Procalcitonin: Between evidence and critical issues.
Abstract Sepsis is a life-threatening organ dysfunction caused by a dysregulated response of the host to infection. It represents one of the major health care problems worldwide. Unfortunately, the diagnosis of sepsis is challenging for many reasons, including a lack of a sufficiently sensitive and specific diagnostic test. When procalcitonin (PCT) was discovered, it was thought that it could become the best test for identifying patients with sepsis. From the evidence sources in the available literature, it is now clear that the power of PCT in differentiating infectious from non-infectious forms of systemic infla...
Source: International Journal of Clinical Chemistry - June 10, 2019 Category: Chemistry Authors: Aloisio E, Dolci A, Panteghini M Tags: Clin Chim Acta Source Type: research

Recommendations for laboratory informatics specifications needed for the application of patient-based real time quality control.
Abstract Patient based real time Quality Control (PBRTQC) algorithms provide many advantages over conventional QC approaches including lower cost, absence of commutability problems, continuous real-time monitoring of performance, and sensitivity to pre-analytical error. However, PBRTQC is not as simple to implement as conventional QC because of the requirement to access patient data as well as setting up appropriate rules, action protocols, and choosing best statistical algorithms. These requirements need capable and flexible laboratory informatics (middleware). In this document, the necessary features of software...
Source: International Journal of Clinical Chemistry - June 10, 2019 Category: Chemistry Authors: Loh TP, Cervinski MA, Katayev A, Bietenbeck A, van Rossum H, Badrick T, International Federation of Clinical Chemistry and Laboratory Medicine Committee on Analytical Quality Tags: Clin Chim Acta Source Type: research

A homozygous ABCB4 mutation causing an LPAC syndrome evolves into cholangiocarcinoma.
Abstract Low phospholipid-associated cholelithiasis (LPAC) is characterized by the association of ABCB4 mutations and low biliary phospholipid concentration with symptomatic and recurring cholelithiasis. In the present study, we reported a case of a 63-year-old woman, who presented a biliary pain beginning at the age of 30, recurrent after cholecystectomy, along with "comet-tail shadows" revealed by ultrasonography thus, fulfilling the diagnosis of LPAC. This disease evolved into a cholangiocarcinoma. To understand the molecular basis of this phenotype, we performed the ABCB4 gene sequencing, followed by...
Source: International Journal of Clinical Chemistry - June 7, 2019 Category: Chemistry Authors: Khabou B, Trigui A, Boudawara TS, Keskes L, Kamoun H, Barbu V, Fakhfakh F Tags: Clin Chim Acta Source Type: research

Quantification of NETs formation in neutrophil and its correlation with the severity of sepsis and organ dysfunction.
CONCLUSION: NETs formation is significantly higher due to which it is contributing to the sepsis severity and organ failure. PMID: 31181192 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - June 7, 2019 Category: Chemistry Authors: Kumar S, Gupta E, Kaushik S, Srivastava VK, Saxena J, Mehta S, Jyoti A Tags: Clin Chim Acta Source Type: research

Oncoproteomics: Current status and future opportunities.
Abstract Oncoproteomics is the systematic study of cancer samples using omics technologies to detect changes implicated in tumorigenesis. Recent progress in oncoproteomics is already opening new avenues for the identification of novel biomarkers for early clinical stage cancer detection, targeted molecular therapies, disease monitoring, and drug development. Such information will lead to new understandings of cancer biology and impact dramatically on the future care of cancer patients. In this review, we will summarize the advantages and limitations of the key technologies used in (onco)proteogenomics, (the Omics ...
Source: International Journal of Clinical Chemistry - June 6, 2019 Category: Chemistry Authors: He Y, Mohamedali A, Huang C, Baker MS, Nice EC Tags: Clin Chim Acta Source Type: research

A novel low-cost approach for the semi-quantitative analysis of carbohydrate-deficient transferrin (CDT) based on fluorescence resonance energy transfer (FRET).
Abstract BACKGROUND AND AIM: The increase of the carbohydrate-deficient transferrin (CDT) as results of an heavy intake of alcohol for at least two weeks, is a well-known biochemical modification since the middle '70s. Notwithstanding the first commercial kit for the diagnosis of chronic alcohol abuse based on this biomarker was commercially accessible already thirty years ago, only expensive analytical methods are currently available for its determination. The present paper shows a new approach intrinsically sensitive and specific, based on a specific derivatization of transferrin, and not requiring sophisticated...
Source: International Journal of Clinical Chemistry - June 6, 2019 Category: Chemistry Authors: Musile G, De Palo EF, Savchuk SA, Shestakova K, Bortolotti F, Tagliaro F Tags: Clin Chim Acta Source Type: research

