Identification and structure characterization of novel IDS variants causing mucopolysaccharidosis type II: A retrospective analysis of 30 Chinese children
CONCLUSIONS: Our study expands the spectrum of MPS II genotype, provides new insights into the molecular mechanisms of MPS II, and contributes to future studies of genotype-phenotypic associations to estimate prognosis and develop new treatment regimens.PMID:34670126 | DOI:10.1016/j.cca.2021.10.020 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Xiao- Ying Zhao Guang-Ming Qiao Fang Liu Source Type: research

Evaluation of thrombin generation assay in factor XI deficiency
Clin Chim Acta. 2021 Oct 17:S0009-8981(21)00365-X. doi: 10.1016/j.cca.2021.10.021. Online ahead of print.ABSTRACTINTRODUCTION: Factor XI (FXI) deficiency is characterized by a lack of correlation between FXI plasma levels and the occurrence of hemorrhagic events. The main objective of our study was to determine whether thrombin generation assay (TGA) could be used to assess the hemorrhagic phenotype of patients with FXI deficiency.MATERIAL AND METHODS: All patients had confirmed laboratory measurement of FXI
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Fiston Kasonga Guillaume Feugray Pierre Chamouni Virginie Barbay Marielle Fresel Marie H élène Chretien Sabine Brunel V éronique LE Cam Duchez Paul Billoir Source Type: research

Identification and structure characterization of novel IDS variants causing mucopolysaccharidosis type II: A retrospective analysis of 30 Chinese children
CONCLUSIONS: Our study expands the spectrum of MPS II genotype, provides new insights into the molecular mechanisms of MPS II, and contributes to future studies of genotype-phenotypic associations to estimate prognosis and develop new treatment regimens.PMID:34670126 | DOI:10.1016/j.cca.2021.10.020 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Xiao- Ying Zhao Guang-Ming Qiao Fang Liu Source Type: research

Evaluation of thrombin generation assay in factor XI deficiency
Clin Chim Acta. 2021 Oct 17:S0009-8981(21)00365-X. doi: 10.1016/j.cca.2021.10.021. Online ahead of print.ABSTRACTINTRODUCTION: Factor XI (FXI) deficiency is characterized by a lack of correlation between FXI plasma levels and the occurrence of hemorrhagic events. The main objective of our study was to determine whether thrombin generation assay (TGA) could be used to assess the hemorrhagic phenotype of patients with FXI deficiency.MATERIAL AND METHODS: All patients had confirmed laboratory measurement of FXI
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Fiston Kasonga Guillaume Feugray Pierre Chamouni Virginie Barbay Marielle Fresel Marie H élène Chretien Sabine Brunel V éronique LE Cam Duchez Paul Billoir Source Type: research

Identification and structure characterization of novel IDS variants causing mucopolysaccharidosis type II: A retrospective analysis of 30 Chinese children
CONCLUSIONS: Our study expands the spectrum of MPS II genotype, provides new insights into the molecular mechanisms of MPS II, and contributes to future studies of genotype-phenotypic associations to estimate prognosis and develop new treatment regimens.PMID:34670126 | DOI:10.1016/j.cca.2021.10.020 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Xiao- Ying Zhao Guang-Ming Qiao Fang Liu Source Type: research

Evaluation of thrombin generation assay in factor XI deficiency
Clin Chim Acta. 2021 Oct 17:S0009-8981(21)00365-X. doi: 10.1016/j.cca.2021.10.021. Online ahead of print.ABSTRACTINTRODUCTION: Factor XI (FXI) deficiency is characterized by a lack of correlation between FXI plasma levels and the occurrence of hemorrhagic events. The main objective of our study was to determine whether thrombin generation assay (TGA) could be used to assess the hemorrhagic phenotype of patients with FXI deficiency.MATERIAL AND METHODS: All patients had confirmed laboratory measurement of FXI
Source: International Journal of Clinical Chemistry - October 20, 2021 Category: Chemistry Authors: Fiston Kasonga Guillaume Feugray Pierre Chamouni Virginie Barbay Marielle Fresel Marie H élène Chretien Sabine Brunel V éronique LE Cam Duchez Paul Billoir Source Type: research

Gut Microbiome and Metabolic response in Non-Alcoholic Fatty Liver Disease
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00358-2. doi: 10.1016/j.cca.2021.10.014. Online ahead of print.ABSTRACTFatty liver disease (FLD) is one of the largest burdens to human health worldwide and is associated with gut microbiome and metabolite stability. Engineered liver tissues have shown promise in restoring liver functions in non-alcoholic FLD (NAFLD), hepatitis and cirrhosis. Fatty liver, largely noted in obesity and hepatic cancer, is highly fatal and has led to a global increase in death rates. It is associated with complex metabolic reprogramming too. A standard approach to therapy in the newly diagnosed setting...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Asmita Madatali Abuwani Shweta Priyadarshini Dash Raja Ganesan Kaviyarasi Renu Vellingiri Balachandar Kandasamy Sabariswaran C R Sundara Rajan Abilash Valsala Gopalakrishnan Source Type: research

