Pregnancy-specific reference intervals for C-reactive protein improve diagnostic accuracy for infection: a longitudinal study
Clin Chim Acta. 2021 Feb 26:S0009-8981(21)00063-2. doi: 10.1016/j.cca.2021.02.015. Online ahead of print.ABSTRACTBACKGROUND AND AIMS: Infections are a major cause of maternal mortality. C-reactive protein (CRP), a commonly-used inflammatory marker, is widely used to inform diagnosis, but the upper limit of normal in pregnancy is uncertain. We have defined trimester-specific reference intervals for CRP and evaluated their diagnostic accuracy for infection.MATERIALS AND METHODS: Development cohort: longitudinal study of pregnant women to determine 95% reference intervals. Evaluation cohort: diagnostic accuracy study to evalu...
Source: International Journal of Clinical Chemistry - March 1, 2021 Category: Chemistry Authors: Samuel Dockree Jennifer Brook Tim James Brian Shine Lawrence Impey Manu Vatish Source Type: research

Pregnancy-specific reference intervals for C-reactive protein improve diagnostic accuracy for infection: a longitudinal study
Clin Chim Acta. 2021 Feb 26:S0009-8981(21)00063-2. doi: 10.1016/j.cca.2021.02.015. Online ahead of print.ABSTRACTBACKGROUND AND AIMS: Infections are a major cause of maternal mortality. C-reactive protein (CRP), a commonly-used inflammatory marker, is widely used to inform diagnosis, but the upper limit of normal in pregnancy is uncertain. We have defined trimester-specific reference intervals for CRP and evaluated their diagnostic accuracy for infection.MATERIALS AND METHODS: Development cohort: longitudinal study of pregnant women to determine 95% reference intervals. Evaluation cohort: diagnostic accuracy study to evalu...
Source: International Journal of Clinical Chemistry - March 1, 2021 Category: Chemistry Authors: Samuel Dockree Jennifer Brook Tim James Brian Shine Lawrence Impey Manu Vatish Source Type: research

Lipid metabolism changes in patients with severe COVID-19
Clin Chim Acta. 2021 Feb 24:S0009-8981(21)00052-8. doi: 10.1016/j.cca.2021.02.011. Online ahead of print.ABSTRACTBACKGROUND: We investigated the dynamic changes in lipid profiles and their correlations with disease severity and clinical outcome in patients with severe COVID-19.METHODS: We retrospectively reviewed 519 severe COVID-19 patients with confirmed outcomes (discharged or deceased), admitted to the West Court of Union Hospital in Wuhan, China, between 29 January and 8 April 2020.RESULTS: Altogether, 424 severe COVID-19 patients, including 34 non-survivors and 390 survivors, were included in the final analyses. Duri...
Source: International Journal of Clinical Chemistry - February 27, 2021 Category: Chemistry Authors: Yi Li Yan Zhang Rongli Lu Minhui Dai Minxue Shen Jianchu Zhang Yanhui Cui Ben Liu Fengyu Lin Lingli Chen Duoduo Han Yifei Fan Yanjun Zeng Wen Li Sha Li Xiang Chen Haitao Li Pinhua Pan Source Type: research

Lipid metabolism changes in patients with severe COVID-19
Clin Chim Acta. 2021 Feb 24:S0009-8981(21)00052-8. doi: 10.1016/j.cca.2021.02.011. Online ahead of print.ABSTRACTBACKGROUND: We investigated the dynamic changes in lipid profiles and their correlations with disease severity and clinical outcome in patients with severe COVID-19.METHODS: We retrospectively reviewed 519 severe COVID-19 patients with confirmed outcomes (discharged or deceased), admitted to the West Court of Union Hospital in Wuhan, China, between 29 January and 8 April 2020.RESULTS: Altogether, 424 severe COVID-19 patients, including 34 non-survivors and 390 survivors, were included in the final analyses. Duri...
Source: International Journal of Clinical Chemistry - February 27, 2021 Category: Chemistry Authors: Yi Li Yan Zhang Rongli Lu Minhui Dai Minxue Shen Jianchu Zhang Yanhui Cui Ben Liu Fengyu Lin Lingli Chen Duoduo Han Yifei Fan Yanjun Zeng Wen Li Sha Li Xiang Chen Haitao Li Pinhua Pan Source Type: research

Chemerin in inflammatory diseases
Clin Chim Acta. 2021 Feb 22:S0009-8981(21)00051-6. doi: 10.1016/j.cca.2021.02.010. Online ahead of print.ABSTRACTObesity is associated with a series of health problems. Adipocytes are a huge repository of energy as well as an important source of many adipokines. In obesity, adipocytes are dysfunctional with excessive production and secretion of pro-inflammatory adipokines, such as tumor necrosis factor α (TNF-α), leptin, and chemerin. Recent studies have revealed that chemerin plays an important role in modulating physiologic as well as pathophysiologic processes. For example, chemerin stimulates maturation and...
Source: International Journal of Clinical Chemistry - February 25, 2021 Category: Chemistry Authors: Xin Su Ye Cheng Guoming Zhang Bin Wang Source Type: research