Urinary neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury in sepsis patients in the emergency department.
Abstract BACKGROUND: Plasma neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for predicting acute kidney injury (AKI). The purpose of this study was to evaluate the diagnostic performance of urinary NGAL in predicting AKI in sepsis patients in the emergency department. METHODS: A total of 140 patients were enrolled. We compared serum procalcitonin and urinary NGAL concentrations between patients with local infection, sepsis, and septic shock, and between patients who did and did not develop AKI with sepsis. Receiver-operating characteristic curve analysis was used to evaluate the abilit...
Source: International Journal of Clinical Chemistry - June 5, 2019 Category: Chemistry Authors: Park HS, Kim JW, Lee KR, Hong DY, Park SO, Kim SY, Kim JY, Han SK Tags: Clin Chim Acta Source Type: research

Donor-specific circulating cell free DNA as a noninvasive biomarker of graft injury in heart transplantation.
CONCLUSION: These results suggest that cfDNA monitoring of transplanted patients may be a useful tool to detect and probably anticipate graft rejection. PMID: 31175849 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - June 5, 2019 Category: Chemistry Authors: Macher HC, García-Fernández N, Adsuar-Gómez A, Porras-López M, González-Calle A, Noval-Padillo J, Guerrero JM, Molinero P, Borrego-Domínguez JM, Herruzo-Avilés Á, Rubio A Tags: Clin Chim Acta Source Type: research

Application of contrast-enhanced ultrasound for scar pregnancy cases misdiagnosed as other diseases.
CONCLUSION: CEUS can be used as a supplementary method to diagnose scar pregnancy and evaluate the treatment efficacy, especially in cases with ambiguous results by TVS. PMID: 31173731 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - June 4, 2019 Category: Chemistry Authors: Liu D, Gu X, Liu F, Shi F, Yang M, Wu Q Tags: Clin Chim Acta Source Type: research

Evaluation of analytical factors associated with targeted MEFV gene sequencing using long-range PCR/massively parallel sequencing of whole blood DNA for molecular diagnosis of Familial Mediterranean fever.
CONCLUSIONS: Various analytical factors were evaluated, and the pipeline of targeted gene sequencing using LR-PCR and MPS was established. These data can enable the optimization of targeted gene sequencing using LR-PCR and MPS in the clinical laboratory. PMID: 31173732 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - June 4, 2019 Category: Chemistry Authors: Ishige T, Itoga S, Kawasaki K, Utsuno E, Beppu M, Sawai S, Nishimura M, Ichikawa T, Nomura F, Matsushita K Tags: Clin Chim Acta Source Type: research

Comprehensive immune complexome analysis detects disease-specific immune complex antigens in seminal plasma and follicular fluids derived from infertile men and women.
CONCLUSION: Functional characteristics of disease-specific antigens were found to correspond to the pathogenesis of male and female infertility. The formation of ICs may contribute to spermatogenic dysfunction and endometriosis-related infertility via loss of function of the related proteins. Immune complexome analysis is expected to be a valuable tool for the investigation of novel diagnostic methods and treatment strategies for infertility. PMID: 31158356 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 31, 2019 Category: Chemistry Authors: Murakami N, Kitajima M, Ohyama K, Aibara N, Taniguchi K, Wei M, Kitajima Y, Miura K, Masuzaki H Tags: Clin Chim Acta Source Type: research

What information can be obtained from the tears of a patient with primary open angle glaucoma?
Abstract Since tears are a biological fluid, they have a potential diagnostic value for ophthalmic diseases. The aim of this study was to compare tear supernatants and pellets obtained from patients suffering from primary open angle glaucoma (POAG) and healthy persons (HPs) using transmission electron microscopy (TEM) and molecular biological methods. Tear supernatants and pellets were prepared using ultrafiltration and ultracentrifugation and were examined by negative staining and immunogold labelling TEM. DNA of the pellets was isolated, quantified and sequenced using a MiSeq (Illumina, USA) genomic sequencer wi...
Source: International Journal of Clinical Chemistry - May 30, 2019 Category: Chemistry Authors: Tamkovich S, Grigor'eva A, Eremina A, Tupikin A, Kabilov M, Chernykh V, Vlassov V, Ryabchikova E Tags: Clin Chim Acta Source Type: research

Association of circulating growth differentiation factor-15, Kr üppel-like factor 4 and growth arrest-specific 6 with coronary artery disease.
Association of circulating growth differentiation factor-15, Krüppel-like factor 4 and growth arrest-specific 6 with coronary artery disease. Clin Chim Acta. 2019 May 30;: Authors: Liu H, Lyu YN, Li D, Cui Y, Dai W, Li Y Abstract BACKGROUND: Current assessment tools for patients with acute chest pain are either traumatic (coronary angiography) or unreliable (measurement of cardiac troponin concentrations). We investigated whether the novel cardiovascular stress markers, serum growth differentiation factor-15 (GDF-15), Krüppel-like factor 4 (KLF4) and growth arrest-specific 6 (gas6) may be us...
Source: International Journal of Clinical Chemistry - May 30, 2019 Category: Chemistry Authors: Liu H, Lyu YN, Li D, Cui Y, Dai W, Li Y Tags: Clin Chim Acta Source Type: research

