Efficacy and safety of antibiotics for treatment of leptospirosis: a systematic review and network meta-analysis
ConclusionsFindings recommend that leptospirosis patients be treated with antibiotics, which significantly reduced the leptospirosis defervescence time. Cephalosporins, doxycycline, and penicillin are suggested, and azithromycin may be a suitable alternative for drug-resistant cases.Systematic review registrationPROSPERO CRD42022354938. (Source: Systematic Reviews)
Source: Systematic Reviews - April 16, 2024 Category: International Medicine & Public Health Source Type: research
Doxycycline-induced gastric injury
Hong Kong Med J. 2024 Apr 15. doi: 10.12809/hkmj2310708. Online ahead of print.NO ABSTRACTPMID:38618912 | DOI:10.12809/hkmj2310708 (Source: Hong Kong Med J)
Source: Hong Kong Med J - April 15, 2024 Category: General Medicine Authors: H H W Liu T W Ho N S K Tsang J T W Lam Y T Hui Source Type: research
Doxycycline-induced gastric injury
Hong Kong Med J. 2024 Apr 15. doi: 10.12809/hkmj2310708. Online ahead of print.NO ABSTRACTPMID:38618912 | DOI:10.12809/hkmj2310708 (Source: Hong Kong Medical Journal)
Source: Hong Kong Medical Journal - April 15, 2024 Category: General Medicine Authors: H H W Liu T W Ho N S K Tsang J T W Lam Y T Hui Source Type: research
High-sensitivity concentration detection of trace doxycycline in solution by molecular imprinting based on surface plasmon resonance technology
The abuse of doxycycline (DC) will lead to the presence of its residues in animals and water environments, which threatens human health. It ... (Source: Journal of the Optical Society of America B)
Source: Journal of the Optical Society of America B - April 15, 2024 Category: Physics Authors: Zhitao Yang Shijie Wang Sijia Han Lingzui Wei Source Type: research
Antibiotics Not Associated with Shorter Duration or Reduced Severity of Acute Lower Respiratory Tract Infection
ConclusionsIn this large prospective study in the US primary and urgent care setting, antibiotics had no measurable impact on the severity or duration of cough due to acute LRTI. Patients had unrealistic expectations regarding the duration of LRTI and the effect of antibiotics which should be the target of antibiotic stewardship efforts. (Source: Journal of General Internal Medicine)
Source: Journal of General Internal Medicine - April 15, 2024 Category: Internal Medicine Source Type: research
The transcription factor bmi1 increases hypoxic signaling in oral cavity epithelia
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 8:167161. doi: 10.1016/j.bbadis.2024.167161. Online ahead of print.ABSTRACTThe tongue epithelium is maintained by a proliferative basal layer. This layer contains long-lived stem cells (SCs), which produce progeny cells that move up to the surface as they differentiate. B-lymphoma Mo-MLV insertion region 1 (Bmi1), a protein in mammalian Polycomb Repressive Complex 1 (PRC1) and a biomarker of oral squamous cell carcinoma, is expressed in almost all basal epithelial SCs of the tongue, and single, Bmi1-labelled SCs give rise to cells in all epithelial layers. We previously develope...
Source: Biochimica et Biophysica Acta - April 10, 2024 Category: Biochemistry Authors: Jorge Baquero Xiao-Han Tang Annalisa Ferrotta Tuo Zhang Krysta M DiKun Lorraine J Gudas Source Type: research
The transcription factor bmi1 increases hypoxic signaling in oral cavity epithelia
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 8:167161. doi: 10.1016/j.bbadis.2024.167161. Online ahead of print.ABSTRACTThe tongue epithelium is maintained by a proliferative basal layer. This layer contains long-lived stem cells (SCs), which produce progeny cells that move up to the surface as they differentiate. B-lymphoma Mo-MLV insertion region 1 (Bmi1), a protein in mammalian Polycomb Repressive Complex 1 (PRC1) and a biomarker of oral squamous cell carcinoma, is expressed in almost all basal epithelial SCs of the tongue, and single, Bmi1-labelled SCs give rise to cells in all epithelial layers. We previously develope...