Setting analytical performance specifications using HbA1c as a model measurand
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00360-0. doi: 10.1016/j.cca.2021.10.016. Online ahead of print.ABSTRACTAnalytical performance specifications (APS) for measurands describe the minimum analytical quality requirements for their measurement. These APS are used to monitor and contain the systematic (trueness/ bias) and random errors (precision/ imprecision) of a laboratory measurement to ensure the results are "fit for purpose" in informing clinical decisions about managing a patient's health condition. In this review, we highlighted the wide variation in the setting of APS, using different levels of evidenc...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Tze Ping Loh Alison F Smith Katy Jl Bell Sarah J Lord Ferruccio Ceriotti Graham Jones Patrick Bossuyt Sverre Sandberg Andrea Rita Horvath European Federation of Clinical Chemistry, Laboratory Medicine Task Group on Outcome-based Analytical Performance Spe Source Type: research

Urinary podocyte markers in kidney diseases
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00361-2. doi: 10.1016/j.cca.2021.10.017. Online ahead of print.ABSTRACTPodocytes play an important role in the maintenance of kidney function, and they are the primary focus of many kidney diseases. Podocyte injury results in the shedding of podocyte-derived cellular fragments and podocyte-specific molecular targets into the urine, which may serve as biomarkers of kidney diseases. Intact podocytes, either viable or dead, and podocyte-derived microvesicles could be quantified in the urine by various centrifugation, visualization and culture methods. Podocyte-specific protein targets...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Lingfeng Zeng Cheuk-Chun Szeto Source Type: research

Urinary neutrophil gelatinase-associated lipocalin: acute kidney injury in liver cirrhosis
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00359-4. doi: 10.1016/j.cca.2021.10.015. Online ahead of print.ABSTRACTAcute kidney injury (AKI) in liver cirrhosis is associated with poor clinical outcomes including an increased long-and short-term mortality. The common type of AKI observed in patients with cirrhosis are prerenal AKI (PRA), hepatorenal syndrome (HRS) and acute tubular necrosis (ATN). Despite growing knowledge and uniform definition for the diagnosis of AKI, there are several challenges including early diagnosis and management. Precisely differentiating the type of AKI is critical, as therapies differ significant...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Pooja Basthi Mohan Shankar Prasad Nagaraju Dharshan Rangaswamy Balaji Musunuri Ravindra Prabhu Attur Ganesh Bhat None Shailesh Shiran Shetty Source Type: research

Determination of 97.5 < sup > th < /sup > and 99 < sup > th < /sup > Percentile Upper Reference Limits for Heart-type Fatty Acid-Binding Protein (H-FABP) in a US Population
CONCLUSION: As the URL for H-FABP is highly affected by population distribution and outlier removal, final determination for an assay cutoff should be made in the context of clinical utility, either as a standalone assay or in conjunction with other biomarkers, and the desired clinical sensitivity and specificity.PMID:34666029 | DOI:10.1016/j.cca.2021.10.011 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Michael A Vera Christopher D Koch Peter A Kavsak Joe M El-Khoury Source Type: research

Exosomes in nasopharyngeal carcinoma
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00357-0. doi: 10.1016/j.cca.2021.10.013. Online ahead of print.ABSTRACTNasopharyngeal carcinoma (NPC) is a malignant epithelial tumor with a unique geographical distribution, primarily prevalent in East Africa and Asia. Although there is an increased understanding of the pathogenesis and risk factors of NPC, prevention and treatment efforts remain limited. Various studies have indicated that exosomes are actively involved in NPC by delivering biomolecules such as non-coding RNAs and proteins to target cells. In this review, we summarize the biological functions of exosomes in NPC a...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Liu Yiwen Wen Jianping Huang Weiguo Source Type: research

Sestrin2 in atherosclerosis
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00363-6. doi: 10.1016/j.cca.2021.10.019. Online ahead of print.ABSTRACTAtherosclerosis (AS) is the pathological basis of numerous lethal diseases, such as myocardial infarction, heart failure, and stroke. As we know, almost twenty million people worldwide die of the arterial diseases annually. Sestrin2 is a stress-inducing protein, which serves as a guardian by activating AMPK, inhibiting mTOR, and maintaining redox balance beneath various stress environments. A large number of studies show that Sestrin2 would shield the body from injury by stress. Moreover, it has been demonstrate...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Zhen Tian Bin-Jie Yan Wen Luo Dan-Dan Gui Kun Zhou Kai-Jiang Tian Yun Ma Zhi-Xiang Zhou Zhi-Sheng Jiang Source Type: research