Analytical performance evaluation of the Elecsys ® Troponin T Gen 5 STAT assay.
CONCLUSION: The TnT Gen 5 STAT assay demonstrated a CV of
Source: International Journal of Clinical Chemistry - May 29, 2019 Category: Chemistry Authors: Fitzgerald RL, Hollander JE, Peacock WF, Limkakeng AT, Breitenbeck N, Blechschmidt K, Laimighofer M, deFilippi C Tags: Clin Chim Acta Source Type: research

False decrease of high-sensitivity cardiac troponin T assay in pneumatic tube system samples.
Abstract BACKGROUND: The pneumatic tube system (PTS) is widely established in clinical laboratories. We aimed to evaluate the impacts of PTS on high-sensitivity cardiac troponin T (hs-cTnT) assays. METHODS: The hemolysis distribution of hs-cTnT PTS specimens from emergency department (ED) were determined by hemolysis index (HI). Grouped samples from 15 healthy volunteers were delivered to the laboratory via manual delivery (MD) or PTS. Interference studies were conducted to access the influence of different hemolysis degrees on hs-cTnT assays. RESULTS: 7.26% PTS specimens from ED were hemolyzed in clinic...
Source: International Journal of Clinical Chemistry - May 29, 2019 Category: Chemistry Authors: Wei J, Wu YN, Ling Y, Chen XT, Zhu Q, Xu J Tags: Clin Chim Acta Source Type: research

Evaluation of Martin's equation for LDL-C estimation in type 2 diabetes mellitus Egyptian patients.
CONCLUSION: The ME improves concordance of calculated LDL-C with a direct LDL-C assay in Egyptian diabetic patients. PMID: 31145894 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 27, 2019 Category: Chemistry Authors: Esawy MM, Shabana MA, Magdy MM Tags: Clin Chim Acta Source Type: research

Key questions about the future of laboratory medicine in the next decade of the 21st century.
Abstract This review advances the discussion about the future of Laboratory Medicine in the 2020s. In five major topic areas: 1. the "big picture" of healthcare; 2. pre-analytical factors; 3. Analytical factors; 4. post-analytical factors; and 5. relationships, which explores a next decade perspective on Laboratory Medicine and the likely impact of the predicted changes by means of a number of carefully focused questions that draw upon predictions made since 2013. The "big picture" of healthcare explores the effects of changing patient populations, the brain-to-brain loop, direct access testing...
Source: International Journal of Clinical Chemistry - May 27, 2019 Category: Chemistry Authors: Greaves R, Bernardini S, Ferrari M, Fortina P, Gouget B, Gruson D, Lang T, Loh TP, Morris H, Park JY, Roessler M, Yin P, Kricka LJ Tags: Clin Chim Acta Source Type: research

CXC chemokine ligand 12 (CXCL12) in atherosclerosis: An underlying therapeutic target.
Abstract CXC chemokine ligand 12 (CXCL12) is a specific chemokine ligand and plays a significant role in cell chemotaxis. Upon binding to CXC chemokine receptor 4 (CXCR4) or CXCR7, CXCL12 can activate different signaling cascades to regulate cell proliferation, migration, and metabolism. CXCL12 exerts a pro-atherogenic action by aggravating multiple pathogenesis of atherogenesis, including dyslipidemia, inflammation, neointima hyperplasia, angiogenesis, and insulin resistance. Serum CXCL12 levels are also markedly increased in patients with atherosclerosis-associated disease. The present review focuses on recent a...
Source: International Journal of Clinical Chemistry - May 27, 2019 Category: Chemistry Authors: Gao JH, Yu XH, Tang CK Tags: Clin Chim Acta Source Type: research

Liquid chromatography tandem mass spectrometry for plasma metadrenalines.
Abstract Evidence is accumulating which may result in plasma free metadrenalines (PMets) becoming the preferred test for diagnosing phaeochromocytoma and paraganglioma. Moreover, increased availability and benefits over other analytical methods like liquid chromatography with electrochemical detection and immunoassay are causing liquid chromatography tandem mass spectrometry (LC-MS/MS) to become the method of choice for PMet measurement. This review explores the evidence-base supporting this, and summarises published LC-MS/MS analytical methods for PMet analysis. Key aspects of methods (including SPE extraction, H...
Source: International Journal of Clinical Chemistry - May 25, 2019 Category: Chemistry Authors: Davies SL, Davison AS Tags: Clin Chim Acta Source Type: research