Correlation of Krebs von den Lungen-6 and Fibronectin with pulmonary fibrosis in Coronavirus Disease 2019
CONCLUSION: Serum KL-6 concentration was significantly elevated in severe COVID-19 patients, which may be useful for evaluating the disease severity. For early prevention of the development of pulmonary fibrosis, high concentrations of serum KL-6 in the early stage of COVID-19 should be paid close attention.PMID:33631198 | PMC:PMC7898973 | DOI:10.1016/j.cca.2021.02.012 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 25, 2021 Category: Chemistry Authors: Ding-Hui Peng Yi Luo Li-Jun Huang Fan-Lu Liao Yan-Yuan Liu Peng Tang Han-Ning Hu Wei Chen Source Type: research

Chemerin in inflammatory diseases
Clin Chim Acta. 2021 Feb 22:S0009-8981(21)00051-6. doi: 10.1016/j.cca.2021.02.010. Online ahead of print.ABSTRACTObesity is associated with a series of health problems. Adipocytes are a huge repository of energy as well as an important source of many adipokines. In obesity, adipocytes are dysfunctional with excessive production and secretion of pro-inflammatory adipokines, such as tumor necrosis factor α (TNF-α), leptin, and chemerin. Recent studies have revealed that chemerin plays an important role in modulating physiologic as well as pathophysiologic processes. For example, chemerin stimulates maturation and...
Source: International Journal of Clinical Chemistry - February 25, 2021 Category: Chemistry Authors: Xin Su Ye Cheng Guoming Zhang Bin Wang Source Type: research

Correlation of Krebs von den Lungen-6 and Fibronectin with pulmonary fibrosis in Coronavirus Disease 2019
CONCLUSION: Serum KL-6 concentration was significantly elevated in severe COVID-19 patients, which may be useful for evaluating the disease severity. For early prevention of the development of pulmonary fibrosis, high concentrations of serum KL-6 in the early stage of COVID-19 should be paid close attention.PMID:33631198 | PMC:PMC7898973 | DOI:10.1016/j.cca.2021.02.012 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 25, 2021 Category: Chemistry Authors: Ding-Hui Peng Yi Luo Li-Jun Huang Fan-Lu Liao Yan-Yuan Liu Peng Tang Han-Ning Hu Wei Chen Source Type: research

Salivary SARS-CoV-2 antigen rapid detection: a prospective cohort study
CONCLUSIONS: Self-collected saliva is a valid alternative to NPS for SARS-CoV-2 detection by molecular, but also by CLEIA antigen testing, which is therefore potentially useful for large scale screening.PMID:33626369 | PMC:PMC7897404 | DOI:10.1016/j.cca.2021.02.014 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 24, 2021 Category: Chemistry Authors: Daniela Basso Ada Aita Andrea Padoan Chiara Cosma Filippo Navaglia Stefania Moz Nicole Contran Carlo-Federico Zambon Anna Maria Cattelan Mario Plebani Source Type: research

Salivary SARS-CoV-2 antigen rapid detection: a prospective cohort study
CONCLUSIONS: Self-collected saliva is a valid alternative to NPS for SARS-CoV-2 detection by molecular, but also by CLEIA antigen testing, which is therefore potentially useful for large scale screening.PMID:33626369 | PMC:PMC7897404 | DOI:10.1016/j.cca.2021.02.014 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 24, 2021 Category: Chemistry Authors: Daniela Basso Ada Aita Andrea Padoan Chiara Cosma Filippo Navaglia Stefania Moz Nicole Contran Carlo-Federico Zambon Anna Maria Cattelan Mario Plebani Source Type: research

The DNA replication regulator MCM6: An emerging cancer biomarker and target
Clin Chim Acta. 2021 Feb 17:S0009-8981(21)00046-2. doi: 10.1016/j.cca.2021.02.005. Online ahead of print.ABSTRACTMCM6 is a significant DNA replication regulator that plays a crucial role in sustaining the cell cycle. In many cancer cells, MCM6 expression is enhanced. For example, persistently increased expression of MCM6 promotes the formation, development and progression of hepatocellular carcinoma (HCC). Up- and down-regulation studies have indicated that MCM6 regulates cell cycle, proliferation, metastasis, immune response and the maintenance of the DNA replication system. MCM6 can also regulate downstream signaling suc...
Source: International Journal of Clinical Chemistry - February 20, 2021 Category: Chemistry Authors: Tian Zeng Yang Guan Yu-Kun Li Qing Wu Xiao-Jun Tang Xin Zeng Hui Ling Juan Zou Source Type: research

