post-bowel preparation from 125 subjects scheduled for first time diagnostic colonoscopy due to symptoms at...
Source: International Journal of Clinical Chemistry - April 6, 2020 Category: Chemistry Authors: Lech Pedersen N, Mertz Petersen M, Ladd JJ, Lampe PD, Bresalier RS, Davis GJ, Demuth C, Jensen SØ, Andersen CL, Ferm L, Jarle Christensen I, Nielsen HJ Tags: Clin Chim Acta Source Type: research

Research status and perspectives for pathogenic spirochete vaccines.
Abstract Leptospira interrogans, Borrelia burgdorferi, and Treponema pallidum are important pathogenic spirochetes. The incidence of human diseases caused by pathogenic spirochetes, e.g., leptospirosis, Lyme disease, and syphilis, has been recently increasing, posing a threat to public health. Mechanisms of spirochete pathogenicity are not yet fully understood, and no safe and effective vaccine to prevent and control the infection by pathogenic spirochetes is currently available.In this article, we review the progress of research into the pathogenic spirochete vaccine, mainly in terms of vaccine types.The developm...
Source: International Journal of Clinical Chemistry - April 6, 2020 Category: Chemistry Authors: Duan J, Zhao Y, Zhang X, Jiang H, Xie B, Zhao T, Zhao F Tags: Clin Chim Acta Source Type: research

Clinical Utility of Serum IgG4 Measurement.
This article will review the structure and function of IgG4, methods of measuring serum IgG4 concentrations, clinical conditions associated with increased and decreased serum IgG4, and the test characteristics of serum IgG4 in the diagnosis and management of Immunoglobulin G4-Related Disease (IgG4-RD). The four subclasses of IgG were discovered in 1964 through experiments on monoclonal IgG in patients with myeloma. Since 2001, interest in measuring serum IgG subclasses has increased dramatically due to the emergence of IgG4-RD, a multisystem fibroinflammatory condition wherein polyclonal serum IgG4 concentration is increas...
Source: International Journal of Clinical Chemistry - April 6, 2020 Category: Chemistry Authors: Varghese JL, Fung AWS, Mattman A, Quach TTT, Gauiran DTV, Carruthers MN, Chen LYC Tags: Clin Chim Acta Source Type: research

The diagnostic accuracy of Maternal Serum Alpha-Fetoprotein Variants (AFP-L2 and AFP-L3) in predicting Fetal Open Neural Tube Defects and Abdominal Wall Defects.
Abstract INTRODUCTION: To evaluate a risk model of the maternal serum α-fetoprotein variants L2 and L3 (AFP-L2 and AFP-L3) for predicting fetal open neural tube defects (ONTD) and abdominal wall defects (AWD). METHODS: Subjects were divided into the ONTD group (21), the AWD group (16) and the control group (38). Comparative analysis was performed using a risk model, receiver operating characteristic (ROC) curves and area under the curve (AUC) were used for screening performance of AFP-L2 and AFP-L3. RESULTS: AFP-L2 and AFP-L3 concentrations in the ONTD group and the AWD group were both significantl...
Source: International Journal of Clinical Chemistry - April 3, 2020 Category: Chemistry Authors: Chen Y, Wang X, Lu S, Huang J, Zhang L, Hu W Tags: Clin Chim Acta Source Type: research

Serum alkaline phosphatase level and metabolic syndrome.
PMID: 32243983 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 31, 2020 Category: Chemistry Authors: Kawada T Tags: Clin Chim Acta Source Type: research

Glycoprotein biomarkers and analysis in chronic obstructive pulmonary disease and lung cancer with special focus on serum immunoglobulin G.
Abstract Chronic obstructive pulmonary disease (COPD) and lung cancer are two major diseases of the lung with high rate of mortality, mostly among tobacco smokers. The glycosylation patterns of various plasma proteins show significant changes in COPD and subsequent hypoxia, inflammation and lung cancer, providing promising opportunities for screening aberrant glycan structures contributing to early detection of both diseases. Glycoproteins associated with COPD and lung cancer consist of highly sialylated N-glycans, which play an important role in inflammation whereby hypoxia leads to accumulation of N-glycolyl sia...
Source: International Journal of Clinical Chemistry - March 31, 2020 Category: Chemistry Authors: Komaromy A, Reider B, Jarvas G, Guttman A Tags: Clin Chim Acta Source Type: research