ST2 and the ST2/IL-33 signalling pathway-biochemistry and pathophysiology in animal models and humans.
Abstract ST2 is an interleukin (IL)-1 receptor family member with transmembrane (ST2L) and soluble (sST2) isoforms. Structurally, the ST2 gene products are very similar in mice and humans. In humans and in mice, alternative promoter activation and splicing produce ST2L and sST2. ST2L represents the longest transcript, whereas sST2 is the truncated, soluble isoform. ST2L is the biological receptor for IL-33, a member of the IL-1 family. IL-33 is the functional ligand of ST2L and signals the presence of tissue damage to local immune cells. IL-33/ST2L signalling leads to the production of inflammatory cytokines/chemo...
Source: International Journal of Clinical Chemistry - May 25, 2019 Category: Chemistry Authors: Pusceddu I, Dieplinger B, Mueller T Tags: Clin Chim Acta Source Type: research

Inherited monogenic defects of ceramide metabolism: Molecular bases and diagnoses.
Abstract Ceramides are membrane lipids implicated in the regulation of numerous biological functions. Recent evidence suggests that specific subsets of molecular species of ceramide may play distinct physiological roles. The importance of this family of molecules in vertebrates is witnessed by the deleterious consequences of genetic alterations in ceramide metabolism. This brief review summarizes the clinical presentation of human disorders due to the deficiency of enzymes involved either in the biosynthesis or the degradation of ceramides. Information on the possible underlying pathophysiological mechanisms is al...
Source: International Journal of Clinical Chemistry - May 22, 2019 Category: Chemistry Authors: Dubot P, Sabourdy F, Rybova J, Medin JA, Levade T Tags: Clin Chim Acta Source Type: research

Plasma EGFR mutation testing in non-small cell lung cancer: A value proposition.
Abstract Genomics-driven precision medicine using targeted therapies requires advanced molecular diagnostic tests. Decisions about the use and reimbursement for such tests are increasingly being made on the basis of more outcome-based and value-based approaches. The value proposition concept is a tool to assess the benefits of laboratory testing to each stakeholder of the care pathway with respect to outcomes. This concept was applied to the use of noninvasive plasma epidermal growth factor receptor (EGFR) mutation testing in patients with advanced or metastatic non-small cell lung cancer (NSCLC) to guide treatmen...
Source: International Journal of Clinical Chemistry - May 21, 2019 Category: Chemistry Authors: Oellerich M, Christenson RH, Beck J, Walson PD Tags: Clin Chim Acta Source Type: research

Clinical and molecular characteristics of carnitine-acylcarnitine translocase deficiency: Experience with six patients in Guangdong China.
This report describes the clinical characteristics, biochemical findings and molecular analysis of SLC25A20 gene of patients with CACTD in Guangdong. And our results show that the c.199-10T>G is likely the most common variant of CACTD in Guangdong population as it accounts for 83% (10/12) of the observed mutant alleles. Individuals with the c.199-10T>G genotype had a severe CACTD phenotype. PMID: 31108048 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 17, 2019 Category: Chemistry Authors: Tang C, Liu S, Wu M, Lin S, Lin Y, Su L, Zhang J, Feng Y, Huang Y Tags: Clin Chim Acta Source Type: research

Biological variation data for lipid cardiovascular risk assessment biomarkers. A systematic review applying the biological variation data critical appraisal checklist (BIVAC).
CONCLUSIONS: Application of BIVAC identifies BV data suitable for many important applications including setting APS. Additionally, this review identifies a need for new BIVAC compliant studies to deliver BV reference data in different subpopulations. PMID: 31103621 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 16, 2019 Category: Chemistry Authors: Marco JD, Fernández-Calle P, Minchinela J, Aarsand AK, Bartlett WA, Aslan B, Boned B, Braga F, Carobene A, Coskun A, Gonzalez-Lao E, Jonker N, Marques-Garcia F, Perich C, Ricos C, Simón M, Sandberg S Tags: Clin Chim Acta Source Type: research

Metabolomics workflow for lung cancer: Discovery of biomarkers.
Abstract Lung cancer is one of the most common cancers in the world. Due to the limitations of current diagnostic techniques and methods, most lung cancers are diagnosed at the advanced stage, which is not conducive to early treatment. The rise of metabolomics has provided new ideas for the early diagnosis of lung cancer. As a method for the comprehensive analysis of endogenous metabolites of the biological system, metabolomics has shown significant application potential for the early diagnosis and individualized treatment of various cancers including lung cancers. Via advanced analytical techniques and bioinforma...
Source: International Journal of Clinical Chemistry - May 16, 2019 Category: Chemistry Authors: Tang Y, Li Z, Lazar L, Fang Z, Tang C, Zhao J Tags: Clin Chim Acta Source Type: research

Substance P in cardiovascular diseases - A bioanalytical review.
Abstract Substance P has recently received much attention as a mediator of adverse heart remodelling and cardiac inflammation by releasing proinflammatory cytokines and matrix metalloproteases from immune and cardiac mast cells. Based on animal models, Substance P is highly associated with the development of cardiomyopathies, subsequently leading to heart failure. After a brief overview of the pathological role of Substance P in cardiac remodelling and cardiac inflammation, this review summarizes the limited, existing data of Substance P blood levels in adults with cardiovascular diseases, demonstrating a high var...
Source: International Journal of Clinical Chemistry - May 16, 2019 Category: Chemistry Authors: Feickert M, Burckhardt BB Tags: Clin Chim Acta Source Type: research