Extracellular vesicle YRNA in atherosclerosis
Clin Chim Acta. 2021 Feb 17:S0009-8981(21)00044-9. doi: 10.1016/j.cca.2021.02.003. Online ahead of print.ABSTRACTAtherosclerosis is characterized by lipid accumulation and chronic inflammation. The accumulation of apoptotic foam cells can induce the secretion of proinflammatory factors and necrosis of atherosclerotic plaque tissue. Numerous studies have demonstrated that extracellular vesicle (EV)-enclosed YRNAs and their fragments, YsRNAs, play important roles in atherosclerosis initiation, progression, and diagnosis. YsRNA-5p transcripts promote foam cell apoptosis and inflammatory responses by binding to Ro60 in vitro a...
Source: International Journal of Clinical Chemistry - February 20, 2021 Category: Chemistry Authors: Wujun Chen Lu Li Jie Wang Qian Li Renshuai Zhang Shuai Wang Yudong Wu Dongming Xing Source Type: research

The DNA replication regulator MCM6: An emerging cancer biomarker and target
Clin Chim Acta. 2021 Feb 17:S0009-8981(21)00046-2. doi: 10.1016/j.cca.2021.02.005. Online ahead of print.ABSTRACTMCM6 is a significant DNA replication regulator that plays a crucial role in sustaining the cell cycle. In many cancer cells, MCM6 expression is enhanced. For example, persistently increased expression of MCM6 promotes the formation, development and progression of hepatocellular carcinoma (HCC). Up- and down-regulation studies have indicated that MCM6 regulates cell cycle, proliferation, metastasis, immune response and the maintenance of the DNA replication system. MCM6 can also regulate downstream signaling suc...
Source: International Journal of Clinical Chemistry - February 20, 2021 Category: Chemistry Authors: Tian Zeng Yang Guan Yu-Kun Li Qing Wu Xiao-Jun Tang Xin Zeng Hui Ling Juan Zou Source Type: research

Extracellular vesicle YRNA in atherosclerosis
Clin Chim Acta. 2021 Feb 17:S0009-8981(21)00044-9. doi: 10.1016/j.cca.2021.02.003. Online ahead of print.ABSTRACTAtherosclerosis is characterized by lipid accumulation and chronic inflammation. The accumulation of apoptotic foam cells can induce the secretion of proinflammatory factors and necrosis of atherosclerotic plaque tissue. Numerous studies have demonstrated that extracellular vesicle (EV)-enclosed YRNAs and their fragments, YsRNAs, play important roles in atherosclerosis initiation, progression, and diagnosis. YsRNA-5p transcripts promote foam cell apoptosis and inflammatory responses by binding to Ro60 in vitro a...
Source: International Journal of Clinical Chemistry - February 20, 2021 Category: Chemistry Authors: Wujun Chen Lu Li Jie Wang Qian Li Renshuai Zhang Shuai Wang Yudong Wu Dongming Xing Source Type: research

DLX6-AS1 is a potential biomarker and therapeutic target in cancer initiation and progression
Clin Chim Acta. 2021 Feb 17;517:1-8. doi: 10.1016/j.cca.2021.02.006. Online ahead of print.ABSTRACTLong noncoding RNAs (lncRNAs) are involved in multiple functions such as the regulation of cellular homeostasis. They play prominent roles in the pathogenesis of human cancer, and contribute to every hallmark of cancer. The novel cancer-related lncRNA DLX6 antisense RNA 1 (DLX6-AS1) plays an essential regulatory role in enhancing and initiating carcinogenesis and tumor progression. This progression is due to the aberrant regulation of downstream factors in vitro as well as in vivo. DLX6-AS1 is significantly dysregulated in va...
Source: International Journal of Clinical Chemistry - February 19, 2021 Category: Chemistry Authors: Qiuxian Zheng Xinyu Gu Qin Yang Qingfei Chu Yiyang Dai Zhi Chen Source Type: research

Utility of serum NOX4 as a potential prognostic biomarker for aneurysmal subarachnoid hemorrhage
CONCLUSIONS: Serum NOX4 concentrations, in close correlation with hemorrhagic severity, were independently associated with DCI and poor clinical outcome after hemorrhagic stroke, substantializing serum NOX4 as a promising prognostic biomarker for aSAH.PMID:33607069 | DOI:10.1016/j.cca.2021.02.007 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 19, 2021 Category: Chemistry Authors: Jianwei Pan Lingdi Lao Jian Shen Sichong Huang Tiesong Zhang Weijian Fan Ming Yan Jun Gu Wenchao Liu Source Type: research

Identification of the first Alu-mediated gross deletion involving the BCKDHA gene in a compound heterozygous patient with maple syrup urine disease
CONCLUSIONS: This study is the first time report on rearrangement sequences in BCKDHA mediated by Alu element, which resulted in MSUD. Our results may also offer new insights into the formation and pathogenicity of MSUD, and may be useful to genetic counseling and genetic testing.PMID:33607070 | DOI:10.1016/j.cca.2021.01.023 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 19, 2021 Category: Chemistry Authors: Shujun Ma Zhongxin Zhang Yanyan Fu Mingxia Zhang Yuna Niu Ruiguang Li Qinghe Guo Zhian He Qingwei Zhao Zhishan Song Xia Wang Ruili Sun Source Type: research