Novel variants in OSGEP leading to Galloway-Mowat syndrome by altering its subcellular localization
In this study, two siblings mainly presenting with decreased head circumference, hypotonia, gross motor delay, and dysmorphic features were initially detected without pathogenic variants by karyotyping, SNP-array and WES. After a 3 year's follow-up, the proband manifested additional proteinuria, hematuria and "deeper sulci" with a sign of brain atrophy. By reanalysis on the proband's previous WES data, two novel compound heterozygous variants of OSGEP (c.133dupA; c.608C>T) were identified. Furthermore, functional studies showed that the variants reduced the expression of OSGEP protein and activated the DNA dam...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Hua Teng Chen Liang Desheng Liang Zhuo Li Lingqian Wu Source Type: research

Gut Microbiome and Metabolic response in Non-Alcoholic Fatty Liver Disease
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00358-2. doi: 10.1016/j.cca.2021.10.014. Online ahead of print.ABSTRACTFatty liver disease (FLD) is one of the largest burdens to human health worldwide and is associated with gut microbiome and metabolite stability. Engineered liver tissues have shown promise in restoring liver functions in non-alcoholic FLD (NAFLD), hepatitis and cirrhosis. Fatty liver, largely noted in obesity and hepatic cancer, is highly fatal and has led to a global increase in death rates. It is associated with complex metabolic reprogramming too. A standard approach to therapy in the newly diagnosed setting...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Asmita Madatali Abuwani Shweta Priyadarshini Dash Raja Ganesan Kaviyarasi Renu Vellingiri Balachandar Kandasamy Sabariswaran C R Sundara Rajan Abilash Valsala Gopalakrishnan Source Type: research

Setting analytical performance specifications using HbA1c as a model measurand
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00360-0. doi: 10.1016/j.cca.2021.10.016. Online ahead of print.ABSTRACTAnalytical performance specifications (APS) for measurands describe the minimum analytical quality requirements for their measurement. These APS are used to monitor and contain the systematic (trueness/ bias) and random errors (precision/ imprecision) of a laboratory measurement to ensure the results are "fit for purpose" in informing clinical decisions about managing a patient's health condition. In this review, we highlighted the wide variation in the setting of APS, using different levels of evidenc...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Tze Ping Loh Alison F Smith Katy Jl Bell Sarah J Lord Ferruccio Ceriotti Graham Jones Patrick Bossuyt Sverre Sandberg Andrea Rita Horvath European Federation of Clinical Chemistry, Laboratory Medicine Task Group on Outcome-based Analytical Performance Spe Source Type: research

Urinary podocyte markers in kidney diseases
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00361-2. doi: 10.1016/j.cca.2021.10.017. Online ahead of print.ABSTRACTPodocytes play an important role in the maintenance of kidney function, and they are the primary focus of many kidney diseases. Podocyte injury results in the shedding of podocyte-derived cellular fragments and podocyte-specific molecular targets into the urine, which may serve as biomarkers of kidney diseases. Intact podocytes, either viable or dead, and podocyte-derived microvesicles could be quantified in the urine by various centrifugation, visualization and culture methods. Podocyte-specific protein targets...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Lingfeng Zeng Cheuk-Chun Szeto Source Type: research

Urinary neutrophil gelatinase-associated lipocalin: acute kidney injury in liver cirrhosis
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00359-4. doi: 10.1016/j.cca.2021.10.015. Online ahead of print.ABSTRACTAcute kidney injury (AKI) in liver cirrhosis is associated with poor clinical outcomes including an increased long-and short-term mortality. The common type of AKI observed in patients with cirrhosis are prerenal AKI (PRA), hepatorenal syndrome (HRS) and acute tubular necrosis (ATN). Despite growing knowledge and uniform definition for the diagnosis of AKI, there are several challenges including early diagnosis and management. Precisely differentiating the type of AKI is critical, as therapies differ significant...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Pooja Basthi Mohan Shankar Prasad Nagaraju Dharshan Rangaswamy Balaji Musunuri Ravindra Prabhu Attur Ganesh Bhat None Shailesh Shiran Shetty Source Type: research

Determination of 97.5 < sup > th < /sup > and 99 < sup > th < /sup > Percentile Upper Reference Limits for Heart-type Fatty Acid-Binding Protein (H-FABP) in a US Population
CONCLUSION: As the URL for H-FABP is highly affected by population distribution and outlier removal, final determination for an assay cutoff should be made in the context of clinical utility, either as a standalone assay or in conjunction with other biomarkers, and the desired clinical sensitivity and specificity.PMID:34666029 | DOI:10.1016/j.cca.2021.10.011 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Michael A Vera Christopher D Koch Peter A Kavsak Joe M El-Khoury Source Type: research