Serum lipoprotein-associated phospholipase A2 as a promising prognostic biomarker in association with 90-day outcome of acute intracerebral hemorrhage.
Abstract BACKGROUND: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is reflective of vascular inflammation and plays a role in the pathophysiology of cerebrovascular disease. We determine usefulness of serum Lp-PLA2 as a prognostic biomarker for intracerebral hemorrhage (ICH). METHODS: In this prospective, observational study, serum Lp-PLA2 concentrations were detected among 164 patients with acute spontaneous basal ganglia hemorrhage and 164 healthy controls. Using multivariate analysis, we analyzed its association with poor outcome (modified Rankin Scale>2) at poststroke 90 days and hemorrhagic seve...
Source: International Journal of Clinical Chemistry - May 16, 2019 Category: Chemistry Authors: Bian L, Mao LG, Sun Y, Shen F, Chen JF, Liu Z, Zhou W Tags: Clin Chim Acta Source Type: research

Advances in the relationship between glycosyltransferases and multidrug resistance in cancer.
Abstract Despite great progress in clinical treatment, cancer remains a serious health problem contributing to significant morbidity and mortality worldwide. Although chemotherapy is a common therapeutic measure, multidrug resistance (MDR) presents a major challenge that often leads to poor prognosis. The abnormal expression of glycosyltransferases (GTs) leading to aberrant glycosylation patterns are considered a marker of cancer. Furthermore, the biosynthesis of these glycoconjugates has been associated with tumor proliferation, invasion and metastasis. Recently, studies have found that GTs are involved in mediat...
Source: International Journal of Clinical Chemistry - May 15, 2019 Category: Chemistry Authors: Wu Y, Chen X, Wang S, Wang S Tags: Clin Chim Acta Source Type: research

Simultaneous quantification of 48 plasma amino acids by liquid chromatography-tandem mass spectrometry to investigate urea cycle disorders.
Abstract Urea cycle disorders (UCD) are inborn errors of ammonia detoxification in which early diagnosis and treatment are critical to prevent metabolic emergencies. Unfortunately, the diagnosis was often and pronounced delayed. To improve diagnosis, we developed herein a liquid chromatography-tandem mass spectrometry method to investigate the disturbance of amino acid profile caused by UCD. The method enabled absolute quantification of 48 amino acids (AAs) within 20 min. Only 2.5 μL plasma was required for the analysis. The lower limits of quantification for most AAs were 0.01 μmol/L. Method accuracie...
Source: International Journal of Clinical Chemistry - May 13, 2019 Category: Chemistry Authors: Peng MZ, Cai YN, Shao YX, Zhao L, Jiang MY, Lin YT, Yin X, Sheng HY, Liu L Tags: Clin Chim Acta Source Type: research

Comparison of non-fasting LDL-C levels calculated by Friedewald formula with those directly measured in Chinese patients with coronary heart disease after a daily breakfast.
Abstract BACKGROUND: LDL-C level can be measured by direct methods (LDL-CM) or calculated by Friedewald formula (LDL-CC). The aim of this study was to investigate the difference between LDL-CM and LDL-CC after a daily breakfast in Chinese patients with coronary heart disease (CHD). METHODS: Three hundred and three inpatients, including 203 CHD patients (CHD group) and 100 non-CHD controls (CON group), were enrolled in this study. Serum levels of blood lipid parameters, including LDL-CC and LDL-CM, at 0, 2 and 4 h (h) were monitored after a daily breakfast in all subjects. RESULTS: LDL-CM was significan...
Source: International Journal of Clinical Chemistry - May 11, 2019 Category: Chemistry Authors: Lin QZ, Chen YQ, Guo LL, Xiang QY, Tian F, Wen T, Liu L Tags: Clin Chim Acta Source Type: research

Serum albumin saturation test based on non-esterified fatty acids imbalance for clinical employment.
him M Abstract Fatty acids are fundamental as energy and structural source to the human cells. They are not usually found free in human circulation. Alteration in fatty acids metabolism is linked to diseases such as diabetes, preeclampsia, heart disease, and some infectious diseases. Increased levels of non-esterified fatty acids (NEFA) may cause cell dysfunction and lipotoxicity. Since physiologically fatty acids are transported bound to albumin, we propose here a simple and cheap test that consists of albumin isoelectric focusing determination to measure the potential systemic NEFA cytotoxicity. For validation o...
Source: International Journal of Clinical Chemistry - May 10, 2019 Category: Chemistry Authors: Gonçalves-de-Albuquerque CF, Barnese MRC, Soares MA, Faria MVC, Silva AR, Neto HCCF, Burth P, Younes-Ibrahim M Tags: Clin Chim Acta Source Type: research