Omentin-1: protective impact on ischemic stroke via ameliorating atherosclerosis
This study is the first to summarize the comprehensive mechanisms between omentin-1 and atherosclerosis and to review the shielding effect of omentin-1 on IS. We shed light on omentin-1 as a novel therapeutic target for combating IS.PMID:33607071 | DOI:10.1016/j.cca.2021.02.004 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 19, 2021 Category: Chemistry Authors: Shiyi Lin Xin Li Jiabei Zhang Yuyang Zhang Source Type: research

The DNA replication regulator MCM6: An emerging cancer biomarker and target.
Abstract MCM6 is a significant DNA replication regulator that plays a crucial role in sustaining the cell cycle. In many cancer cells, MCM6 expression is enhanced. For example, persistently increased expression of MCM6 promotes the formation, development and progression of hepatocellular carcinoma (HCC). Up- and down-regulation studies have indicated that MCM6 regulates cell cycle, proliferation, metastasis, immune response and the maintenance of the DNA replication system. MCM6 can also regulate downstream signaling such as MEK/ERK thus promoting carcinogenesis. Accordingly, MCM6 may represent a sensitive and spe...
Source: International Journal of Clinical Chemistry - February 17, 2021 Category: Chemistry Authors: Zeng T, Guan Y, Li YK, Wu Q, Tang XJ, Zeng X, Ling H, Zou J Tags: Clin Chim Acta Source Type: research

Extracellular vesicle YRNA in atherosclerosis.
Abstract Atherosclerosis is characterized by lipid accumulation and chronic inflammation. The accumulation of apoptotic foam cells can induce the secretion of proinflammatory factors and necrosis of atherosclerotic plaque tissue. Numerous studies have demonstrated that extracellular vesicle (EV)-enclosed YRNAs and their fragments, YsRNAs, play important roles in atherosclerosis initiation, progression, and diagnosis. YsRNA-5p transcripts promote foam cell apoptosis and inflammatory responses by binding to Ro60 in vitro and in vivo. YRNAs may regulate atherosclerosis progression by binding to several proteins, incl...
Source: International Journal of Clinical Chemistry - February 17, 2021 Category: Chemistry Authors: Chen W, Li L, Wang J, Li Q, Zhang R, Wang S, Wu Y, Xing D Tags: Clin Chim Acta Source Type: research

DLX6-AS1 is a potential biomarker and therapeutic target in cancer initiation and progression.
Abstract Long noncoding RNAs (lncRNAs) are involved in multiple functions such as the regulation of cellular homeostasis. They play prominent roles in the pathogenesis of human cancer, and contribute to every hallmark of cancer. The novel cancer-related lncRNA DLX6 antisense RNA 1 (DLX6-AS1) plays an essential regulatory role in enhancing and initiating carcinogenesis and tumor progression. This progression is due to the aberrant regulation of downstream factors in vitro as well as in vivo. DLX6-AS1 is significantly dysregulated in various cancers. DLX6-AS1 functions in tumor initiation and progression are regulat...
Source: International Journal of Clinical Chemistry - February 16, 2021 Category: Chemistry Authors: Zheng Q, Gu X, Yang Q, Chu Q, Dai Y, Chen Z Tags: Clin Chim Acta Source Type: research

Utility of serum NOX4 as a potential prognostic biomarker for aneurysmal subarachnoid hemorrhage.
CONCLUSIONS: Serum NOX4 concentrations, in close correlation with hemorrhagic severity, were independently associated with DCI and poor clinical outcome after hemorrhagic stroke, substantializing serum NOX4 as a promising prognostic biomarker for aSAH. PMID: 33607069 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 16, 2021 Category: Chemistry Authors: Pan J, Lao L, Shen J, Huang S, Zhang T, Fan W, Yan M, Gu J, Liu W Tags: Clin Chim Acta Source Type: research

Identification of the first Alu-mediated gross deletion involving the BCKDHA gene in a compound heterozygous patient with maple syrup urine disease.
CONCLUSIONS: This study is the first time report on rearrangement sequences in BCKDHA mediated by Alu element, which resulted in MSUD. Our results may also offer new insights into the formation and pathogenicity of MSUD, and may be useful to genetic counseling and genetic testing. PMID: 33607070 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 16, 2021 Category: Chemistry Authors: Ma S, Zhang Z, Fu Y, Zhang M, Niu Y, Li R, Guo Q, He Z, Zhao Q, Song Z, Wang X, Sun R Tags: Clin Chim Acta Source Type: research