Exosomes in nasopharyngeal carcinoma
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00357-0. doi: 10.1016/j.cca.2021.10.013. Online ahead of print.ABSTRACTNasopharyngeal carcinoma (NPC) is a malignant epithelial tumor with a unique geographical distribution, primarily prevalent in East Africa and Asia. Although there is an increased understanding of the pathogenesis and risk factors of NPC, prevention and treatment efforts remain limited. Various studies have indicated that exosomes are actively involved in NPC by delivering biomolecules such as non-coding RNAs and proteins to target cells. In this review, we summarize the biological functions of exosomes in NPC a...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Liu Yiwen Wen Jianping Huang Weiguo Source Type: research

Sestrin2 in atherosclerosis
Clin Chim Acta. 2021 Oct 16:S0009-8981(21)00363-6. doi: 10.1016/j.cca.2021.10.019. Online ahead of print.ABSTRACTAtherosclerosis (AS) is the pathological basis of numerous lethal diseases, such as myocardial infarction, heart failure, and stroke. As we know, almost twenty million people worldwide die of the arterial diseases annually. Sestrin2 is a stress-inducing protein, which serves as a guardian by activating AMPK, inhibiting mTOR, and maintaining redox balance beneath various stress environments. A large number of studies show that Sestrin2 would shield the body from injury by stress. Moreover, it has been demonstrate...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Zhen Tian Bin-Jie Yan Wen Luo Dan-Dan Gui Kun Zhou Kai-Jiang Tian Yun Ma Zhi-Xiang Zhou Zhi-Sheng Jiang Source Type: research

Novel variants in OSGEP leading to Galloway-Mowat syndrome by altering its subcellular localization
In this study, two siblings mainly presenting with decreased head circumference, hypotonia, gross motor delay, and dysmorphic features were initially detected without pathogenic variants by karyotyping, SNP-array and WES. After a 3 year's follow-up, the proband manifested additional proteinuria, hematuria and "deeper sulci" with a sign of brain atrophy. By reanalysis on the proband's previous WES data, two novel compound heterozygous variants of OSGEP (c.133dupA; c.608C>T) were identified. Furthermore, functional studies showed that the variants reduced the expression of OSGEP protein and activated the DNA dam...
Source: International Journal of Clinical Chemistry - October 19, 2021 Category: Chemistry Authors: Hua Teng Chen Liang Desheng Liang Zhuo Li Lingqian Wu Source Type: research

Soluble triggering receptor expressed on myeloid cells-1 as a serum biomarker of early neurologic deterioration and prognosis in acute supratentorial intracerebral hemorrhage
CONCLUSION: Serum sTREM-1 may represent a promising inflammatory biomarker for assessment of severity and prediction of END and poor outcome after ICH.PMID:34655609 | DOI:10.1016/j.cca.2021.10.010 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 16, 2021 Category: Chemistry Authors: Yingrui Gu Xiaodong Deng Chong Liang Yi Chen Hongbing Lei Qi Zhang Source Type: research

Soluble triggering receptor expressed on myeloid cells-1 as a serum biomarker of early neurologic deterioration and prognosis in acute supratentorial intracerebral hemorrhage
CONCLUSION: Serum sTREM-1 may represent a promising inflammatory biomarker for assessment of severity and prediction of END and poor outcome after ICH.PMID:34655609 | DOI:10.1016/j.cca.2021.10.010 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 16, 2021 Category: Chemistry Authors: Yingrui Gu Xiaodong Deng Chong Liang Yi Chen Hongbing Lei Qi Zhang Source Type: research

Soluble triggering receptor expressed on myeloid cells-1 as a serum biomarker of early neurologic deterioration and prognosis in acute supratentorial intracerebral hemorrhage
CONCLUSION: Serum sTREM-1 may represent a promising inflammatory biomarker for assessment of severity and prediction of END and poor outcome after ICH.PMID:34655609 | DOI:10.1016/j.cca.2021.10.010 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 16, 2021 Category: Chemistry Authors: Yingrui Gu Xiaodong Deng Chong Liang Yi Chen Hongbing Lei Qi Zhang Source Type: research

A capillary electrophoresis-based variant hotspot genotyping method for rapid and reliable analysis of the phenylalanine hydroxylase gene in the Chinese Han population
CONCLUSION: In summary, SNaPshot can accurately and rapidly detect PAH gene variants at a comparable performance and lower cost as compared with NGS. Our results suggest that SNaPshot may serve as a promising approach for a routine genetic test in clinical practice.PMID:34653385 | DOI:10.1016/j.cca.2021.10.009 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 15, 2021 Category: Chemistry Authors: Binbin Shao An Liu Jingjing Zhang Yan Wang Fengchang Qiao Cuiping Zhang Yuqing Zhu Yingchun Lin Ping Hu Tao Tao Zhengwen Jiang Jianxin Tan Zhengfeng Xu Source Type: research