The VLDL receptor plays a key role in the metabolism of postprandial remnant lipoproteins.
Abstract A new concept to account for the process of postprandial remnant lipoprotein metabolism is proposed based on the characteristics of lipoprotein particles and their receptors. The characteristics of remnant lipoprotein (RLP) were investigated using an immuno-separation method. The majority of the postprandial lipoproteins increased after fat intake was shown to be VLDL remnants, not chylomicron (CM) remnants, based on the significantly high ratio of apoB100/apoB48 in the RLP and the high degree of similarity in the particle size of the apoB48 and apoB100 carrying lipoproteins, which fluctuate in parallel d...
Source: International Journal of Clinical Chemistry - May 9, 2019 Category: Chemistry Authors: Nakajima K, Tokita Y, Tanaka A, Takahashi S Tags: Clin Chim Acta Source Type: research

Estimating the glomerular filtration rate and tubular dysfunction in an elderly population with normoalbuminuria in China.
Abstract BACKGROUND: The prevalence and clinical risk factors of normoalbuminuric renal impairment have not yet been investigated in elderly Chinese populations. To clarify this, we conducted survey research on an elderly Chinese community population. METHODS: A total of 691 elderly community participants were included in this study. Normoalbuminuria was defined as a urinary albumin to creatinine ratio (ACR) 60 and 
Source: International Journal of Clinical Chemistry - May 9, 2019 Category: Chemistry Authors: Wang H, Lou Y, Ma Y, Shan X Tags: Clin Chim Acta Source Type: research

Soluble neural cell adhesion molecule and behavioural recovery in minimally conscious patients undergoing transcranial direct current stimulation.
CONCLUSIONS: This exploratory research suggests the sNCAM levels, potentially associated with tDCS outcomes, as a candidate biomarker of neurobiological after-effects in MCS patients. PMID: 31077650 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 8, 2019 Category: Chemistry Authors: Ziliotto N, Marchetti G, Straudi S, Tisato V, Lavezzi S, Manfredini F, Basaglia N, Bernardi F Tags: Clin Chim Acta Source Type: research

Comparison of liquid chromatography with tandem mass spectrometry and ion-exchange chromatography by post-column ninhydrin derivatization for amino acid monitoring.
Abstract OBJECTIVES: Precise quantification of amino acids (AAs) is mandatory for successful diagnosis and monitoring of patients with metabolic diseases. We compared ion-exchange chromatography (IEC) and liquid chromatography with tandem mass spectrometry (LC-MS/MS), the two methods most commonly used in clinical laboratories for the quantification of AAs in physiological samples. DESIGN & METHODS: 123 apparently healthy children were selected for the study. The plasma samples for LC-MS/MS were prepared accordingly to the aTRAQ Kit for Physiological Fluids on Sciex 3200 Qtrap, for IEC according to the pr...
Source: International Journal of Clinical Chemistry - May 8, 2019 Category: Chemistry Authors: Smon A, Cuk V, Brecelj J, Murko S, Groselj U, Tansek MZ, Battelino T, Lampret BR Tags: Clin Chim Acta Source Type: research

PCSK9: A new participant in lipophagy in regulating atherosclerosis?
Abstract Proprotein convertase subtilisin kexin 9 (PCSK9) regulates lipid metabolism by degrading low-density lipoprotein receptor on the surface of hepatocytes. PCSK9-mediated lipid degradation is associated with lipophagy. Lipophagy is a process by which autophagosomes selectively sequester lipid-droplet-stored lipids and are delivered to lysosomes for degradation. Lipophagy was first discovered in hepatocytes, and its occurrence provides important fundamental insights into how lipid metabolism regulates cellular physiology and pathophysiology. Furthermore, PCSK9 may regulate lipid levels by affecting lipophagy....
Source: International Journal of Clinical Chemistry - May 7, 2019 Category: Chemistry Authors: Xiao J, Deng YM, Liu XR, Cao JP, Zhou M, Tang YL, Xiong WH, Jiang ZS, Tang ZH, Liu LS Tags: Clin Chim Acta Source Type: research

Association of IL-10 to coronary disease severity in patients with metabolic syndrome.
Abstract BACKGROUND: Metabolic syndrome (MetS) is a group of risk factors that increase the risk for heart disease. Little is known about the role of IL-10 in the severity of coronary artery disease (CAD) in patients with MetS. We investigated plasma levels of IL-10 and other pro-inflammatory cytokines in patients with MetS with or without severe CAD. METHODS: Cross-sectional study with healthy and MetS individuals. IL-10 and other pro-inflammatory interleukins were analyzed in 90 subjects divided into 3 groups: group 1 (n = 30), patients with MetS without severe CAD; group 2 (n = 30), patients with M...
Source: International Journal of Clinical Chemistry - May 7, 2019 Category: Chemistry Authors: Barcelos ALV, de Oliveira EA, Haute GV, Costa BP, Pedrazza L, Donadio MVF, de Oliveira JR, Bodanese LC Tags: Clin Chim Acta Source Type: research