Omentin-1: protective impact on ischemic stroke via ameliorating atherosclerosis.
This study is the first to summarize the comprehensive mechanisms between omentin-1 and atherosclerosis and to review the shielding effect of omentin-1 on IS. We shed light on omentin-1 as a novel therapeutic target for combating IS. PMID: 33607071 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 16, 2021 Category: Chemistry Authors: Lin S, Li X, Zhang J, Zhang Y Tags: Clin Chim Acta Source Type: research

Diagnostic Value of Combining Tumor and Inflammatory Biomarkers in Detecting Common Cancers in Korea.
CONCLUSIONS: Combining multiple tumor and systemic inflammatory biomarkers proved to be a valid strategy in the diagnosis of six common cancers in Korea. Further validation of appropriate screening populations through large-scale clinical trials are warranted. PMID: 33577759 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 9, 2021 Category: Chemistry Authors: Sun Kim Y, Nam Kang K, Sung Shin Y, Eun Lee J, Young Jang J, Woo Kim C Tags: Clin Chim Acta Source Type: research

Identification of novel compound heterozygous variants in SLC19A2 and the genotype-phenotype associations in thiamine-responsive megaloblastic anemia.
CONCLUSION: Two novel compound heterozygous variants (NM_006996.2: exon2:c.336_363del:p.W112fs; exon2:c.358G>T:p.G120X) were identified, and GPAs in TRMA indicated the predictability of clinical manifestations. PMID: 33571483 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 8, 2021 Category: Chemistry Authors: Zhang S, Qiao Y, Wang Z, Zhuang J, Sun Y, Shang X, Li G Tags: Clin Chim Acta Source Type: research

Faecal Immunochemical Tests for Haemoglobin: Analytical Challenges and Potential Solutions.
Abstract Quantitative faecal immunochemical tests for haemoglobin (FIT) are being used increasingly around the world in colorectal cancer screening programmes, and in patients presenting with lower bowel symptoms to determine who should proceed to further bowel visualisation investigations, usually colonoscopy. The clinical utility of FIT is well reported. There are a number of analytical challenges including pre-analytical variation, difficulty setting up external quality assessment schemes, access to third party internal quality control material and a lack of standardisation or harmonisation of FIT methods. Here...
Source: International Journal of Clinical Chemistry - February 8, 2021 Category: Chemistry Authors: Benton SC, Symonds E, Djedovic N, Jones S, Deprez L, Kocna P, Maria Auge J, International Federation of Clinical Chemistry Faecal Immunochemical Test Working Group (IFCC FIT-WG) Tags: Clin Chim Acta Source Type: research

Reciprocal association of serum Mac-2 binding protein and HDL-cholesterol concentrations.
CONCLUSIONS: The serum Mac-2BP concentrations are negatively correlated with HDL-C concentrations in healthy subjects, as a result of chronic inflammation. PMID: 33571485 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 8, 2021 Category: Chemistry Authors: Uekita H, Yamamoto H, Niinaga R, Yamane N, Yoshii M, Yamauchi-Takihara K, Kihara S Tags: Clin Chim Acta Source Type: research

UriSed 3 PRO automated microscope in screening bacteriuria at region-wide laboratory organization.
CONCLUSIONS: Complex patient populations, regional logistics and data interphases, and economics related to increased costs of additional particle counts against costs of screening cultures of all samples, did not support adaptation of a screening process of urine cultures. This conclusion was made locally, and may not be valid elsewhere. PMID: 33549597 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 4, 2021 Category: Chemistry Authors: Kouri T, Holma T, Kirjavainen V, Lempiäinen A, Alagrund K, Tohmola N, Pihlajamaa T, Kouri VP, Lehtonen M, Friman S, Pätäri-Sampo A Tags: Clin Chim Acta Source Type: research

Using MALDI-TOF mass spectrometry in peripheral blood for the follow up of newly diagnosed multiple myeloma patients treated with daratumumab-based combination therapy.
Abstract BACKGROUND: Daratumumab-based combination therapies have shown high rates of complete response (CR) and minimal residual disease negativity in patients with multiple myeloma. However, daratumumab, an IgGĶ monoclonal antibody, interferes with electrophoretic techniques making it difficult to reliably define residual disease versus CR, especially in patients with IgGĶ multiple myeloma. METHODS: Enrichment with polyclonal sheep antibody-coated magnetic microparticles combined with MALDI-TOF mass spectrometry (MALDI-TOF MS) analysis was used to detect M-proteins in serial samples from newly diagnosed m...
Source: International Journal of Clinical Chemistry - February 2, 2021 Category: Chemistry Authors: Eveillard M, Korde N, Ciardiello A, Diamond B, Lesokhin A, Mailankody S, Smith E, Hassoun H, Hultcrantz M, Shah U, Lu S, Salcedo M, Werner K, Rispoli J, Mastey D, Landgren O, Thoren K Tags: Clin Chim Acta Source Type: research