Opinion paper Problems with estimating reference change values (critical differences)
Clin Chim Acta. 2021 Oct 12:S0009-8981(21)00350-8. doi: 10.1016/j.cca.2021.10.006. Online ahead of print.ABSTRACTThe concept of reference change values (RCVs) for diagnosis and monitoring of diseases has become well established. Several models habe been developed, e. g. one assuming a normal distribution and another one for a log-normal distribution. RCV values calculated for some measurands with both models are compared with each other and led to similar results. A few examples led to RCV values which are not plausible for diagnostic purposes. Although statistically# the RCV values are well established, their clinical rel...
Source: International Journal of Clinical Chemistry - October 15, 2021 Category: Chemistry Authors: Rainer Haeckel Anna Carobene Werner Wosniok Source Type: research

A capillary electrophoresis-based variant hotspot genotyping method for rapid and reliable analysis of the phenylalanine hydroxylase gene in the Chinese Han population
CONCLUSION: In summary, SNaPshot can accurately and rapidly detect PAH gene variants at a comparable performance and lower cost as compared with NGS. Our results suggest that SNaPshot may serve as a promising approach for a routine genetic test in clinical practice.PMID:34653385 | DOI:10.1016/j.cca.2021.10.009 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 15, 2021 Category: Chemistry Authors: Binbin Shao An Liu Jingjing Zhang Yan Wang Fengchang Qiao Cuiping Zhang Yuqing Zhu Yingchun Lin Ping Hu Tao Tao Zhengwen Jiang Jianxin Tan Zhengfeng Xu Source Type: research

Opinion paper Problems with estimating reference change values (critical differences)
Clin Chim Acta. 2021 Oct 12:S0009-8981(21)00350-8. doi: 10.1016/j.cca.2021.10.006. Online ahead of print.ABSTRACTThe concept of reference change values (RCVs) for diagnosis and monitoring of diseases has become well established. Several models habe been developed, e. g. one assuming a normal distribution and another one for a log-normal distribution. RCV values calculated for some measurands with both models are compared with each other and led to similar results. A few examples led to RCV values which are not plausible for diagnostic purposes. Although statistically# the RCV values are well established, their clinical rel...
Source: International Journal of Clinical Chemistry - October 15, 2021 Category: Chemistry Authors: Rainer Haeckel Anna Carobene Werner Wosniok Source Type: research

Additive value of bioclinical risk scores to high sensitivity troponins-only strategy in acute coronary syndrome
CONCLUSION: In view of our results, the decision aids using only biological variables (hs-cTn-only strategy and CCS) would seem more effective for rule-out AMI whereas bioclinical risk scores could better identify patients at low and high risk for mortality. In consequence, risk scores taking in account comorbidities, appear necessary to determine the outcome and thus to adapt the therapeutic options. It is interesting to note that the HEART score could be useful for the rule out AMI but also for the risk prediction as confirmed by the NRI.PMID:34648808 | DOI:10.1016/j.cca.2021.10.008 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 14, 2021 Category: Chemistry Authors: Anne Marie Dupuy Gr égoire Pasquier Louis Thiebaut Fran çois Roubille Mustapha Sebbane Jean Paul Cristol Source Type: research

Additive value of bioclinical risk scores to high sensitivity troponins-only strategy in acute coronary syndrome
CONCLUSION: In view of our results, the decision aids using only biological variables (hs-cTn-only strategy and CCS) would seem more effective for rule-out AMI whereas bioclinical risk scores could better identify patients at low and high risk for mortality. In consequence, risk scores taking in account comorbidities, appear necessary to determine the outcome and thus to adapt the therapeutic options. It is interesting to note that the HEART score could be useful for the rule out AMI but also for the risk prediction as confirmed by the NRI.PMID:34648808 | DOI:10.1016/j.cca.2021.10.008 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 14, 2021 Category: Chemistry Authors: Anne Marie Dupuy Gr égoire Pasquier Louis Thiebaut Fran çois Roubille Mustapha Sebbane Jean Paul Cristol Source Type: research

Quantitative analysis of urine acylglycines by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS): reference intervals and disease specific patterns in individuals with organic acidemias and fatty acid oxidation disorders
CONCLUSION: This manuscript extends our previously published method by providing the reference ranges and disease specific increases and patterns of urine acylglycine species using the largest data set published to date.PMID:34634292 | DOI:10.1016/j.cca.2021.10.007 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 11, 2021 Category: Chemistry Authors: Judith A Hobert Irene De Biase Tatiana Yuzyuk Marzia Pasquali Source Type: research