Corrigendum to "Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR" [Clinica Chimica Acta 491 (2019) 91-96].
Corrigendum to "Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR" [Clinica Chimica Acta 491 (2019) 91-96]. Clin Chim Acta. 2019 May 06;: Authors: Xu X, Ma X, Zhang X, Cao G, Tang Y, Deng X, Kang Z, Li M, Guan M PMID: 31072595 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 6, 2019 Category: Chemistry Authors: Xu X, Ma X, Zhang X, Cao G, Tang Y, Deng X, Kang Z, Li M, Guan M Tags: Clin Chim Acta Source Type: research

Quantitative proteomics of changes in succinylated proteins expression profiling in left appendages tissue from valvular heart disease patients with atrial fibrillation.
Abstract BACKGROUND: Previous studies have suggested that proteomic modifications are closely associated with cardiovascular diseases. The aim of this study was to identify potential mechanisms by profiling the changes in succinylated protein expression in left appendage tissues from patients with valvular heart disease and atrial fibrillation (AF). METHODS: Using dimethyl labeling for relative and absolute quantification-coupled high-performance liquid chromatography-tandem mass spectrometry, we analyzed the proteomics profiles and succinylation events in 18 left atrial appendage tissue samples from patients...
Source: International Journal of Clinical Chemistry - May 3, 2019 Category: Chemistry Authors: Bai F, Tu T, Qin F, Ma Y, Liu N, Liu Y, Liao X, Zhou S, Liu Q Tags: Clin Chim Acta Source Type: research

Increased plasma sestrin2 concentrations in patients with chronic heat failure and predicted the occurrence of major adverse cardiac events: A 36-month follow-up cohort study.
CONCLUSIONS: Plasma Sesn2 concentrations were increased in CHF patients and positively correlated with the severity of CHF. Increased Sesn2 concentrations significantly increased the occurrence of major adverse cardiac events and suggested poor outcome in CHF patients. PMID: 31059702 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 3, 2019 Category: Chemistry Authors: Wang H, Li N, Shao X, Li J, Guo L, Yu X, Sun Y, Hao J, Niu H, Xiang J, Li X, Han X Tags: Clin Chim Acta Source Type: research

Study on urine metabolic profiling and pathogenesis of hyperlipidemia.
CONCLUSION: The non-targeted metabolomic method based on UPLC-Q-TOF/MS technology can effectively identify potential biomarkers in the urine of HLP patients and explore the possible pathogenesis. Our research will lay a foundation for finding new targets for the treatment of HLP and provide a basis for clinical research on the treatment of HLP. PMID: 31059703 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 3, 2019 Category: Chemistry Authors: Yang L, Li Z, Song Y, Liu Y, Zhao H, Liu Y, Zhang T, Yuan Y, Cai X, Wang S, Wang P, Gao S, Li L, Li Y, Yu C Tags: Clin Chim Acta Source Type: research

Liquid biopsy: Circulating exosomal long noncoding RNAs in cancer.
Abstract Despite many advances in diagnostics and multimodal treatment (surgery, radiotherapy, chemotherapy), cancer still remains one of the most important public health challenges worldwide because of the associated morbidity and mortality. Liquid biopsy has been developed to detect cancer at an early stage based on minimally invasive and serial body fluid tests with the advantage of following tumor evolution in real time. Circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), circulating cell-free noncoding RNAs (cfRNAs) and circulating exosomes represent the major components of liquid biopsy analysis. ...
Source: International Journal of Clinical Chemistry - May 2, 2019 Category: Chemistry Authors: Jiang N, Pan J, Fang S, Zhou C, Han Y, Chen J, Meng X, Jin X, Gong Z Tags: Clin Chim Acta Source Type: research

A post-hoc subgroup analysis assessing acute cardiac biomarker profiles in female cancer patients during adjuvant therapy.
PMID: 31054914 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - May 2, 2019 Category: Chemistry Authors: Mackett K, Dhesy-Thind S, Donovan EK, Mukherjee S, Swaminath A, Leong DP, Voruganti S, Sussman J, Wright J, Okawara G, Fraser G, Sagar S, Bordeleau L, Ellis PM, Hirte H, Kavsak PA Tags: Clin Chim Acta Source Type: research

Parkinson's and Lewy body dementia CSF biomarkers.
Abstract The clinical diagnosis of Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) is challenging due to highly variable clinical presentation and clinical and pathological overlap with other neurodegenerative diseases. Since cerebrospinal fluid (CSF) mirrors the pathological changes taking place in the brain, it represents a promising source of biomarkers. With respect to classical AD biomarkers, low CSF Aβ42 levels have shown a robust prognostic value in terms of development of cognitive impairment in PD and DLB. In the differential diagnosis between AD and DLB, a potential role of t-tau, p-tau...
Source: International Journal of Clinical Chemistry - April 30, 2019 Category: Chemistry Authors: Parnetti L, Paciotti S, Farotti L, Bellomo G, Sepe FN, Eusebi P Tags: Clin Chim Acta Source Type: research