Source: Molecular Medicine - April 10, 2024 Category: Molecular Biology Authors: Jorge Baquero Xiao-Han Tang Annalisa Ferrotta Tuo Zhang Krysta M DiKun Lorraine J Gudas Source Type: research
The transcription factor bmi1 increases hypoxic signaling in oral cavity epithelia
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 8:167161. doi: 10.1016/j.bbadis.2024.167161. Online ahead of print.ABSTRACTThe tongue epithelium is maintained by a proliferative basal layer. This layer contains long-lived stem cells (SCs), which produce progeny cells that move up to the surface as they differentiate. B-lymphoma Mo-MLV insertion region 1 (Bmi1), a protein in mammalian Polycomb Repressive Complex 1 (PRC1) and a biomarker of oral squamous cell carcinoma, is expressed in almost all basal epithelial SCs of the tongue, and single, Bmi1-labelled SCs give rise to cells in all epithelial layers. We previously develope...
Source: Biochimica et Biophysica Acta - April 10, 2024 Category: Biochemistry Authors: Jorge Baquero Xiao-Han Tang Annalisa Ferrotta Tuo Zhang Krysta M DiKun Lorraine J Gudas Source Type: research
Bartonella quintana pulmonary native valve endocarditis
AbstractBartonella quintana is a well-known cause of blood culture-negative endocarditis; however, pulmonary valve involvement is rare. The case of a 40-year-old African male who presented to the Emergency Department with chest pain, cardiac failure, and a 2-week history of fever is presented. Transoesophageal echocardiography confirmed an atrial septal defect, severe pulmonary insufficiency with large vegetations, severe mitral regurgitation due to anterior leaflet prolapse, and right ventricular dysfunction. Empirical antibiotic therapy was started, and urgent surgical intervention was decided. There were vegetations on ...
Source: Indian Journal of Thoracic and Cardiovascular Surgery - April 10, 2024 Category: Cardiovascular & Thoracic Surgery Source Type: research
Extended blood stage sensitivity profiles of < em > Plasmodium cynomolgi < /em > to doxycycline and tafenoquine, as a model for < em > Plasmodium vivax < /em >
Antimicrob Agents Chemother. 2024 Apr 8:e0028024. doi: 10.1128/aac.00280-24. Online ahead of print.ABSTRACTTesting Plasmodium vivax antimicrobial sensitivity is limited to ex vivo schizont maturation assays, which preclude determining the IC50s of delayed action antimalarials such as doxycycline. Using Plasmodium cynomolgi as a model for P. vivax, we determined the physiologically significant delayed death effect induced by doxycycline [IC50(96 h), 1,401 ± 607 nM]. As expected, IC50(96 h) to chloroquine (20.4 nM), piperaquine (12.6 µM), and tafenoquine (1,424 nM) were not affected by extended exposure.PMID:38587391 | DOI...
Source: Antimicrobial Agents and Chemotherapy - April 8, 2024 Category: Microbiology Authors: Peter Christensen Rosy Cinzah Rossarin Suwanarusk Adeline Chiew Yen Chua Osamu Kaneko Dennis E Kyle Htin Lin Aung Jessica Matheson Pablo Bifani Laurent R énia Gregory M Cook Georges Snounou Bruce Russell Source Type: research
Extended blood stage sensitivity profiles of < em > Plasmodium cynomolgi < /em > to doxycycline and tafenoquine, as a model for < em > Plasmodium vivax < /em >
Antimicrob Agents Chemother. 2024 Apr 8:e0028024. doi: 10.1128/aac.00280-24. Online ahead of print.ABSTRACTTesting Plasmodium vivax antimicrobial sensitivity is limited to ex vivo schizont maturation assays, which preclude determining the IC50s of delayed action antimalarials such as doxycycline. Using Plasmodium cynomolgi as a model for P. vivax, we determined the physiologically significant delayed death effect induced by doxycycline [IC50(96 h), 1,401 ± 607 nM]. As expected, IC50(96 h) to chloroquine (20.4 nM), piperaquine (12.6 µM), and tafenoquine (1,424 nM) were not affected by extended exposure.PMID:38587391 | DOI...