Quantitative analysis of urine acylglycines by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS): reference intervals and disease specific patterns in individuals with organic acidemias and fatty acid oxidation disorders
CONCLUSION: This manuscript extends our previously published method by providing the reference ranges and disease specific increases and patterns of urine acylglycine species using the largest data set published to date.PMID:34634292 | DOI:10.1016/j.cca.2021.10.007 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 11, 2021 Category: Chemistry Authors: Judith A Hobert Irene De Biase Tatiana Yuzyuk Marzia Pasquali Source Type: research

Quantitative analysis of urine acylglycines by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS): reference intervals and disease specific patterns in individuals with organic acidemias and fatty acid oxidation disorders
CONCLUSION: This manuscript extends our previously published method by providing the reference ranges and disease specific increases and patterns of urine acylglycine species using the largest data set published to date.PMID:34634292 | DOI:10.1016/j.cca.2021.10.007 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 11, 2021 Category: Chemistry Authors: Judith A Hobert Irene De Biase Tatiana Yuzyuk Marzia Pasquali Source Type: research

Detection of COPD and Lung Cancer with electronic nose using ensemble learning methods
CONCLUSION: The e-nose system developed with TGS gas sensors was portable, low cost, and gave a rapid response. It has been demonstrated that the VOC profiles of patients with pulmonary diseases and healthy controls are different and hence the e-nose system can be used as a potential diagnostic device for patients with lung diseases.PMID:34627826 | DOI:10.1016/j.cca.2021.10.005 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 10, 2021 Category: Chemistry Authors: Binson V A M Subramoniam Luke Mathew Source Type: research

Analysis of Hair Steroid Hormone Concentrations at Different Parts of the Head by Liquid Chromatography-Tandem Mass Spectrometry
CONCLUSIONS: This research expands upon the role of steroid hormones in hair follicle tissue elucidating their relationship with disease, thus contributing to disease management.PMID:34627827 | DOI:10.1016/j.cca.2021.10.004 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 10, 2021 Category: Chemistry Authors: Yoshitaka Hobo Jun Nishikawa Yoshimichi Miyashiro Akira Fujikata Jun Nishikawa Source Type: research

Lymphocyte Function Based on IFN- γ Secretion Assay May Be A Promising Indicator for Assessing Different Immune Status in Renal Transplant Recipients
CONCLUSIONS: In conclusion, the ability of lymphocyte subsets secreting IFN-γ may provide a promising assessment of immune status in recipients and allow timely modifying immunosuppression.PMID:34626603 | DOI:10.1016/j.cca.2021.10.003 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 9, 2021 Category: Chemistry Authors: Qian-Qian Zhang Ya-Long Xie Wei-Jie Zhang Feng Wang Ying Luo Song Chen Sheng Chang Source Type: research

Lymphocyte Function Based on IFN- γ Secretion Assay May Be A Promising Indicator for Assessing Different Immune Status in Renal Transplant Recipients
CONCLUSIONS: In conclusion, the ability of lymphocyte subsets secreting IFN-γ may provide a promising assessment of immune status in recipients and allow timely modifying immunosuppression.PMID:34626603 | DOI:10.1016/j.cca.2021.10.003 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 9, 2021 Category: Chemistry Authors: Qian-Qian Zhang Ya-Long Xie Wei-Jie Zhang Feng Wang Ying Luo Song Chen Sheng Chang Source Type: research

The utility of whole-exome sequencing in accurate diagnosis of neuromuscular disorders in consanguineous families in Jordan
CONCLUSION: Utilization of WES is helpful to facilitate rapid and accurate NMDs diagnosis, complementing a thorough clinical evaluation. This approach can be invaluable to aid in the identification of genetic risks among consanguineous couples. Subsequently, well-informed genetic counselling and potential individualized treatment can be provided.PMID:34624274 | DOI:10.1016/j.cca.2021.10.001 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 8, 2021 Category: Chemistry Authors: Nidaa A Ababneh Dema Ali Ban Al-Kurdi Raghda Barham Isam K Bsisu Deema Dababseh Sally Arafat Asim N Khanfar Leen Makahleh Abdee T Ryalat Malik Sallam Mohammed El-Khateeb Basil Sharrack Abdalla Awidi Source Type: research