Diagnosis associated with Tau higher than 1200  pg/mL: Insights from the clinical and laboratory practice.
Diagnosis associated with Tau higher than 1200 pg/mL: Insights from the clinical and laboratory practice. Clin Chim Acta. 2019 Apr 30;: Authors: Lehmann S, Paquet C, Malaplate-Armand C, Magnin E, Schraen S, Quillard-Muraine M, Bousiges O, Delaby C, Dumurgier J, Hugon J, Sablonnière B, Blanc F, Wallon D, Gabelle A, Laplanche JL, Bouaziz-Amar E, Peoc'h K, ePLM Group Abstract CONTEXT: Cerebrospinal fluid (CSF) biomarkers are valuable tools for the diagnosis of neurological diseases. We aimed to investigate within a retrospective multicentric study the final diagnosis associated with very high CS...
Source: International Journal of Clinical Chemistry - April 30, 2019 Category: Chemistry Authors: Lehmann S, Paquet C, Malaplate-Armand C, Magnin E, Schraen S, Quillard-Muraine M, Bousiges O, Delaby C, Dumurgier J, Hugon J, Sablonnière B, Blanc F, Wallon D, Gabelle A, Laplanche JL, Bouaziz-Amar E, Peoc'h K, ePLM Group Tags: Clin Chim Acta Source Type: research

BCL10 in cell survival after DNA damage.
This article reviews the role of BCL10 in cell survival following DNA damage. PMID: 31047877 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 29, 2019 Category: Chemistry Authors: Luo Y, Wu J, Zou J, Cao Y, He Y, Ling H, Zeng T Tags: Clin Chim Acta Source Type: research

Serum netrin-1 serves as a prognostic biomarker of aneurysmal subarachnoid hemorrhage.
This study was designed to discern the utility of serum netrin-1 as a biomarker for assessing the severity and prognosis of patients with aneurysmal SAH. METHODS: Netrin-1 concentrations were gauged in serum from 104 patients and 104 controls. Hemorrhagic clinical and radiological severity was assessed utilizing World Federation of Neurological Surgeons (WFNS) score, modified Fisher score, and Hunt Hess score. Glasgow Outcome Scale (GOS) score was recorded at 6 months after SAH. GOS score of 1-3 was considered as a poor outcome. RESULTS: Patients showed substantially lower serum netrin-1 concentrations than con...
Source: International Journal of Clinical Chemistry - April 29, 2019 Category: Chemistry Authors: Chen JL, Yuan DH, Yang SJ, Gu C, Zhou HS, Shao GF Tags: Clin Chim Acta Source Type: research

Measurement of calcineurin activity in peripheral blood mononuclear cells by ultra-high performance liquid chromatography-tandem mass spectrometry. Renal transplant recipients application (pharmacodynamic monitoring).
Lloberas N Abstract BACKGROUND: Therapeutic drug monitoring of calcineurin inhibitor, tacrolimus (TAC), is routinely used in post-transplantation. Currently, measurement of calcineurin activity has been proposed as a promising clinical tool to evaluate efficacy and to optimize drug dosing. The main aim of our study was to develop a method to measure phosphatase calcineurin activity (CNA) in peripheral blood mononuclear cells (PBMCs) by ultra-high performance liquid chromatography-tandem mass spectrometry and to validate it following FDA and EMA guidelines. METHODS: This methodology is based on monitoring the...
Source: International Journal of Clinical Chemistry - April 27, 2019 Category: Chemistry Authors: Fontova P, Rigo-Bonnin R, Vidal-Alabró A, Cerezo G, Bestard O, Cruzado JM, Torras J, Grinyó JM, Lloberas N Tags: Clin Chim Acta Source Type: research

The potentiality of salivary peptide biomarkers for screening patients with periodontal diseases by mass spectrometry.
CONCLUSIONS: Some peptides in saliva, GCF, and serum act as biomarkers for chronic periodontitis and gingivitis, which have certain potentiality for screening patients with periodontal diseases and distinguishing them from healthy individuals in a comparatively large population by mass spectrometry. PMID: 31026423 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 23, 2019 Category: Chemistry Authors: Tang H, Yuan C, Ma Z, Zhu C, Tong P, Gallagher JE, Sun X, Zheng S Tags: Clin Chim Acta Source Type: research

Applying a multiplexed primer extension method on dried blood spots increased the detection of carriers at risk of glucose-6-phosphate dehydrogenase deficiency in newborn screening program.
CONCLUSIONS: Our approach could significantly increase the detection rate of male patients and female carriers with a reasonable cost and a reasonable number of clinic referrals. PMID: 31022393 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - April 22, 2019 Category: Chemistry Authors: Chiu YH, Chen HJ, Chang YC, Liu YN, Kao SM, Liu MY, Weng YY, Hsiao KJ, Liu TT Tags: Clin Chim Acta Source Type: research