The metabolism of bile acid and amino acids is correlated with the diagnosis of pancreatic cancer.
CONCLUSION: Bile acids (especially taurocholic acid) performed to be potential biomarkers to diagnose PC. And other amino acid (such as L-glutamine, glutamic acid, L-phenylalanine, L-tryptophan, and L-arginine) in the serum samples of PC might provide a sensitive, blood-borne diagnostic signature for the presence of PC or its precursor lesions. PMID: 32243985 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 31, 2020 Category: Chemistry Authors: Xiong Y, Shi C, Liu X, Yang P Tags: Clin Chim Acta Source Type: research

Usefulness of serum glucose and potassium ratio as a predictor for 30-day death among patients with severe traumatic brain injury.
CONCLUSIONS: Serum GPR in cases of sTBI is substantially associated with trauma severity and 30-day mortality. Therefore, the potential value of serum GPR for predicting short-term mortality of sTBI patients is favorable. PMID: 32240656 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 30, 2020 Category: Chemistry Authors: Zhou J, Yang CS, Shen LJ, Lv QW, Xu QC Tags: Clin Chim Acta Source Type: research

Relationships among erythrocyte-derived microRNAs in serum of healthy donors.
CONCLUSION: There are significant associations among serum levels of erythrocyte-derived miRNAs, and these associations should be taken into account when considering the miRNAs as disease biomarkers. PMID: 32240657 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 30, 2020 Category: Chemistry Authors: Wakabayashi I, Sotoda Y, Eguchi R Tags: Clin Chim Acta Source Type: research

Less is more: Two automated interventions to increase vitamin B12 measurement when long-term proton pump inhibitor and decrease redundant testing.
rking group Abstract INTRODUCTION: To test the efficacy of two interventions to promote the measurement of serum vitamin B12 (s- vitamin B12) in patients with long-term proton pump inhibitor (PPI) use and to decrease inadequate s-vitamin B12 measurement by implementing a minimum retest interval. MATERIAL AND METHODS: The Laboratory Information System (LIS) automatically registered s-vitamin B12 when not ordered by General Practitioners to all requests patients with long term (>3years) PPI treatment, and with no s-vitamin B12 testing in the previous year. Through the second intervention, the LIS reported th...
Source: International Journal of Clinical Chemistry - March 29, 2020 Category: Chemistry Authors: Salinas M, López-Garrigós M, Flores E, Blasco A, Leiva-Salinas C, PRIMary Care-LABoratory (PRIMLAB) working group Tags: Clin Chim Acta Source Type: research

A Case of Unexplained Duodenal Ulcer and Massive Gastrointestinal Bleed.
Abstract A 73-year-old man was displaying symptoms of massive gastrointestinal (GI) bleed. Surgical actions were performed to control the bleed caused by an erosive duodenal ulcer with duodenal perforation. When investigating the culprit of this case, the pain medications prescribed two weeks prior by a traditional Chinese medicine doctor raised attention. The patient's admission serum sample and the pain medications from unknown sources were analyzed using a clinically validated liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method. The NSAIDs diclofenac, piroxicam, and indomethacin were identi...
Source: International Journal of Clinical Chemistry - March 29, 2020 Category: Chemistry Authors: Ruben Luo Y, Goodnough R, Menza R, Badea A, Yiu-Wei Luu H, Kornblith LZ, Lynch KL Tags: Clin Chim Acta Source Type: research

Six novelMutation analysis of the androgen receptor gene in 17 Chinese patients with androgen insensitivity syndrome.
CONCLUSION: Our study expands the spectrum of AR mutations and could provide evidence for the genetic and reproductive counseling of families with AIS. All of these findings broadened the mutation spectrum of AR, which were significantly valuable for patient gender assignment, genetic counseling and the clinical and psychological management. PMID: 32229106 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 27, 2020 Category: Chemistry Authors: Jiang X, Teng Y, Chen X, Liang N, Li Z, Liang D, Wu L Tags: Clin Chim Acta Source Type: research