Source: Antimicrobial Agents and Chemotherapy - April 8, 2024 Category: Microbiology Authors: Peter Christensen Rosy Cinzah Rossarin Suwanarusk Adeline Chiew Yen Chua Osamu Kaneko Dennis E Kyle Htin Lin Aung Jessica Matheson Pablo Bifani Laurent R énia Gregory M Cook Georges Snounou Bruce Russell Source Type: research
Epsilon-poly-l-lysine microneedle patch loaded with amorphous doxycycline nanoparticles for synergistic treatment of skin infection
Int J Biol Macromol. 2024 Apr 4:131383. doi: 10.1016/j.ijbiomac.2024.131383. Online ahead of print.ABSTRACTThe development of antibiotic-loaded microneedles has been hindered for years by limited excipient options, restricted drug-loading space, poor microneedle formability, and short-term drug retention. Therefore, this study proposes a dissolving microneedle fabricated from the host-defense peptide ε-poly-l-lysine (EPL) as an antibacterial adjuvant system for delivering antibiotics. EPL serves not only as a major matrix material for the microneedle tips, but also as a broad-spectrum antibacterial agent that facilitates ...
Source: International Journal of Biological Macromolecules - April 5, 2024 Category: Biochemistry Authors: Rui Luo Dongyi Xian Feng Li Guiling Zhou Ling Jiang Jieyi Wu Liming Lin Yuwei Zheng Guanyu Liu Qian Xu Xin Pan Chuanbin Wu Tingting Peng Guilan Quan Chao Lu Source Type: research
Generation of Connexin-Expressing Stable Cell Pools
We describe a step-by-step protocol to produce the LV particle by transient transfection and the direct use of the harvested LV stock to generate stable cell pools. We further present step-by-step immunolabeling protocols to characterize the transgene protein expression by confocal microscopy using an antibody that targets an extracellular domain epitope of Cx32 in living cells, and in fixed permeabilized cells using high affinity anti-Cx32 antibodies. Using common molecular biology laboratory techniques, this protocol can be adapted to generate stable pools expressing any transgene of interest, for both in vitro and in vi...
Source: Mol Biol Cell - April 5, 2024 Category: Molecular Biology Authors: Abraham Tettey-Matey Chiara Di Pietro Viola Donati Fabio Mammano Daniela Marazziti Source Type: research
Drug-induced lupus erythematosus in childhood: Case-based review
CONCLUSION: For children presenting with SLE features, proper drug history is crucial since DILE may be more frequent than anticipated. An association of the relevant drug with the symptoms, and resolution of symptoms on drug withdrawal provides evidence for the diagnosis of DILE.PMID:38580326 | DOI:10.1177/09612033241245078 (Source: Lupus)
Source: Lupus - April 5, 2024 Category: Rheumatology Authors: Ummusen Kaya Akca Seher Sener Ezgi Deniz Batu Zeynep Balik Ozge Basaran Yelda Bilginer Seza Ozen Source Type: research
Epsilon-poly-l-lysine microneedle patch loaded with amorphous doxycycline nanoparticles for synergistic treatment of skin infection
Int J Biol Macromol. 2024 Apr 4:131383. doi: 10.1016/j.ijbiomac.2024.131383. Online ahead of print.ABSTRACTThe development of antibiotic-loaded microneedles has been hindered for years by limited excipient options, restricted drug-loading space, poor microneedle formability, and short-term drug retention. Therefore, this study proposes a dissolving microneedle fabricated from the host-defense peptide ε-poly-l-lysine (EPL) as an antibacterial adjuvant system for delivering antibiotics. EPL serves not only as a major matrix material for the microneedle tips, but also as a broad-spectrum antibacterial agent that facilitates ...
Source: International Journal of Biological Macromolecules - April 5, 2024 Category: Biochemistry Authors: Rui Luo Dongyi Xian Feng Li Guiling Zhou Ling Jiang Jieyi Wu Liming Lin Yuwei Zheng Guanyu Liu Qian Xu Xin Pan Chuanbin Wu Tingting Peng Guilan Quan Chao Lu Source Type: research