Near-infrared-based hematocrit prediction of dried blood spots: an in-depth evaluation
CONCLUSION: A commercially available NIR set-up was extensively and successfully validated, allowing non-contact Hct prediction of DBS with excellent accuracy and precision. This allows to correct for the Hct-based bias observed in partial-punch DBS analysis and the set-up of blood-plasma conversion factors, increasing the application potential of patient-centric sampling.PMID:34624275 | DOI:10.1016/j.cca.2021.10.002 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 8, 2021 Category: Chemistry Authors: Lisa Delahaye Liesl Heughebaert Christoph L ühr Stijn Lambrecht Christophe P Stove Source Type: research

The utility of whole-exome sequencing in accurate diagnosis of neuromuscular disorders in consanguineous families in Jordan
CONCLUSION: Utilization of WES is helpful to facilitate rapid and accurate NMDs diagnosis, complementing a thorough clinical evaluation. This approach can be invaluable to aid in the identification of genetic risks among consanguineous couples. Subsequently, well-informed genetic counselling and potential individualized treatment can be provided.PMID:34624274 | DOI:10.1016/j.cca.2021.10.001 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 8, 2021 Category: Chemistry Authors: Nidaa A Ababneh Dema Ali Ban Al-Kurdi Raghda Barham Isam K Bsisu Deema Dababseh Sally Arafat Asim N Khanfar Leen Makahleh Abdee T Ryalat Malik Sallam Mohammed El-Khateeb Basil Sharrack Abdalla Awidi Source Type: research

Near-infrared-based hematocrit prediction of dried blood spots: an in-depth evaluation
CONCLUSION: A commercially available NIR set-up was extensively and successfully validated, allowing non-contact Hct prediction of DBS with excellent accuracy and precision. This allows to correct for the Hct-based bias observed in partial-punch DBS analysis and the set-up of blood-plasma conversion factors, increasing the application potential of patient-centric sampling.PMID:34624275 | DOI:10.1016/j.cca.2021.10.002 (Source: International Journal of Clinical Chemistry)
Source: International Journal of Clinical Chemistry - October 8, 2021 Category: Chemistry Authors: Lisa Delahaye Liesl Heughebaert Christoph L ühr Stijn Lambrecht Christophe P Stove Source Type: research

ITGA2 Protein Is Associated with Rheumatoid Arthritis in Chinese and Affects Cellular Function of T Cells
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00344-2. doi: 10.1016/j.cca.2021.09.024. Online ahead of print.ABSTRACTBACKGROUND: We identified proteins significant for rheumatoid arthritis (RA) in peripheral blood mononuclear cells (PBMCs), and to clarify mechanisms mediated by underlying proteins that may involve in the pathogenesis of RA.METHODS: Proteome-wide protein expressions were profiled by employing label-free quantitative proteomics methodology (Easy-nLC1000 and Q-exactive). The t-test was applied to identify differentially expressed proteins (DEP, p≤0.05) between RA case and control samples. Gene Ontology enrichm...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Pei He Bing-Hua Wang Rong-Rong Cao Dong-Cheng Zhu Bing Ge Xu Zhou Long-Fei Wu Shu-Feng Lei Fei-Yan Deng Source Type: research

Measuring serum prothrombin in patients treated with warfarin or direct oral anticoagulants
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00343-0. doi: 10.1016/j.cca.2021.09.023. Online ahead of print.ABSTRACTBACKGROUND: Warfarin therapy influences generation of γ-carboxyglutamyl (Gla) residues in prothrombin, causing reduced coagulation activity. It will leave such inactive prothrombin in serum after clot formation, resulting in serum prothrombin constituting total inactive prothrombin in these patients.METHODS: An ELISA was developed to measure biologically inactive prothrombin in serum, and applied to serum from warfarin therapy causing a decrease in Gla residues or direct oral anticoagulant (DOAC) therapy a...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Akira Kondo Hirokazu Kondo Yoshihisa Nakagawa Hideki Kameyama Hiroyuki Ito Daiki Shimomura Hachiro Yamanishi Noriko Hatanaka Yoshikazu Yamamoto Eri Iwai-Kanai Shuji Matsuo Source Type: research

Antibody response induced by the boost overdose during COVID-19 heterologous prime-boost vaccination strategy
CONCLUSIONS: In spite of the overdosage, this study confirms the efficiency of the BNT162b vaccine in eliciting a sustained antibody response as heterologous boost-vaccine in previously Oxford/AstraZeneca vaccinated recipients, as well as, prime-vaccine in COVID-19 infected receivers. Importantly, the humoral immune response of recipients was not proportional to the vaccine overdose. Nonetheless, we cannot portray a univocal effect of vaccine overdose concerning anti-SARS-CoV-2 antibody response because the values found were highly heterogeneous.PMID:34599902 | PMC:PMC8480142 | DOI:10.1016/j.cca.2021.09.022 (Source: Intern...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Francisco Raposo Giuseppe Lippi Source Type: research