MicroRNA in Multiple Sclerosis.
Abstract Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system (CNS). Despite a complex pathogenesis, it appears that an imbalanced immune system plays an important role in the disease process. MicroRNAs (miRNAs) are comprised of short non-coding single-stranded molecules mainly involved in regulating gene expression through the inhibition of transcription and translation. miRNAs are key regulatory molecules in the nucleus and participate in the proliferation, differentiation, and apoptosis of various cells throughout the body. Recent studies, however, have found that miRNAs are ...
Source: International Journal of Clinical Chemistry - February 2, 2021 Category: Chemistry Authors: Gao Y, Han D, Feng J Tags: Clin Chim Acta Source Type: research

Electrochemical prostate-specific antigen biosensors based on electroconductive nanomaterials and polymers.
This article reviews electrochemical PSA biosensors using electroconductive nanomaterials such as carbon-, metal-, metal oxide- and peptide-based nanostructures, as well as polymers to significantly improve conductivity and enhance sensitivity. Challenges associated with the development of these devices are discussed thus providing additional insight into their analytic strength as well as their potential use in early PCa detection. PMID: 33545110 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - February 2, 2021 Category: Chemistry Authors: Dowlatshahi S, Abdekhodaie MJ Tags: Clin Chim Acta Source Type: research

Recent advances in the regulation of ABCA1 and ABCG1 by lncRNAs.
Abstract Coronary heart disease (CHD) with atherosclerosis is the leading cause of death worldwide. ABCA1 and ABCG1 promote cholesterol efflux to suppress foam cell generation and reduce atherosclerosis development. Long noncoding RNAs (lncRNAs) are emerging as a unique group of RNA transcripts that longer than 200 nucleotides and have no protein-coding potential. Many studies have found that lncRNAs regulate cholesterol efflux to influence atherosclerosis development. ABCA1 is regulated by different lncRNAs, including MeXis, GAS5, TUG1, MEG3, MALAT1, Lnc-HC, RP5-833A20.1, LOXL1-AS1, CHROME, DAPK1-IT1, SIRT1 AS ln...
Source: International Journal of Clinical Chemistry - February 2, 2021 Category: Chemistry Authors: Zhang S, Li L, Wang J, TingtingZhang, TingYe, Wang S, Xing D, Chen W Tags: Clin Chim Acta Source Type: research

Verification of UriSed 3 PRO automated urine microscope in regional laboratory environment.
CONCLUSIONS: UriSed 3 PRO instruments were adopted into routine use after acceptance of the verification. PMID: 33460593 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - January 15, 2021 Category: Chemistry Authors: Kouri T, Alagrund K, Lehtonen M, Tohmola N, Pihlajamaa T, Kouri VP, Friman S, Holma T, Kirjavainen V, Pätäri-Sampo A, Lempiäinen A Tags: Clin Chim Acta Source Type: research

Extracellular vesicle-transferred long noncoding RNAs in bladder cancer.
In conclusion, BC-associated EV-transferred lncRNAs show significant potential as noninvasive biomarkers or therapeutic targets for BC diagnosis and treatment. PMID: 33450212 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - January 12, 2021 Category: Chemistry Authors: Xue M, Chen W, Li X Tags: Clin Chim Acta Source Type: research

Evaluation of the necessity and the feasibility of the standardization of procalcitonin measurements: activities of IFCC WG-PCT with involvement of all stakeholders.
Abstract Procalcitonin (PCT) is an important biomarker for sepsis diagnosis and management. To date, there is no higher-order reference measurement procedure (RMP) and certified reference material to achieve global standardization of results and results traceability to the SI units. Although efforts have been made to harmonize PCT results, a number of correlation studies and external quality assessment (EQA) schemes show conflicting results regarding results comparability and to date, equivalence of PCT results across the assays remains questionable in absence of studies relying on commutable EQA materials. In thi...
Source: International Journal of Clinical Chemistry - January 12, 2021 Category: Chemistry Authors: Huynh HH, Boeuf A, Vinh J, Delatour V, IFCC Working Group on Standardization of Procalcitonin assays WG-PCT Tags: Clin Chim Acta Source Type: research

The Epitranscriptome of Long Noncoding RNAs in Metabolic Diseases.
Yin K Abstract Long noncoding RNAs (lncRNAs) have abundant content and extensive functions that regulate the expression of genes at multiple levels. Recently, transcriptome-wide analysis confirmed that RNA can undergo various chemical modifications in response to stimulation by the environment that further determine the action mechanisms of RNAs and expand the diversity of the transcriptome. Modifications that occur in lncRNAs can affect their expression and the regulation of downstream molecules by changing the secondary structure, splicing, degradation or molecular stability of lncRNAs. During the development of...
Source: International Journal of Clinical Chemistry - January 7, 2021 Category: Chemistry Authors: Yin L, Zhu X, Novák P, Zhou L, Gao L, Yang M, Zhao G, Yin K Tags: Clin Chim Acta Source Type: research