Dynamic change process of target genes by RT-PCR testing of SARS-Cov-2 during the course of a Coronavirus Disease 2019 patient.
We report the dynamic change process of target genes by RT-PCR testing of SARS-Cov-2 during the course of a COVID-19 patient: from successive negative results to successive single positive nucleocapsid gene, to two positive target genes (orf1ab and nucleocapsid) by RT-PCR testing of SARS-Cov-2, and describe the diagnosis, clinical course, and management of the case. In this case, negative results of RT-PCR testing was not excluded to diagnose a suspected COVID-19 patient, clinical signs and symptoms, other laboratory findings, and chest CT images should be taken into account for the absence of enough positive evidence. Thi...
Source: International Journal of Clinical Chemistry - March 27, 2020 Category: Chemistry Authors: Lv DF, Ying QM, Weng YS, Shen CB, Chu JG, Kong JP, Sun DH, Gao X, Weng XB, Chen XQ Tags: Clin Chim Acta Source Type: research

Role of oral microbiota in atherosclerosis.
Abstract Oral infections are common among individuals of all ages and can activate local and systemic inflammation. The inflammatory response plays an important role in atherosclerosis. An increasing number of studies have reported an association between oral pathogen infection and atherosclerotic coronary heart disease. For instance, epidemiological studies support the positive correlation between oral infections and atherosclerosis. The presence of oral pathogens in human atherosclerotic plaques has been detected by multiple methods, and oral infections promote atherosclerosis in animal experiments. Various mech...
Source: International Journal of Clinical Chemistry - March 24, 2020 Category: Chemistry Authors: Liu XR, Xu Q, Xiao J, Deng YM, Tang ZH, Tang YL, Liu LS Tags: Clin Chim Acta Source Type: research

The host's angiotensin-converting enzyme polymorphism may explain epidemiological findings in COVID-19 infections.
PMID: 32220422 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 24, 2020 Category: Chemistry Authors: Delanghe JR, Speeckaert MM, De Buyzere ML Tags: Clin Chim Acta Source Type: research

Identification of Clinically Relevant Variants by Whole Exome Sequencing in Chinese Patients with Sporadic Non-syndromic Type A Aortic Dissection.
CONCLUSIONS: The variants identified in our research might not only result in the occurrence of ATAAD, but also add complexities and difficulties to the clinical practice. Our data demonstrated that WES was an effective tool for determining genetic etiologies of non-syndromic ATAAD and could be helpful in genetic counseling for ATAAD patients and their at-risk family members. PMID: 32209317 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 21, 2020 Category: Chemistry Authors: Zhao H, Yang Y, Pan X, Li W, Sun L, Guo J Tags: Clin Chim Acta Source Type: research

More Accurate LDL-C Calculation: Externally Validated, Guideline Endorsed.
Abstract Low density lipoprotein-cholesterol (LDL-C) serves as the primary target of therapy for preventing atherosclerotic cardiovascular disease (ASCVD). Recently released European and American guidelines on lipid management recommend attaining very low LDL-C levels (
Source: International Journal of Clinical Chemistry - March 20, 2020 Category: Chemistry Authors: Brownstein AJ, Martin SS Tags: Clin Chim Acta Source Type: research

Age-specific reference intervals for anti-M üllerian hormone in Chinese boys: a population-based study.
Age-specific reference intervals for anti-Müllerian hormone in Chinese boys: a population-based study. Clin Chim Acta. 2020 Mar 18;: Authors: Wang J, Jiang L, Zhang X, Gong S, Chen M, Hu X, Yao H Abstract BACKGROUND: Anti-Müllerian hormone (AMH) is used for evaluating gonadal development and testicular function. We aimed to establish AMH reference intervals and to determine the correlations between AMH level and age, body mass index (BMI), and follicle stimulating hormone (FSH), luteinizing hormone (LH), and total testosterone (TT) levels in healthy Chinese boys. METHODS: Serum AMH leve...
Source: International Journal of Clinical Chemistry - March 18, 2020 Category: Chemistry Authors: Wang J, Jiang L, Zhang X, Gong S, Chen M, Hu X, Yao H Tags: Clin Chim Acta Source Type: research

The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study.
PMID: 32199785 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 18, 2020 Category: Chemistry Authors: Sharma TK, Vardey SK Tags: Clin Chim Acta Source Type: research

A high performance liquid chromatography fluorescence method for the analysis of both pyridoxal-5-phosphate and thiamine pyrophosphate in whole blood.
CONCLUSIONS: Overall, this method provides an economical alternative in routine clinical diagnostic laboratories wishing to perform P5P and TPP analysis. PMID: 32197927 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 17, 2020 Category: Chemistry Authors: Long Nguyen V, Darman M, Ireland A, Fitzpatrick M Tags: Clin Chim Acta Source Type: research