ITGA2 Protein Is Associated with Rheumatoid Arthritis in Chinese and Affects Cellular Function of T Cells
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00344-2. doi: 10.1016/j.cca.2021.09.024. Online ahead of print.ABSTRACTBACKGROUND: We identified proteins significant for rheumatoid arthritis (RA) in peripheral blood mononuclear cells (PBMCs), and to clarify mechanisms mediated by underlying proteins that may involve in the pathogenesis of RA.METHODS: Proteome-wide protein expressions were profiled by employing label-free quantitative proteomics methodology (Easy-nLC1000 and Q-exactive). The t-test was applied to identify differentially expressed proteins (DEP, p≤0.05) between RA case and control samples. Gene Ontology enrichm...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Pei He Bing-Hua Wang Rong-Rong Cao Dong-Cheng Zhu Bing Ge Xu Zhou Long-Fei Wu Shu-Feng Lei Fei-Yan Deng Source Type: research

Measuring serum prothrombin in patients treated with warfarin or direct oral anticoagulants
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00343-0. doi: 10.1016/j.cca.2021.09.023. Online ahead of print.ABSTRACTBACKGROUND: Warfarin therapy influences generation of γ-carboxyglutamyl (Gla) residues in prothrombin, causing reduced coagulation activity. It will leave such inactive prothrombin in serum after clot formation, resulting in serum prothrombin constituting total inactive prothrombin in these patients.METHODS: An ELISA was developed to measure biologically inactive prothrombin in serum, and applied to serum from warfarin therapy causing a decrease in Gla residues or direct oral anticoagulant (DOAC) therapy a...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Akira Kondo Hirokazu Kondo Yoshihisa Nakagawa Hideki Kameyama Hiroyuki Ito Daiki Shimomura Hachiro Yamanishi Noriko Hatanaka Yoshikazu Yamamoto Eri Iwai-Kanai Shuji Matsuo Source Type: research

Antibody response induced by the boost overdose during COVID-19 heterologous prime-boost vaccination strategy
CONCLUSIONS: In spite of the overdosage, this study confirms the efficiency of the BNT162b vaccine in eliciting a sustained antibody response as heterologous boost-vaccine in previously Oxford/AstraZeneca vaccinated recipients, as well as, prime-vaccine in COVID-19 infected receivers. Importantly, the humoral immune response of recipients was not proportional to the vaccine overdose. Nonetheless, we cannot portray a univocal effect of vaccine overdose concerning anti-SARS-CoV-2 antibody response because the values found were highly heterogeneous.PMID:34599902 | PMC:PMC8480142 | DOI:10.1016/j.cca.2021.09.022 (Source: Intern...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Francisco Raposo Giuseppe Lippi Source Type: research

ITGA2 Protein Is Associated with Rheumatoid Arthritis in Chinese and Affects Cellular Function of T Cells
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00344-2. doi: 10.1016/j.cca.2021.09.024. Online ahead of print.ABSTRACTBACKGROUND: We identified proteins significant for rheumatoid arthritis (RA) in peripheral blood mononuclear cells (PBMCs), and to clarify mechanisms mediated by underlying proteins that may involve in the pathogenesis of RA.METHODS: Proteome-wide protein expressions were profiled by employing label-free quantitative proteomics methodology (Easy-nLC1000 and Q-exactive). The t-test was applied to identify differentially expressed proteins (DEP, p≤0.05) between RA case and control samples. Gene Ontology enrichm...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Pei He Bing-Hua Wang Rong-Rong Cao Dong-Cheng Zhu Bing Ge Xu Zhou Long-Fei Wu Shu-Feng Lei Fei-Yan Deng Source Type: research

Measuring serum prothrombin in patients treated with warfarin or direct oral anticoagulants
Clin Chim Acta. 2021 Sep 29:S0009-8981(21)00343-0. doi: 10.1016/j.cca.2021.09.023. Online ahead of print.ABSTRACTBACKGROUND: Warfarin therapy influences generation of γ-carboxyglutamyl (Gla) residues in prothrombin, causing reduced coagulation activity. It will leave such inactive prothrombin in serum after clot formation, resulting in serum prothrombin constituting total inactive prothrombin in these patients.METHODS: An ELISA was developed to measure biologically inactive prothrombin in serum, and applied to serum from warfarin therapy causing a decrease in Gla residues or direct oral anticoagulant (DOAC) therapy a...
Source: International Journal of Clinical Chemistry - October 2, 2021 Category: Chemistry Authors: Akira Kondo Hirokazu Kondo Yoshihisa Nakagawa Hideki Kameyama Hiroyuki Ito Daiki Shimomura Hachiro Yamanishi Noriko Hatanaka Yoshikazu Yamamoto Eri Iwai-Kanai Shuji Matsuo Source Type: research