Cross-reactive peptide epitopes of Enterovirus Coxsackie B4 and human Glutamic Acid Decarboxylase detecting antibodies in Latent Autoimmune Diabetes in Adults versus Type 1 Diabetes.
CONCLUSIONS: Our study highlights the usefulness of peptides as diagnostic antigens in T1DM and LADA, and extends previous findings by comparing IgM and IgG-isotype antibodies in the same population. Additionally, results highlight the role of the entourage in the shared sequon PEVKXK in GAD and CVB4P2C particularly in IgMs identification. PMID: 33422493 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - January 7, 2021 Category: Chemistry Authors: Real-Fernández F, Gallo A, Nuti F, Altamore L, Giovanna Del Vescovo G, Traldi P, Ragazzi E, Rovero P, Lapolla A, Maria Papini A Tags: Clin Chim Acta Source Type: research

The path to the standardization of PTH: Is this a realistic possibility? A position paper of the IFCC C-BM.
Abstract Parathyroid hormone (PTH) determination is of greatest importance for patients suffering from parathyroid gland disorders and for the follow-up of bone turnover in patients suffering from chronic kidney disease (CKD). Two generations of PTH assays are simultaneously present on the market for PTH quantification. As these assays are not yet standardized, this results in a significant level of confusion in the care of CKD patients. One key objective of the IFCC Committee for Bone Metabolism is to improve this situation. In this position paper, we will highlight the current state of PTH testing and propose a ...
Source: International Journal of Clinical Chemistry - January 4, 2021 Category: Chemistry Authors: Cavalier E, Vasikaran S, Bhattoa HP, Heijboer AC, Makris K, Ulmer CZ Tags: Clin Chim Acta Source Type: research

Mucopolysaccharidosis type VII as a cause of recurrent non-immune hydrops fetalis: The first Tunisian case confirmed by next-generation sequencing.
In this report, we describe the first Tunisian case of Mucopolysaccharidosis type VII caused by a homozygous mutation in the GUSB gene confirmed by a next-generation sequencing gene panel in a patient with recurrent Non-immune hydrops fetalis. PMID: 33382994 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 28, 2020 Category: Chemistry Authors: Hizem S, Elaribi Y, Ben Slama S, Dimassi K, Jilani H, Rejeb I, Sebai M, Bekri S, Ben Jemaa L Tags: Clin Chim Acta Source Type: research

Analytical considerations and plans to standardize or harmonize assays for the reference bone turnover markers PINP and β-CTX in blood.
Analytical considerations and plans to standardize or harmonize assays for the reference bone turnover markers PINP and β-CTX in blood. Clin Chim Acta. 2020 Dec 28;: Authors: Bhattoa HP, Cavalier E, Eastell R, Heijboer AC, Jørgensen NR, Makris K, Ulmer CZ, Kanis JA, Cooper C, Silverman SL, Vasikaran SD, IFCC-IOF Committee for Bone Metabolism Abstract Procollagen type I N-propeptide (PINP) and the C-terminal telopeptide of type I collagen (β-CTX) in blood have been designated as reference bone turnover markers in osteoporosis by the International Osteoporosis Foundation (IOF) and Inter...
Source: International Journal of Clinical Chemistry - December 28, 2020 Category: Chemistry Authors: Bhattoa HP, Cavalier E, Eastell R, Heijboer AC, Jørgensen NR, Makris K, Ulmer CZ, Kanis JA, Cooper C, Silverman SL, Vasikaran SD, IFCC-IOF Committee for Bone Metabolism Tags: Clin Chim Acta Source Type: research

Updated specification to a plasma sodium level-corrected erythrocyte mean corpuscular volume report standard when using the Sysmex XE 2100.
CONCLUSIONS: We recommend reporting clinically significant corrected MCVs, based on sample pre-dilution with the Sysmex buffer, when the proposed composite metabolic flag is triggered. PMID: 33359494 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 23, 2020 Category: Chemistry Authors: Zhan SH, Nicolson H, Takahashi A, Mattman A Tags: Clin Chim Acta Source Type: research

Spheroplasts, poorly known but clinically relevant particles of urinary sediment.
CONCLUSION: Appropriate training of urine laboratory professionals in spheroplast detection and association with UTI, together with timely communication with the microbiologist and caring clinician, will provide prompt targeted treatment. PMID: 33359495 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 23, 2020 Category: Chemistry Authors: Falbo R, Battista Fogazzi G, Roberta Sala M, Garigali G, AdelaSulejmani, Brambilla P, Leoni V Tags: Clin Chim Acta Source Type: research

The evolving role of commutability in metrological traceability.
We describe how commutability was recognized as a critical requirement for metrological traceability and we present recommendations from the IFCC Working Groups on Commutability and on Commutability in Metrological Traceability. PMID: 33359496 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 23, 2020 Category: Chemistry Authors: Greg Miller W, Greenberg N, Budd J, Delatour V, IFCC Working Group on Commutability in Metrological Traceability Tags: Clin Chim Acta Source Type: research