The percentage of non-oxidized PTH concentration remains stable over a period of 1 year in hemodialyzed patients.
CONCLUSIONS: The percentage of n-ox PTH is stable over time in stable HD patients. PMID: 32194038 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 16, 2020 Category: Chemistry Authors: Cavalier E, Lukas P, Warling X, Moonen M, Smelten N, Delanaye P Tags: Clin Chim Acta Source Type: research

A novel SYNE2 mutation identified by whole exome sequencing in a Korean family with Emery-Dreifuss Muscular Dystrophy.
CONCLUSION: To the best knowledge, SYNE2 mutation was reported first by whole exome sequencing in a Korean family with EDMD-like features. We emphasized the role of genetic analysis using whole exome sequencing, which allows the correct recognition of this molecular diagnosis and brings together the neuromuscular spectrum of this complex clinical scenario, leading to proper clinical management. PMID: 32184094 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 14, 2020 Category: Chemistry Authors: Joung Lee S, Lee S, Choi E, Shin S, Park J Tags: Clin Chim Acta Source Type: research

COX-2 in liver fibrosis.
Abstract As a vital inducible sensor, cyclooxygenase-2 (COX-2) plays an important role in the progress of hepatic fibrogenesis. Activation of hepatic stellate cells (HSCs) in the liver can significantly accelerate the onset and development of liver fibrosis. COX-2 overexpression triggers inflammation that is an important inducer in hepatic fibrosis. Increasing evidence indicates that COX-2 is involved in the main pathogenesis of liver fibrosis, such as inflammation, apoptosis, and cell senescence. Moreover, COX-2 expression is altered in patients and animal models with non-alcoholic fatty liver disease or cirrhosi...
Source: International Journal of Clinical Chemistry - March 14, 2020 Category: Chemistry Authors: Yang H, Xuefeng Y, Shandong W, Jianhua X Tags: Clin Chim Acta Source Type: research

Thrombocytopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: A meta-analysis.
CONCLUSIONS: Low platelet count is associated with increased risk of severe disease and mortality in patients with COVID-19, and thus should serve as clinical indicator of worsening illness during hospitalization. PMID: 32178975 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 13, 2020 Category: Chemistry Authors: Lippi G, Plebani M, Michael Henry B Tags: Clin Chim Acta Source Type: research

Serum D-dimer as a diagnostic index of PJI and retrospective analysis of etiology in patients with PJI.
CONCLUSION: Patients with PJI have high levels of serum D-dimer, which is a promising marker for the diagnosis of PJI. The Gram-positive bacteria are major pathogen in PJI after arthroplasty, and Staphylococcus aureus is the most common organism. Clinical efficacy is significantly improved by reasonable choice of antibiotics and effective medicine education. PMID: 32178976 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 13, 2020 Category: Chemistry Authors: Hu Q, Fu Y, Tang L Tags: Clin Chim Acta Source Type: research

Oxidation of Parathyroid Hormone.
Abstract Parathyroid hormone (PTH) is the key hormone regulating calcium homeostasis and, as such, is an important diagnostic and prognostic marker. Although the measurement of PTH has greatly improved over the past few decades, oxidation status thereof is unaccounted for in currently used assays. For example, PTH can be oxidized on methionine residues located at amino acid positions 8 and 18. This post-translational modification is relevant because it results in PTH refolding and decreased activation of the PTH1 receptor. Although loss of activity after oxidation was observed as early as 1934, only recently a met...
Source: International Journal of Clinical Chemistry - March 13, 2020 Category: Chemistry Authors: Ursem SR, Vervloet MG, de Jongh RT, Heijboer AC Tags: Clin Chim Acta Source Type: research

Clinical and pathological features and varied mutational spectra of pathogenic genes in 55 Chinese patients with nephronophthisis.
CONCLUSION: A high novel mutation rate was identified, and disease-causing mutations of NPHP3 prevailed in this group of Chinese NPHP patients. This is the second report of a patient with CEP83 mutations. PMID: 32173348 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 12, 2020 Category: Chemistry Authors: Yue Z, Lin H, Li M, Wang H, Liu T, Hu M, Chen H, Tong H, Sun L Tags: Clin Chim Acta Source Type: research

The association and discordance between glycated hemoglobin A1c and glycated albumin, assessed using a blend of multiple linear regression and random forest regression.
CONCLUSIONS: HbA1c and GA values are inconsistent in the population we studied. A model that blends MLR and RFR can be used to correct HbA1c values when conflicting HbA1c and GA values are encountered in patients. PMID: 32169421 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 10, 2020 Category: Chemistry Authors: Zeng Y, He H, Zhou J, Zhang M, Huang H, An Z Tags: Clin Chim Acta Source Type: research

Molecular diagnosis of dystrophinopathies in Morocco and report of six novel mutations.
We report here, molecular analysis results of dystrophin gene during 27 years in a large Moroccan cohort of 356 patients, using the multiplex polymerase chain reaction (MPCR) to screen for hot-spot exon deletions. First applications of whole dystrophin gene sequencing in our lab lead to the identification of six novel mutations. PMID: 32169422 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 10, 2020 Category: Chemistry Authors: El Kadiri Y, Selouani Y, Ratbi I, Lyahyai J, Zrhidri A, Sahli M, Ouhenach M, Cherkaoui Jaouad I, Sefiani A, Sbiti A Tags: Clin Chim Acta Source Type: research

High risk screening for Gaucher disease in patients with splenomegaly and/or thrombocytopenia in China: 55 cases identified.
Abstract Gaucher disease (GD) is a common lysosomal storage disorder caused by deficiency of glucocerebrosidase (GCase) due to the pathogenic variants in the GBA gene. The aim of this study was to evaluate the performance of high risk screening program for GD by measuring the enzyme activities of GCase and chitotriosidease in dried blood spots of patients with splenomegaly and/or thrombocytopenia. A total of 787 subjects (364 females and 423 males) with unexplained splenomegaly and/or thrombocytopenia were enrolled in this study from May 2016 to Aug 2019. The cutoff value of GCase activity was set as less than 3.0...
Source: International Journal of Clinical Chemistry - March 9, 2020 Category: Chemistry Authors: Huang Y, Jia X, Tang C, Liu S, Sheng H, Zhao X, Zeng C, Liu L Tags: Clin Chim Acta Source Type: research

Identification of novel missense mutations associated with non-syndromic syndactyly in two vietnamese trios by whole exome sequencing.
CONCLUSIONS: The result suggested that the two novel mutations may be pathogenic for the disease in these families under the dominant model, provided the initial data for further functional studies to investigate whether those mutations play a disturbing role in the molecular network of syndactyly. PMID: 32165123 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 9, 2020 Category: Chemistry Authors: Thy Ngoc N, Thuy Duong N, Hai Quynh D, Dang Ton N, Hai Duc H, Thi Minh Huong L, Thi Lan Anh L, Van Hai N Tags: Clin Chim Acta Source Type: research

Data mining: Seasonal fluctuations and associations between thyroid stimulating hormone and lipid profiles.
In this study, we aimed to evaluate seasonal variations in TSH and lipid profiles based on clinical big data. METHOD: This observational, retrospective big data study enrolled a total of 20,192 individuals who visited Peking Union Medical College Hospital for routine health check-ups from 2014 to 2018. Demographic, medical history, common biochemical analytes, and thyroid related test data were obtained. A Kruskal-wallis analysis was used to compare the differences in total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) by TSH quartil...
Source: International Journal of Clinical Chemistry - March 9, 2020 Category: Chemistry Authors: Wang D, Yu S, Zou Y, Li H, Cheng X, Xu T, Qiu L Tags: Clin Chim Acta Source Type: research

Do Cytokines Correlate with Refractory Kawasaki Disease in Children?
Abstract BACKGROUND: Kawasaki disease(KD)is the most common type of pediatric vasculitis. Ten to twenty percent of children with KD do not respond to initial intravenous immunoglobulin (IVIG) treatment which called refractory Kawasaki Disease. If untreated, approximately 15% to 25% of KD patients have complications. Therefore, it is important to predict whether KD is resistant to IVIG at an early stage. We determined whether cytokines are predictors of refractory Kawasaki Disease in children. METHODS: We retrospectively reviewed the medical records of 265 children diagnosed with KD who received IVIG within 10...
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Wang Y, Yun Qian S, Yuan Y, Wang Q, Gao L, Chen X, Yu X, Zhen Z Tags: Clin Chim Acta Source Type: research