Standardization process of continuous glucose monitoring: traceability and performance.
Abstract People with diabetes are required to regularly check their glucose to make therapy decisions. So far, systems for self-monitoring of blood glucose were used, but nowadays minimally invasive continuous glucose monitoring (CGM) systems are increasingly more often employed, sometimes to partially replace self-monitoring of blood glucose. Most CGM systems on the market measure glucose concentrations continuously in the interstitial fluid of the subcutaneous fatty tissue. However, CGM has a principle limitation. Collecting interstitial fluid frequently in sufficiently large volumes over short time periods is n...
Source: International Journal of Clinical Chemistry - December 23, 2020 Category: Chemistry Authors: Freckmann G, Nichols JH, Hinzmann R, Klonoff DC, Ju Y, Diem P, Makris K, Slingerland RJ Tags: Clin Chim Acta Source Type: research

X-linked adrenoleukodystrophy caused by a novel mutation presenting with various phenotypes in a Taiwanese family.
We reported a 46-year-old male admitted to Department of Neurology, Chang Gung Memorial Hospital with progressive paraparesis and Addison's disease, which was diagnosed when he was around 20-year-old. Plasma levels of VLCFA showed that his C26:0, C24:0/C22:0 and C26:0/C22:0 ratios were significantly elevated. A novel missense mutation (p.Arg163Cys) caused by the nucleotide change c.487C>T in exon 1 was identified in the ABCD1 gene of the proband and his subclinical family members. In this article, we reviewed the mutations that had been reported at the same position with different phenotypes. Given that the nerve conduc...
Source: International Journal of Clinical Chemistry - December 22, 2020 Category: Chemistry Authors: Chien CY, Chang KH, Chen CM Tags: Clin Chim Acta Source Type: research

Circulating macroprolactin exhibits molecular heterogeneity and is not exclusively an antibody complex.
We examined macPRL heterogeneity in samples from thirteen patients suspected of macroprolactinemia. METHODS: Polyethylene glycol (PEG) precipitation, gel permeation (GPC), protein-G affinity, and Lectin affinity chromatography were used to investigate the nature of macPRL. RESULTS: Using PEG, 8, 3, and 2 samples were macPRL positive, negative, and indeterminate respectively. Using GPC, prolactin appeared at high (H) (≥150 kDa), mid (M) (≥30
Source: International Journal of Clinical Chemistry - December 22, 2020 Category: Chemistry Authors: Quynh N Nguyen K, Langevin RH, McPhaul MJ, Hashim IA Tags: Clin Chim Acta Source Type: research

We need to talk about the analytical performance of our laboratory developed clinical LC-MS/MS tests, and start separating the wheat from the chaff.
Abstract With the upcoming EU regulation on the use of in-vitro diagnostic devices, a critical evaluation of the current status of our in-house developed LC-MS/MS methods is timely and of great relevance. Recently, much attention has been devoted to the need for better specification of analytical and clinical performance. Appropriate reporting of the actual achieved analytical performance is an important determinant of the clinical performance and subsequent clinical effectiveness of a test. We advocate for the application of CLSI C62-A guidelines for method validation and suggest some adaptations for analytical v...
Source: International Journal of Clinical Chemistry - December 22, 2020 Category: Chemistry Authors: Dirks NF, Ackermans MT, Martens F, Cobbaert CM, de Jonge R, Heijboer AC Tags: Clin Chim Acta Source Type: research

Lipid-lowering therapy: guidelines to precision medicine.
Abstract Dyslipidemia is correlated with a series of health problems, such as obesity, insulin resistance, and the development of cardiovascular disease (CVD). Currently, accumulating evidence sheds light on the fact that β-hydroxy β-methylglutaryl-CoA reductase inhibitors, named statins, could lower circulating lipid-density lipoprotein cholesterol (LDL-C) and represent a revolution for the prevention of metabolic disorder diseases. In addition, statins remain the cornerstone of LDL-C-lowering treatments, together with ezetimibe and bile acid sequestrants, which are used either in combination with stati...
Source: International Journal of Clinical Chemistry - December 22, 2020 Category: Chemistry Authors: Su X, Cheng Y, Chang D Tags: Clin Chim Acta Source Type: research

cStabilization of urinary biogenic amines measured in clinical chemistry laboratories.
In conclusion, sodium citrate is a non-hazardous alternative to HCl for patients to send unfrozen urine samples to the laboratory which may safely store the sample for four weeks. PMID: 33333038 [PubMed - as supplied by publisher] (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - December 14, 2020 Category: Chemistry Authors: Eugster PJ, Centeno C, Dunand M, Seghezzi C, Grouzmann E Tags: Clin Chim Acta Source Type: research