Prognostic value of combined preoperative fibrinogen-albumin ratio and platelet-lymphocyte ratio score in patients with breast cancer:a prognostic nomogram study.
Abstract OBJECTIVE: The purpose of this study was to evaluate the prognostic value of combined preoperative fibrinogen-albumin ratio and platelet-lymphocyte ratio score (FAR-PLR score) in breast cancer, and to establish a nomogram based on the score as well as clinicopathological factors to predict the prognosis of breast cancer. METHODS: The study cohort included 707 breast cancer patients who underwent curative resection in Taizhou Hospital of Zhejiang Province, China from January 2010 to April 2016. FAR and PLR increased by 2 at the same time, only one index increased by 1, and none increased by 0. The rel...
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Zheng Y, Wu C, Yan H, Chen S Tags: Clin Chim Acta Source Type: research

Fasting glucose-to-HbA1c ratio is a good indicator of G6PD deficiency, but not thalassemia, in patients with type 2 diabetes mellitus.
Abstract AIMS: Patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency or thalassemia have a shorter red blood cell lifespan; therefore, HbA1c is underestimated in these patients. To address these issues, we sought an early indicator for G6PD deficiency or thalassemia in DM patients. METHODS: A total of 4,908 patients with DM and 1848 subjects without DM were included in this study. Fasting glucose (FG) levels, HbA1c levels, hemogram profiles and G6PD activities were measured. Genotypic analyses of G6PD deficiency and thalassemia were performed. RESULTS: DM patients with G6PD deficiency had sig...
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Chang YS, Hsiao LY, Lin CY, Shih MC, Hsieh MC, Chang JG Tags: Clin Chim Acta Source Type: research

Serum haptoglobin correlates positively with cholesterol and triglyceride concentrations in an obese Mongolian population.
Abstract BACKGROUND: Recent studies revealed that several genetic polymorphisms of haptoglobin gene (HP) and the haptoglobin-related protein gene (HPR) associated not only with haptoglobin (HP) but total, non-HDL, and/or LDL cholesterol concentrations in various populations. METHODS: Association between serum HP concentrations and polymorphisms of HP and the HPR gene, or anthropometric and metabolic factors were examined in Mongolian participants (n=927) using linear regression analyses. RESULTS: The association of HP and HPR polymorphisms with serum HP concentration but not serum lipids concentrations w...
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Soejima M, Munkhtulga L, Furukawa K, Iwamoto S, Koda Y Tags: Clin Chim Acta Source Type: research

Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020.
CONCLUSIONS: Therefore, we concluded that viral NAT played an important role in identifying SARS-CoV-2 infection. PMID: 32156607 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Liu R, Han H, Liu F, Lv Z, Wu K, Liu Y, Feng Y, Zhu C Tags: Clin Chim Acta Source Type: research

FGF23 measurement in chronic kidney disease: What is it really reflecting?
Abstract Fibroblast growth factor can be measured in clinical practice using ELISA, with acceptable validity. Different from many metabolites and minerals, its value can differ by a thousand-fold between individuals, largely because of differences in kidney function and dietary habits. This wide range complicates the proper interpretation of the concentration of FGF23, both in terms of the appropriateness of a given value for a given estimated GFR, and in terms of estimating the magnitude of risk for clinical events, with which FGF23 is clearly associated. In this narrative review, the impact of kidney function, e...
Source: International Journal of Clinical Chemistry - March 7, 2020 Category: Chemistry Authors: Vervloet MG Tags: Clin Chim Acta Source Type: research

The endothelin system as target for therapeutic interventions in cardiovascular and renal disease.
Abstract Endothelins including its most abundant isoform, endothelin-1 (ET-1), are peptides acting as vasoconstrictors when binding to ETA and ETB receptors, and, in addition to their distinct roles in normal physiology, endothelins have a central role in the pathophysiology of many diseases including cardiovascular and renal diseases. Endothelin-1 (ET-1), the most potent vasoconstrictor in the cardiovascular system, regulates basal vascular tone and glomerular hemodynamics. ET-1 is involved also in vascular and cardiac hypertrophy, inflammation, and in the development and progression of cardiovascular diseases - ...
Source: International Journal of Clinical Chemistry - March 6, 2020 Category: Chemistry Authors: Eroglu E, Kocyigit I, Lindholm B Tags: Clin Chim Acta Source Type: research